Viruses, Vol. 14, Pages 2806: Isolation and Pathogenicity of a Novel Goose Astrovirus from Overfed Adult Landaise Geese in China

Viruses, Vol. 14, Pages 2806: Isolation and Pathogenicity of a Novel Goose Astrovirus from Overfed Adult Landaise Geese in China Viruses doi: 10.3390/v14122806 Authors: Yinchu Zhu Hongyu Wang Jionggang Hua Weicheng Ye Liu Chen Zheng Ni Tao Yun Jiale Ma Huochun Yao Endong Bao Cun Zhang Goose astrovirus (GAstV) is an important pathogen causing visceral gout and high mortality in goslings, which has broken out and spread across China. In 2021, a disease characterized by urate deposition on the visceral surface and 30% mortality occurred in commercial adult Landaise geese in Zhejiang Province, China. A systematic study identified an infecting astrovirus, designated ZJCX, that was efficiently isolated from a diseased goose with a chicken hepatocellular carcinoma cell line (LMH). In contrast to other GAstVs originating from goslings, ZJCX caused cytopathogenic effects in LMH cells, and the crystalline arrangement of viral particles was observed through transmission electron microscopy. Indeed, phylogenetic analysis and nucleotide homology comparison revealed that ZJCX isolate belongs to the genotype II cluster of GAstVs and displays 97.8–98.4% identity with other GAstV II strains. However, several specific mutations occurred in the polyprotein and capsid protein regions. Moreover, a pathogenicity assessment of ZJCX with a gosling model was conducted, and typical visceral gout was reproduced and led to 18% mortality. The viral loads of ZJCX ...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research