Viruses, Vol. 16, Pages 439: First Detection of Benign Rabbit Caliciviruses in Chile
In this study, liver and duodenum rabbit samples from central Chile were tested for the presence of lagoviruses and positive samples were subject to whole RNA sequencing and subsequent data analysis. Phylogenetic analysis revealed a novel RCV variant in duodenal samples that likely originated from European RCVs. Sequencing analysis also detected the presence of a rabbit astrovirus in one of the lagovirus-positive samples. (Source: Viruses)
Source: Viruses - March 12, 2024 Category: Virology Authors: Elena Smertina Luca M. Keller Nina Huang Gabriela Flores-Benner Jennifer Paola Correa-Cuadros Melanie Duclos Fabian M. Jaksic Crist óbal Briceño Victor Neira Ramirez Miguel D íaz-Gacitúa Sebasti án Carrasco-Fernández Ina L. Smith Tanja Strive Maria Tags: Article Source Type: research

Epidemiological investigation and pathogenicity analysis of waterfowl astroviruses in some areas of China
In this study, we collected 1546 waterfowl samples from 13 provinces in China for epidemiological investigation. The results showed that 260 samples (16.8%) were positive. Four species of astrovirus were detected in 13 provinces except Fujian province. Among the four sites tested, the highest positive rates were found in farms and slaughterhouses. Cross-host and mixed infection were observed in four species of waterfowl astroviruses. The whole genome of 17 isolates was sequenced and compared with published sequences. Genetic evolution and homology analysis showed that the isolated strains had high similarity to their refer...
Source: Frontiers in Microbiology - March 11, 2024 Category: Microbiology Source Type: research

Diversity of astroviruses in wild animals in Yunnan province, China
Astroviruses (AstVs) are single-stranded RNA viruses that have been detected in a wide range of mammals and birds. They are associated with numerous interspecies transmissions and viral recombination events, p... (Source: Virology Journal)
Source: Virology Journal - February 27, 2024 Category: Virology Authors: Xingyu Huang, Junjie Hou, Xiang Le, Yutong Hou, Lingsi Yang, Qian Li, Binghui Wang and Xueshan Xia Tags: Research Source Type: research

Development of a fast and sensitive RT-qPCR assay based on SYBR ® green for diagnostic and quantification of Avian Nephritis Virus (ANV) in chickens affected with enteric disease
Enteric viruses are among the most prominent etiological agents of Runting-Stunting Syndrome (RSS). The Avian Nephritis Virus (ANV) is an astrovirus associated with enteric diseases in poultry, whose early dia... (Source: BMC Veterinary Research)
Source: BMC Veterinary Research - January 30, 2024 Category: Veterinary Research Authors: Anthony Loor-Giler, Sara Castillo-Reyes, Silvana Santander-Parra, Manuel Caza, Nikolaos C. Kyriakidis, Antonio J. Piantino Ferreira and Luis Nu ñez Tags: Research Source Type: research

Prevalence of astrovirus and sapovirus among adult oncology patients with acute gastroenteritis using a multiplexed gastrointestinal pathogen PCR panel
ConclusionIn oncology patients with viral gastroenteritis, astrovirus and sapovirus were the causative agents in 18% of the cases. Both viruses were associated with mild disease. Prolonged diarrhea and viral shedding were observed in a few transplant patients. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - January 13, 2024 Category: Microbiology Source Type: research

Viruses, Vol. 16, Pages 109: Metagenomic Analysis of Viromes of Aedes Mosquitoes across India
eep Sarah Cherian Metagenomic analysis of Aedes aegypti and Ae. albopictus mosquitoes from diverse geographical regions of India revealed the presence of several insect viruses of human interest. Most abundant reads found in Ae. aegypti mosquitoes were of Phasi Charoen-like virus (PCLV), Choristoneura fumiferana granulovirus (CfGV), Cell fusing agent virus (CFAV), and Wenzhou sobemo-like virus 4 (WSLV4), whereas WSLV4 and CfGV constituted the highest percentage of reads in Ae. albopictus viromes. Other reads that were of low percentage included Hubei mosquito virus 2 (HMV2), Porcine astrovirus 4 (PAstV4), and Wild Bo...
Source: Viruses - January 12, 2024 Category: Virology Authors: Abhranil Gangopadhayya Kavita Lole Onkar Ghuge Ashwini Ramdasi Asmita Kamble Diya Roy Shivani Thakar Amol Nath AB Sudeep Sarah Cherian Tags: Article Source Type: research

Environmental Surveillance of Human Astroviruses in Jinan City of China, 2020 –2021
AbstractHuman astroviruses (HAstVs) are a significant etiological agent of acute gastroenteritis in children. In order to investigate the circulation of HAstVs during the COVID-19 pandemic, a 2-year environmental surveillance was conducted in Jinan between 2020 and 2021. A total of 24 sewage samples were collected and concentrated. Real-time PCR indicated a positive rate of 83.3%, 79.2% (19/24), and 62.5% for classic, MLB, and VA types of HAstV in sewage samples, respectively, with genomic copies ranging from 6.4  × 103 to 3.7  × 107, 3.2  × 104 to 2.2  × 106, and 1.2  × 104 to 1.6  × 107 lâˆ...
Source: Food and Environmental Virology - January 11, 2024 Category: Virology Source Type: research

Comparison of dye-based and probe-based RT-LAMP in detection of canine astrovirus
In this study, two real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays with high sensitivity, rapidity, and reliability were developed using fluorescence dye and FRET-based assimilating probes for real-time detection of CaAstV. These assays specifically amplified the ORF2 gene of CaAstV and did not amplify any sequences from canine enterovirus. The limit of detection (LOD) of both the probe-based and dye-based RT-LAMPs was 100 copies/ μL. Fluorescence signals were generated within 30 min for the lowest concentration of a standard RNA sample, which was significantly faster than that achi...
Source: Archives of Virology - January 9, 2024 Category: Virology Source Type: research

