Sildenafil citrate ameliorates the adverse effects of cryopreservation on sperm quality in asthenozoospermic men
In this study, the effects of Sildenafil Citrate on the sperm quality during cryopreservation in the asthenozoospermic patients were investigated for the first time. Thirty semen samples were collected from asthenozoospermic patients and each sample was divided into 3 groups: Control (fresh), Freeze and Freeze + Sildenafil. In each groups the sperm parameters, DNA fragmentation, acrosome integrity, protamine deficiency, mitochondrial membrane potential, plasma membrane integrity, the expression of Bcl-2 and HSP70 genes, as well as the level of Tumor necrosis factor-alpha, Malondialdehyde and antioxidants (Catalase, Glutath...
Source: Cryobiology - May 19, 2023 Category: Biology Authors: Ronak Kohzadi Ebrahim Cheraghi Malek Soleimani Mehranjani MohammadAli Shariatzadeh Source Type: research
Sildenafil citrate ameliorates the adverse effects of cryopreservation on sperm quality in asthenozoospermic men
In this study, the effects of Sildenafil Citrate on the sperm quality during cryopreservation in the asthenozoospermic patients were investigated for the first time. Thirty semen samples were collected from asthenozoospermic patients and each sample was divided into 3 groups: Control (fresh), Freeze and Freeze + Sildenafil. In each groups the sperm parameters, DNA fragmentation, acrosome integrity, protamine deficiency, mitochondrial membrane potential, plasma membrane integrity, the expression of Bcl-2 and HSP70 genes, as well as the level of Tumor necrosis factor-alpha, Malondialdehyde and antioxidants (Catalase, Glutath...
Source: Cryobiology - May 19, 2023 Category: Biology Authors: Ronak Kohzadi Ebrahim Cheraghi Malek Soleimani Mehranjani MohammadAli Shariatzadeh Source Type: research
Molecules, Vol. 28, Pages 4116: Determination of Phosphodiesterase Type-5 Inhibitors (PDE-5) in Dietary Supplements
This study proposed a high-performance thin-layer chromatography (HPTLC) screening method to detect phosphodiesterase 5 (PDE-5) inhibitors as possible adulterant agents in various dietary supplements. Chromatographic analysis was performed on silica gel 60F254 plates using a mixture of ethyl acetate:toluene:methanol:ammonia in a volume ratio of 50:30:20:0.5 as a mobile phase. The system provided compact spots and symmetrical peaks of sildenafil and tadalafil with retardation factor values of 0.55 and 0.90, respectively. The analysis of products purchased from the internet or specialized stores demonstrated the presence of ...
Source: Molecules - May 16, 2023 Category: Chemistry Authors: Oana Ramona C ătălina Gheorghiu Anne Marie Ciobanu Claudia Maria Gu țu Carmen Lidia Chi țescu Giorgiana Valentina Costea Daniela M ădălina Anghel Ana Maria Vlasceanu Daniela Luiza Baconi Tags: Article Source Type: research
Viruses, Vol. 15, Pages 1157: Efficacy of Sildenafil in Patients with Severe COVID-19 and Pulmonary Arterial Hypertension
This study aimed to investigate the clinical efficacy of sildenafil in patients with severe COVID-19 and PAH. Intensive care unit (ICU) patients were randomly assigned to receive sildenafil or a placebo, with 75 participants in each group. Sildenafil was administered orally at 0.25 mg/kg t.i.d. for one week in a placebo-controlled, double-blind manner as an add-on therapy alongside the patient’s routine treatment. The primary endpoint was one-week mortality, and the secondary endpoints were the one-week intubation rate and duration of ICU stay. The mortality rate was 4% vs. 13.3% (p = 0.078), the intubation r...
Source: Viruses - May 11, 2023 Category: Virology Authors: Oleksandr Valentynovych Oliynyk Marta Rorat Olena Vadymivna Strepetova Serhij Oleksandrovych Dubrov Vitaliy Grygorovych Guryanov Yanina Volodymyrivna Oliynyk Oleksii Serhijovych Kulivets Anna Ślifirczyk Wojciech Barg Tags: Article Source Type: research
Study UNICO: Perception of Urologists and Andrologists, in Spain, about the Use of Sildenafil Oral Suspension in Patients with Erectile Dysfunction
CONCLUSIONS: Urologists and andrologists consider that most patients with ED achieve a high degree of satisfaction with sildenafil oral suspension. The main advantage of the treatment is the possibility of adjusting the dose according to patient's needs and circumstances.PMID:37139619 | DOI:10.56434/j.arch.esp.urol.20237602.15 (Source: Archivos Espanoles de Urologia)
Source: Archivos Espanoles de Urologia - May 4, 2023 Category: Urology & Nephrology Authors: Fran çois Peinado Ibarra Borja Garc ía Gómez Saturnino Luj án Marco Ana Mar ía Segura Paños Source Type: research
