Isolation, identification, and pathogenicity analysis of newly emerging gosling astrovirus in South China
In this study, we collected 94 gout samples from goose farms in the Guangdong Province of South China. Among them, 87 samples (92.6%) tested positive for GoAstV, out of which five GoAstV strains were isolated after four generations of blind transmission through healthy 13-day-old goose embryos. The whole genome of the isolates was sequenced and further analyzed by comparing the sequences with published sequences from China and other parts of the world. The results of the alignment analysis showed that nucleotide sequence similarities among the five GoAstV isolates were around 97.4–98.8%, 98.6–100%, 98.1–99.8%, and 96...
Source: Frontiers in Microbiology - February 27, 2023 Category: Microbiology Source Type: research
Identification and characterization of a novel avian nephritis virus variant in chickens with enteritis in Hunan province, China
In this study, an ANV strain designated as HNU-ANV-ML-2020 was identified in tissue samples collected from chickens with severe enteritis on a poultry farm in Hunan province, China, and analyzed. The genome of HNU-ANV-ML-2020 is 6943 nucleotides in length. It showed the highest sequence identity (88.1%) to ANV strain CHN/GXJL815/2017 (MN732559) from Guangxi province, China, while it showed less than 86% identity to other astrovirus (AstV) genome sequences available in the GenBank database. The capsid protein of this virus showed the highest sequence identity to ANV strains HQ330482 and HQ330498 from the UK (81.2% and 81.06...
Source: Archives of Virology - January 7, 2023 Category: Virology Source Type: research
A comprehensive molecular survey of viral pathogens associated with canine gastroenteritis
This study highlights the need for routine prophylactic vaccination with the appropriate vaccines. Notably, 70.3% of animals vaccinated with DHPPiL were found to be positive for at least one virus. Hence, regular molecular analysis of t he prevalent viruses is crucial for addressing vaccination failures. (Source: Archives of Virology)
Source: Archives of Virology - January 7, 2023 Category: Virology Source Type: research
Viruses, Vol. 15, Pages 41: Clinical and Epidemiologic Features of Viral Gastroenteritis in Hospitalized Children: An 11-Year Surveillance in Palermo (Sicily)
This study increases general knowledge of VAGE epidemiology and contributes to suggest some a priori diagnostic criteria that could help clinicians to identify and treat viral agents responsible for gastroenteritis in hospital settings. (Source: Viruses)
Source: Viruses - December 22, 2022 Category: Virology Authors: Emanuele Amodio Simona De Grazia Dario Genovese Floriana Bonura Chiara Filizzolo Antonella Collura Francesca Di Bernardo Giovanni M. Giammanco Tags: Article Source Type: research
Diversity of human astroviruses in Germany 2018 and 2019
Aim of this study was to investigate the molecular diversity of human astroviruses (HAstV) in Germany. A follow-up study was performed with human stool samples collected in 2018 –2019, which were genotyped retr... (Source: Virology Journal)
Source: Virology Journal - December 21, 2022 Category: Virology Authors: Sandra Niendorf, Andreas Mas Marques, Claus-Thomas Bock and Sonja Jacobsen Tags: Brief Report Source Type: research
Viruses, Vol. 14, Pages 2820: A Novel Coronavirus and a Broad Range of Viruses in Kenyan Cave Bats
Conclusions: Further monitoring of bats across Kenya is essential to facilitate early recognition of possibly emergent zoonotic viruses. (Source: Viruses)
Source: Viruses - December 17, 2022 Category: Virology Authors: Joseph Kamau Koray Ergunay Paul W. Webala Silvia A. Justi Brian P. Bourke Maureen W. Kamau James Hassell Mary N. Chege David K. Mwaura Cynthia Simiyu Sospeter Kibiwot Samson Onyuok Laura Caicedo-Quiroga Tao Li Dawn M. Zimmerman Yvonne-Marie Linton Tags: Article Source Type: research
Viruses, Vol. 14, Pages 2806: Isolation and Pathogenicity of a Novel Goose Astrovirus from Overfed Adult Landaise Geese in China
ndong Bao
Cun Zhang
Goose astrovirus (GAstV) is an important pathogen causing visceral gout and high mortality in goslings, which has broken out and spread across China. In 2021, a disease characterized by urate deposition on the visceral surface and 30% mortality occurred in commercial adult Landaise geese in Zhejiang Province, China. A systematic study identified an infecting astrovirus, designated ZJCX, that was efficiently isolated from a diseased goose with a chicken hepatocellular carcinoma cell line (LMH). In contrast to other GAstVs originating from goslings, ZJCX caused cytopathogenic effects in LMH cells, a...
