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Total 76 results found since Jan 2013.

Long Non-coding RNA-mRNA Correlation Analysis Reveals the Potential Role of HOTAIR in Pathogenesis of Sporadic Thoracic Aortic Aneurysm.
CONCLUSION: Transcriptomic and lncRNA-mRNA correlation analysis revealed HOTAIR was downregulated in STAA and associated with genes involved in extracellular matrix remodelling. In vitro experiments confirmed that knockdown of HOTAIR could induce apoptosis and suppress collagen types I and III expression in human aortic smooth muscle cells. PMID: 28757056 [PubMed - as supplied by publisher]
Source: PubMed: Eur J Vasc Endovasc ... - July 27, 2017 Category: Surgery Authors: Guo X, Chang Q, Pei H, Sun X, Qian X, Tian C, Lin H Tags: Eur J Vasc Endovasc Surg Source Type: research

Silencing the livin gene enhances the cytotoxic effects of anticancer drugs on colon cancer cells.
CONCLUSION: siRNA-mediated down-regulation of livin gene expression could significantly suppress colon cancer growth and enhance the cytotoxic effects of anticancer drugs such as 5-FU and L-OHP. The results of this study suggest that silencing livin gene expression in combination with treatment with anticancer drugs might be a novel cancer therapy for colorectal cancer. PMID: 27904848 [PubMed - in process]
Source: Annals of Surgical Treatment and Research - December 3, 2016 Category: Surgery Tags: Ann Surg Treat Res Source Type: research

Inhibitory Effect of TLR4 Gene Silencing on Intimal Hyperplasia of Vein Grafting
Conclusion: These results showed that local silencing of TLR4 in the vein grafts could inhibit intimal hyperplasia by downregulating the expression of inflammatory factors in the vein grafts, suggesting that TLR4 can be used as a new target for therapy of vascular intimal hyperplasia.
Source: Vascular and Endovascular Surgery - October 11, 2016 Category: Surgery Authors: Zhu, Z., Xu, R., Zheng, X., Wang, T., Li, D., Wang, Y., Liu, K. Tags: Original Manuscripts Source Type: research

Development of a Hybrid Cryogel-Coated Prosthetic Vascular Graft for Delivery of Targeted Gene Therapies
Anastomotic neointimal hyperplasia remains a limiting factor in the long-term success of prosthetic vascular grafts. Understanding of the molecular mechanisms involved in the host response to prosthetic material has set the stage for using this material to deliver small interfering RNA (siRNA) and other modulators of the anastomotic neointimal hyperplasia-associated transcriptome response, along with antithrombotic agents. To create practical and effective drug delivery from a prosthetic material, we have combined a platform of polyethylene terephthalate (PET) fabric with an applied cryogel carrying the biologic agents, re...
Source: Journal of Vascular Surgery - August 23, 2016 Category: Surgery Authors: Cindy Huynh, Ting-Yu Shih, Amruta Samant, Saif G. Pathan, David W. Nelson, David J. Mooney, Leena Pradhan-Nabzdyk, Frank W. LoGerfo Tags: Abstract from the 2016 Eastern Vascular Society Annual Meeting Source Type: research

Heat shock protein A12B protects against sepsis-induced impairment in vascular endothelial permeability.
CONCLUSIONS: HSPA12B plays a protective role in vascular endothelial barrier dysfunction by preserving the endothelial permeability during sepsis. PMID: 27083952 [PubMed - in process]
Source: Hand Surgery - April 19, 2016 Category: Surgery Authors: Kang Q, Chen Y, Zhang X, Yu G, Wan X, Wang J, Bo L, Zhu K Tags: J Surg Res Source Type: research

