Subtractive proteomics-guided vaccine targets identification and designing of multi-epitopes vaccine for immune response instigation against Burkholderia pseudomallei
In this study, a methodical workflow using subtractive proteomics, vaccine designing, molecular simulation, and agent-based modeling approaches were used to annotate the whole proteome of Burkholderia pseudomallei (strain K96243) for vaccine designing. Among the total 5717 proteins in the whole proteome, 505 were observed to be essential for the pathogen's survival and pathogenesis predicted by the Database of Essential Genes. Among these, 23 vaccine targets were identified, of which fimbrial assembly chaperone (Q63UH5), Outer membrane protein (Q63UH1), and Hemolysin-like protein (Q63UE4) were selected for the subsequent a...
Source: International Journal of Biological Macromolecules - May 6, 2024 Category: Biochemistry Authors: Fahad M Alshabrmi Eid A Alatawi Source Type: research
Multicenter Investigation of Drug-Resistance in Burkholderia Cepacia Bloodstream Infections in Hebei Province, China, from 2016 to 2021
(Source: Infection and Drug Resistance)
Source: Infection and Drug Resistance - May 3, 2024 Category: Infectious Diseases Tags: Infection and Drug Resistance Source Type: research
Outcomes of Adjunctive Therapy with Open Arthrotomy for Patients with Septic Arthritis Due to Melioidosis: A Retrospective Study
Am J Trop Med Hyg. 2024 Apr 30:tpmd230812. doi: 10.4269/ajtmh.23-0812. Online ahead of print.ABSTRACTMelioidosis is a potentially fatal infection caused by the bacterium Burkholderia pseudomallei. Septic arthritis caused by this infection is uncommon and difficult to treat. The role of adjunctive open arthrotomy in this type of infection has not yet been elucidated. We conducted a retrospective study of patients with microbiologically confirmed melioidosis between January 2002 and December 2022. Patients with a clinical condition of septic arthritis and positive cultures for B. pseudomallei were included. Comparisons were ...
Source: Am J Trop Med Hyg - April 30, 2024 Category: Infectious Diseases Authors: Sorawit Chittrakarn Pisud Siripaitoon Narongdet Kositpantawong Siripen Kanchanasuwan Duangkamol Aiewruengsurat Piyawat Bintachitt Boonsri Charoenmak Monchana Jullangkoon Sarunyou Chusri Source Type: research
Outcomes of Adjunctive Therapy with Open Arthrotomy for Patients with Septic Arthritis Due to Melioidosis: A Retrospective Study
Am J Trop Med Hyg. 2024 Apr 30:tpmd230812. doi: 10.4269/ajtmh.23-0812. Online ahead of print.ABSTRACTMelioidosis is a potentially fatal infection caused by the bacterium Burkholderia pseudomallei. Septic arthritis caused by this infection is uncommon and difficult to treat. The role of adjunctive open arthrotomy in this type of infection has not yet been elucidated. We conducted a retrospective study of patients with microbiologically confirmed melioidosis between January 2002 and December 2022. Patients with a clinical condition of septic arthritis and positive cultures for B. pseudomallei were included. Comparisons were ...
Source: The American Journal of Tropical Medicine and Hygiene - April 30, 2024 Category: Tropical Medicine Authors: Sorawit Chittrakarn Pisud Siripaitoon Narongdet Kositpantawong Siripen Kanchanasuwan Duangkamol Aiewruengsurat Piyawat Bintachitt Boonsri Charoenmak Monchana Jullangkoon Sarunyou Chusri Source Type: research
Outcomes of Adjunctive Therapy with Open Arthrotomy for Patients with Septic Arthritis Due to Melioidosis: A Retrospective Study
Am J Trop Med Hyg. 2024 Apr 30:tpmd230812. doi: 10.4269/ajtmh.23-0812. Online ahead of print.ABSTRACTMelioidosis is a potentially fatal infection caused by the bacterium Burkholderia pseudomallei. Septic arthritis caused by this infection is uncommon and difficult to treat. The role of adjunctive open arthrotomy in this type of infection has not yet been elucidated. We conducted a retrospective study of patients with microbiologically confirmed melioidosis between January 2002 and December 2022. Patients with a clinical condition of septic arthritis and positive cultures for B. pseudomallei were included. Comparisons were ...
