High JAK2 Protein Expression Predicts a Poor Prognosis in Patients with Resectable Pancreatic Ductal Adenocarcinoma.
Conclusions: Assessment of JAK2 protein expression may be a promising method to predict prognosis in patients with resectable PDAC. PMID: 33029256 [PubMed - in process] (Source: Disease Markers)
Source: Disease Markers - October 9, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Relationship between Plasma Lipoprotein-Associated Phospholipase A2 Concentrations and Apolipoprotein in Stable Coronary Artery Disease Patients.
Authors: Ling Y, Tang S, Cao Y, Fu C Abstract Background: Increasing evidence states that the plasma lipoprotein-associated phospholipase A2 (Lp-PLA2) levels and apolipoprotein particles are regarded as the risk maker for cardiovascular heart disease. Nevertheless, the issue about whether Lp-PLA2 is associated with apolipoprotein particles in individuals who have been diagnosed as stable coronary artery disease (CAD) remains largely unexplored. Method: All 569 participants engaged in this research, who never took lipid-lowering drugs, had been divided into groups by the coronary angiography (CAG), namely, stabl...
Source: Disease Markers - October 9, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

eRNAs and Superenhancer lncRNAs Are Functional in Human Prostate Cancer.
Authors: Zhang X, Pang P, Jiang M, Cao Q, Li H, Xu Y, Li Y, Chen X, Han J Abstract Prostate cancer (PCa) is one of the most commonly diagnosed cancers in males worldwide. lncRNAs (long noncoding RNAs) play a significant role in the occurrence and development of PCa. eRNAs (enhancer RNAs) and SE-lncRNAs (superenhancer lncRNAs) are important elements of lncRNAs, but the role of eRNAs and SE-lncRNAs in PCa remains largely unclear. In this work, we identified 681 eRNAs and 292 SE-lncRNAs that were expressed differentially in PCa using a microarray. We also found that eRNAs transcribed from active open chromatin had sig...
Source: Disease Markers - October 9, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Evaluation of Altered Glutamatergic Activity in a Piglet Model of Hypoxic-Ischemic Brain Damage Using 1H-MRS.
Authors: Dang Y, Wang X Abstract Methods: Twenty-five newborn piglets were selected and then randomly assigned to the control group (n = 5) and the model group (n = 20) subjected to HI. HI was induced by blocking bilateral carotid blood flow under simultaneous inhalation of a 6% oxygen mixture. 1H-MRS data were acquired from the basal ganglia at the following time points after HI: 6, 12, 24, and 72 h. Changes in protein levels of EAAT2 and GluR2 were determined by immunohistochemical analysis. Correlations among metabolite concentrations, metabolite ratios, and the protein levels of EAAT2 and GluR2 were inve...
Source: Disease Markers - October 9, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Genetic Polymorphisms of MMP1, MMP9, COL1A1, and COL1A2 in Polish Patients with Thoracic Aortopathy.
Conclusion: The results of our study did not support associations between MMP1, MMP9, COL1A1, and COL1A2 genetic variants with the risk of thoracic artery disease in Polish patients. However, rs1799750 MMP1 and rs3918242 MMP9 seem to be associated with the degree of aortic regurgitation. PMID: 33029260 [PubMed - in process] (Source: Disease Markers)
Source: Disease Markers - October 9, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Fecal Microbiome Alteration May Be a Potential Marker for Gastric Cancer.
In this study, we sequenced the 16S rRNA and 18S rRNA genes of fecal bacteria and fungi, respectively, in 134 gastric cancer patients and 58 healthy controls matched by age and gender. Propensity score matching (PSM) was adopted for adjusting diet habits and lifestyle, and 44 patients and 44 healthy controls (matching population) were enrolled. Serum antibody to H. pylori and metabolites of the matching population were detected. The positive rates of antibody to H. pylori between the patients and the control group did not reach the statistical difference. LEfSe analysis indicated that bacteria were more stable than fungi w...
Source: Disease Markers - October 6, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Digital PCR for the Analysis of MYC Copy Number Variation in Lung Cancer.
