Culturing Cells in Defined 3-D Structures

In this study, the authors present in-air microfluidics (IAMF), a new chip-free platform technology that enables in-flight (that is, on-the-fly) formation of droplets, fibers, and particles and their one-step deposition into 3D constructs with a modular internal architecture.Figure: Concept of IAMF and guide to the article. (A) Chip-based microfluidics enables in-line control over droplets and particles, making it a versatile platform technology. A chip design where droplets (blue) are transported by a coflow (pink) is shown. (B) IAMF maintains the in-line control of chip-based microfluidics but relies on jet ejection and coalescence into air. Therefore, a wide range of droplets and particles can be produced at flow rates typically two orders of magnitude higher than with chip-based microfluidics. When combining reactive, solidifying microjets, IAMF also enables on-the-fly production and direct deposition of microparticles into 3D multiscale modular (bio)materials.Figure: One-step additive manufacturing and injection molding of 3D multiscale modular (bio)materials. (A) Modular free forms with a controlled microarchitecture were manufactured by stacking of shape-stable core-shell particles. (B to D) A hollow cylinder was formed by deposition of the composite jet onto a rotating substrate. By altering the building blocks ’ composition, the resulting microarchitecture consisted of (C) a liquid-filled foam or (D) a multimaterial modular solid, where the cross-linker for the cor...
Source: Neuromics - Category: Neuroscience Tags: 3-D Bioprinting 3-D Cell based Assays Bio-inks Fibroblast Growth Factor Basic Human Mesenchymal Stem Cells ISOKine FGF Source Type: news