DNA methylation and expression status of glutamate receptor genes in patients with oral squamous cell carcinoma

Publication date: Available online 16 January 2019Source: Meta GeneAuthor(s): Mohammad AyoubRigi-Ladiz, Tayebeh Baranzehi, Behnaz Hassanpour, Mohammad Javad Ashraf, Dor Mohammad Kordi-TamandaniAbstractBackgroundOral cancer represents the third most prevalent form of malignancy in developing countries and the eight most common form of cancer in developed countries. Alcohol and tobacco users are most affected by oral cancers and 90% of them are OSCC in adult males. In some provinces in Iran such as Sistan and Baluchestan, its prevalence is higher compared to other provinces. One of the primary neurotransmitter in the central nervous system is systemic glutamate, which is a major excitatory neurotransmitter. Glutamate signaling has been involved in various non-neuronal cancer processes. The aim of this research was to highlight the association between DNA methylation of the glutamate receptor genes and their expression pattern in pathogenesis of OSCC.Materials and methodsGenomic DNA was isolated from 83 OSCC paraffin-embedded tissues (mean age: 59.67 ± 16.08) and 80 normal samples (mean age: 50.15 ± 16.69). Promoter methylation status of glutamate receptors including GRM5, GRM2 and GRIA3 genes were carried out by Methylation Specific PCR technique (MSP). We also investigated the mRNA expression levels of these genes in 15 paraffin-embedded patients and healthy samples using real-time PCR techniques.ResultDNA methylation analysis showed statistically significant differe...
Source: Meta Gene - Category: Genetics & Stem Cells Source Type: research