Revisiting bacterial cytolethal distending toxin structure and function
Cytolethal distending toxins (CDTs) are intracellular-acting bacterial genotoxins generated by a diverse group of mucocutaneous human pathogens. CDTs must successfully bind to the plasma membrane of host cells in order to exert their modulatory effects. Maximal toxin activity requires all three toxin subunits, CdtA, CdtB, and CdtC, which, based primarily on high-resolution structural data, are believed to preassemble into a tripartite complex necessary for toxin activity. However, biologically active toxin has not been experimentally demonstrated to require assembly of the three subunits into a heterotrimer. Here, we exper...
Source: Frontiers in cellular and infection microbiology - November 14, 2023 Category: Microbiology Source Type: research
Pediatric intensive care unit treatment alters the diversity and composition of the gut microbiota and antimicrobial resistance gene expression in critically ill children
ConclusionThe composition, gene function, and resistance genes of gut microbiota of critically ill children can change significantly after short PICU treatments. Our findings provide a substantial basis for a better understanding of the structure and function of gut microbiota and their role in critical illnesses. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - November 13, 2023 Category: Microbiology Source Type: research
Combined genomic-proteomic approach in the identification of Campylobacter coli amoxicillin-clavulanic acid resistance mechanism in clinical isolates
ConclusionA combined genomics/proteomics approach allowed here to identify a rare putative resistance mechanism associated with amoxicillin-clavulanic acid resistance for C. coli. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - November 9, 2023 Category: Microbiology Source Type: research
Genotypic characterization, antimicrobial susceptibility and virulence determinants of Campylobacter jejuni and Campylobacter coli isolated from pastured poultry farms
In conclusion, the results of this study revealed that Campylobacter isolates from pasture-raised poultry farms showed the ST relatedness to Campylobacter isolates commonly associated with humans, indicating pasture-raised broiler flocks, similar to conventionally-reared broiler flocks, as a potential vector for antibiotic-resistant and pathogenic strains of thermophilic Campylobacter to humans. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - November 9, 2023 Category: Microbiology Source Type: research
Characterization of Campylobacter jejuni proteome profiles in co-incubation scenarios
This study aimed to reveal the influence of co-incubation with Enterococcus faecalis, Enterococcus faecium, or Staphylococcus aureus on the proteome of C. jejuni 81–176 using data-independent-acquisition mass spectrometry (DIA-MS). We compared the proteome profiles during co-incubation with the proteome profile in response to the bile acid deoxycholate (DCA) and investigated the impact of DCA on proteomic changes during co-incubation, as C. jejuni is exposed to both factors during colonization. We identified 1,375 proteins by DIA-MS, which is notably high, approaching the theoretical maximum of 1,645 proteins. S. aureus ...
Source: Frontiers in Microbiology - November 9, 2023 Category: Microbiology Source Type: research
Identification of intestinal and fecal microbial biomarkers using a porcine social stress model
Understanding the relationships between social stress and the gastrointestinal microbiota, and how they influence host health and performance is expected to have many scientific and commercial implementations in different species, including identification and improvement of challenges to animal welfare and health. In particular, the study of the stress impact on the gastrointestinal microbiota of pigs may be of interest as a model for human health. A porcine stress model based on repeated regrouping and reduced space allowance during the last 4 weeks of the finishing period was developed to identify stress-induced changes ...
Source: Frontiers in Microbiology - November 9, 2023 Category: Microbiology Source Type: research
Rapid Genotyping of Campylobacter coli Strains from Poultry Meat by PFGE, Sau-PCR, and fla-DGGE
Curr Microbiol. 2023 Nov 6;80(12):402. doi: 10.1007/s00284-023-03517-4.ABSTRACTThe genotyping of Campylobacter coli was done using three methods, pulsed-field gel electrophoresis (PFGE), Sau-polymerase chain reaction (Sau-PCR), and denaturing gradient gel electrophoresis assay of flagellin gene (fla-DGGE) and the characteristics of these assays were compared. The results showed that a total of 53 strains of C. coli were isolated from chicken and duck samples in three markets. All isolates were clustered into 31, 33, and 15 different patterns with Simpson's index of diversity (SID) values of 0.972, 0.974, and 0.919, respect...
Source: Current Microbiology - November 6, 2023 Category: Microbiology Authors: Ge-Feng Yao Yi-Lin Hu Nian-Qing Kong Jin-Hong Liu Yong-Wen Luo Chu-Yi Li Shui-Lian Bi Source Type: research
Rapid Genotyping of Campylobacter coli Strains from Poultry Meat by PFGE, Sau-PCR, and fla-DGGE
Curr Microbiol. 2023 Nov 6;80(12):402. doi: 10.1007/s00284-023-03517-4.ABSTRACTThe genotyping of Campylobacter coli was done using three methods, pulsed-field gel electrophoresis (PFGE), Sau-polymerase chain reaction (Sau-PCR), and denaturing gradient gel electrophoresis assay of flagellin gene (fla-DGGE) and the characteristics of these assays were compared. The results showed that a total of 53 strains of C. coli were isolated from chicken and duck samples in three markets. All isolates were clustered into 31, 33, and 15 different patterns with Simpson's index of diversity (SID) values of 0.972, 0.974, and 0.919, respect...