Inhibition of cyclooxygenase‐1 and ‐2 activity in keratinocytes inhibits PGE2 formation and impairs vascular endothelial growth factor release and neovascularisation in skin wounds
This study investigated the role of Cox activity in the regulation of vascular endothelial growth factor (VEGF) expression in keratinocytes and the formation of new blood vessels in acute wounds in mice. To this end, human HaCaT keratinocytes were stimulated with epidermal growth factor (EGF). EGF increased Cox‐1 mRNA in the presence of the constitutively expressed Cox‐1 protein in keratinocytes. EGF coinduced Cox‐2 and VEGF165 mRNA and protein expression and an accumulation of prostaglandin E2 (PGE2) in cell culture supernatants. Inhibition of Cox isozyme activity by Cox‐1 and ‐2 siRNA or ibuprofen reduced PGE2 ...
Source: International Wound Journal - December 17, 2015 Category: Surgery Authors: Itamar Goren, Seo‐Youn Lee, Damian Maucher, Rolf Nüsing, Thomas Schlich, Josef Pfeilschifter, Stefan Frank Tags: ORIGINAL ARTICLE Source Type: research

Inhibition of cyclooxygenase ‐1 and ‐2 activity in keratinocytes inhibits PGE2 formation and impairs vascular endothelial growth factor release and neovascularisation in skin wounds
This study investigated the role of Cox activity in the regulation of vascular endothelial growth factor (VEGF) expression in keratinocytes and the formation of new blood vessels in acute wounds in mice. To this end, human HaCaT keratinocytes were stimulated with epidermal growth factor (EGF). EGF increased Cox‐1 mRNA in the presence of the constitutively expressed Cox‐1 protein in keratinocytes. EGF coinduced Cox‐2 and VEGF165 mRNA and protein expression and an accumulation of prostaglandin E2 (PGE2) in cell culture supernatants. Inhibition of Cox isozyme activity by Cox‐1 and ‐2 siRNA or ibuprofen reduced PGE2 ...
Source: International Wound Journal - December 16, 2015 Category: Surgery Authors: Itamar Goren, Seo ‐Youn Lee, Damian Maucher, Rolf Nüsing, Thomas Schlich, Josef Pfeilschifter, Stefan Frank Tags: ORIGINAL ARTICLE Source Type: research

Therapeutic Potential and Pitfalls of Gene Silencing in the Arterial Wall
Intimal hyperplasia (IH) is a limitation in effectiveness of surgical and endovascular procedures. This is a study of delivery of siRNA to the arterial wall under clinically applicable conditions to achieve gene silencing. Previously we had identified increased expression of Thrombospondin-2 (TSP-2) as a high profile target associated with IH.
Source: Journal of the American College of Surgeons - September 14, 2015 Category: Surgery Authors: Thomas C.F. Bodewes, Joel M. Johnson, Mauricio A. Contreras, Leena Pradhan-Nabzdyk, Frank W. LoGerfo Tags: Vascular Surgery Source Type: research

Comparative Analysis of Polymers for siRNA Delivery in Vascular Smooth Muscle Cells
The use of short interfering RNA (siRNA) to degrade mRNA in the cell cytoplasm and transiently attenuate intracellular proteins shows promise in the inhibition of vascular pathogenesis. However, a critical obstacle for therapeutic application is a safe and effective delivery system. Biodegradable polymers are promising alternative molecular carriers for genetic material. Here we aim to perform a comparative analysis of poly(B-amino ester) (PBAE) and polyethylenimine (PEI) polymers in their efficacy for vascular smooth muscle cell (VSMC) transfection using siRNA against the GAPDH housekeeping gene as our test target.
Source: Journal of Surgical Research - July 17, 2015 Category: Surgery Authors: Lindsay M. Bools, Richard K. Fisher, Oscar H. Grandas, Stacy S. Kirkpatrick, Joshua D. Arnold, Mitchell H. Goldman, Michael B. Freeman, Deidra J.H. Mountain Source Type: research

