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Specialty: Laboratory Medicine
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Total 432 results found since Jan 2013.
Akt plays indispensable roles during the first cell lineage differentiation of mouse
AbstractThe first cell lineage differentiation occurs during the development of mouse 8-cell embryo to blastocyst. Akt is a potent kinase whose role during blastocyst formation has not been elucidated. In the present study, immunofluorescence results showed that the Akt protein was specifically localized to the outer cells of the morula. Akt-specific inhibitor MK2206 significantly inhibited mouse blastocyst formation and resulted in decreased expression of the trophectoderm marker Cdx2 and led to granular distribution of ER α in the cytoplasm. Furthermore, knockdown of ERα by siRNA microinjection can also lead to a decre...
Source: Journal of Molecular Histology - June 12, 2019 Category: Laboratory Medicine Source Type: research
MIF/CD74 axis participates in inflammatory activation of Schwann cells following sciatic nerve injury
AbstractBased on deep RNA sequencing of distal segments of lesioned sciatic nerves, a huge number of differentially expression genes (DEGs) were thus obtained and functionally analyzed. The inflammatory response was denoted as one of most significant biological processes following sciatic nerve injury. In the present study, ingenuity pathway analysis (IPA) demonstrated that macrophage migration inhibitory factor (MIF) was identified as a core regulator of inflammatory response through interaction with CD74 membrane receptor. By establishment of rat sciatic nerve transection model, we displayed that MIF was upregulated foll...
Source: Journal of Molecular Histology - June 12, 2019 Category: Laboratory Medicine Source Type: research
An Oncogenic Role for Four-Jointed Box 1 (FJX1) in Nasopharyngeal Carcinoma.
Authors: Chai SJ, Ahmad Zabidi MM, Gan SP, Rajadurai P, Lim PVH, Ng CC, Yap LF, Teo SH, Lim KP, Patel V, Cheong SC
Abstract
Nasopharyngeal carcinoma (NPC) is a highly metastatic cancer prevalent in Southern China and Southeast Asia. The current knowledge on the molecular pathogenesis of NPC is still inadequate to improve disease management. Using gene expression microarrays, we have identified the four-jointed box 1 (FJX1) gene to be upregulated in primary NPC tissues relative to nonmalignant tissues. An orthologue of human FJX1, the four-jointed (fj) gene in Drosophila and Fjx1 in mouse, has reported to be associa...
Source: Disease Markers - June 28, 2019 Category: Laboratory Medicine Tags: Dis Markers Source Type: research
Binding to carboxypeptidase M mediates protective effects of fibrinopeptide B β15-42.
Binding to carboxypeptidase M mediates protective effects of fibrinopeptide Bβ15-42.
Transl Res. 2019 Jul 26;:
Authors: Sörensen-Zender I, Chen R, Song R, David S, Melk A, Haller H, Schmitt R
Abstract
During fibrinolysis a 28-amino-acid peptide is generated besides other degradation products of fibrin. This peptide, called Bβ15-42, which is cleaved by plasmin from the end of the fibrin Bβ-chain, is protective in myocardial and renal ischemia/reperfusion injury and improves the outcome in experimental sepsis. Bβ15-42 has been shown to mediate different beneficial effects in endothelial cells throu...
Source: Translational Research : the journal of laboratory and clinical medicine - July 25, 2019 Category: Laboratory Medicine Authors: Sörensen-Zender I, Chen R, Song R, David S, Melk A, Haller H, Schmitt R Tags: Transl Res Source Type: research
NFIC promotes the vitality and osteogenic differentiation of rat dental follicle cells
In conclusion, these results suggested that upregulation of NFIC may promote the proliferation and osteogenic/cementogenic differentiation of DFCs.
Source: Journal of Molecular Histology - August 19, 2019 Category: Laboratory Medicine Source Type: research
Circulating ectodysplasin A is a potential biomarker for nonalcoholic fatty liver disease
ConclusionsEDA aggravates steatosis by striking balance between lipid deposition and elimination. It was a potential biomarker of NAFLD.
Source: Clinica Chimica Acta - September 15, 2019 Category: Laboratory Medicine Source Type: research
Proteomic Comparison of Malignant Human Germ Cell Tumor Cell Lines.
Authors: Bremmer F, Bohnenberger H, Küffer S, Oellerich T, Serve H, Urlaub H, Strauss A, Maatoug Y, Behnes CL, Oing C, Radzun HJ, Ströbel P, Balabanov S, Honecker F
Abstract
Malignant germ cell tumors (GCT) are the most common malignant tumors in young men between 18 and 40 years. The correct identification of histological subtypes, in difficult cases supported by immunohistochemistry, is essential for therapeutic management. Furthermore, biomarkers may help to understand pathophysiological processes in these tumor types. Two GCT cell lines, TCam-2 with seminoma-like characteristics, and NTERA-2, an embryonal car...
Source: Disease Markers - October 2, 2019 Category: Laboratory Medicine Tags: Dis Markers Source Type: research
The Regulatory Effects of CDKN1A on Relevant Genes in Kaposi's Sarcoma Cells.
