Development of a duplex TaqMan real-time RT-PCR assay for simultaneous detection of goose astrovirus genotypes 1 and 2
J Virol Methods. 2022 May 13:114542. doi: 10.1016/j.jviromet.2022.114542. Online ahead of print.ABSTRACTGoose astrovirus (GAstV) is a novel pathogen that was discovered in 2018. It has two genotypes, GAstV-1 and GAstV-2, and both can cause visceral gout of goslings and result in significant economic losses. The present work aimed to develop a duplex TaqMan real-time quantitative reverse transcription PCR (RT-qPCR) assay to distinguish the two genotypes. MegAlign software was used to design two pairs of primers and a pair of matched probes based on the open reading frame 2 (ORF2) sequence with the greatest difference betwee...
Source: Journal of Virological Methods - May 16, 2022 Category: Virology Authors: Zewen Yi Rui Ding Ruibin Cao Weidong Sun Mingzhi Sun Yongyi Dong Buriro Rehana Endong Bao Yingjun Lv Source Type: research
Development of a duplex TaqMan real-time RT-PCR assay for simultaneous detection of goose astrovirus genotypes 1 and 2
J Virol Methods. 2022 May 13:114542. doi: 10.1016/j.jviromet.2022.114542. Online ahead of print.ABSTRACTGoose astrovirus (GAstV) is a novel pathogen that was discovered in 2018. It has two genotypes, GAstV-1 and GAstV-2, and both can cause visceral gout of goslings and result in significant economic losses. The present work aimed to develop a duplex TaqMan real-time quantitative reverse transcription PCR (RT-qPCR) assay to distinguish the two genotypes. MegAlign software was used to design two pairs of primers and a pair of matched probes based on the open reading frame 2 (ORF2) sequence with the greatest difference betwee...
Source: Journal of Virological Methods - May 16, 2022 Category: Virology Authors: Zewen Yi Rui Ding Ruibin Cao Weidong Sun Mingzhi Sun Yongyi Dong Buriro Rehana Endong Bao Yingjun Lv Source Type: research
Identification of a Novel Astrovirus in Pinnipeds
Astroviruses infect human and animals and cause diarrhea, fever, and vomiting. In severe cases, these infections may be fatal in infants and juvenile animals. Previous evidence showed that humans in contact with infected animals can develop serological responses to astroviruses. Mamastrovirus 11 is a species of Mamastrovirus and was first reported in 2018. It was detected in the fecal samples of a California sea lion. The genome sequence of its capsid protein (CP) was submitted to GenBank. However, the genome sequence of its non-structural protein region was not elucidated. In the present study, we characterized the genome...
Source: Frontiers in Microbiology - May 4, 2022 Category: Microbiology Source Type: research
Determination and Genotype Distribution of Rotavirus Gastroenteritis in Pediatric Patients Admitted to a Tertiary Care Hospital
Mikrobiyol Bul. 2022 Apr;56(2):304-314. doi: 10.5578/mb.20229809.ABSTRACTAcute infectious gastroenteritis is a prevalent disease worldwide, and most of the cases are caused by viral pathogens. Many different viruses, including Rotavirus (RV), Norovirus, Adenovirus, and Astroviruses, are responsible for most acute viral gastroenteritis cases. According to the Centers for Disease Control and Prevention (CDC), viral gastroenteritis infections cause more than 200 000 child mortalities each year worldwide. One of the best strategies to reduce the global burden of RV gastroenteritis is the development and administration of effec...
Source: Mikrobiyoloji Bulteni - April 28, 2022 Category: Microbiology Authors: Fatime Habibe Caneri ği Birol Şafak Source Type: research
