Metatranscriptomic assessment of diarrhoeic faeces reveals diverse RNA viruses in rotavirus group A infected piglets and calves from India
RNA viruses are a major group contributing to emerging infectious diseases and neonatal diarrhoea, causing morbidity and mortality in humans and animals. Hence, the present study investigated the metatranscriptomic-derived faecal RNA virome in rotavirus group A (RVA)-infected diarrheic piglets and calves from India. The viral genomes retrieved belonged to Astroviridae in both species, while Reoviridae and Picornaviridae were found only in piglets. The nearly complete genomes of porcine RVA (2), astrovirus (AstV) (6), enterovirus G (EVG) (2), porcine sapelovirus (PSV) (2), Aichivirus C (1), and porcine teschovirus (PTV) (1)...
Source: Frontiers in cellular and infection microbiology - October 27, 2023 Category: Microbiology Source Type: research
Molecular Characterization of Gastroenteric Viruses in Wastewater from Cities in Uruguay
AbstractGroup A Rotavirus, Human Astrovirus, and Norovirus (RVA, HAstV, and NoV) are recognized as the major causative agents of acute gastroenteritis in children and adults worldwide. The aim of this study was to determine the prevalence and molecular epidemiology of RVA, HAstV, and NoV in wastewater from three cities in Uruguay. Thirty-six samples from Bella Uni ón, Salto, and Fray Bentos cities were analyzed using quantitative and qualitative PCR. RVA was the most frequently detected virus (50%), followed by HAstV (39%), NoV GII (36%), and NoV GI (25%). RVA strains were characterized as P[8] and G3 based on the VP4 and...
Source: Food and Environmental Virology - October 24, 2023 Category: Virology Source Type: research
Entry and egress of human astroviruses
Adv Virus Res. 2023;117:81-119. doi: 10.1016/bs.aivir.2023.08.001. Epub 2023 Sep 7.ABSTRACTAstroviruses encapsidate a positive-sense, single-stranded RNA genome into ∼30nm icosahedral particles that infect a wide range of mammalian and avian species, but their biology is not well understood. Human astroviruses (HAstV) are divided into three clades: classical HAstV serotypes 1-8, and novel or non-classical HAstV of the MLB and VA clades. These viruses are part of two genogroups and phylogenetically cluster with other mammalian astroviruses, highlighting their zoonotic potential. HAstV are a highly prevalent cause of nonba...
Source: Cell Research - October 13, 2023 Category: Cytology Authors: Pedro Soares Porto Andres Rivera Rootjikarn Moonrinta Christiane E Wobus Source Type: research
Localization and distribution of goose astrovirus 2 antigens in different tissues at different times
Goose astrovirus 2 (GAstV-2) causes visceral gout in goslings and has resulted in significant economic losses in the goose industry of China since its outbreak in 2017. To further investigate the distribution ... (Source: BMC Veterinary Research)
Source: BMC Veterinary Research - September 23, 2023 Category: Veterinary Research Authors: Feng Wei, Xiaoning Jiang, Dalin He, Youxiang Diao and Yi Tang Tags: Research Source Type: research
Universal primer multiplex PCR assay for detection and genotyping of porcine astroviruses
J Virol Methods. 2023 Sep 18:114822. doi: 10.1016/j.jviromet.2023.114822. Online ahead of print.ABSTRACTPorcine astroviruses (PAstV) are members of the family Astroviridae, Mamastravirus genus and have been identified to have five genotypes (PAstV1-5). These viruses are highly prevalent in pigs and can cause enteric disease as well as neurological or respiratory symptoms depending on their genotypes. At present, the epidemiological impacts of some PAstV genotypes on pigs are largely unknown and hence continuously monitoring of these PAstVs may be needed. The purpose of this research was to develop an improved and efficient...