Comparative analysis of polymers for short interfering RNA delivery in vascular smooth muscle cells
The use of short interfering RNA (siRNA) to degrade messenger RNA in the cell cytoplasm and transiently attenuate intracellular proteins shows promise in the inhibition of vascular pathogenesis. However, a critical obstacle for therapeutic application is a safe and effective delivery system. Biodegradable polymers are promising alternative molecular carriers for genetic material. Here, we aim to perform a comparative analysis of poly(B-amino ester) (PBAE) and polyethylenimine (PEI) polymers in their efficacy for vascular smooth muscle cell transfection using siRNA against the glyceraldehyde 3-phosphate dehydrogenase (GAPDH...
Source: Journal of Surgical Research - July 17, 2015 Category: Surgery Authors: Lindsay M. Bools, Richard K. Fisher, Oscar H. Grandas, Stacy S. Kirkpatrick, Joshua D. Arnold, Mitchell H. Goldman, Michael B. Freeman, Deidra J.H. Mountain Source Type: research

Nucleolin Enhances the Proliferation and Migration of Heat‐denatured Human Dermal Fibroblasts
This article is protected by copyright. All rights reserved.
Source: Wound Repair and Regeneration - March 1, 2015 Category: Surgery Authors: Bimei Jiang, Yuanbin Li, Pengfei Liang, Yanjuan Liu, Xu Huang, Zhongyi Tong, Pihong Zhang, Xiaoyuan Huang, Ying Liu, Zhenguo Liu Tags: Original Research–Basic Science Source Type: research

p120‐catenin modulating nuclear factor‐κB activation is partially RhoA/ROCK dependent in scratch injury
This article is protected by copyright. All rights reserved.
Source: Wound Repair and Regeneration - February 18, 2015 Category: Surgery Authors: Shenghui Qin, Lingzhi Qin, Chao Zhang, Liwei Liu, Wenjia Sun, Naping Li, Renliang Wu, Xi Wang Tags: Research Article Source Type: research

Acellular dermal matrix-based gene therapy augments graft incorporation
Acellular dermal matrix (ADM) is widely used for structural or dermal replacement purposes. Given its innate biocompatibility and its potential to vascularize, we explored the possibility of ADM to function as a small interfering RNA (siRNA) delivery system. Specifically, we sought to improve ADM vascularization by siRNA-mediated inhibition of prolyl hydroxylase domain-2 (PHD2), a cytoplasmic protein that regulates hypoxia inducible factor-1α, and improve neovascularization.
Source: Journal of Surgical Research - January 30, 2015 Category: Surgery Authors: Meredith T. Vandegrift, Caroline Spzalski, Denis Knobel, Andrew Weinstein, Maria Ham, Obinna Ezeamuzie, Stephen M. Warren, Pierre B. Saadeh Source Type: research

Normalizing Dysfunctional Purine Metabolism Accelerates Diabetic Wound Healing
This study evaluates the role of XO in the diabetic wound and the impact of specifically inhibiting its activity on wound healing. Treatment of diabetic wounds with siXDH (xanthine dehydrogenase siRNA) decreased XDH mRNA expression by 51.6%, XO activity by 35.9%, ROS levels by 78.1%, and pathologic wound burden by 31.5%, and accelerated wound healing by 7 days (23.3%). PCR analysis demonstrated that increased XO activity in wild type wound may be due to XDH to XO conversion and/or XO phosphorylation, but not to gene transcription, whereas increased XO activity in diabetic wounds may also be from gene transcription. These r...
Source: Wound Repair and Regeneration - January 9, 2015 Category: Surgery Authors: Andrew L. Weinstein, Frank D. Lalezarzadeh, Marc A. Soares, Pierre B. Saadeh, Daniel J. Ceradini Tags: Young Investigator Award Source Type: research

Induction of chemokine (C-C motif) ligand 2 by sphingosine-1-phosphate signaling in neuroblastoma
Conclusions: Taken together, our data for the first time demonstrate that S1P induced the macrophage-recruiting factor CCL2 expression in NB cells via S1P2, providing new insights into the complicated functions of S1P2 in cancer.
Source: Journal of Pediatric Surgery - May 19, 2014 Category: Surgery Authors: Mei-Hong Li, Miriam Harel, Timothy Hla, Fernando Ferrer Tags: Original Articles Source Type: research