Source: Am J Trop Med Hyg - April 30, 2024 Category: Infectious Diseases Authors: Sorawit Chittrakarn Pisud Siripaitoon Narongdet Kositpantawong Siripen Kanchanasuwan Duangkamol Aiewruengsurat Piyawat Bintachitt Boonsri Charoenmak Monchana Jullangkoon Sarunyou Chusri Source Type: research
Outcomes of Adjunctive Therapy with Open Arthrotomy for Patients with Septic Arthritis Due to Melioidosis: A Retrospective Study
Am J Trop Med Hyg. 2024 Apr 30:tpmd230812. doi: 10.4269/ajtmh.23-0812. Online ahead of print.ABSTRACTMelioidosis is a potentially fatal infection caused by the bacterium Burkholderia pseudomallei. Septic arthritis caused by this infection is uncommon and difficult to treat. The role of adjunctive open arthrotomy in this type of infection has not yet been elucidated. We conducted a retrospective study of patients with microbiologically confirmed melioidosis between January 2002 and December 2022. Patients with a clinical condition of septic arthritis and positive cultures for B. pseudomallei were included. Comparisons were ...
Source: The American Journal of Tropical Medicine and Hygiene - April 30, 2024 Category: Tropical Medicine Authors: Sorawit Chittrakarn Pisud Siripaitoon Narongdet Kositpantawong Siripen Kanchanasuwan Duangkamol Aiewruengsurat Piyawat Bintachitt Boonsri Charoenmak Monchana Jullangkoon Sarunyou Chusri Source Type: research
Outcomes of Adjunctive Therapy with Open Arthrotomy for Patients with Septic Arthritis Due to Melioidosis: A Retrospective Study
Am J Trop Med Hyg. 2024 Apr 30:tpmd230812. doi: 10.4269/ajtmh.23-0812. Online ahead of print.ABSTRACTMelioidosis is a potentially fatal infection caused by the bacterium Burkholderia pseudomallei. Septic arthritis caused by this infection is uncommon and difficult to treat. The role of adjunctive open arthrotomy in this type of infection has not yet been elucidated. We conducted a retrospective study of patients with microbiologically confirmed melioidosis between January 2002 and December 2022. Patients with a clinical condition of septic arthritis and positive cultures for B. pseudomallei were included. Comparisons were ...
Source: Am J Trop Med Hyg - April 30, 2024 Category: Infectious Diseases Authors: Sorawit Chittrakarn Pisud Siripaitoon Narongdet Kositpantawong Siripen Kanchanasuwan Duangkamol Aiewruengsurat Piyawat Bintachitt Boonsri Charoenmak Monchana Jullangkoon Sarunyou Chusri Source Type: research
Magnetically modified bacteriophage-triggered ATP release activated EXPAR-CRISPR/Cas14a system for visual detection of Burkholderia pseudomallei
Biosens Bioelectron. 2024 Apr 25;257:116334. doi: 10.1016/j.bios.2024.116334. Online ahead of print.ABSTRACTBurkholderia pseudomallei, widely distributed in tropical and subtropical ecosystems, is capable of causing the fatal zoonotic disease melioidosis and exhibiting a global trend of dissemination. Rapid and sensitive detection of B. pseudomallei is essential for environmental monitoring as well as infection control. Here, we developed an innovative biosensor for quantitatively detecting B. pseudomallei relies on ATP released triggered by bacteriophage-induced bacteria lysis. The lytic bacteriophage vB_BpP_HN01, with hi...
Source: Biosensors and Bioelectronics - April 28, 2024 Category: Biotechnology Authors: Juan Yao Zhang Zhang Hua Pei Ting Zhang Yuping Ruan Chenyuan Liu Yongcan Guo Shuo Gu Qianfeng Xia Source Type: research
Magnetically modified bacteriophage-triggered ATP release activated EXPAR-CRISPR/Cas14a system for visual detection of Burkholderia pseudomallei
Biosens Bioelectron. 2024 Apr 25;257:116334. doi: 10.1016/j.bios.2024.116334. Online ahead of print.ABSTRACTBurkholderia pseudomallei, widely distributed in tropical and subtropical ecosystems, is capable of causing the fatal zoonotic disease melioidosis and exhibiting a global trend of dissemination. Rapid and sensitive detection of B. pseudomallei is essential for environmental monitoring as well as infection control. Here, we developed an innovative biosensor for quantitatively detecting B. pseudomallei relies on ATP released triggered by bacteriophage-induced bacteria lysis. The lytic bacteriophage vB_BpP_HN01, with hi...
