Cytolethal distending toxin: from genotoxin to a potential biomarker and anti-tumor target
This study discusses the virulence role of CDT, causing pathogenicity by acting as a tri-perditious complex in the CDT-producing species with an emphasis on its potential role as a biomarker and an anti-tumor agent.Graphic abstract (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - August 11, 2021 Category: Microbiology Source Type: research
SERS-based sensor for the detection of sexually transmitted pathogens in the male swab specimens: A new approach for clinical diagnosis
Biosens Bioelectron. 2021 May 21;189:113358. doi: 10.1016/j.bios.2021.113358. Online ahead of print.ABSTRACTThe surface-enhanced Raman scattering (SERS) has been widely tested for its usefulness in microbiological studies, providing many information-rich spectra which are a kind of 'whole-organism fingerprint' and enabling identification of bacterial species. Here we show, previously not considered, the comprehensive SERS-chemometric analysis of five bacterial pathogens, namely Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, and Haemophilus ducreyi, all being responsible for sexual...
Source: Biosensors and Bioelectronics - May 30, 2021 Category: Biotechnology Authors: Sylwia M Berus Monika Adamczyk-Pop ławska Beata M łynarczyk-Bonikowska Evelin Witkowska Tomasz Szymborski Jacek Waluk Agnieszka Kami ńska Source Type: research
SERS-based sensor for the detection of sexually transmitted pathogens in the male swab specimens: A new approach for clinical diagnosis
Biosens Bioelectron. 2021 May 21;189:113358. doi: 10.1016/j.bios.2021.113358. Online ahead of print.ABSTRACTThe surface-enhanced Raman scattering (SERS) has been widely tested for its usefulness in microbiological studies, providing many information-rich spectra which are a kind of 'whole-organism fingerprint' and enabling identification of bacterial species. Here we show, previously not considered, the comprehensive SERS-chemometric analysis of five bacterial pathogens, namely Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, and Haemophilus ducreyi, all being responsible for sexual...
Source: Biosensors and Bioelectronics - May 30, 2021 Category: Biotechnology Authors: Sylwia M Berus Monika Adamczyk-Pop ławska Beata M łynarczyk-Bonikowska Evelin Witkowska Tomasz Szymborski Jacek Waluk Agnieszka Kami ńska Source Type: research
SERS-based sensor for the detection of sexually transmitted pathogens in the male swab specimens: A new approach for clinical diagnosis
Biosens Bioelectron. 2021 May 21;189:113358. doi: 10.1016/j.bios.2021.113358. Online ahead of print.ABSTRACTThe surface-enhanced Raman scattering (SERS) has been widely tested for its usefulness in microbiological studies, providing many information-rich spectra which are a kind of 'whole-organism fingerprint' and enabling identification of bacterial species. Here we show, previously not considered, the comprehensive SERS-chemometric analysis of five bacterial pathogens, namely Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, and Haemophilus ducreyi, all being responsible for sexual...
Source: Biosensors and Bioelectronics - May 30, 2021 Category: Biotechnology Authors: Sylwia M Berus Monika Adamczyk-Pop ławska Beata M łynarczyk-Bonikowska Evelin Witkowska Tomasz Szymborski Jacek Waluk Agnieszka Kami ńska Source Type: research
Brazilian Protocol for Sexually Transmitted Infections, 2020: infections that cause genital ulcers
This article addresses clinical genital ulcer syndrome caused by sexually transmitted infections and its most common etiological agents: Treponema pallidum (syphilis), herpes simplex virus-2 (genital herpes) and herpes simplex virus-1 (perioral herpes), Haemophilus ducreyi (chancroid), Chlamydia trachomatis serotypes L1, L2 and L3 (lymphogranuloma venereum), and Klebsiella granulomatis (donovanosis). Epidemiological and clinical aspects of these infections and guidelines for their diagnosis and treatment are presented, including strategies for surveillance, prevention, and control actions to support health managers and pro...
Source: Revista da Sociedade Brasileira de Medicina Tropical - May 17, 2021 Category: Tropical Medicine Source Type: research
Male circumcision and Sexually transmitted Infections & #8211; An update
Kajal S Mehta, Yogesh S Marfatia, Apexa P Jain, Dhiral J Shah, Disha S BaxiIndian Journal of Sexually Transmitted Diseases and AIDS 2021 42(1):1-6Role of male circumcision (MC) as a tool to prevent sexually transmitted infections (STIs)/human immunodeficiency virus (HIV) was assessed. An attempt was made to search articles related to association between MC and STIs/HIV. A thorough search was carried out to find out quality articles published in indexed specialty journals. Centers for Disease Control and Prevention and World Health Organization (WHO) sites were also referred. Warm and moist environment of area under foreski...
Source: Indian Journal of Sexually Transmitted Diseases - May 4, 2021 Category: Infectious Diseases Authors: Kajal S Mehta Yogesh S Marfatia Apexa P Jain Dhiral J Shah Disha S Baxi Source Type: research
The Active Subunit of the Cytolethal Distending Toxin, CdtB, Derived From Both Haemophilus ducreyi and Campylobacter jejuni Exhibits Potent Phosphatidylinositol-3,4,5-Triphosphate Phosphatase Activity
In this study, we extended our observations to include assessment of Cdts from Haemophilus ducreyi (HdCdt) and Campylobacter jejuni (CjCdt). We now report that the CdtB subunit from HdCdt and CjCdt, similar to that of AaCdt, exhibit potent PIP3 phosphatase activity and that Jurkat cells treated with these Cdts exhibit PI-3K signaling blockade: reduced levels of pAkt and pGSK3β. Since non-phosphorylated GSK3β is the active form of this kinase, we compared Cdts for dependence on GSK3β activity. Two GSK3β inhibitors were employed, LY2090314 and CHIR99021; both inhibitors blocked the ability of Cdts to induce cell cycle ar...