Authors: Brik A, Weber DG, Casjens S, Rozynek P, Meier S, Behrens T, Stamatis G, Darwiche K, Theegarten D, Brüning T, Johnen G Abstract Background: MYC (v-myc avian myelocytomatosis viral oncogene homolog) is one of the most frequently amplified genes in lung tumors. For the analysis of gene copy number variations, dPCR (digital PCR) is an appropriate tool. The aim of our study was the assessment of dPCR for the detection of MYC copy number variations (CNV) in lung tissue considering clinicopathological parameters. Material and Methods. MYC status was analyzed with dPCR as well as qPCR (quantitative PCR) using...
Source: Disease Markers - October 6, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Clinicopathological Significance of Neuropilin 1 Expression in Gastric Cancer: A Meta-Analysis.
Conclusion: The expression of NRP1 protein in gastric cancer is closely correlated to clinical stage, tumor size, TNM stage, differentiation, and lymph node metastasis. PMID: 33014187 [PubMed - in process] (Source: Disease Markers)
Source: Disease Markers - October 6, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

A Novel Gene Signature to Predict Survival Time and Incident Ventricular Arrhythmias in Patients with Dilated Cardiomyopathy.
Authors: Ge C, He Y Abstract The mortality in nonischaemic dilated cardiomyopathy (NIDCM) patients is still at a high level; sudden death in NIDCM can be caused by ventricular tachycardia. It is necessary to explore the pathogenesis of ventricular arrhythmias (VA) in NIDCM. Differentially expressed genes (DEGs) were identified by comparing the gene expression of NIDCM patients with or without VA in the gene expression profile of GSE135055. A total of 228 DEGs were obtained, and 3 genes were screened out to be significantly related to the survival time of NIDCM patients. We established a prediction model on two-gene...
Source: Disease Markers - October 6, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Diagnostic and Prognostic Potentials of Long Noncoding RNA ELF3-AS1 in Glioma Patients.
Authors: Mei JC, Yan G, Mei SQ Abstract Objective: Accumulating evidence implies that long noncoding RNAs (lncRNAs) play a crucial role in predicting survival for glioma patients. However, the potential function of lncRNA ELF3-antisense RNA 1 (ELF3-AS1) in tumors remained largely unclear. The aim of this study was to explore the expression of lncRNA ELF3-antisense RNA 1 (ELF3-AS1) and evaluate its functions in glioma patients. Patients and Methods. ELF3-AS1 expressions were examined by RT-PCR in 182 pairs of glioma specimens and adjacent normal tissues. The receiver operating characteristic (ROC) curve was performe...
Source: Disease Markers - October 6, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Vitamin D Deficiency in Autism Spectrum Disorder: A Cross-Sectional Study.
Authors: Petruzzelli MG, Marzulli L, Margari F, De Giacomo A, Gabellone A, Giannico OV, Margari L Abstract Vitamin D plays a role in central nervous system (CNS) development. Recent literature focused on vitamin D status in children and adolescents with autism spectrum disorder (ASD), but with inconsistent results. Our case-control study is aimed at evaluating serum 25-hydroxyl-vitamin D (25(OH)D) concentration in children with ASD (ASD group, n = 54) compared to children affected by other neurological and psychiatric disorders (non-ASD group, n = 36). All patients were admitted at the Complex Operative Unit of Chi...
Source: Disease Markers - October 6, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Coexpression of Matrix Metalloproteinase-7 and Tissue Inhibitor of Metalloproteinase-1 as a Prognostic Biomarker in Gastric Cancer.
Conclusions: In conclusion, coexpression of MMP-7 and its inhibitor TIMP-1 in gastric tumour tissues is a potential prognostic marker for GC. Greater knowledge of protein expression will lead to new paradigms and possible improvements in therapeutics. PMID: 33005257 [PubMed - in process] (Source: Disease Markers)
Source: Disease Markers - October 3, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Clinical Significance of C-Reactive Protein to Albumin Ratio in Patients with Hepatocellular Carcinoma: A Meta-Analysis.
Authors: Lin N, Li J, Ke Q, Wang L, Cao Y, Liu J Abstract Aim: To evaluate the prognostic significance of C-reactive protein to albumin ratio (CAR) for clinical outcomes in hepatocellular carcinoma (HCC) patients. Material and Methods. Eligible studies were searched by PubMed, MedLine, the Cochrane Library, from January 1, 2000, to June 30, 2019, investigating the prognostic value of CAR in patients with HCC. Primary endpoint was OS. Hazard ratio (HR) with 95% confidence interval (CI) was used to determine the effect size. Results: 7 records including 2208 patients published since 2014 were enrolled into our me...
