Cancers, Vol. 12, Pages 226: Human Medulloblastoma Cell Lines: Investigating on Cancer Stem Cell-Like Phenotype
Cancers, Vol. 12, Pages 226: Human Medulloblastoma Cell Lines: Investigating on Cancer Stem Cell-Like Phenotype
Cancers doi: 10.3390/cancers12010226
Authors:
Arianna Casciati
Mirella Tanori
Rémi Manczak
Sofiane Saada
Barbara Tanno
Paola Giardullo
Elena Porcù
Elena Rampazzo
Luca Persano
Giampietro Viola
Claire Dalmay
Fabrice Lalloué
Arnaud Pothier
Caterina Merla
Mariateresa Mancuso
Medulloblastoma (MB) is the most common malignant pediatric brain tumor. Despite the progress of new treatments, the risk of recurrence, morbidity, and death remains significant and the long-term adverse effects in survivors are substantial. The fraction of cancer stem-like cells (CSCs) because of their self-renewal ability and multi-lineage differentiation potential is critical for tumor initiation, growth, and resistance to therapies. For the development of new CSC-targeted therapies, further in-depth studies are needed using enriched and stable MB-CSCs populations. This work, aimed at identifying the amount of CSCs in three available human cell lines (DAOY, D341, and D283), describes different approaches based on the expression of stemness markers. First, we explored potential differences in gene and protein expression patterns of specific stem cell markers. Then, in order to identify and discriminate undifferentiated from differentiated cells, MB cells were characterized using a physical characterization method based on a high-frequency dielectrophoresis ap...
Source: Cancers - Category: Cancer & Oncology Authors: Arianna Casciati Mirella Tanori R émi Manczak Sofiane Saada Barbara Tanno Paola Giardullo Elena Porc ù Elena Rampazzo Luca Persano Giampietro Viola Claire Dalmay Fabrice Lallou é Arnaud Pothier Caterina Merla Mariateresa Mancuso Tags: Article Source Type: research
More News: Brain | Brain Cancers | Brain Tumor | Cancer | Cancer & Oncology | Genetics | Medulloblastoma | Neurology | Pediatrics | Stem Cell Therapy | Stem Cells | Study | Undifferentiated Carcinoma