Molecular epidemiology and virulence of goose astroviruses genotype-2 with different internal gene sequences
Goose astrovirus (GAstV) is a small, non-enveloped, single-stranded, positive-sense RNA virus. GAstV has rapidly spread across various regions in China since 2016. In Sichuan, out of 113 samples were collected from goose diseases between 2019 and 2022, 97 were positive for GAstV through PCR testing. Remarkably, over the past three years, GAstV outbreak in Sichuan has accounted for an astonishing 85.8% of all goose-origin viruses. Among these cases, 63.9% had single GAstV infections, 29.9% had dual infections, and 6.2% had quadruple infections. To comprehend the variations in virulence among distinct strains of GAstV. 12 re...
Source: Frontiers in Microbiology - December 7, 2023 Category: Microbiology Source Type: research
Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research
Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research
Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research
Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research
Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research
Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research
Correction: Rapid detection of goose astrovirus genotypes 2 using real-time reverse transcription recombinase polymerase amplification
(Source: BMC Veterinary Research)
Source: BMC Veterinary Research - November 23, 2023 Category: Veterinary Research Authors: Haiqin Li, Yujun Zhu, Chunhe Wan, Zhangzhang Wang, Lei Liu, Meifang Tan, Fanfan Zhang, Yanbing Zeng, Jiangnan Huang, Chengcheng Wu, Yu Huang, Zhaofeng Kang and Xiaoquan Guo Tags: Correction Source Type: research
Characterisation of human astrovirus in a diarrhoea outbreak using nanopore and Sanger sequencing protocols
In this study, we collect... (Source: Virology Journal)
Source: Virology Journal - November 14, 2023 Category: Virology Authors: Jinhui Li, Lang Yang, Kaiying Wang, Zhiyong Gao, Peihan Li, Yanfeng Lin, Leili Jia, Quanyi Wang, Hongbin Song and Peng Li Tags: Research Source Type: research
Diversity of classic and novel human astrovirus in outpatient children with acute gastroenteritis in Shanghai, China
This study was aimed at investigating the diversity and epidemiology of classic and novel HAstV in outpatient children aged 0–16 years old with AGE in Shanghai.MethodsFrom May 2020 to December 2022, a total of 1,482 stool samples were collected from children diagnosed as AGE from the Children’s Hospital of Fudan University. HAstV was identified using pan-astrovirus consensus primers by Reverse transcription PCR.ResultsDuring the study period, 3.3% (49/1,482) of specimens were identified as HAstV, with a detection rate of 2.5% (37/1,482) for classic HAstV and 0.8% (12/1,482) for novel HAstV. Among the 12 novel HAstV str...
Source: Frontiers in Microbiology - November 13, 2023 Category: Microbiology Source Type: research
Rapid detection of goose astrovirus genotypes 2 using real-time reverse transcription recombinase polymerase amplification
Goose astrovirus (GoAstV) is an important pathogen that causes joint and visceral gout in goslings. It has been circulating in many provinces of China since 2017. Goose astrovirus genotypes 2 (GoAstV-2) is the... (Source: BMC Veterinary Research)
Source: BMC Veterinary Research - November 7, 2023 Category: Veterinary Research Authors: Haiqin Li, Yujun Zhu, Chunhe Wan, Zhangzhang Wang, Lei Liu, Meifang Tan, Fanfan Zhang, Yanbing Zeng, Jiangnan Huang, Chengcheng Wu, Yu Huang, Zhaofeng Kang and Xiaoqiao Guo Tags: Research Source Type: research
Detection and diversity of gastrointestinal viruses in wastewater from Caracas, Venezuela, 2021-2022
Virology. 2023 Oct 23;589:109913. doi: 10.1016/j.virol.2023.109913. Online ahead of print.ABSTRACTGastrointestinal viruses (GIV) are an important cause of childhood morbidity and mortality, particularly in developing countries. Their epidemiological impact in Venezuela during the COVID-19 pandemic remains unclear. GIV can also be detected in domestic sewage. Ninety-one wastewater samples from urban areas of Caracas collected over 12 months and concentrated by polyethylene-glycol-precipitation, were analyzed by multiplex reverse-transcription-PCR for rotavirus/calicivirus/astrovirus and enterovirus/klassevirus/cosavirus, an...
Source: Virology - November 4, 2023 Category: Virology Authors: Alejandra Zamora-Figueroa Rita E Rosales Rixio Fern ández Viviana Ram írez Marjorie Bastardo Alba Far ías Esmeralda Vizzi Source Type: research
Detection and diversity of gastrointestinal viruses in wastewater from Caracas, Venezuela, 2021-2022
Virology. 2023 Oct 23;589:109913. doi: 10.1016/j.virol.2023.109913. Online ahead of print.ABSTRACTGastrointestinal viruses (GIV) are an important cause of childhood morbidity and mortality, particularly in developing countries. Their epidemiological impact in Venezuela during the COVID-19 pandemic remains unclear. GIV can also be detected in domestic sewage. Ninety-one wastewater samples from urban areas of Caracas collected over 12 months and concentrated by polyethylene-glycol-precipitation, were analyzed by multiplex reverse-transcription-PCR for rotavirus/calicivirus/astrovirus and enterovirus/klassevirus/cosavirus, an...
Source: Virology - November 4, 2023 Category: Virology Authors: Alejandra Zamora-Figueroa Rita E Rosales Rixio Fern ández Viviana Ram írez Marjorie Bastardo Alba Far ías Esmeralda Vizzi Source Type: research
The Emergence and Widespread Circulation of Enteric Viruses Throughout the COVID-19 Pandemic: A Wastewater-Based Evidence
This study provides evidence for the emergence and spread of enteric viruses during the pandemic. (Source: Food and Environmental Virology)
Source: Food and Environmental Virology - October 29, 2023 Category: Virology Source Type: research
Viruses, Vol. 15, Pages 2164: Prevalence and Characterization of Gastroenteritis Viruses among Hospitalized Children during a Pilot Rotavirus Vaccine Introduction in Vietnam
h
Parashar
Tate
Trang
Rotavirus (RV), norovirus (NoV), sapovirus (SaV), and human astrovirus (HAstV) are the most common viral causes of gastroenteritis in children worldwide. From 2016 to 2021, we conducted a cross-sectional descriptive study to determine the prevalence of these viruses in hospitalized children under five years old in Nam Dinh and Thua Thien Hue provinces in Vietnam during the pilot introduction of the RV vaccine, Rotavin-M1 (POLYVAC, Hanoi, Vietnam). We randomly selected 2317/6718 (34%) acute diarrheal samples from children <5 years of age enrolled at seven sentinel hospitals from...
Source: Viruses - October 27, 2023 Category: Virology Authors: Mai Ly Doan Oka Mai Quyet Mai Thiem Anh Van Sanh Hien Anh Parashar Tate Trang Tags: Article Source Type: research