Source: Disease Markers - September 25, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

6,12-Diphenyl-3, 9-diazatetraasterane-1, 5, 7, 11-tetracarboxylate Inhibits Proliferation, Migration and Promotes Apoptosis in Ovarian Cancer Cells.
Authors: Chen P, Wang H, Li M, Yang L, Mou F, Singh B, He JY, Zhang W Abstract 6,12-Diphenyl-3,9-diazatetraasterane-1, 5, 7, 11-tetracarboxylate (DDTC) has been synthesized by the photodimerization of 4-phenyl-1,4-dihydropyridine-3,5-dicarboxylate. The potential of theercvantitumor activity and mechanism were investigated in vitro using MTT assay in human lung cancer cell line A549, ovarian cancer cell lines SKOV3 and A2780, breast cancer cell line MCF-7, gastric cancer cell line BGC-823, colon cancer cell line HT29, prostate cancer cell line DU145, and liver cancer cell line SMMC7721. The results show that DDTC ca...
Source: Disease Markers - September 25, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Updating a Strategy for Histone Deacetylases and Its Inhibitors in the Potential Treatment of Cerebral Ischemic Stroke.
Conclusions: HDAC may be a new biological target for cerebral ischemic stroke. Future drug development targeting HDAC may make it a potentially effective anticerebral ischemic stroke drug. PMID: 32963637 [PubMed - in process] (Source: Disease Markers)
Source: Disease Markers - September 25, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Impacts of Respiratory Muscle Training on Respiratory Functions, Maximal Exercise Capacity, Functional Performance, and Quality of Life in School-Aged Children with Postoperative Congenital Diaphragmatic Hernia.
This study is a randomized control study. 40 children with CDH (age: 9-11 years) were assigned randomly into two groups. The first group conducted an incentive spirometer exercise combined with inspiratory muscle training (study group, n = 20), whereas the second group conducted only incentive spirometer exercise (control group, n = 20), thrice weekly for twelve consecutive weeks. Respiratory functions, maximal exercise capacity, functional performance, and pediatric quality of life inventory (PedsQL) were assessed before and after the treatment program. Results. Regarding the posttreatment analysis, the study group showed...
Source: Disease Markers - September 25, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Overexpression of Spondin-2 Is Associated with Recurrence-Free Survival in Patients with Localized Clear Cell Renal Cell Carcinoma.
Authors: Ma HM, Yu M, Wu C, Huang HB, Li YW, Zhang P, Huang JJ, Cheng L, Feng G, Li GR Abstract Background: The spondin-2 (SPON2) gene is overexpressed in multiple malignant tumors and may promote tumor aggressiveness. However, its expression profile and functional roles in clear cell renal cell carcinoma (ccRCC) are still unclear. Methods: SPON2 expression in ccRCC was evaluated using expression data from TCGA and GEO databases, then confirmed by local patient population (94 patients). The clinical significance of SPON2 expression was evaluated. Downregulation of SPON2 was performed using small-interfering RNA...
Source: Disease Markers - September 22, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Identification of Key Genes of Prognostic Value in Clear Cell Renal Cell Carcinoma Microenvironment and a Risk Score Prognostic Model.
Authors: Zhao E, Bai X Abstract Objective: We aimed at identifying the key genes of prognostic value in clear cell renal cell carcinoma (ccRCC) microenvironment and construct a risk score prognostic model. Materials and Methods: Immune and stromal scores were calculated using the ESTIMATE algorithm. A total of 539 ccRCC cases were divided into high- and low-score groups. The differentially expressed genes in immune and stromal cells for the prognosis of ccRCC were screened. The relationship between survival outcome and gene expression was evaluated using univariate and multivariate Cox proportional hazard regre...
Source: Disease Markers - September 22, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Identification of Key Prognostic Biomarker and Its Correlation with Immune Infiltrates in Pancreatic Ductal Adenocarcinoma.