Source: Viruses - December 15, 2022 Category: Virology Authors: Yinchu Zhu Hongyu Wang Jionggang Hua Weicheng Ye Liu Chen Zheng Ni Tao Yun Jiale Ma Huochun Yao Endong Bao Cun Zhang Tags: Article Source Type: research
A peptide-based ELISA for detection of antibodies against novel goose astrovirus type 1
In this study, one novel peptide of 627-646 aa in the ORF2 recognized by monoclonal antibody (mAb) 6C6 was used as an antigen to develop an efficient peptide-based ELISA (pELISA) for detection of antibodies against GAstV-1. Specificity analysis showed that the pELISA only reacted with sera against GAstV-1, but not with sera against other pathogens tested. The sensitivity of the pELISA in detecting positive sera was higher than that of the IFA (Indirect immunofluorescence assay). The coefficients of variation (CV) of the intra-assay and inter-assay were both <10%, indicating that the reproducibility of pELISA was good. F...
Source: Journal of Virological Methods - November 10, 2022 Category: Virology Authors: Dan Ren Xinyun Zhang Wei Zhang Mingjun Lian Xianchen Meng Tuofan Li Quan Xie Hongxia Shao Zhimin Wan Aijian Qin Wei Gao Jianqiang Ye Source Type: research
A peptide-based ELISA for detection of antibodies against novel goose astrovirus type 1
In this study, one novel peptide of 627-646 aa in the ORF2 recognized by monoclonal antibody (mAb) 6C6 was used as an antigen to develop an efficient peptide-based ELISA (pELISA) for detection of antibodies against GAstV-1. Specificity analysis showed that the pELISA only reacted with sera against GAstV-1, but not with sera against other pathogens tested. The sensitivity of the pELISA in detecting positive sera was higher than that of the IFA (Indirect immunofluorescence assay). The coefficients of variation (CV) of the intra-assay and inter-assay were both <10%, indicating that the reproducibility of pELISA was good. F...
Source: Journal of Virological Methods - November 10, 2022 Category: Virology Authors: Dan Ren Xinyun Zhang Wei Zhang Mingjun Lian Xianchen Meng Tuofan Li Quan Xie Hongxia Shao Zhimin Wan Aijian Qin Wei Gao Jianqiang Ye Source Type: research
Development of an indirect competitive ELISA method based on ORF2 detecting the antibodies of novel goose astrovirus
J Virol Methods. 2022 Nov 1:114643. doi: 10.1016/j.jviromet.2022.114643. Online ahead of print.ABSTRACTGoose astrovirus (GAstV) characterized by articular and visceral gout, is an emerging pathogen with a wide distribution on mainland China, leading to serious economic losses in the goose-raising industry. Because vaccines to prevent GAstV infections are not available currently, early diagnosis is critical when treating symptomatic geese and in preventing GAstV transmission. In this context, a highly sensitive indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a monoclonal antibody (mAb) to detect G...
Source: Journal of Virological Methods - November 4, 2022 Category: Virology Authors: Dalin He Min Sun Xiaoning Jiang Shuai Zhang Feng Wei Bingrong Wu Youxiang Diao Yi Tang Source Type: research
Development of an indirect competitive ELISA method based on ORF2 detecting the antibodies of novel goose astrovirus
J Virol Methods. 2022 Nov 1:114643. doi: 10.1016/j.jviromet.2022.114643. Online ahead of print.ABSTRACTGoose astrovirus (GAstV) characterized by articular and visceral gout, is an emerging pathogen with a wide distribution on mainland China, leading to serious economic losses in the goose-raising industry. Because vaccines to prevent GAstV infections are not available currently, early diagnosis is critical when treating symptomatic geese and in preventing GAstV transmission. In this context, a highly sensitive indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a monoclonal antibody (mAb) to detect G...
