Monitoring Human Viral Pathogens Reveals Potential Hazard for Treated Wastewater Discharge or Reuse
Wastewater discharge to the environment or its reuse after sanitization poses a concern for public health given the risk of transmission of human viral diseases. However, estimating the viral infectivity along the wastewater cycle presents technical challenges and still remains underexplored. Recently, human-associated crAssphage has been investigated to serve as viral pathogen indicator to monitor fecal impacted water bodies, even though its assessment as biomarker for infectious enteric viruses has not been explored yet. To this end, the occurrence of potentially infectious norovirus genogroup I (GI), norovirus GII, hepa...
Source: Frontiers in Microbiology - April 8, 2022 Category: Microbiology Source Type: research
First report of canine Astrovirus and Sapovirus in Greece, hosting both asymptomatic and gastroenteritis symptomatic dogs
Astrovirus, Norovirus and Sapovirus are widely distributed viruses in humans and animals worldwide. They have frequently been associated with disease, mainly of gastroenteric nature. In dogs, these viruses hav... (Source: Virology Journal)
Source: Virology Journal - March 31, 2022 Category: Virology Authors: Efthymia Stamelou, Ioannis A. Giantsis, Konstantinos V. Papageorgiou, Evanthia Petridou, Irit Davidson, Zoe S. Polizop οulou, Anna Papa and Spyridon K. Kritas Tags: Research Source Type: research
Viruses, Vol. 14, Pages 722: An Insight into the Molecular Characteristics and Associated Pathology of Chicken Astroviruses
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The chicken astrovirus (CAstV) is a ubiquitous enteric RNA virus that has been associated mainly with conditions, such as the runting-stunting syndrome, severe kidney disease, visceral gout, and white chick syndrome, in broiler-type chickens worldwide. Sequence analysis of the capsid genes’ amino acids of the strains involved in these conditions reveals a genetic relationship and diversity between and within the CAstV genogroups and subgroups based on phylogenetic analysis, genetic distance (p-dist), and pathogenicity. While the two genogroups (A and B) are demarcated phylogenetically, their pairwise ...
Source: Viruses - March 30, 2022 Category: Virology Authors: Abdullahi Abdullahi Raji Abdul Rahman Omar Tags: Review Source Type: research
Core antigenic advantage domain-based ELISA to detect antibody against novel goose astrovirus in breeding geese
In conclusion, the developed indirect ELISA is simple, specific, and sensitive, which will be greatly useful to screen GAstV infection and block vertical transmission. KEY POINTS: • B-cell epitopes of GAstV capsid protein were predicted and expressed as immunogen • A core antigenic advantage domain-based ELISA was established to detect GAstV-specific antibody • The established ELISA will contribute to inspection and elimination of infected breeding geese and provide a useful tool for large scale serological testing of GAstV in geese.PMID:35254499 | DOI:10.1007/s00253-022-11852-y (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - March 7, 2022 Category: Microbiology Authors: Zeng Wang Huayuan Chen Shenyan Gao Mingzhen Song Zicong Shi Zhifeng Peng Qianyue Jin Li Zhao Hongxing Qiao Chuanzhou Bian Xia Yang Xiaozhan Zhang Jun Zhao Source Type: research
Viruses, Vol. 14, Pages 513: Development and Large-Scale Testing of a Novel One-Step Triplex RT-qPCR Assay for Simultaneous Detection of & ldquo;Neurotropic & rdquo; Porcine Sapeloviruses, Teschoviruses (Picornaviridae) and Type 3 Porcine Astroviruses (Astroviridae) in Various Samples including Nasal Swabs
In this study, a novel highly sensitive one-step triplex RT-qPCR assay was introduced which can detect all known types of neurotropic sapelo-, tescho- and type 3 astroviruses in multiple types of samples of swine. The assay was evaluated using in vitro synthesized RNA standards and a total of 142 archived RNA samples including known sapelo-, tescho- and type 3 astrovirus positive and negative CNS, enteric and nasal specimens. The results of a large-scale epidemiological investigation of these viruses on n = 473 nasal swab samples from n = 28 industrial-type swine farms in Hungary indicate that all three neurotropic viruses...
Source: Viruses - March 2, 2022 Category: Virology Authors: Zolt án László P éter Pankovics G ábor Reuter Attila Cs ágola Korn élia Bodó G ábor Gáspár Mih ály Albert Hunor B író Ákos Boros Tags: Article Source Type: research
Prevalence and seasonal pattern of enteric viruses among hospitalized children with acute gastroenteritis in Samawah, Iraq
This study included children admitted to the surgical care, aged 6 months to 5 years, who suffered from acute gastroenteritis and received treatment at Samawah, Iraq, from December 2018 to December 2019. Test results detected different types of rotaviruses, adenoviruses, astroviruses using ELISA. 56.6% of the infections were attributed to a viral pathogen. The main cause was attributed to rotavirus and adenovirus. The causative agents of diarrheal diseases in 28.1% of cases are rotaviruses, in 17.05% - adenoviruses, in 11.43% - astroviruses. Viral mono-infections are detected more often than mixed infections. Viral intesti...
Source: Journal of Medicine and Life - February 21, 2022 Category: General Medicine Authors: Hazim Talib Thwiny Nawar Jasim Alsalih Zeayd Fadhil Saeed Ali Mosa Rashid Al-Yasari Moyed Abd AlHussein Al-Saadawe Mohenned Abd ElHussein Alsaadawi Source Type: research
