Effects of Tokishakuyakusan on regeneration of murine olfactory neurons in vivo and in vitro.

Effects of Tokishakuyakusan on regeneration of murine olfactory neurons in vivo and in vitro. Chem Senses. 2019 Apr 16;: Authors: Noda T, Shiga H, Yamada K, Harita M, Nakamura Y, Ishikura T, Kumai M, Kawakami Z, Kaneko A, Hatta T, Sakata-Haga H, Shimada H, Miwa T Abstract Post-upper respiratory tract infection related olfactory dysfunction typically occurs due to neural damage after an upper respiratory tract infection associated with a common cold or influenza. Recently, Tokishakuyakusan, a Japanese traditional Kampo medicine, has been found to be effective for post-viral olfactory dysfunction. However, the pharmacodynamics of Tokishakuyakusan in the treatment of post-viral olfactory dysfunction remain unresolved. We investigated the effects of Tokishakuyakusan on the regeneration of olfactory neurons and expression of nerve growth factor (NGF) in neural systems, using in vivo murine studies and in vitro cell culture studies. Eight-week-old BALB/C female mice were fed a pellet diet with or without Tokishakuyakusan. Degeneration of cells in olfactory epithelium was induced by intraperitoneal methimazole injection. Regeneration of olfactory neurons was observed by histological and immunohistochemical procedures. NGF expression in the olfactory bulb was measured by enzyme-linked immune-assay. NGF gene and protein expression was measured using rat primary cultured astrocytes by real-time polymerase chain reaction and enzyme-linked immun...
Source: Chemical Senses - Category: Biochemistry Authors: Tags: Chem Senses Source Type: research