Source: Biosensors and Bioelectronics - April 28, 2024 Category: Biotechnology Authors: Juan Yao Zhang Zhang Hua Pei Ting Zhang Yuping Ruan Chenyuan Liu Yongcan Guo Shuo Gu Qianfeng Xia Source Type: research
Magnetically modified bacteriophage-triggered ATP release activated EXPAR-CRISPR/Cas14a system for visual detection of Burkholderia pseudomallei
Biosens Bioelectron. 2024 Apr 25;257:116334. doi: 10.1016/j.bios.2024.116334. Online ahead of print.ABSTRACTBurkholderia pseudomallei, widely distributed in tropical and subtropical ecosystems, is capable of causing the fatal zoonotic disease melioidosis and exhibiting a global trend of dissemination. Rapid and sensitive detection of B. pseudomallei is essential for environmental monitoring as well as infection control. Here, we developed an innovative biosensor for quantitatively detecting B. pseudomallei relies on ATP released triggered by bacteriophage-induced bacteria lysis. The lytic bacteriophage vB_BpP_HN01, with hi...
Source: Biosensors and Bioelectronics - April 28, 2024 Category: Biotechnology Authors: Juan Yao Zhang Zhang Hua Pei Ting Zhang Yuping Ruan Chenyuan Liu Yongcan Guo Shuo Gu Qianfeng Xia Source Type: research
The Predictive Factors Associated with In-Hospital Mortality of Melioidosis: A Cohort Study
Conclusions: Our study identified age, respiration rate, abnormal chest X-ray finding, and CO2 levels are predictive factors associated with in-hospital mortality in melioidosis patients. Physicians should be aware of these factors, have access to aggressive treatment options, and closely monitor patients with these risk factors.PMID:38674300 | DOI:10.3390/medicina60040654 (Source: Medicina (Kaunas))
Source: Medicina (Kaunas) - April 27, 2024 Category: Universities & Medical Training Authors: Sunee Chayangsu Chusana Suankratay Apichat Tantraworasin Jiraporn Khorana Source Type: research
Distribution and survival of pathogens from different waste components and bioaerosol traceability analysis in household garbage room
In this study, a culture method and 16S rRNA high-throughput sequencing were used to determine bacterial communities, culturable pathogens, and human bacterial pathogens (HBPs). The results showed that abundant culturable bacteria were detected in all waste types, and a large number of S. aureus was detected on the surface of recyclable wastes, whereas S. aureus, total coliforms, Salmonella, Enterococcus, and hemolytic bacteria were detected in food waste and other waste. The activities of these detected pathogenic bacteria decreased after 24 h of storage but re-activated within one week. Factors affecting the emergence of...
Source: Environmental Research - April 27, 2024 Category: Environmental Health Authors: Dongsheng Shen Qiang Yu Xiaojing Xing Hening Ding Yuyang Long Cai Hui Source Type: research
The Predictive Factors Associated with In-Hospital Mortality of Melioidosis: A Cohort Study
Conclusions: Our study identified age, respiration rate, abnormal chest X-ray finding, and CO2 levels are predictive factors associated with in-hospital mortality in melioidosis patients. Physicians should be aware of these factors, have access to aggressive treatment options, and closely monitor patients with these risk factors.PMID:38674300 | PMC:PMC11052379 | DOI:10.3390/medicina60040654 (Source: Medicina (Kaunas))
Source: Medicina (Kaunas) - April 27, 2024 Category: Universities & Medical Training Authors: Sunee Chayangsu Chusana Suankratay Apichat Tantraworasin Jiraporn Khorana Source Type: research
Distribution and survival of pathogens from different waste components and bioaerosol traceability analysis in household garbage room
In this study, a culture method and 16S rRNA high-throughput sequencing were used to determine bacterial communities, culturable pathogens, and human bacterial pathogens (HBPs). The results showed that abundant culturable bacteria were detected in all waste types, and a large number of S. aureus was detected on the surface of recyclable wastes, whereas S. aureus, total coliforms, Salmonella, Enterococcus, and hemolytic bacteria were detected in food waste and other waste. The activities of these detected pathogenic bacteria decreased after 24 h of storage but re-activated within one week. Factors affecting the emergence of...
Source: Environmental Research - April 27, 2024 Category: Environmental Health Authors: Dongsheng Shen Qiang Yu Xiaojing Xing Hening Ding Yuyang Long Cai Hui Source Type: research
The Predictive Factors Associated with In-Hospital Mortality of Melioidosis: A Cohort Study
Conclusions: Our study identified age, respiration rate, abnormal chest X-ray finding, and CO2 levels are predictive factors associated with in-hospital mortality in melioidosis patients. Physicians should be aware of these factors, have access to aggressive treatment options, and closely monitor patients with these risk factors.PMID:38674300 | PMC:PMC11052379 | DOI:10.3390/medicina60040654 (Source: Medicina (Kaunas))
Source: Medicina (Kaunas) - April 27, 2024 Category: Universities & Medical Training Authors: Sunee Chayangsu Chusana Suankratay Apichat Tantraworasin Jiraporn Khorana Source Type: research