Source: Journal of Virological Methods - September 20, 2023 Category: Virology Authors: Qiuya Zhang Dan Wen Qin Liu Tanja Opriessnig Xiaoya Yu Yonghou Jiang Source Type: research
Universal primer multiplex PCR assay for detection and genotyping of porcine astroviruses
J Virol Methods. 2023 Sep 18;322:114822. doi: 10.1016/j.jviromet.2023.114822. Online ahead of print.ABSTRACTPorcine astroviruses (PAstV) are members of the family Astroviridae, Mamastravirus genus and have been identified to have five genotypes (PAstV1-5). These viruses are highly prevalent in pigs and can cause enteric disease as well as neurological or respiratory symptoms depending on their genotypes. At present, the epidemiological impacts of some PAstV genotypes on pigs are largely unknown and hence continuously monitoring of these PAstVs may be needed. The purpose of this research was to develop an improved and effic...
Source: Journal of Virological Methods - September 20, 2023 Category: Virology Authors: Qiuya Zhang Dan Wen Qin Liu Tanja Opriessnig Xiaoya Yu Yonghou Jiang Source Type: research
Universal primer multiplex PCR assay for detection and genotyping of porcine astroviruses
J Virol Methods. 2023 Sep 18;322:114822. doi: 10.1016/j.jviromet.2023.114822. Online ahead of print.ABSTRACTPorcine astroviruses (PAstV) are members of the family Astroviridae, Mamastravirus genus and have been identified to have five genotypes (PAstV1-5). These viruses are highly prevalent in pigs and can cause enteric disease as well as neurological or respiratory symptoms depending on their genotypes. At present, the epidemiological impacts of some PAstV genotypes on pigs are largely unknown and hence continuously monitoring of these PAstVs may be needed. The purpose of this research was to develop an improved and effic...
Source: Journal of Virological Methods - September 20, 2023 Category: Virology Authors: Qiuya Zhang Dan Wen Qin Liu Tanja Opriessnig Xiaoya Yu Yonghou Jiang Source Type: research
Universal primer multiplex PCR assay for detection and genotyping of porcine astroviruses
J Virol Methods. 2023 Sep 18:114822. doi: 10.1016/j.jviromet.2023.114822. Online ahead of print.ABSTRACTPorcine astroviruses (PAstV) are members of the family Astroviridae, Mamastravirus genus and have been identified to have five genotypes (PAstV1-5). These viruses are highly prevalent in pigs and can cause enteric disease as well as neurological or respiratory symptoms depending on their genotypes. At present, the epidemiological impacts of some PAstV genotypes on pigs are largely unknown and hence continuously monitoring of these PAstVs may be needed. The purpose of this research was to develop an improved and efficient...
Source: Journal of Virological Methods - September 20, 2023 Category: Virology Authors: Qiuya Zhang Dan Wen Qin Liu Tanja Opriessnig Xiaoya Yu Yonghou Jiang Source Type: research
Coinfection of porcine astrovirus and other porcine viruses in diarrheic pigs in Haryana, India
In this study, 306 rectal swabs from diarrheal pigs of various ages (0-3 weeks, 3-6 weeks, and>6 weeks) were collected from 54 piggery units in different climatic zones in Haryana state, India. These samples were tested for the presence of porcine astrovirus (PAstV), porcine rotavirus group A (PRV-A), and classical swine fever virus (CSFV) by reverse transcription polymerase chain reaction (RT-PCR), and porcine circovirus 2 (PCV-2) by polymerase chain reaction (PCR). Out of the 306 samples tested, 153 (50%), 108 (35.3%), 32 (10.6%), and three (0.9%) tested positive for PAstV, PCV-2, PRV-A, and CSFV, respectively. A sing...
Source: Archives of Virology - September 7, 2023 Category: Virology Source Type: research
GSE240869 Astrovirus replication is dependent on induction of double membrane vesicles through a PI3K-dependent, LC3-independent pathway
Contributors : Shaoyuan Tan ; Theresa Bub ; Ana Vazquez-Pagan ; Tim FlerlageSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe cellular response to astrovirus infection is not well defined. We used single cell RNA sequencing (scRNA-seq) to determine cellular response to astrovirus early or late in infection. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - August 20, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Viruses, Vol. 15, Pages 1679: Detection and Molecular Characterization of Animal Adenovirus and Astrovirus from Western Maharashtra, India
a Lavania
Astroviruses (AstV) and adenoviruses (AdV) are associated with diarrhoea in young animals. However, the epidemiology and genetic diversity of AstVs and AdVs in animals is not well studied. Hence, the present study was conducted to detect and characterize AstVs and AdVs in calves, piglets and puppies from Western Maharashtra, India. Out of the processed porcine (48), canine (80), and bovine (65) faecal samples, the porcine AstV (PAstV), bovine AstV (BAstV), canine AstV (CAstV), and porcine AdV (PAdV) were detected in 12.5%, 7.69%, 3.75% and 4.1% of samples, respectively. In the RNA-dependent RNA polymerase reg...
