Viruses, Vol. 13, Pages 2072: Herpes Simplex Virus 1 and 2 Infections during Differentiation of Human Cortical Neurons
Viruses, Vol. 13, Pages 2072: Herpes Simplex Virus 1 and 2 Infections during Differentiation of Human Cortical Neurons
Viruses doi: 10.3390/v13102072
Authors:
Petra Bergström
Edward Trybala
Charlotta E. Eriksson
Maria Johansson
Tugce Munise Satir
Sibylle Widéhn
Stefanie Fruhwürth
Wojciech Michno
Faisal Hayat Nazir
Jörg Hanrieder
Soren Riis Paludan
Lotta Agholme
Henrik Zetterberg
Tomas Bergström
Herpes simplex virus 1 (HSV-1) and 2 (HSV-2) can infect the central nervous system (CNS) with dire consequences; in children and adults, HSV-1 may cause focal encephalitis, while HSV-2 causes meningitis. In neonates, both viruses can cause severe, disseminated CNS infections with high mortality rates. Here, we differentiated human induced pluripotent stem cells (iPSCs) towards cortical neurons for infection with clinical CNS strains of HSV-1 or HSV-2. Progenies from both viruses were produced at equal quantities in iPSCs, neuroprogenitors and cortical neurons. HSV-1 and HSV-2 decreased viability of neuroprogenitors by 36.0% and 57.6% (p < 0.0001), respectively, 48 h post-infection, while cortical neurons were resilient to infection by both viruses. However, in these functional neurons, both HSV-1 and HSV-2 decreased gene expression of two markers of synaptic activity, CAMK2B and ARC, and affected synaptic activity negatively in multielectrode array experiments. However, unaltered secretion levels of the neurodegeneration markers tau and...
Source: Viruses - Category: Virology Authors: Petra Bergstr öm Edward Trybala Charlotta E. Eriksson Maria Johansson Tugce Munise Satir Sibylle Wid éhn Stefanie Fruhw ürth Wojciech Michno Faisal Hayat Nazir J örg Hanrieder Soren Riis Paludan Lotta Agholme Henrik Zetterberg Tomas Bergstr öm Tags: Article Source Type: research
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