Design and construction of Carboxylesterase 2c Gene knockout Rats by CRISPR/Cas9
CONCLUSION: The Ces2c KO rat model was successfully constructed by CRISPR/Cas9 system. This rat model can not only be used as an important tool to study the drug metabolism mediated by Ces2, but also as an important animal model to study the physiological function of Ces2.PMID:36694315 | DOI:10.2174/1389200224666230123140919 (Source: Current Drug Metabolism)
Source: Current Drug Metabolism - January 25, 2023 Category: Drugs & Pharmacology Authors: Jie Liu Xuyang Shang Bingyi Yao Yuanjin Zhang Shengbo Huang Yuanqing Guo Xin Wang Source Type: research
Design and construction of Carboxylesterase 2c Gene knockout Rats by CRISPR/Cas9
CONCLUSION: The Ces2c KO rat model was successfully constructed by CRISPR/Cas9 system. This rat model can not only be used as an important tool to study the drug metabolism mediated by Ces2, but also as an important animal model to study the physiological function of Ces2.PMID:36694315 | DOI:10.2174/1389200224666230123140919 (Source: Current Drug Metabolism)
Source: Current Drug Metabolism - January 25, 2023 Category: Drugs & Pharmacology Authors: Jie Liu Xuyang Shang Bingyi Yao Yuanjin Zhang Shengbo Huang Yuanqing Guo Xin Wang Source Type: research
Design and construction of Carboxylesterase 2c Gene knockout Rats by CRISPR/Cas9
CONCLUSION: The Ces2c KO rat model was successfully constructed by CRISPR/Cas9 system. This rat model can not only be used as an important tool to study the drug metabolism mediated by Ces2, but also as an important animal model to study the physiological function of Ces2.PMID:36694315 | DOI:10.2174/1389200224666230123140919 (Source: Current Drug Metabolism)
Source: Current Drug Metabolism - January 25, 2023 Category: Drugs & Pharmacology Authors: Jie Liu Xuyang Shang Bingyi Yao Yuanjin Zhang Shengbo Huang Yuanqing Guo Xin Wang Source Type: research
Design and construction of Carboxylesterase 2c Gene knockout Rats by CRISPR/Cas9
CONCLUSION: The Ces2c KO rat model was successfully constructed by CRISPR/Cas9 system. This rat model can not only be used as an important tool to study the drug metabolism mediated by Ces2, but also as an important animal model to study the physiological function of Ces2.PMID:36694315 | DOI:10.2174/1389200224666230123140919 (Source: Current Drug Metabolism)
Source: Current Drug Metabolism - January 25, 2023 Category: Drugs & Pharmacology Authors: Jie Liu Xuyang Shang Bingyi Yao Yuanjin Zhang Shengbo Huang Yuanqing Guo Xin Wang Source Type: research
Design and construction of Carboxylesterase 2c Gene knockout Rats by CRISPR/Cas9
CONCLUSION: The Ces2c KO rat model was successfully constructed by CRISPR/Cas9 system. This rat model can not only be used as an important tool to study the drug metabolism mediated by Ces2, but also as an important animal model to study the physiological function of Ces2.PMID:36694315 | DOI:10.2174/1389200224666230123140919 (Source: Current Drug Metabolism)
Source: Current Drug Metabolism - January 25, 2023 Category: Drugs & Pharmacology Authors: Jie Liu Xuyang Shang Bingyi Yao Yuanjin Zhang Shengbo Huang Yuanqing Guo Xin Wang Source Type: research
Clinical outcomes of Medical management options for chronic anal fissures in a long term follow up: systematic review and meta-analysis
Conclusions: Calcium channel blockers performed well regarding the healing of CAF when compared to others in long-term follow-up. The superiority of botulinum toxin was not evidenced compared to topical treatments. More studies are needed to better assess recurrence rates. (Source: Digestive Diseases)
Source: Digestive Diseases - January 16, 2023 Category: Gastroenterology Source Type: research
Comparing the efficacy and safety of different analgesic strategies after open hemorrhoidectomy: a systematic review and network meta-analysis
ConclusionDiltiazem ointment may be the most effective medication for pain relief following open hemorrhoidectomy, and it can dramatically reduce pain within one week of surgery. The second and third recommended medications are BT and sucralfate ointment. GTN has a significant advantage in alleviating pain 24 h after open hemorrhoidectomy, but whether it causes headache is debatable; thus, it should be used with caution. EA’s analgesic efficacy is still unknown. There was limited evidence on the safety of the intervention in this study, and it was simply presented statistically. (Source: International Journal of Colorectal Disease)
Source: International Journal of Colorectal Disease - January 7, 2023 Category: Gastroenterology Source Type: research
A clinically utilized intravenous continuous rate infusion of diltiazem does not significantly decrease systolic function in healthy dogs
Am J Vet Res. 2023 Jan 12:1-8. doi: 10.2460/ajvr.22.09.0158. Online ahead of print.ABSTRACTOBJECTIVE: To determine if left ventricular systolic function on echocardiography, systemic blood pressure, and electrocardiography change with a clinically accepted intravenous (IV) diltiazem constant rate infusion (CRI) compared to a control.ANIMALS: 10 healthy client-owned adult dogs.PROCEDURES: Prospective, masked, crossover study from May 27, 2021, to August 22, 2021. Dogs were randomized to receive diltiazem (loading dose of 240 μg/kg, IV followed by a CRI of 6 μg/kg/min for 300 minutes) or the same volume of 5% dextrose in w...
