Comparative analysis of polymers for short interfering RNA delivery in vascular smooth muscle cells

The use of short interfering RNA (siRNA) to degrade messenger RNA in the cell cytoplasm and transiently attenuate intracellular proteins shows promise in the inhibition of vascular pathogenesis. However, a critical obstacle for therapeutic application is a safe and effective delivery system. Biodegradable polymers are promising alternative molecular carriers for genetic material. Here, we aim to perform a comparative analysis of poly(B-amino ester) (PBAE) and polyethylenimine (PEI) polymers in their efficacy for vascular smooth muscle cell transfection using siRNA against the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) housekeeping gene as our test target.

Source: Journal of Surgical Research - July 17, 2015 Category: Surgery Authors: Lindsay M. Bools, Richard K. Fisher, Oscar H. Grandas, Stacy S. Kirkpatrick, Joshua D. Arnold, Mitchell H. Goldman, Michael B. Freeman, Deidra J.H. Mountain Source Type: research