Synthetic apolipoprotein A-I mimetic peptide 4F protects hearts and kidneys after myocardial infarction.

Synthetic apolipoprotein A-I mimetic peptide 4F protects hearts and kidneys after myocardial infarction. Am J Physiol Regul Integr Comp Physiol. 2020 Jan 22;: Authors: Moreira RS, Irigoyen MC, Capcha JMC, Sanches TR, Gutierrez PS, Garnica MR, Noronha IL, Andrade L Abstract Patients undergoing coronary angiography after myocardial infarction (MI) often develop cardiac and renal dysfunction. We hypothesized that the apolipoprotein A-I mimetic peptide 4F (hereafter, 4F) would prevent those complications. Male Wistar rats were fed a high-cholesterol diet for 8 days. The rats were then anesthetized with isoflurane and randomly divided into five groups a control group (sham-operated rats); and four groups of rats induced to MI by left coronary artery ligation, the rats in three of those groups being injected, 6 h later, with the nonionic contrast agent iopamidol, 4F, and iopamidol plus 4F, respectively. At post-procedure hour 24, we performed the following experiments/tests (n = 8 rats/group): metabolic cage studies; creatinine clearance studies; analysis of creatinine, urea, sodium, potassium, triglycerides, total cholesterol, VLDL, LDL, and HDL; immunohistochemistry; histomorphometry; Western blotting; and transmission electron microscopy. In another set of experiments (n = 8 rats/group), also performed at post-procedure hour 24, we measured mean arterial pressure, heart rate, heart rate variability, echocardiographic parameters, left ve...
Source: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology - Category: Physiology Authors: Tags: Am J Physiol Regul Integr Comp Physiol Source Type: research