Age- and Organ-Specific Differences in Mitochondrial Bioenergetics in Brown Norway Rats.

Age- and Organ-Specific Differences in Mitochondrial Bioenergetics in Brown Norway Rats. J Aging Res. 2020;2020:7232614 Authors: Pandya JD, Valdez M, Royland JE, MacPhail RC, Sullivan PG, Kodavanti PRS Abstract Mitochondria play a central role in energy homeostasis and act as regulatory checkpoints for downstream metabolic responses and cell senescence processes during an entire life span. Acute or chronic environmental toxicant exposures have shown deleterious organ-specific human health issues at various life stages. Since mitochondria are a prime target for ensuing cellular bioenergetics responses and senescence, it is essential to understand mitochondrial bioenergetic responses in different organs over multiple life stages. Therefore, in the present study, we evaluated mitochondrial bioenergetic parameters in the liver, lung, and heart in four diverse age groups (young: 1 month; adult: 4 months; middle-aged: 12 months; old-aged: 24 month) using male Brown Norway rats as a model of aging (n = 5 sample size/organ/age group) and compared them with our previously published results on brain. Real-time mitochondrial bioenergetic parameters (i.e., State III, State IV, and State V) were measured using the Seahorse Extracellular Flux Analyzer. Additionally, mitochondrial enzyme pyruvate dehydrogenase complex (PDHC), Complex I, Complex II, and Complex IV activities were measured using Synergy HT plate reader. Our results indicated that...
Source: Journal of Aging Research - Category: Geriatrics Tags: J Aging Res Source Type: research