Management of unexpected laboratory exposure to Burkholderia pseudomallei
We report here a couple of microbiological arguments that can usually guide microbiologists towards presumptive identification of B. pseudomallei. This case report shows the importance of MALDI-TOF MS accurate databases to ensure accurate microbial identification and antibiotic prophylaxis adapted to individuals who were exposed. We also want to underline the importance of developing an effective strategy of prevention against any accidental exposure that can occur in a microbiological laboratory.PMID:38391168 | DOI:10.1684/abc.2023.1854 (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - February 23, 2024 Category: Biochemistry Authors: Imane Mostaghat Carine Couzigou Beno ît Pilmis Nathalie Castreau Eric Raymond Anne-Lise Maixant Alban Le Monnier Anne Depeille Olivier Gorg é Assaf Mizrahi Source Type: research
Management of unexpected laboratory exposure to Burkholderia pseudomallei
We report here a couple of microbiological arguments that can usually guide microbiologists towards presumptive identification of B. pseudomallei. This case report shows the importance of MALDI-TOF MS accurate databases to ensure accurate microbial identification and antibiotic prophylaxis adapted to individuals who were exposed. We also want to underline the importance of developing an effective strategy of prevention against any accidental exposure that can occur in a microbiological laboratory.PMID:38391168 | DOI:10.1684/abc.2023.1854 (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - February 23, 2024 Category: Biochemistry Authors: Imane Mostaghat Carine Couzigou Beno ît Pilmis Nathalie Castreau Eric Raymond Anne-Lise Maixant Alban Le Monnier Anne Depeille Olivier Gorg é Assaf Mizrahi Source Type: research
Management of unexpected laboratory exposure to Burkholderia pseudomallei
We report here a couple of microbiological arguments that can usually guide microbiologists towards presumptive identification of B. pseudomallei. This case report shows the importance of MALDI-TOF MS accurate databases to ensure accurate microbial identification and antibiotic prophylaxis adapted to individuals who were exposed. We also want to underline the importance of developing an effective strategy of prevention against any accidental exposure that can occur in a microbiological laboratory.PMID:38391168 | DOI:10.1684/abc.2023.1854 (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - February 23, 2024 Category: Biochemistry Authors: Imane Mostaghat Carine Couzigou Beno ît Pilmis Nathalie Castreau Eric Raymond Anne-Lise Maixant Alban Le Monnier Anne Depeille Olivier Gorg é Assaf Mizrahi Source Type: research
Management of unexpected laboratory exposure to Burkholderia pseudomallei
We report here a couple of microbiological arguments that can usually guide microbiologists towards presumptive identification of B. pseudomallei. This case report shows the importance of MALDI-TOF MS accurate databases to ensure accurate microbial identification and antibiotic prophylaxis adapted to individuals who were exposed. We also want to underline the importance of developing an effective strategy of prevention against any accidental exposure that can occur in a microbiological laboratory.PMID:38391168 | DOI:10.1684/abc.2023.1854 (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - February 23, 2024 Category: Biochemistry Authors: Imane Mostaghat Carine Couzigou Beno ît Pilmis Nathalie Castreau Eric Raymond Anne-Lise Maixant Alban Le Monnier Anne Depeille Olivier Gorg é Assaf Mizrahi Source Type: research
Management of unexpected laboratory exposure to Burkholderia pseudomallei
We report here a couple of microbiological arguments that can usually guide microbiologists towards presumptive identification of B. pseudomallei. This case report shows the importance of MALDI-TOF MS accurate databases to ensure accurate microbial identification and antibiotic prophylaxis adapted to individuals who were exposed. We also want to underline the importance of developing an effective strategy of prevention against any accidental exposure that can occur in a microbiological laboratory.PMID:38391168 | DOI:10.1684/abc.2023.1854 (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - February 23, 2024 Category: Biochemistry Authors: Imane Mostaghat Carine Couzigou Beno ît Pilmis Nathalie Castreau Eric Raymond Anne-Lise Maixant Alban Le Monnier Anne Depeille Olivier Gorg é Assaf Mizrahi Source Type: research
Decoding seasonal changes: soil parameters and microbial communities in tropical dry deciduous forests
This study aimed to investigate the influence of seasonal fluctuations on soil characteristics and microbial populations. The soil moisture content dramatically increases in the summer. However, the soil pH only gradually shifts from acidic to slightly neutral. During the summer, electrical conductivity (EC) values range from 0.62 to 1.03 ds m-1, in contrast to their decline in the winter. The levels of soil macronutrients and micronutrients increase during the summer, as does the quantity of soil organic carbon (SOC). A two-way ANOVA analysis reveals limited impacts of seasonal fluctuations and specific geographic locatio...