Source: Viruses - August 1, 2023 Category: Virology Authors: Pradeep M. Sawant Rishabh B. Waghchaure Pooja A. Shinde Avani P. Palikondawar Mallika Lavania Tags: Brief Report Source Type: research
Gammaretroviruses, novel viruses and pathogenic bacteria in Australian bats with neurological signs, pneumonia and skin lesions
Virology. 2023 Jul 17;586:43-55. doi: 10.1016/j.virol.2023.07.011. Online ahead of print.ABSTRACTMore than 70 bat species are found in mainland Australia. While most studies of bat viromes focus on sampling seemingly healthy individuals, little is known about the viruses and bacteria associated with diseased bats. We performed traditional diagnostic techniques and metatranscriptomic sequencing on tissue samples from 43 Australian bats, comprising three flying fox (Pteropodidae) and two microbat species experiencing a range of disease syndromes, including mass mortality, neurological signs, pneumonia and skin lesions. Of no...
Source: Virology - July 24, 2023 Category: Virology Authors: Kate Van Brussel Jackie E Mahar Jane Hall Hannah Bender Ayda Susana Ortiz-Baez Wei-Shan Chang Edward C Holmes Karrie Rose Source Type: research
Epidemiology of gastrointestinal infections: lessons learned from syndromic testing, Region Zealand, Denmark
AbstractThe aim of this study was to investigate the value of syndromic diagnostic testing for a better understanding of the epidemiology of gastrointestinal infections in Denmark. Here we evaluated the QIAstat-Dx ® Gastrointestinal (GI) Panel 1 assay on 18,610 fecal samples requested for analysis for enteric pathogens in Region Zealand, Denmark, in 1 year (October 1, 2021, to September 30, 2022). In total, 6905 (37%) samples were detected positive for one or more diarrhoeal bacteria, viruses, and protozoa. The most common bacterial, viral, and parasitic pathogens detected with the QIAstat-Dx® Gastrointestinal Panel 1 we...
Source: European Journal of Clinical Microbiology and Infectious Diseases - July 19, 2023 Category: Microbiology Source Type: research
Attenuation hotspots in neurotropic human astroviruses
by Hashim Ali, Aleksei Lulla, Alex S. Nicholson, Jack Hankinson, Elizabeth B. Wignall-Fleming, Rhian L. O ’Connor, Diem-Lan Vu, Stephen C. Graham, Janet E. Deane, Susana Guix, Valeria Lulla
During the last decade, the detection of neurotropic astroviruses has increased dramatically. The MLB genogroup of astroviruses represents a genetically distinct group of zoonotic astroviruses associated with gastroenteritis and severe neurological complications in young children, the immunocompromised, and the elderly. Using different virus evolution approaches, we identified dispensable regions in the 3 ′ end of the capsid-coding...
Source: PLoS Biology: Archived Table of Contents - July 17, 2023 Category: Biology Authors: Hashim Ali Source Type: research
Viruses, Vol. 15, Pages 1488: Detection and Genetic Characterization of Astroviruses in Brain Tissues of Wild Raccoon Dogs
ae-Ku Oem
Astroviruses (AstVs) have been detected in a wide range of animal species, including mammals and birds. Recently, a novel AstV associated with neurological symptoms has been detected in the brains of some mammals. Raccoon dog AstV has been reported recently in China. However, there have been no reports in South Korea. Therefore, the present study aimed to detect and genetically characterize AstVs in the intestine and brain tissues of 133 wild raccoon dogs collected in Korea between 2017 and 2019. Of the seven raccoon dogs, AstVs were detected in six intestinal tissues and four brain tissues. Analysis of the c...
Source: Viruses - June 30, 2023 Category: Virology Authors: Su-Beom Chae Chang-Gi Jeong Jun-Soo Park Eun-Jee Na Jae-Ku Oem Tags: Article Source Type: research