Source: Journal of Virological Methods - November 4, 2022 Category: Virology Authors: Dalin He Min Sun Xiaoning Jiang Shuai Zhang Feng Wei Bingrong Wu Youxiang Diao Yi Tang Source Type: research
Development of an indirect competitive ELISA method based on ORF2 detecting the antibodies of novel goose astrovirus
J Virol Methods. 2022 Nov 2;311:114643. doi: 10.1016/j.jviromet.2022.114643. Online ahead of print.ABSTRACTGoose astrovirus (GAstV) characterized by articular and visceral gout, is an emerging pathogen with a wide distribution on mainland China, leading to serious economic losses in the goose-raising industry. Because vaccines to prevent GAstV infections are not available currently, early diagnosis is critical when treating symptomatic geese and in preventing GAstV transmission. In this context, a highly sensitive indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a monoclonal antibody (mAb) to dete...
Source: Journal of Virological Methods - November 4, 2022 Category: Virology Authors: Dalin He Min Sun Xiaoning Jiang Shuai Zhang Feng Wei Bingrong Wu Youxiang Diao Yi Tang Source Type: research
Incidence, Etiology, and Healthcare Utilization for Acute Gastroenteritis in the Community, United States
Emerg Infect Dis. 2022 Nov;28(11):2234-2242. doi: 10.3201/eid2811.220247.ABSTRACTKnowledge of the epidemiology of sporadic acute gastroenteritis (AGE) in the United States is limited. During September 2016-September 2017, we surveyed Kaiser Permanente Northwest members in Oregon and Washington, USA, to collect data on the 30-day prevalence of dually defined AGE and diarrhea disease and related health-seeking behavior; from a subset of participants, we obtained a stool specimen. Using the iterative proportional fitting algorithm with raked weights, we generated AGE prevalence and annualized rate estimates. We detected norov...
Source: Emerging Infectious Diseases - October 26, 2022 Category: Infectious Diseases Authors: Mark A Schmidt Holly C Groom Andreea M Rawlings Claire P Mattison Suzanne B Salas Rachel M Burke Ben D Hallowell Laura E Calderwood Judy Donald Neha Balachandran Aron J Hall Source Type: research
Incidence, Etiology, and Healthcare Utilization for Acute Gastroenteritis in the Community, United States
Emerg Infect Dis. 2022 Nov;28(11):2234-2242. doi: 10.3201/eid2811.220247.ABSTRACTKnowledge of the epidemiology of sporadic acute gastroenteritis (AGE) in the United States is limited. During September 2016-September 2017, we surveyed Kaiser Permanente Northwest members in Oregon and Washington, USA, to collect data on the 30-day prevalence of dually defined AGE and diarrhea disease and related health-seeking behavior; from a subset of participants, we obtained a stool specimen. Using the iterative proportional fitting algorithm with raked weights, we generated AGE prevalence and annualized rate estimates. We detected norov...
Source: Emerging Infectious Diseases - October 26, 2022 Category: Infectious Diseases Authors: Mark A Schmidt Holly C Groom Andreea M Rawlings Claire P Mattison Suzanne B Salas Rachel M Burke Ben D Hallowell Laura E Calderwood Judy Donald Neha Balachandran Aron J Hall Source Type: research
Incidence, Etiology, and Healthcare Utilization for Acute Gastroenteritis in the Community, United States
Emerg Infect Dis. 2022 Nov;28(11):2234-2242. doi: 10.3201/eid2811.220247.ABSTRACTKnowledge of the epidemiology of sporadic acute gastroenteritis (AGE) in the United States is limited. During September 2016-September 2017, we surveyed Kaiser Permanente Northwest members in Oregon and Washington, USA, to collect data on the 30-day prevalence of dually defined AGE and diarrhea disease and related health-seeking behavior; from a subset of participants, we obtained a stool specimen. Using the iterative proportional fitting algorithm with raked weights, we generated AGE prevalence and annualized rate estimates. We detected norov...
Source: Emerging Infectious Diseases - October 26, 2022 Category: Infectious Diseases Authors: Mark A Schmidt Holly C Groom Andreea M Rawlings Claire P Mattison Suzanne B Salas Rachel M Burke Ben D Hallowell Laura E Calderwood Judy Donald Neha Balachandran Aron J Hall Source Type: research