In conclusion, our results suggest that nine upregulated biomarkers are related to poor prognosis in PDAC and may serve as potential prognostic biomarkers for PDAC therapy. Furthermore, SPARC, COL6A3, and FBN1 play an important role in tumor-related immune infiltration and may be ideal targets for immune therapy against PDAC. PMID: 32934754 [PubMed - in process] (Source: Disease Markers)
Source: Disease Markers - September 18, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Evidence of the Prognostic Value of Pretreatment Systemic Inflammation Response Index in Cancer Patients: A Pooled Analysis of 19 Cohort Studies.
Conclusion: High SIRI is associated with unfavorable clinical outcomes in human malignancies; pretreatment SIRI level might be a useful and promising predictive indicator of prognosis in cancers. PMID: 32934755 [PubMed - in process] (Source: Disease Markers)
Source: Disease Markers - September 18, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Pharmacogenomics Study for Raloxifene in Postmenopausal Female with Osteoporosis.
In this study, a total of 41 postmenopausal osteoporosis patients under regular raloxifene treatment were included. Gene-based analysis using MAGMA was applied to investigate the genetic association with the bone mineral density response to raloxifene at the lumbar spine or femoral neck site. Results from gene-based analysis indicated several genes (GHRHR, ABHD8, and TMPRSS6) related to the responses of raloxifene. Besides, the pathways of iron ion homeostasis, osteoblast differentiation, and platelet morphogenesis were enriched which implies that these pathways might be relatively susceptible to raloxifene treatment outco...
Source: Disease Markers - September 18, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Gut Microbiota Profile in Patients with Type 1 Diabetes Based on 16S rRNA Gene Sequencing: A Systematic Review.
This study is aimed at systematically reviewing the existing literature, which has investigated the alterations of the intestinal microbiome in T1D patients compared with healthy controls (HCs) using 16S ribosomal RNA-targeted sequencing. The databases of MEDLINE, EMBASE, Web of Science, and the Cochrane Library were searched until April 2019 for case-control studies comparing the composition of the intestinal microbiome in T1D patients and HCs based on 16S rRNA gene sequencing techniques. The Newcastle-Ottawa Scale was used to assess the methodological quality. Ten articles involving 260 patients with T1D and 276 HCs were...
Source: Disease Markers - September 12, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

A Comprehensive Exploration of the lncRNA CCAT2: A Pan-Cancer Analysis Based on 33 Cancer Types and 13285 Cases.
Authors: Huang B, Yu M, Guan R, Liu D, Hou B Abstract Whether the lncRNA CCAT2 expression level affects the clinical progression and outcome of cancer patients has not yet been fully elucidated. There is still an inconsistent view regarding the correlation between CCAT2 expression and clinicopathological factors, including survival data. Besides, the regulation mechanism of CCAT2 in human cancer is still unclear. Our study analyzed a large number of publication data and TCGA databases to identify the association of CCAT2 expression with clinicopathological factors and to explore the regulatory mechanisms in human c...
Source: Disease Markers - September 12, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Serum Small Proline-Rich Protein 2A (SPRR2A) Is a Noninvasive Biomarker in Gastric Cancer.
Authors: Xu X, Wei S, Chen Y, Yu D, Wang X, Dong X Abstract Objective: Since early diagnosis is very important for treating gastric cancer (GC), we aimed to detect serum small proline-rich protein2A (SPRR2A) to verify its diagnostic value for GC patients. Methods: Serum samples were collected from 200 patients with GC, 100 patients with gastritis, 40 patients with rectal cancer (RC), 50 patients with colon cancer (CC), and 100 healthy controls. An enzyme-linked immunosorbent assay (ELISA) detection kit was applied to measure serum SPRR2A concentration. The correlations between serum SPRR2A and carcinoembryonic ...
Source: Disease Markers - September 12, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Serum and Urine Neutrophil Gelatinase-Associated Lipocalin Levels Measured at Admission Predict Progression to Chronic Kidney Disease in Sepsis-Associated Acute Kidney Injury Patients.
