Circulation of unusual and diverse enteric virus strains in adults with acute gastroenteritis: a study from Pune (Maharashtra), Western India
In this study, fecal samples (n = 110) from adults with acute gastroenteritis in Pune, Western India, were tested for six enteric viruses, and the prevalence of these viruses was as follows: rotavirus A (RVA), 38.5%; enterovirus (EV), 23.1%; astrovirus (AstV), 23.1%; adenovirus (AdV), 7.7%; human bocavirus (HBoV), 7.7%; norovirus (NoV), 0%. Circulation of the RVA G1P[8], G3P[8], G9P[4], CVA-10, echovirus E13, EVC-116, AstV-5, AstV-2, HBoV-1, and AdVC-2 types was observed. When compared to the RotaTeq, Rotarix, and RotaVac vaccine strains, antigenic changes were found in the A, B, C, and F regions of the RVA strains. The ci...
Source: Archives of Virology - May 12, 2023 Category: Virology Source Type: research
Rotavirus infections and their genotype distribution in Rwanda before and after the introduction of rotavirus vaccination
by Jean-Claude Kabayiza, Staffan Nilsson, Maria Andersson
Rotavirus vaccination has reduced mortality and hospital admissions due to rotavirus diarrhoea, but its effect on rotavirus infections and the impact of rotavirus genotypes are still unclear. Real-time PCR was used to detect rotavirus and other pathogens in faeces samples from children below five years of age with acute diarrhoea, collected before (n = 827) and after (n = 807, 92% vaccinated) the introduction of vaccination in Rwanda in 2012. Rotavirus was genotyped by targeting VP7 to identify G1, G2, G3, G4, G9 and G12 and VP4 to identify P[4], P[6] and P[8]. In ...
Source: PLoS One - April 25, 2023 Category: Biomedical Science Authors: Jean-Claude Kabayiza Source Type: research
Activated sludge and UV-C254 for Sapovirus, Aichivirus, Astrovirus, and Adenovirus processing
. (Source: International Journal of Environmental Health Research)
Source: International Journal of Environmental Health Research - April 22, 2023 Category: Environmental Health Authors: Chourouk Ibrahim Salah Hammami Nesserine khelifi Pierre Pothier Abdennaceur Hassen Source Type: research
Investigation of removal and inactivation efficiencies of human sapovirus in drinking water treatment processes by applying an in vitro cell-culture system
Water Res. 2023 Apr 7;236:119951. doi: 10.1016/j.watres.2023.119951. Online ahead of print.ABSTRACTHere, we examined the efficiencies of drinking water treatment processes for the removal and inactivation of human sapovirus (HuSaV). We applied a recently developed in vitro cell-culture system to produce purified solutions of HuSaV containing virus concentrations high enough to conduct virus-spiking experiments, to develop an integrated cell culture-polymerase chain reaction (ICC-PCR) assay to quantify the infectivity of HuSaV, and to conduct virus-spiking experiments. In virus-spiking coagulation-sedimentation-rapid sand f...
Source: Cell Research - April 15, 2023 Category: Cytology Authors: D Shirakawa N Shirasaki Q Hu T Matsushita Y Matsui H Takagi T Oka Source Type: research
Genotype Diversity of Enteric Viruses in Wastewater Amid the COVID-19 Pandemic
This study crucially presents the molecular characteristics of circulating AGE viruses, explaining the importance of SW investigation duri ng the pandemic when a clinical investigation may not produce the complete scenario. (Source: Food and Environmental Virology)
Source: Food and Environmental Virology - April 14, 2023 Category: Virology Source Type: research
Rapid diagnosis of duck Tembusu virus and goose astrovirus with TaqMan-based duplex real-time PCR
In this study, by analyzing the published genomes of DTMUV and goose astrovirus genotype 2 (GoAstV-2) isolated in China, we found that both viruses have high conservation, showing 96.5 to 99.5% identities within different strains of DTMUV and GoAstV, respectively. Subsequently, PCR primers and TaqMan probes were designed to identify DTMUV and GoAstV-2, and different fluorescent reporters were given to two probes for differential diagnosis. Through the optimization and verification, this study finally developed a duplex TaqMan qPCR method that can simultaneously detect the above two viruses. The lower limits of detection we...