Source: American Journal of Veterinary Research - January 6, 2023 Category: Veterinary Research Authors: William H Whitehouse Justin D Thomason Dorothy A Thompson-Butler Megan D Kelley Natalia Cernicchiaro Matthew C Tanner Source Type: research
A clinically utilized intravenous continuous rate infusion of diltiazem does not significantly decrease systolic function in healthy dogs
Am J Vet Res. 2023 Jan 12:1-8. doi: 10.2460/ajvr.22.09.0158. Online ahead of print.ABSTRACTOBJECTIVE: To determine if left ventricular systolic function on echocardiography, systemic blood pressure, and electrocardiography change with a clinically accepted intravenous (IV) diltiazem constant rate infusion (CRI) compared to a control.ANIMALS: 10 healthy client-owned adult dogs.PROCEDURES: Prospective, masked, crossover study from May 27, 2021, to August 22, 2021. Dogs were randomized to receive diltiazem (loading dose of 240 μg/kg, IV followed by a CRI of 6 μg/kg/min for 300 minutes) or the same volume of 5% dextrose in w...
Source: American Journal of Veterinary Research - January 6, 2023 Category: Veterinary Research Authors: William H Whitehouse Justin D Thomason Dorothy A Thompson-Butler Megan D Kelley Natalia Cernicchiaro Matthew C Tanner Source Type: research
A clinically utilized intravenous continuous rate infusion of diltiazem does not significantly decrease systolic function in healthy dogs
Am J Vet Res. 2023 Jan 12:1-8. doi: 10.2460/ajvr.22.09.0158. Online ahead of print.ABSTRACTOBJECTIVE: To determine if left ventricular systolic function on echocardiography, systemic blood pressure, and electrocardiography change with a clinically accepted intravenous (IV) diltiazem constant rate infusion (CRI) compared to a control.ANIMALS: 10 healthy client-owned adult dogs.PROCEDURES: Prospective, masked, crossover study from May 27, 2021, to August 22, 2021. Dogs were randomized to receive diltiazem (loading dose of 240 μg/kg, IV followed by a CRI of 6 μg/kg/min for 300 minutes) or the same volume of 5% dextrose in w...
Source: American Journal of Veterinary Research - January 6, 2023 Category: Veterinary Research Authors: William H Whitehouse Justin D Thomason Dorothy A Thompson-Butler Megan D Kelley Natalia Cernicchiaro Matthew C Tanner Source Type: research
A clinically utilized intravenous continuous rate infusion of diltiazem does not significantly decrease systolic function in healthy dogs
Am J Vet Res. 2023 Jan 12:1-8. doi: 10.2460/ajvr.22.09.0158. Online ahead of print.ABSTRACTOBJECTIVE: To determine if left ventricular systolic function on echocardiography, systemic blood pressure, and electrocardiography change with a clinically accepted intravenous (IV) diltiazem constant rate infusion (CRI) compared to a control.ANIMALS: 10 healthy client-owned adult dogs.PROCEDURES: Prospective, masked, crossover study from May 27, 2021, to August 22, 2021. Dogs were randomized to receive diltiazem (loading dose of 240 μg/kg, IV followed by a CRI of 6 μg/kg/min for 300 minutes) or the same volume of 5% dextrose in w...
Source: American Journal of Veterinary Research - January 6, 2023 Category: Veterinary Research Authors: William H Whitehouse Justin D Thomason Dorothy A Thompson-Butler Megan D Kelley Natalia Cernicchiaro Matthew C Tanner Source Type: research
Repetitive multiform narrow complex tachycardia
A 73-year-old man presented to the emergency department with palpitations. After experiencing transient exertional palpitations and dyspnea a few months earlier, investigation revealed moderate left ventricular dysfunction and extensive coronary artery disease with evidence of prior inferior infarction. He underwent bypass grafting and remained symptom free with normalization of his ejection fraction until the current presentation. Figure 1 demonstrates his presenting rhythm that did not respond to adenosine but terminated with intravenous diltiazem. (Source: Heart Rhythm)
Source: Heart Rhythm - January 1, 2023 Category: Cardiology Authors: Christopher X. Wong, Robert M. Hayward, Melvin M. Scheinman Tags: Scheinman/Peter ECG Corner Source Type: research
Can Targeting Sphincter Spasm Reduce Post-Haemorrhoidectomy Pain? A Systematic Review and Meta-Analysis
ConclusionEvidence suggests that lateral sphincterotomy, administration of botulinum toxin and the application of topical diltiazem or GTN can reduce post-operative pain after haemorrhoidectomy. Lateral sphincterotomy should not be routinely used due to the risk of incontinence. (Source: World Journal of Surgery)
Source: World Journal of Surgery - December 31, 2022 Category: Surgery Source Type: research
Glucocorticoid Effects on Working Memory Impairment Require L-Type Calcium Channel Activity Within Prefrontal Cortex
Neurobiol Learn Mem. 2022 Nov 18:107700. doi: 10.1016/j.nlm.2022.107700. Online ahead of print.ABSTRACTPrevious findings have indicated that glucocorticoid hormones impair working memory via an interaction with the β-adrenoceptor-cAMP signaling cascade to rapidly increase cAMP-dependent protein kinase (PKA) activity within the prefrontal cortex (PFC). However, it remains elusive how such activation of PKA can affect downstream cellular mechanisms in regulating PFC cognitive function. PKA is known to activate L-type voltage-gated Ca2+ channels (LTCCs) which regulate a broad range of cellular processes, including neuronal e...
Source: Neurobiology of Learning and Memory - November 21, 2022 Category: Neurology Authors: Areg Barsegyan James L McGaugh Benno Roozendaal Source Type: research