Abstract
The cyclin-dependent kinase inhibitor 1A (CDKN1A) gene plays important roles in different types of cancer; however, its mechanism in Kaposi's Sarcoma (KS) is far less known. The aim of this study is to investigate the regulatory effects of CDKN1A on relevant genes in KS cells, and ultimately determine the role of CDKN1A in KS. In the study, the CDKN1A overexpression group, or siRNA group, was transfected into KS cells, respectively. Western-Blot (WB) and quantitative real-time polymerase chain reactions (q-PCR) were performed to detect the expression of cell cycle protein D1 (CyclinD1) and cell cycle prot...
Source: Annals of Clinical and Laboratory Science - August 31, 2019 Category: Laboratory Medicine Authors: Zhang J, Pu XM, Wu XJ Tags: Ann Clin Lab Sci Source Type: research
Evaluation of CERS2 Gene as a Potential Biomarker for Bladder Cancer.
This study demonstrates that CERS2 is differentially expressed in different types of bladder cancer cell lines and that the siRNA-mediated downregulation of the expression of CERS2 reduces the migratory potential of UMUC1 bladder cancer cells. However, there were no significant correlations between the expression levels of the CERS2 protein with bladder cancer grade/stage or between the IC50 values of cells treated with NDV and CERS2 expression. Although the utility of CERS2 expression may be limited, its potential as an antimigration cancer therapeutic should be further examined.
PMID: 31636736 [PubMed - in process]
Source: Disease Markers - October 24, 2019 Category: Laboratory Medicine Tags: Dis Markers Source Type: research
Treating PMP22 gene duplication-related Charcot-Marie-Tooth disease: The past, the present and the future.
Abstract
Charcot-Marie-Tooth (CMT) disease is the most frequent inherited neuropathy, affecting 1/1500 to 1/10000. CMT1A represents 60-70% of all CMT and is caused by a duplication on chromosome 17p11.2 leading to an overexpression of the Peripheral Myelin Protein 22 (PMP22). PMP22 gene is under tight regulation and small changes in its expression influences myelination and affect motor and sensory functions. To date, CMT1A treatment is symptomatic and classic pharmacological options have been disappointing. Here, we review the past, present and future treatment options for CMT1A, with a special emphasis on the hi...
Source: Translational Research : the journal of laboratory and clinical medicine - July 17, 2020 Category: Laboratory Medicine Authors: Boutary S, Echaniz-Laguna A, Adams D, Loisel-Duwattez J, Schumacher M, Massaad C, Massaad-Massade L Tags: Transl Res Source Type: research
Overexpression of Spondin-2 Is Associated with Recurrence-Free Survival in Patients with Localized Clear Cell Renal Cell Carcinoma.
Conclusion: SPON2 expression might function as a prognostic biomarker in patients with localized ccRCC.
PMID: 32952742 [PubMed - in process]
Source: Disease Markers - September 22, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research
The antidiabetic drug glibenclamide exerts direct retinal neuroprotection.
Abstract
Sulfonylureas, widely used as hypoglycaemic agents in adults with type 2 diabetes, have neuroprotective effects in preclinical models of central nervous system injury, and in children with neuropsychomotor impairments linked to neonatal diabetes secondary to ATP-sensitive potassium channel mutations. In the human and rodent retina, we show that the glibenclamide activated channel SUR1 is expressed in the retina and enriched in the macula; we also show that it co-localizes with the potassium channel Kir6.2, and with the cation channel transporter TRPM4. Glibenclamide (glyburide), administered at doses that...
Source: Translational Research : the journal of laboratory and clinical medicine - October 17, 2020 Category: Laboratory Medicine Authors: Berdugo M, Delaunay K, Naud MC, Guegan J, Moulin A, Savoldelli M, Picard E, Radet L, Jonet L, Djerada Z, Gozalo C, Daruich A, Beltrand J, Jeanny JC, Kermorvant-Duchemin E, Crisanti P, Polak M, Behar-Cohen F Tags: Transl Res Source Type: research
TRIP13 promotes the proliferation and invasion of lung cancer cells via the Wnt signaling pathway and epithelial –mesenchymal transition
This study found that TRIP13 can co-localize and bind with LRP6. Furthermore, overexpression of TRIP13 caused the upregulation of N-cadherin, Snail, and vimentin, and the downregulation of E-cadherin (p < 0.05). The aforementioned results were reversed after knocking down the expression of TRIP13 (p < 0.05). TRIP13 is highly expressed in lung cancers, indicating poor prognosis. overexpression of TRIP13 promotes the proliferative and invasive ability of lung cancer cells via the activation of Wnt signaling pathway and EMT.
Source: Journal of Molecular Histology - October 30, 2020 Category: Laboratory Medicine Source Type: research
MicroRNA-216a Promotes Endothelial Inflammation by Smad7/I κBα Pathway in Atherosclerosis.
Conclusion: In summary, our findings suggest a new mechanism of vascular endothelial inflammation involving Smad7/IκBα signalling pathway in atherosclerosis.
PMID: 33282009 [PubMed - in process]
Source: Disease Markers - December 8, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research
Role of Estrogen Receptor-Mediated Anti-Tumor Effects in U2OS Cells.
CONCLUSIONS: 17β-estradiol exhibited significant anti-tumor effects on U2OS cells that were mediated by ERs and reduced cell proliferation, migration, and invasion.
PMID: 33334789 [PubMed - in process]
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Yang Z, Qian J, Yu W, Yang M, Liu B, Tao H Tags: Ann Clin Lab Sci Source Type: research
