Antinociceptive activity of ethanolic extract of Azadirachta indica A. Juss (Neem, Meliaceae) fruit through opioid, glutamatergic and acid-sensitive ion pathways in adult zebrafish (Danio rerio)

Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Francisco Lucas A. Batista, Luiza M.G. Lima, Izamar A. Abrante, José Ismael F. de Araújo, Francisca Leidivania A. Batista, Izabel A. Abrante, Erlândia A. Magalhães, Daniele R. de Lima, Maria da Conceição L. Lima, Brenda Silva do Prado, Luiz F.W.G. Moura, Maria Izabel F. Guedes, Maria Kueirislene A. Ferreira, Jane Eire Silva Alencar de Menezes, Sacha Aubrey A.R. Santos, Francisco Rogênio S. Mendes, Renato A. Moreira, Ana Cristina O. Monteiro-Moreira, Adriana R. Campos, Francisco Ernani A. MagalhãesAbstractNeem fruit (Azadirachta indica A. Juss.) are popularly used to treat infections, diarrhea, fever, bronchitis, skin diseases, infected burns and hypertension. Although the antinociceptive and anti-inflammatory potential of A. indica has already been investigated in experimental models of pain and inflammation in mice, the current research is the first to report the evaluation of the capacity of A. indica fruit ethanolic extract (EtFrNeem) in acute pain attenuation using the adult zebrafish (Danio rerio) as an alternative model to the use in rodents. EtFrNeem was submitted to antioxidant action, preliminary chemical prospecting, FT-IR and determination of phenol and flavonoid content tests. Subsequently, EtFrNeem was tested for acute nociception and abdominal inflammation, locomotor activity, and acute toxicity in adult zebrafish. Possible neuromodulation mechanisms were also eval...
Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research