Source: Current Microbiology - November 6, 2023 Category: Microbiology Authors: Ge-Feng Yao Yi-Lin Hu Nian-Qing Kong Jin-Hong Liu Yong-Wen Luo Chu-Yi Li Shui-Lian Bi Source Type: research
Genotyping and antibiotic susceptibility of Campylobacter species isolated from raw milk samples in Qazvin, Iran
Campylobacter species are major causes of foodborne illnesses, with unpasteurized milk being a significant carrier of these bacteria, posing a public health risk. One of the challenges in managing Campylobacter i... (Source: BMC Research Notes)
Source: BMC Research Notes - November 6, 2023 Category: Research Authors: Zohreh Ahmadi, Babak Pakbin, Maryam kazemi, Zahra Rahimi and Razzagh Mahmoudi Tags: Research Note Source Type: research
Acute meningitis followed by Campylobacter jejuni associated Guillain-Barr é Syndrome in a pediatric patient with COVID-19: a case report
. (Source: International Journal of Neuroscience)
Source: International Journal of Neuroscience - November 6, 2023 Category: Neuroscience Authors: Alex S. AguirreKelly L. Ter ánDennis Lópeza School of Medicine, Universidad San Francisco de Quito, Quito, Ecuadorb Hospital Metropolitano de Quito, Quito, Ecuador Source Type: research
Detection of multidrug-resistant Campylobacter species from food-producing animals and humans in Nigeria: Public health implications and one health control measures
This study ascertained the phenotypic antimicrobial resistance (AMR) in 56 phenotypically identified TCS from slaughtered cattle, poultry, and humans in Enugu State, Nigeria. The presence of selected AMR and virulence genes harboured by the animal and human isolates were also detected and compared in 36 PCR-confirmed Campylobacter species. All the 56 TCS were multidrug-resistant as none were susceptible to ampicillin, penicillin-G, amoxicillin-clavulanic acid, cephalothin and metronidazole. The isolates were 92.9 %, 62.5 %, 92.9 %, 42.9 %, 26.8 %, 25 %, 28.6 %, 53.7 %, 30.1 %, 32.1 % and 55.4 % resistant to ceftriaxone, na...
Source: Comparative immunology, microbiology and infectious diseases. - November 5, 2023 Category: Allergy & Immunology Authors: Emmanuel O Njoga John A Nwanta Kennedy F Chah Source Type: research
Detection of multidrug-resistant Campylobacter species from food-producing animals and humans in Nigeria: Public health implications and one health control measures
This study ascertained the phenotypic antimicrobial resistance (AMR) in 56 phenotypically identified TCS from slaughtered cattle, poultry, and humans in Enugu State, Nigeria. The presence of selected AMR and virulence genes harboured by the animal and human isolates were also detected and compared in 36 PCR-confirmed Campylobacter species. All the 56 TCS were multidrug-resistant as none were susceptible to ampicillin, penicillin-G, amoxicillin-clavulanic acid, cephalothin and metronidazole. The isolates were 92.9 %, 62.5 %, 92.9 %, 42.9 %, 26.8 %, 25 %, 28.6 %, 53.7 %, 30.1 %, 32.1 % and 55.4 % resistant to ceftriaxone, na...
Source: Comparative immunology, microbiology and infectious diseases. - November 5, 2023 Category: Allergy & Immunology Authors: Emmanuel O Njoga John A Nwanta Kennedy F Chah Source Type: research
Detection of multidrug-resistant Campylobacter species from food-producing animals and humans in Nigeria: Public health implications and one health control measures
This study ascertained the phenotypic antimicrobial resistance (AMR) in 56 phenotypically identified TCS from slaughtered cattle, poultry, and humans in Enugu State, Nigeria. The presence of selected AMR and virulence genes harboured by the animal and human isolates were also detected and compared in 36 PCR-confirmed Campylobacter species. All the 56 TCS were multidrug-resistant as none were susceptible to ampicillin, penicillin-G, amoxicillin-clavulanic acid, cephalothin and metronidazole. The isolates were 92.9 %, 62.5 %, 92.9 %, 42.9 %, 26.8 %, 25 %, 28.6 %, 53.7 %, 30.1 %, 32.1 % and 55.4 % resistant to ceftriaxone, na...
Source: Comparative immunology, microbiology and infectious diseases. - November 5, 2023 Category: Allergy & Immunology Authors: Emmanuel O Njoga John A Nwanta Kennedy F Chah Source Type: research
Detection of multidrug-resistant Campylobacter species from food-producing animals and humans in Nigeria: Public health implications and one health control measures
This study ascertained the phenotypic antimicrobial resistance (AMR) in 56 phenotypically identified TCS from slaughtered cattle, poultry, and humans in Enugu State, Nigeria. The presence of selected AMR and virulence genes harboured by the animal and human isolates were also detected and compared in 36 PCR-confirmed Campylobacter species. All the 56 TCS were multidrug-resistant as none were susceptible to ampicillin, penicillin-G, amoxicillin-clavulanic acid, cephalothin and metronidazole. The isolates were 92.9 %, 62.5 %, 92.9 %, 42.9 %, 26.8 %, 25 %, 28.6 %, 53.7 %, 30.1 %, 32.1 % and 55.4 % resistant to ceftriaxone, na...
Source: Comparative immunology, microbiology and infectious diseases. - November 5, 2023 Category: Allergy & Immunology Authors: Emmanuel O Njoga John A Nwanta Kennedy F Chah Source Type: research