Source: Frontiers in Microbiology - February 19, 2024 Category: Microbiology Source Type: research
Effectiveness of Umonium38 against Burkholderia pseudomallei, Escherichia coli, Pseudomonas aeruginosa and Methicillin-Resistant Staphylococcus aureus (MRSA)
We investigated the antibacterial efficacy of Umonium38 and Virkon ® against Burkholderia pseudomallei, Escherichia coli, Pseudomonas aeruginosa and Methicillin-Resistant Staphylococcus aureus (MRSA) up to 14 days... (Source: BMC Infectious Diseases)
Source: BMC Infectious Diseases - February 16, 2024 Category: Infectious Diseases Authors: Soiratchaneekorn Ruanchaiman, Premjit Amornchai, Vanaporn Wuthiekanun, Sayan Langla, Peerapol Maroongruang, Khanh Kim Le and Stuart D. Blacksell Tags: Research Source Type: research
Evaluation of the production and extraction of polyhydroxybutyrate from volatile fatty acids and using mixed cultures of PTAR and B. cepacia
Environ Res. 2024 Feb 13:118448. doi: 10.1016/j.envres.2024.118448. Online ahead of print.ABSTRACTThe global consumption of plastics generates accelerated environmental pollution in landfills and marine ecosystems. Biopolymers are the materials with the greatest potential to replace synthetic polymers in the market due to their good biodegradability, however, there are still several disadvantages, mainly related to their production cost. Considering the above, the generation of biodegradable and biocompatible bioplastics stands out as an alternative solution, some of which are made from renewable raw materials, including p...
Source: Environmental Research - February 15, 2024 Category: Environmental Health Authors: Jeniffer Gracia Armando Espinosa Nubia Moreno Iv án Cabeza Source Type: research
Evaluation of the production and extraction of polyhydroxybutyrate from volatile fatty acids and using mixed cultures of PTAR and B. cepacia
Environ Res. 2024 Feb 13:118448. doi: 10.1016/j.envres.2024.118448. Online ahead of print.ABSTRACTThe global consumption of plastics generates accelerated environmental pollution in landfills and marine ecosystems. Biopolymers are the materials with the greatest potential to replace synthetic polymers in the market due to their good biodegradability, however, there are still several disadvantages, mainly related to their production cost. Considering the above, the generation of biodegradable and biocompatible bioplastics stands out as an alternative solution, some of which are made from renewable raw materials, including p...
Source: Environmental Research - February 15, 2024 Category: Environmental Health Authors: Jeniffer Gracia Armando Espinosa Nubia Moreno Iv án Cabeza Source Type: research
Decoration of Burkholderia Hcp1 protein to virus-like particles as a vaccine delivery platform
Infection and Immunity, Ahead of Print. (Source: Infection and Immunity)
Source: Infection and Immunity - February 14, 2024 Category: Infectious Diseases Authors: Nittaya KhakhumNoe Baruch-TorresJacob L. StocktonItziar Chapartegui-Gonz álezAlexander J. BadtenAwadalkareem AdamTian WangAlejandro Huerta-SaqueroY. Whitney YinAlfredo G. Torres1Department of Microbiology & Immunology, University of Texas Medical Branch Source Type: research
Drug eluting bioactive glass ceramics for fusion in spondylodiscitis: a pilot study
AbstractRetrospective observational study. To determine the efficacy and safety of bioactive glass ceramics mixed with autograft in the treatment of spondylodiscitis. Thirty-four patients with spondylodiscitis underwent surgery using autologous bone graft augmented by antibiotic loaded bioactive glass ceramic granules. Twenty-five patients aging 6 to 77, completed 1-year follow-up. The lumbosacral junction was affected in 3, lumbar spine in 13, one each in the dorso-lumbar junction and sacrum, and 7 dorsal spines. The organism isolated wasMycobacterium tuberculosis in 15,Methicillin sensitive Staphylococcus aureus (MSSA) i...