Authors: Tuan PNH, Quyen DBQ, Van Khoa H, Loc ND, Van My P, Dung NH, Toan ND, Quyet D, Thang LV Abstract Background: To evaluate the ratio of acute kidney injury (AKI) to chronic kidney disease (CKD) in sepsis-associated acute kidney injury (SA-AKI) patients of the intensive care unit (ICU) and predictive value of neutrophil gelatinase-associated lipocalin (NGAL) measured at the admission time in the progression of AKI to CKD. Methods: A study of 121 consecutive adult patients admitted to the intensive care unit (ICU) diagnosed as SA-AKI. AKI and CKD were defined based on Kidney Disease: Improving Global Outcom...
Source: Disease Markers - September 12, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Sox10 Is a Specific Biomarker for Neural Crest Stem Cells in Immunohistochemical Staining in Wistar Rats.
Conclusions: Sox10 is the specific marker for immunohistochemical staining of NCSCs in paraffin sections. In cultured cells, Sox10, p75NTR, and RET presented a similar staining effect. PMID: 32908618 [PubMed - in process] (Source: Disease Markers)
Source: Disease Markers - September 12, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Urinary Peptides Associated Closely with Gestational Diabetes Mellitus.
Authors: Hu Z, Tian Y, Li J, Hu M, Zhang M Abstract Gestational diabetes mellitus (GDM) is a common disease of pregnant women, which has a higher incidence in recent years. The purpose of this study is to explore urinary biomarkers that could predict and monitor gestational diabetes mellitus (GDM). Urine samples from 30 normal pregnant women and 78 GDM patients were collected and purified by weak cationic exchange magnetic beads (MB-WCX), then analyzed by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). The urinary peptide signatures of the two groups were compared by Bio...
Source: Disease Markers - September 11, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Peripheral Blood Occludin Level as a Biomarker for Perioperative Cerebral Edema in Patients with Brain Tumors.
The objective is to explore the effectiveness of serum occludin on predicting the extent of perioperative brain edema and outcome in patients with brain tumors. Methods: This prospective study enrolled 55 patients with brain tumors and 24 healthy controls in Sanbo Brain Hospital from June 2019 through November 2019. Serum occludin levels were measured preoperatively and on postoperative day 1. Peritumoral edema was assessed preoperatively using MRI. Pericavity brain edema on postoperative day 1 was evaluated using CT. Results: Compared with healthy controls, the serum occludin level was higher in patients with brai...
Source: Disease Markers - September 6, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Investigation of Lipid Profile and Clinical Manifestations in SCA Children.
Conclusions: In sum, our results suggest that total cholesterol, HDL-C, and LDL-C levels are associated with hemolysis and anemia markers and, most importantly, with clinical complications related to vasculopathy in SCA. PMID: 32884585 [PubMed - in process] (Source: Disease Markers)
Source: Disease Markers - September 6, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Detection of BRAF V600E Mutation in Ganglioglioma and Pilocytic Astrocytoma by Immunohistochemistry and Real-Time PCR-Based Idylla Test.
Authors: Durślewicz J, Klimaszewska-Wiśniewska A, Antosik P, Kasperska A, Grzanka D, Szylberg T, Szylberg Ł Abstract The BRAF V600E mutation is an important oncological target in certain central nervous system (CNS) tumors, for which a possible application of BRAF-targeted therapy grows continuously. In the present study, we aim to determine the prevalence of BRAF V600E mutations in a series of ganglioglioma (GG) and pilocytic astrocytoma (PA) cases. Simultaneously, we decided to verify whether the combination of fully automated tests-BRAF-VE1 immunohistochemistry (IHC) and Idylla BRAF mutation assay-may be usef...
Source: Disease Markers - September 5, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Serum Actinin-4 Levels as a Potential Diagnostic and Prognostic Marker in Cervical Cancer.
Authors: Ma X, Xue H, Zhong J, Feng B, Zuo Y Abstract Purpose: The present study was aimed at determining the serum levels of actinin-4 (ACTN4) in cervical cancer (CC) and investigating the diagnostic and prognostic value of serum ACTN4 in CC. Materials and Methods: We included 93 CC patients, 52 cervical intraepithelial neoplasia (CIN) patients, and 70 healthy women. Serum ACTN4 levels were assessed using an ELISA method. A receiver operating characteristic (ROC) curve was performed to evaluate the diagnostic value of serum ACTN4. The survival curves were used to display the overall survival distributions. ...