Temporal changes in the positivity rate of common enteric viruses among paediatric admissions in coastal Kenya, during the COVID-19 pandemic, 2019 –2022
ConclusionOur study finds that in 2020 the transmission of common enteric viruses, especially RVA and astrovirus, in Kilifi Kenya may have been disrupted due to the COVID-19 NPIs. After 2020, local enteric virus transmission patterns appeared to return to pre-pandemic levels coinciding with the removal of most of the government COVID-19 NPIs. (Source: Gut Pathogens)
Source: Gut Pathogens - January 4, 2024 Category: Microbiology Source Type: research

Viruses, Vol. 16, Pages 80: Prevalence of Astroviruses in Different Animal Species in Poland
This study involved 166 intestine samples and pools of spleen, lymph node, and kidney samples collected from 352 wild animals, 52 pigs, and 31 companion animals. Astroviruses were detected in the intestine samples and were separately detected in pools of tissue samples prepared for individual animals using a heminested RT-PCR protocol. Amplicons were subjected to Sanger sequencing, and a phylogenetic analysis of 320 nt RNA-dependent RNA polymerase (RdRp) fragments referring to known nt sequences of astroviruses was performed. Astroviral RNA was detected in the intestine samples and/or tissue pools of red foxes (nine positi...
Source: Viruses - January 4, 2024 Category: Virology Authors: Konrad Kuczera Anna Or łowska Marcin Smreczak Maciej Frant Pawe ł Trębas Jerzy Rola Tags: Article Source Type: research

High prevalence of Enterovirus E, Bovine Kobuvirus, and Astrovirus revealed by viral metagenomics in fecal samples from cattle in Central Colombia
This study showcases the utility of using metagenomic sequencing techniques in epidemiological surveillance. It also paves the way for further research on the influence of these agents on bovine health and their frecuency across the country.PMID:38135265 | DOI:10.1016/j.meegid.2023.105543 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - December 22, 2023 Category: Genetics & Stem Cells Authors: Juli án Esteban Medina Sergio Casta ñeda Luisa P áez-Triana Milena Camargo Diego J Garcia-Corredor Marcela G ómez Nicolas Luna Angie L Ram írez Mart ín Pulido-Medellín Marina Mu ñoz Juan David Ram írez Source Type: research

High prevalence of Enterovirus E, Bovine Kobuvirus, and Astrovirus revealed by viral metagenomics in fecal samples from cattle in Central Colombia
This study showcases the utility of using metagenomic sequencing techniques in epidemiological surveillance. It also paves the way for further research on the influence of these agents on bovine health and their frecuency across the country.PMID:38135265 | DOI:10.1016/j.meegid.2023.105543 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - December 22, 2023 Category: Genetics & Stem Cells Authors: Juli án Esteban Medina Sergio Casta ñeda Luisa P áez-Triana Milena Camargo Diego J Garcia-Corredor Marcela G ómez Nicolas Luna Angie L Ram írez Mart ín Pulido-Medellín Marina Mu ñoz Juan David Ram írez Source Type: research

High prevalence of Enterovirus E, Bovine Kobuvirus, and Astrovirus revealed by viral metagenomics in fecal samples from cattle in Central Colombia
This study showcases the utility of using metagenomic sequencing techniques in epidemiological surveillance. It also paves the way for further research on the influence of these agents on bovine health and their frecuency across the country.PMID:38135265 | DOI:10.1016/j.meegid.2023.105543 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - December 22, 2023 Category: Genetics & Stem Cells Authors: Juli án Esteban Medina Sergio Casta ñeda Luisa P áez-Triana Milena Camargo Diego J Garcia-Corredor Marcela G ómez Nicolas Luna Angie L Ram írez Mart ín Pulido-Medellín Marina Mu ñoz Juan David Ram írez Source Type: research

High prevalence of Enterovirus E, Bovine Kobuvirus, and Astrovirus revealed by viral metagenomics in fecal samples from cattle in Central Colombia
This study showcases the utility of using metagenomic sequencing techniques in epidemiological surveillance. It also paves the way for further research on the influence of these agents on bovine health and their frecuency across the country.PMID:38135265 | DOI:10.1016/j.meegid.2023.105543 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - December 22, 2023 Category: Genetics & Stem Cells Authors: Juli án Esteban Medina Sergio Casta ñeda Luisa P áez-Triana Milena Camargo Diego J Garcia-Corredor Marcela G ómez Nicolas Luna Angie L Ram írez Mart ín Pulido-Medellín Marina Mu ñoz Juan David Ram írez Source Type: research

High prevalence of Enterovirus E, Bovine Kobuvirus, and Astrovirus revealed by viral metagenomics in fecal samples from cattle in Central Colombia
This study showcases the utility of using metagenomic sequencing techniques in epidemiological surveillance. It also paves the way for further research on the influence of these agents on bovine health and their frecuency across the country.PMID:38135265 | DOI:10.1016/j.meegid.2023.105543 (Source: Infection, Genetics and Evolution)
Source: Infection, Genetics and Evolution - December 22, 2023 Category: Genetics & Stem Cells Authors: Juli án Esteban Medina Sergio Casta ñeda Luisa P áez-Triana Milena Camargo Diego J Garcia-Corredor Marcela G ómez Nicolas Luna Angie L Ram írez Mart ín Pulido-Medellín Marina Mu ñoz Juan David Ram írez Source Type: research