Acquisition of mcr-1 and co-carriage of virulence genes in avian pathogenic Escherichia coli isolates from municipal wastewater influents in Japan.

In conclusion, mcr-1-positive E. coli environmental isolates were mostly characterised by positive for APEC-associated virulence genes. Co-presence of those genes may suggest the existence of common source in animals and/or their associated environments.IMPORTANCE Colistin is considered as a last-line therapeutic option in severe infections due to multidrug-resistant Gram-negative bacteria, in particular carbapenemase-producing Enterobacteriaceae and multidrug-resistant Acinetobacter baumannii Increasing prevalence of mcr genes in diverse Enterobacteriaceae species, mainly E. coli and Klebsiella pneumoniae from humans and food animals has become a significant concern to public health all over the world. In Japan, mcr genes have so far been detected in food animals, raw meat, wastewater, and human clinical samples. This study reports the co-presence of mcr-1 and APEC-associated virulence genes in five of seven E. coli isolates recovered from aquatic environments in Japan. Our study highlights the importance and urgency of action to reduce environmental contamination by mcr genes that may likely occur due to exposure to untreated wastewater through combined sewer overflow by recent unusual weather. PMID: 31492666 [PubMed - as supplied by publisher]
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research

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This article reports the characterization and evaluation of the biotechnological potential of a cysteine protease purified from Calotropis procera (CpCP3). This enzyme was highly stable to different metal ions and was able to hydrolyze κ-casein similarly to bovine chymosin. Atomic force microscopy showed that the process of casein micelle aggregation induced by CpCP3 was similar to that caused by chymosin. The cheeses made using CpCP3 showed higher moisture content than those made with chymosin, but protein, fat, and ash were similar. The sensory analysis showed that cheeses made with CpCP3 had high acceptance index ...
Source: Food Chemistry - Category: Food Science Source Type: research
Publication date: Available online 13 October 2019Source: Redox BiologyAuthor(s): Kaibo Xie, Marharyta Varatnitskaya, Abdelouahid Maghnouj, Verian Bader, Konstanze F. Winklhofer, Stephan Hahn, Lars I. LeichertAbstractNeutrophils produce a cocktail of oxidative species during the so-called oxidative burst to attack phagocytized bacteria. However, little is known about the neutrophils' redox homeostasis during the oxidative burst and there is currently no consensus about the interplay between oxidative species and cellular signaling, e.g. during the initiation of the production of neutrophil extracellular traps (NETs). Using...
Source: Redox Biology - Category: Biology Source Type: research
Publication date: Available online 13 October 2019Source: Carbohydrate PolymersAuthor(s): Shaolei Kang, Hualin Wang, Li Xia, Minmin Chen, Linlin Li, Junfeng Cheng, Xingjiang Li, Shaotong JiangAbstractIn this work, a colorimetric film was designed for shrimp freshness monitoring by incorporating rose anthocyanins (RAs) in polyvinyl alcohol/okra mucilage polysaccharide (PVA/OMP) composite film. The presence of OMP changed the film-forming solution from Newtonian to non-Newtonian fluid. The addition of OMP and RAs decreased the crystalline of PVA due to the hydrogen bonds among RAs, OMP and PVA. An appropriate content of RAs ...
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research
Publication date: Available online 13 October 2019Source: Sensors and Actuators B: ChemicalAuthor(s): Asya Kalinichenko, Larysa ArseniyevaAbstractThis paper is devoted to the multiclass classification of quartz crystal microbalance sensor-array data based on two chemometric approaches, and aimed at the development of rapid analytical technique (both at the chemical and data levels) for detection and quantitative assessment of soy protein at the levels 0, 10, 20, 30% w/w in sausages for avoiding different type of frauds and thus providing the effective meat products quality control issue. The first approach was based on the...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research
Publication date: Available online 14 October 2019Source: Chemical Engineering ScienceAuthor(s): Xueliang Li, Guoqiang Zhang, Xinrui Zhao, Jingwen Zhou, Guocheng Du, Jian ChenAbstractCell-based meat, or cultured meat is at the early commercialization stage, but thorough considerations of the scaling-up challenges are scarce and the physiological data of animal muscle cells required by reliable process scaling-up are severely lacking. To make cultured meat an economically viable source of animal proteins, we propose a rational approach towards the design of a large scale air-lift reactor for cultured meat manufacturing. Com...
Source: Chemical Engineering Science - Category: Chemistry Source Type: research
In this study, partially substituted analog D-Arg-W3R6 and completely substituted D-enantiomer D-W3R6 were synthesized. The resistance of D-Arg-W3R6 and D-W3R6 to cleavage by the tested protease increased, particularly of D-W3R6. The antimicrobial activity of D-Arg-W3R6 was almost the same as that of the parent peptide W3R6, but the antimicrobial activity of D-W3R6 was slightly decreased. The hemolytic activity of both D-Arg-W3R6 and D-W3R6 was negligible. The CD spectrum of D-W3R6 exhibited symmetry with that of W3R6 in a membrane-mimetic environment. The membrane interaction between the D-amino acid substituted analogs a...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research
More than 50 people are dead in the aftermath of Typhoon Hagibis, which dropped extreme amounts of rain over the weekend and caused catastrophic flooding in residential areas. (Image credit: Jae C. Hong/AP)
Source: NPR Health and Science - Category: Consumer Health News Authors: Source Type: news
Source: BMJ News - Category: General Medicine Source Type: research
By Emily Reynolds With increased concern about the impact of meat on our health and the environment, and an ever-expanding selection of meat-free products available to buy, popular interest in vegetarianism and veganism has steadily grown. But for those who want to cut down but aren’t quite ready to give up their burgers, there is a third way: flexitarianism. As a 2019 study from the University of Nottingham on red meat and heart health put it, you “don’t have to go cold turkey on red meat to see health benefits”, finding that halving the amount of red and processed meat eaten can have signific...
Source: BPS RESEARCH DIGEST - Category: Psychiatry & Psychology Authors: Tags: Eating Social The self Source Type: blogs
[VOA] Rabbit meat is a slowly growing part of Africa's diet, with a burgeoning industry in South Africa and Nigeria, and a group of hardcore rabbit farmers promoting the meat as cheap, healthy and environmentally friendly. But will it take off? Critics say they're reluctant to eat animals that are considered pets.
Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
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