Determination of HbA2 by quantitative bottom-up proteomics and isotope dilution mass spectrometry
Publication date: December 2018Source: Clinica Chimica Acta, Volume 487Author(s): Cristian Gabriel Arsene, Patricia Kaiser, Renata Paleari, André Henrion, Michael Spannagl, Andrea MoscaAbstractBackgroundPoor comparability between laboratories is often observed in the measurement of HbA2. A measurement procedure of higher metrological order is needed for value assignment to a reference material that shall be used as primary calibrator.MethodA reference measurement procedure has been developed based on isotope dilution mass spectrometry (IDMS). The α- and δ- subunits are quantified by signature peptides re...
Source: Clinica Chimica Acta - October 23, 2018 Category: Laboratory Medicine Source Type: research

Whole-exome sequencing for the genetic diagnosis of congenital red blood cell membrane disorders in Taiwan
Conclusions: Our data demonstrated that WES is an efficient tool for determining genetic etiologies of RBC membrane disorders and can facilitate accurate diagnosis and genetic counseling. Additional studies should be conducted on larger cohorts to investigate the distribution of gene mutations in patients with RBC membrane disorders in Taiwan. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 20, 2018 Category: Laboratory Medicine Source Type: research

Selenium levels in whole blood - The borderline low analysis
Publication date: December 2018Source: Clinica Chimica Acta, Volume 487Author(s): Krishnakumar S., Sandhya Iyer, Prachi Sinkar, Caesar SenguptaAbstractObjectivesSelenium is a very important micronutrient. Food is the major source for selenium uptake and the average blood levels is seen to be highly affected by dietary habits as selenium levels in food naturally depend on the kind of soil they are grown upon and in processed foods its levels depend on fortification. Deficiency of this vital nutriment needs to be assessed to understand deficiency prevalence.Design and methodsA total number of 529,461 subjects were tested for...
Source: Clinica Chimica Acta - October 19, 2018 Category: Laboratory Medicine Source Type: research

Utility of estimated glomerular filtration rate using cystatin C and its interpretation in patients with rheumatoid arthritis under glucocorticoid therapy
Publication date: December 2018Source: Clinica Chimica Acta, Volume 487Author(s): Yukiko Nozawa, Hiroe Sato, Ayako Wakamatsu, Daisuke Kobayashi, Takeshi Nakatsue, Yoko Wada, Takeshi Kuroda, Junichiro James Kazama, Yoshiki Suzuki, Masaaki Nakano, Ichiei NaritaAbstractBackgroundPatients with rheumatoid arthritis (RA) often have reduced muscle mass. Estimated glomerular filtration ratio using the serum cystatin C concentration (eGFRcys) is more accurate than eGFR using the serum creatinine (eGFRcreat) because cystatin C is not influenced by muscle mass, but glucocorticoid therapy may affect serum cystatin C concentration.Meth...
Source: Clinica Chimica Acta - October 19, 2018 Category: Laboratory Medicine Source Type: research

Pseudohyperphosphatemia in a patient with incidentally identified progression of smoldering myeloma
ConclusionsPrompt and appropriate laboratory investigations ensure correct diagnosis of pseudohyperphosphatemia and help clinicians properly manage patients. To our knowledge, this patient is the first reported case of pseudohyperphosphatemia in patients with progression from SMM to MM. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 19, 2018 Category: Laboratory Medicine Source Type: research

Effect of hemolysis, icterus, and lipemia on three acetaminophen assays: Potential medical consequences of false positive results
ConclusionHemolysis and icterus had insignificant interference on the Syva EMIT® and the DRI® assays for the analysis of acetaminophen, but significant interference effect on the Roche assay. On the other hand lipemia interfered less markedly with the Roche assay. The effect of hemolysis, icterus and lipemia should always be considered. Cautions are warranted when interpreting results for the potential false positive results in the presence of hemolysis and icterus at the concentrations evaluated in this study. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 18, 2018 Category: Laboratory Medicine Source Type: research

Characterization of PLAC® tests in the quantization of lipoprotein associated phospholipase A2 for assessment of cardiovascular diseases
ConclusionsBoth PLAC® mass and PLAC® activity tests are effective but the CHAPS modified PLAC® mass test has no or less utility in the risk assessment of CVD. The ratio of Lp-PLA2 by either PLAC® mass or PLAC® activity over ApoA1 or (Apo A1 + Apo B) synergistically enhance the risk assessment power for ischemic stroke. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 11, 2018 Category: Laboratory Medicine Source Type: research

Performance evaluation of HbA1c measurement systems with sigma metric for 1066 laboratories in China
ConclusionsThe analytical quality of HbA1c in most laboratories need improving. Laboratories should pay more attention on the performance of HbA1c, and EQA organizers in China should improve evaluation criteria and push standardization work for HbA1c. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 10, 2018 Category: Laboratory Medicine Source Type: research

A rare STAP1 mutation incompletely associated with familial hypercholesterolemia
We describe the case of a 56-year-old male patient found to have hypercholesterolemia at age 34, but who did not continue follow-up nor received treatment with lipid-lowering drugs. At age 55 he suffered a myocardial infarction. A systematic NGS analysis did not show point mutations in the LDLR, APOB, LDLRAP1, or PCSK9 genes, nor large rearrangements of the LDLR gene, but revealed the heterozygous missense variant rs199787258 of STAP1 (c.526C > T; p.Pro176Ser). This variant was also found in heterozygosis in the two siblings of the index case, who also had hypercholesterolemia, but did not cosegregate in his progeny...
Source: Clinica Chimica Acta - October 9, 2018 Category: Laboratory Medicine Source Type: research