Source: Frontiers in cellular and infection microbiology - March 29, 2021 Category: Microbiology Source Type: research
Ulcerative skin lesions among children in Cameroon: It is not always Yaws
by Jean-Philippe Ndzomo Ngono, Serges Tchatchouang, Mireille Victorine Noah Tsanga, Earnest Njih Tabah, Albert Tchualeu, Kingsley Asiedu, Lorenzo Giacani, Sara Eyangoh, Tania Crucitti
Outbreaks of yaws-like ulcerative skin lesions in children are frequently reported in tropical and sub-tropical countries. The origin of these lesions might be primarily traumatic or infectious; in the latter case,Treponema pallidum subspeciespertenue, the yaws agent, andHaemophilus ducreyi, the agent of chancroid, are two of the pathogens commonly associated with the aetiology of skin ulcers. In this work, we investigated the presence ofT.p...
Source: PLoS Neglected Tropical Diseases - February 16, 2021 Category: Tropical Medicine Authors: Jean-Philippe Ndzomo Ngono Source Type: research
Interactions of the Skin Pathogen Haemophilus ducreyi With the Human Host
The obligate human pathogen Haemophilus ducreyi causes both cutaneous ulcers in children and sexually transmitted genital ulcers (chancroid) in adults. Pathogenesis is dependent on avoiding phagocytosis and exploiting the suppurative granuloma-like niche, which contains a myriad of innate immune cells and memory T cells. Despite this immune infiltrate, long-lived immune protection does not develop against repeated H. ducreyi infections—even with the same strain. Most of what we know about infectious skin diseases comes from naturally occurring infections and/or animal models; however, for H. ducreyi, this information com...
Source: Frontiers in Immunology - February 3, 2021 Category: Allergy & Immunology Source Type: research
Changing trends of sexually transmitted infections and estimation of partner notification at a tertiary care center in North India
Conclusion: In the present study, fungal and viral STIs showed an upward trend, whereas bacterial STIs such as syphilis and chancroid demonstrated a declining trend. The measures to improve PN are urgently required for both cure and the prevention of STIs. (Source: Indian Journal of Sexually Transmitted Diseases)
Source: Indian Journal of Sexually Transmitted Diseases - November 11, 2020 Category: Infectious Diseases Authors: Seema Goel Dimple Chopra Vivek Choudhary Amandeep Riyat Siddharth Chopra Source Type: research
Sexually transmitted infections and human immunodeficiency virus coinfection: Scenario in Western India
Conclusions: The study represents decreasing trends in bacterial STIs and the rise of viral STIs. Atypical presentations of classic STIs were more frequent than non-HIV-infected individuals. (Source: Indian Journal of Sexually Transmitted Diseases)
Source: Indian Journal of Sexually Transmitted Diseases - November 11, 2020 Category: Infectious Diseases Authors: Santoshdev P Rathod Bela Padhiar Bela Shah Source Type: research
Yaws, Haemophilus ducreyi, and Other Bacterial Causes of Cutaneous Ulcer Disease in the South Pacific Islands
Cutaneous ulcers in the tropics are a painful and debilitating condition that anchors people into poverty. In rural regions of the South Pacific, infectious cutaneous ulcers are caused mainly by bacteria, including Treponema pallidum pertenue (yaws), Haemophilus ducreyi, and polymicrobial ulcers. For this group of infections the term cutaneous ulcer disease (CUD) is proposed. Some infections can cause malformations on the bone that have a permanent impact on lives in endemic communities. Better characterization of CUD may help design diagnostic tools and more effective antimicrobial therapies. This review updates the knowl...
Source: Dermatologic Clinics - October 30, 2020 Category: Dermatology Authors: Camila G-Beiras, Maria Ubals, Marc Corbacho-Monn é, Martí Vall-Mayans, Oriol Mitjà Source Type: research
Membrane thinning and lateral gating are consistent features of BamA across multiple species
by Jinchan Liu, James C. Gumbart
In Gram-negative bacteria, the folding and insertion of β-barrel outer membrane proteins (OMPs) to the outer membrane are mediated by the β-barrel assembly machinery (BAM) complex. Two leading models of this process have been put forth: the hybrid barrel model, which claims that a lateral gate i n BamA’s β-barrel can serve as a template for incoming OMPs, and the passive model, which claims that a thinned membrane near the lateral gate of BamA accelerates spontaneous OMP insertion. To examine the key elements of these two models, we have carried out 45.5 μs of equilibrium molecular d...
Source: PLoS Computational Biology - October 28, 2020 Category: Biology Authors: Jinchan Liu Source Type: research
Genital Ulcers: Differential Diagnosis and Management.
Abstract
Genital ulcers may be located on the vagina, penis, and anorectal or perineal areas and may be infectious or noninfectious. Herpes simplex virus is the most common cause of genital ulcers in the United States. A diagnosis of genital herpes simplex virus infection is made through physical examination and observation of genital lesions. The 2015 Centers for Disease Control and Prevention sexually transmitted disease guidelines provide strategies for the management of patients with genital ulcer disease. Specific testing includes a polymerase chain reaction test for herpes simplex virus; syphilis ser...
Source: American Family Physician - March 13, 2020 Category: Primary Care Authors: Roett MA Tags: Am Fam Physician Source Type: research
Evaluation of 2 Commercial Assays for the Detection of Lymphogranuloma Venereum in Rectal Samples
Conclusions
According to the results obtained, both tests could be used for the detection of LGV in rectal samples. (Source: Sexually Transmitted Diseases)
Source: Sexually Transmitted Diseases - February 8, 2020 Category: Sexual Medicine Tags: Original Studies Source Type: research