Source: Neurosurgical Review - February 14, 2024 Category: Neurosurgery Source Type: research
Characterization of a novel high-efficiency cracking Burkholderia gladiolus phage vB_BglM_WTB and its application in black fungus
In this study, a phage was identified to control B. gladiolus. The novel phage vB_BglM_WTB (WTB), which lyse B. gladiolus with high efficiency, was isolated from sewage of Huaihe Road Throttle Well Sewage Treatment Plant in Hefei. Transmission electron microscopy showed that WTB had an icosahedral head (69 ± 2 nm) and a long retractable tail (108 ± 2 nm). Its optimal temperature and pH ranges to control B. gladiolus were 25 °C -65 °C and 3-11 respectively. The phage WTB was identified as a linear double-stranded DNA phage of 68, 541 bp with 60.04 % G + C content, with a long latent period of 60 min. Phylogenetic analys...
Source: International Journal of Food Microbiology - February 7, 2024 Category: Food Science Authors: Ting Wang Bin Cheng Rui Jiao Xiyan Zhang Diwei Zhang Xiangyu Cheng Na Ling Yingwang Ye Source Type: research
Genomic editing in < em > Burkholderia multivorans < /em > by CRISPR/Cas9
Appl Environ Microbiol. 2024 Feb 2:e0225023. doi: 10.1128/aem.02250-23. Online ahead of print.ABSTRACTBurkholderia cepacia complex bacteria have emerged as opportunistic pathogens in patients with cystic fibrosis and immunocompromised individuals, causing life-threatening infections. Because of the relevance of these microorganisms, genetic manipulation is crucial for explaining the genetic mechanisms leading to pathogenesis. Despite the availability of allelic exchange tools to obtain unmarked gene deletions in Burkholderia, these require a step of merodiploid formation and another of merodiploid resolution through two in...
Source: Applied and Environmental Microbiology - February 1, 2024 Category: Microbiology Authors: Mirela R Ferreira Vasco Queiroga Leonilde M Moreira Source Type: research
Burkholderia pseudomallei Bacteria in Ornamental Fish Tanks, Vientiane, Laos, 2023
T. Venkatesan et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - February 1, 2024 Category: Infectious Diseases Source Type: research
Crystal structure and biophysical characterization of IspD from Burkholderia thailandensis and Mycobacterium paratuberculosis
The methylerythritol phosphate (MEP) pathway is a metabolic pathway that produces the isoprenoids isopentyl pyrophosphate and dimethylallyl pyrophosphate. Notably, the MEP pathway is present in bacteria and not in mammals, which makes the enzymes of the MEP pathway attractive targets for discovering new anti-infective agents due to the reduced chances of off-target interactions leading to side effects. There are seven enzymes in the MEP pathway, the third of which is IspD. Two crystal structures of Burkholderia thailandensis IspD (BtIspD) were determined: an apo structure and that of a complex with cytidine triphosphate (C...
Source: Acta Crystallographica Section F - January 31, 2024 Category: Biochemistry Authors: Pierce, P.G. Hartnett, B.E. Laughlin, T.M. Blain, J.M. Mayclin, S.J. Bolejack, M.J. Myers, J.B. Higgins, T.W. Dranow, D.M. Sullivan, A. Lorimer, D.D. Edwards, T.E. Hagen, T.J. Horn, J.R. Myler, P.J. Tags: IspD Burkholderia thailandensis Mycobacterium paratuberculosis transferases cytidylyltransferases structural genomics Seattle Structural Genomics Center for Infectious Disease SSGCID research communications Source Type: research