Source: Disease Markers - August 30, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

The Value of Haematological Parameters and Tumour Markers in the Prediction of Intestinal Obstruction in 1474 Chinese Colorectal Cancer Patients.
Authors: Cao Y, Ke S, Gu J, Mao F, Yao S, Deng S, Yan L, Wu K, Liu L, Cai K Abstract Intestinal obstruction, a life-threatening problem, often occurs in patients with advanced colorectal cancer (CRC). However, the cause of obstruction is still unknown. Very few prediction models for intestinal obstruction in CRC exist, and their results are unreliable. Therefore, we investigated whether preoperative serum tumour markers (STMs) combined with haematological and biochemical markers could be used as predictors. We retrospectively analysed 1474 patients with CRC who underwent radical resection after admission. Several c...
Source: Disease Markers - August 30, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Period Family of Clock Genes as Novel Predictors of Survival in Human Cancer: A Systematic Review and Meta-Analysis.
This study was the first to collect the relevant publications to systematically evaluate the value of the expression of the PER gene family in the prediction of survival and prognosis of human tumors. Methods: The PubMed, Cochrane Library, Embase, and Web of Science databases were searched systematically, and a meta-analysis was performed. Results: A total of 12 eligible publications met the inclusion criteria for the meta-analysis, including 1,369 patients and 9 different types of cancer. The pooled hazard ratio for overall survival indicated that the overall survival of patients in the high PER1, PER2, and PER3 p...
Source: Disease Markers - August 29, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Exosomes in Nephropathies: A Rich Source of Novel Biomarkers.
Authors: Masaoutis C, Al Besher S, Koutroulis I, Theocharis S Abstract The biomarkers commonly utilized in diagnostic evaluations of kidney disease suffer from low sensitivity, especially in the early stages of renal damage. On the other hand, obtaining a renal biopsy to augment clinical decision making can lead to potentially serious complications. In order to overcome the shortcomings of currently available diagnostic tools, recent studies suggest that exosomes, cell-secreted extracellular vesicles containing a large array of active molecules to facilitate cell-to-cell communication, may represent a rich source o...
Source: Disease Markers - August 29, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Investigating the GWAS-Implicated Loci for Rheumatoid Arthritis in the Pakistani Population.
Authors: Aslam MM, John P, Fan KH, Bhatti A, Aziz W, Ahmed B, Feingold E, Demirci FY, Kamboh MI Abstract Rheumatoid arthritis (RA) is a complex and multifactorial autoimmune disorder with the involvement of multiple genetic and environmental factors. Genome-wide association studies (GWAS) have identified more than 50 RA genetic loci in European populations. Given the anticipated overlap of RA-relevant genes and pathways across different ethnic groups, we sought to replicate 58 GWAS-implicated SNPs reported in Europeans in Pakistani subjects. 1,959 unrelated subjects comprising 1,222 RA cases and 737 controls were c...
Source: Disease Markers - August 26, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

The Variation of Peripheral Inflammatory Markers in Vocal Leukoplakia before and after Recurrence and Canceration.
Conclusions: NLR, PLR, and MLR are reliable biomarkers in the circulation system which show significantly interrelation with the pathological progression of vocal fold leukoplakia. Presurgical evaluation of NLR, PLR, and MLR may have potential values to indicate the following treatment in clinical practice. PMID: 32831972 [PubMed - in process] (Source: Disease Markers)
Source: Disease Markers - August 26, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Association of Serum FAM19A5 with Cognitive Impairment in Vascular Dementia.
Authors: Li J, Li S, Song Y, Zhou W, Zhu X, Xu S, Ma Y, Zhu C Abstract Objective: Family with sequence similarity 19 member A5 (FAM19A5), a novel chemokine-like peptide, is a secreted protein mainly expressed in the brain. FAM19A5 was recently found to be involved in a variety of neurological diseases; however, its correlation with vascular dementia (VaD) remains unclear. The aim of the study is to explore the association between serum FAM19A5 and cognitive impairment in subjects with VaD. Method: 136 VaD subjects and 81 normal controls were recruited in the study. Their demographic and clinical baseline data w...
Source: Disease Markers - August 26, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Association of TLR-2 Gene Polymorphisms with the Risk of Periodontitis: A Meta-Analysis.