Source: Frontiers in Microbiology - March 30, 2023 Category: Microbiology Source Type: research
Chicken Astro virus (CAstV): Isolation and characterization of new strains in broiler flocks with poor performance
Abstract
Chicken astroviruses (CAstV) were associated with retarded growth, enteritis, kidney diseases, and white chick syndrome. In the current study, we aimed to evaluate the effect of CAstV infection on growth, performance, and gross and histopathological picture of commercial chicken flocks suffering increased culling rate and decreased performance. Samples were collected for virus isolation, identification, and sequencing on day one, 15 days, and 30 days of age. Body weight, feed conversion rate, and mortality rates were determined. A gross examination was performed, and tissue samples from the liver, intestine,...
Source: Veterinary Research Communications - March 28, 2023 Category: Veterinary Research Source Type: research
Detection and genetic characterization of canine kobuvirus from stray dogs in Shanghai, China
In this study, rectal samples collected from 60 stray dogs in dog shelters were screened for canine kobuvirus and other enteroviruses by quantitative real-time reverse transcription polymerase chain reaction. Canine kobuvirus was detected in 25% (15/60) of the samples. In the 15 positive samples, the coinfection rates of canine distemper virus, canine coronavirus, canine astrovirus, canine norovirus, and canine rotavirus were 26.67%, 20.00%, 73.33%, 0%, and 20.00%, respectively. Phylogenetic analysis based on partial VP1 sequences identified a novel canine kobuvirus that was a recombinant of canine and feline kobuvirus. Ba...
Source: Archives of Virology - March 14, 2023 Category: Virology Source Type: research
Viruses, Vol. 15, Pages 750: First Discovery of Phenuiviruses within Diverse RNA Viromes of Asiatic Toad (Bufo gargarizans) by Metagenomics Sequencing
n Xu
William J. Liu
Most zoonotic pathogens originate from mammals and avians, but viral diversity and related biosafety risk assessment in lower vertebrates also need to be explored. Amphibians are an important group of lower vertebrates that played a momentous role in animal evolution. To elucidate the diversity of RNA viruses in one important species of amphibians, the Asiatic toad (Bufo gargarizans), we obtained 44 samples including lung, gut, liver, and kidney tissues from Asiatic toads in Sichuan and Jilin provinces, China, for viral metagenomics sequencing. More than 20 novel RNA viruses derived from the order...
Source: Viruses - March 14, 2023 Category: Virology Authors: Zhangfu Chen Haiyu Zhao Zhongkuan Li Mengkun Huang Nan Si Hui Zhao Xiaolu Wei Bo Sun George F. Gao Ziqian Xu William J. Liu Tags: Article Source Type: research
Seasonal variation of diarrhoeal pathogens among Guinea-Bissauan children under five years of age
ConclusionIn childhood diarrhoea in a West African LIC, seasonal variation appears to favour EAEC, ETEC, andCryptosporidium in the rainy and viral pathogens in the dry season. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - March 13, 2023 Category: Tropical Medicine Authors: Sointu Mero Source Type: research
Prevalence of sapovirus and astrovirus in pediatric infectious gastroenteritis surveillance in Kobe City, Japan, during 2016-2019
This study indicates that SaV and AstV are important causative viruses of pediatric infectious gastroenteritis.PMID:36858597 | DOI:10.7883/yoken.JJID.2023.037 (Source: Japanese Journal of Infectious Diseases)
Source: Japanese Journal of Infectious Diseases - March 1, 2023 Category: Infectious Diseases Authors: Takeshi Hanafusa Kentaro Arikawa Yoshihiko Tanimoto Source Type: research
