Core antigenic advantage domain-based ELISA to detect antibody against novel goose astrovirus in breeding geese

In conclusion, the developed indirect ELISA is simple, specific, and sensitive, which will be greatly useful to screen GAstV infection and block vertical transmission. KEY POINTS: • B-cell epitopes of GAstV capsid protein were predicted and expressed as immunogen • A core antigenic advantage domain-based ELISA was established to detect GAstV-specific antibody • The established ELISA will contribute to inspection and elimination of infected breeding geese and provide a useful tool for large scale serological testing of GAstV in geese.PMID:35254499 | DOI:10.1007/s00253-022-11852-y

Source: Applied Microbiology and Biotechnology - March 7, 2022 Category: Microbiology Authors: Zeng Wang Huayuan Chen Shenyan Gao Mingzhen Song Zicong Shi Zhifeng Peng Qianyue Jin Li Zhao Hongxing Qiao Chuanzhou Bian Xia Yang Xiaozhan Zhang Jun Zhao Source Type: research