Conclusion: The TLR-2 rs1898830, rs5743708 polymorphism may be associated with susceptibility to periodontitis. In the future, genome-wide approaches and large-scale, multiethnic case-control trials are still needed. PMID: 32831974 [PubMed - in process] (Source: Disease Markers)
Source: Disease Markers - August 26, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Neoadjuvant Chemotherapy Followed by Radical Surgery versus Radiotherapy (with or without Chemotherapy) in Patients with Stage IB2, IIA, or IIB Cervical Cancer: A Systematic Review and Meta-Analysis.
This study was to compare the efficacy and safety between neoadjuvant chemotherapy followed by radical surgery (NACT+RS) and radiotherapy only (RT) or concurrent chemoradiotherapy (CCRT) for treatment of patients with stage IB2, IIA, or IIB cervical cancer. Method: The electronic databases of PubMed, Embase, and the Cochrane Library were searched to screen relevant studies from their inception to October 2018. Clinical data including overall survival (OS), disease-free survival (DFS), and adverse events were extracted. Egger's test was used to evaluate the publication bias, and sensitivity analysis was conducted to est...
Source: Disease Markers - August 19, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Review of Serum Biomarkers and Models Derived from Them in HBV-Related Liver Diseases.
Authors: Wu J, Mao W Abstract A series of predictive scoring systems is available for stratifying the severity of conditions and assessing the prognosis in patients with HBV-related liver diseases. We show nine of the most popular serum biomarkers and their models (i.e., serum cystatin C, homocysteine, C-reactive protein, C-reactive protein to albumin ratio, aspartate aminotransferase to platelet ratio index, fibrosis index based on four factors, gamma-glutamyl transpeptidase to platelet ratio, albumin-bilirubin score, and gamma-glutamyl transpeptidase to albumin ratio) that have gained great interest from clinicia...
Source: Disease Markers - August 13, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Increased Serum Level and High Tissue Immunoexpression of Interleukin 17 in Cutaneous Lichen Planus: A Novel Therapeutic Target for Recalcitrant Cases?
Authors: Żychowska M, Batycka-Baran A, Baran W Abstract Background: Interleukin-17 is supposed to play an important role in the pathogenesis of oral lichen planus (OLP). However, there is scarce data in the literature on its significance in the cutaneous variant of the disease. Objectives: To determine the serum level and tissue immunoexpression of IL-17 in cutaneous lichen planus (CLP). Methods: Fifty-two adult patients with CLP, without any significant autoimmune or inflammatory conditions, were included in the first part of the study. The control group consisted of 27 age- and sex-matched healthy volunt...
Source: Disease Markers - August 13, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Molecular Markers for Detecting Schistosoma Species by Loop-Mediated Isothermal Amplification.
Authors: Fernández-Soto P, Avendaño C, Sala-Vizcaíno A, Crego-Vicente B, Febrer-Sendra B, García-Bernalt Diego J, Oleaga A, López-Abán J, Vicente B, Patarroyo MA, Muro A Abstract Schistosomiasis is considered a neglected parasitic disease. Around 280,000 people die from it annually, and more than 779 million people are at risk of getting infected. The schistosome species which infect human beings are Schistosoma mansoni, Schistosoma haematobium, Schistosoma intercalatum, Schistosoma japonicum, Schistosoma guineensis, and Schistosoma mekongi. This disease is also of veterina...
Source: Disease Markers - August 13, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Association of the Expression Level of miR-16 with Prognosis of Solid Cancer Patients: A Meta-Analysis and Bioinformatic Analysis.
Conclusion: Low expression level of miR-16 is an indicator for poor prognosis of solid cancer patients, particularly in reproductive system cancers. PMID: 32774515 [PubMed - in process] (Source: Disease Markers)
Source: Disease Markers - August 13, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Radixin Relocalization and Nonmuscle α-Actinin Expression Are Features of Remodeling Cardiomyocytes in Adult Patients with Dilated Cardiomyopathy.
Conclusions: Remodeling was similar in ARC and in cardiomyocytes of DCM suggesting evolutionary conserved mechanisms of regeneration. Despite activation of fetal genes, the atrophy of ARC indicates differences in their regenerative capacity from NRC. Cardiac-derived factors induced NM-actinin expression and increased survival of ischemic ARC while circulating molecules were less effective. Identification of these cardiac-derived factors and determination of their individual capacity to heal or damage are of particular importance for a biomarker-guided therapy in adult patients. PMID: 32774516 [PubMed - in process] (Sou...
Source: Disease Markers - August 13, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Low UGP2 Expression Is Associated with Tumour Progression and Predicts Poor Prognosis in Hepatocellular Carcinoma.
Authors: Hu Q, Shen S, Li J, Liu L, Liu X, Zhang Y, Zhou Y, Zhu W, Yu Y, Cui G Abstract Hepatocellular carcinoma (HCC) is a malignant tumour associated with a high mortality rate and poor prognosis worldwide. Uridine diphosphate-glucose pyrophosphorylase 2 (UGP2), a key enzyme in glycogen biosynthesis, has been reported to be associated with the occurrence and development of various cancer types. However, its diagnostic value and prognostic value in HCC remain unclear. The present study observed that UGP2 expression was significantly downregulated at both the mRNA and protein levels in HCC tissues. Receiver operati...
Source: Disease Markers - August 2, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

A Circulating Long Noncoding RNA Panel Serves as a Diagnostic Marker for Hepatocellular Carcinoma.
Conclusion: The panel of serum Linc00152, UCA1, and AFP demonstrates a novel and noninvasive biomarker with relatively high sensitivity and specificity for HCC diagnosis. PMID: 32733618 [PubMed - in process] (Source: Disease Markers)
Source: Disease Markers - August 2, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Prognostic Value of Admission Mean Corpuscular Volume for Major Adverse Cardiovascular Events following Stent Implantation in Nondiabetic and Diabetic Patients with Acute Coronary Syndrome.
Conclusion: In patients with ACS, elevated admission MCV is an important and independent predictor for MACEs following stent implantation, especially amongst those without DM even after adjusting for lifestyle and clinical risk factors. However, as the follow-up period increased, the admission MCV lost its ability to predict MACEs. PMID: 32733619 [PubMed - in process] (Source: Disease Markers)
Source: Disease Markers - August 2, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Prognostic Value of DNA Methylation-Driven Genes in Clear Cell Renal Cell Carcinoma: A Study Based on Methylation and Transcriptome Analyses.
Conclusion: This study provided an important bioinformatics foundation for in-depth studies of ccRCC DNA methylation. PMID: 32733620 [PubMed - in process] (Source: Disease Markers)
Source: Disease Markers - August 2, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Targeted Metabolomics for Plasma Amino Acids and Carnitines in Patients with Metabolic Syndrome Using HPLC-MS/MS.
Authors: Gong LL, Yang S, Zhang W, Han FF, Xuan LL, Lv YL, Liu H, Liu LH Abstract Metabolic syndrome (MetS) is a health disorder characterized by metabolic abnormalities that predict an increased risk to develop cardiovascular disease (CVD) and type 2 diabetes. Biomarkers can provide an insight into the novel mechanism for MetS and can be potentially used for personalized response to therapies. We exploited a targeted HPLC-MS/MS method to characterize plasma amino acids and carnitine metabolic profile in MetS patients. A training set (40 cases and 40 controls) and validation set (80 MetS patients and 80 healthy con...
Source: Disease Markers - August 2, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research

Association of Uric Acid in Serum and Urine with Arterial Stiffness: Hanzhong Adolescent Hypertension Study.
Authors: Wang Y, Zhang XY, Gao WH, Du MF, Chu C, Wang D, Chen C, Yuan Y, Ma Q, Liao YY, Yan Y, Wang KK, Zhang J, Gao K, Li CH, Li H, Ma Q, Hu JW, Zou T, Sun Y, Li M, Zhou HW, Jia H, Mu JJ Abstract Background: Hyperuricemia has long been associated with increased cardiovascular risk, and arterial stiffness is proposed as a mediator. The present study is aimed at examining the associations of uric acid (UA) in blood and urine with arterial stiffness in a Chinese cohort. Methods: A total of 2296 participants (mean age: 43.0 years) from our previously established cohort of Hanzhong Adolescent Hypertension Study wer...
Source: Disease Markers - July 31, 2020 Category: Laboratory Medicine Tags: Dis Markers Source Type: research