Apelin/APJ system: A novel promising target for anti-aging intervention
Publication date: Available online 5 October 2018Source: Clinica Chimica ActaAuthor(s): Qionglin Zhou, Linxi Chen, Mingzhu Tang, Yu Guo, Lanfang LiAbstractApelin, an endogenous ligand for the G protein-coupled receptor APJ, is widely expressed in various organs. Recent research has indicated that the Apelin/APJ system plays an important role in aging. Apelin and APJ receptor expression are down-regulated with increasing age. In murine models, Apelin and APJ knockouts exhibit accelerated senescence whereas Apelin-restoration results in enhanced vigor and rejuvenated behavioral and circadian phenotypes. Furthermore, aged Ape...
Source: Clinica Chimica Acta - October 6, 2018 Category: Laboratory Medicine Source Type: research

Comprehensive assessment of biotin interference in immunoassays
ConclusionsBiotin can interfere in immunoassays and result in aberrant test results. Clinicians should use caution in interpreting abnormal results in patients who ingest biotin. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 6, 2018 Category: Laboratory Medicine Source Type: research

Evaluation of the Siemens ADVIA Centaur high-sensitivity cardiac troponin I assay in serum
Publication date: Available online 6 October 2018Source: Clinica Chimica ActaAuthor(s): Peter A. Kavsak, Andrew Worster, Stephen A. Hill, Allan S. JaffeAbstractBackgroundInternational recommendations on high-sensitivity cardiac troponin (hs-cTn) testing recommend that laboratories select only one sample type for testing. We eluated the Siemens ADVIA Centaur hs-cTnI assay in serum and thereby provide information on imprecision, long-term storage stability, freeze-thaw stability, method comparison to other hs-cTnI assays, and clinical performance.MethodsPatients with chest pain onset
Source: Clinica Chimica Acta - October 6, 2018 Category: Laboratory Medicine Source Type: research

Quality specifications and their daily application to evaluate the accuracy of reference measurements for serum concentrations of 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2
ConclusionsUsing zeta-scores and experimentally derived imprecision, we developed a straightforward approach to assess the acceptability of individual 25(OH)D reference measurements, providing also much-needed practical accuracy specifications for 25(OH)D2. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 6, 2018 Category: Laboratory Medicine Source Type: research

Targeted exome sequencing identified a novel mutation hotspot and a deletion in Chinese primary hypertrophic osteoarthropathy patients
ConclusionsIn the present study, homozygous or compound heterozygous HPGD mutations were identified in seven Chinese pediatric patients, suggesting an autosomal recessive inheritance. The c.310_311delCT mutation and the splicing site mutation c.324 + 5G > A were likely to be mutational hotspots in Chinese PHO patients. For the first time, a structural variation of the HPGD gene was reported. Homozygous, compound heterozygous mutations or structural variation identified in the HPGD gene proposed that targeted exome sequencing may be a preferable method for pediatric PHO diagnosis and mutation analysis. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 6, 2018 Category: Laboratory Medicine Source Type: research

Is capillary zone electrophoresis a suitable method for estimating serum albumin: A comparison of four methods
ConclusionCompared to the nephelometry, turbidimetry and bromocresol purple methods, the capillary zone electrophoresis method tends to overestimate human serum albumin concentrations for levels below 30 g/L. This discrepancy could lead to an overestimation of the nutritional status, an inappropriate scoring of the disease and a delay in nutritional treatment. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 6, 2018 Category: Laboratory Medicine Source Type: research

Establishment of a protein biochip to detect serum IgG antibodies against IL-2 and soluble CD25 in hemophagocytic lymphohistiocytosis
ConclusionsWe addressed and confirmed the involvement of serum IgG antibodies against IL-2 and sCD25 as well as Ag-Ab complex of sCD25 in HLH patients. Therefore, this biochip platform would offer a new technological substitution for clinical serological diagnosis of HLH. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 6, 2018 Category: Laboratory Medicine Source Type: research

An ELISA for quantifying GPIHBP1 autoantibodies and making a diagnosis of the GPIHBP1 autoantibody syndrome
ConclusionWe developed a sensitive and specific ELISA for detecting GPIHBP1 autoantibodies in human serum and plasma; this assay will make it possible to rapidly diagnose the GPIHBP1 autoantibody syndrome. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 4, 2018 Category: Laboratory Medicine Source Type: research

Microscopic examination of urine sediment: Phase contrast versus bright field
ConclusionSeveral papers and monographs, published since the early 1950s, have demonstrated the advantages of PCM over BFM for U-sed examination. In spite of this, PCM is not as widely used as it should be. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 4, 2018 Category: Laboratory Medicine Source Type: research

Discovery of the Long Interspersed Nuclear Element-1 activation product [Open Reading Frame-1 (ORF1) protein] in human blood
In conclusion, we have developed a competitive ELISA and discovered the presence of iORF1 in serum, which could be used to advance future studies involving ORF1 measurement from blood. In addition, iORF1 may be a complement with the PSA screen to better detect prostate cancer. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 4, 2018 Category: Laboratory Medicine Source Type: research

Long non-coding RNA HOXD-AS1 in cancer
In conclusion, HOXD-AS1 may be considered as a promising diagnostic/prognostic biomarker or a novel therapeutic target for cancers. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 4, 2018 Category: Laboratory Medicine Source Type: research

Contribution of obesity as an effect regulator to an association between serum leptin and incident metabolic syndrome
ConclusionsObesity is an effect regulator, which can predict an association between increased serum leptin level and the incidence of MetS in lean subjects. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 4, 2018 Category: Laboratory Medicine Source Type: research

C-src tyrosine kinase gene rs1378942 polymorphism and hypertension in Asians: Review and meta-analysis
ConclusionThe meta-analysis demonstrates that the C allele of the CSK rs1378942 is associated with higher SBP and DBP levels in East Asians. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 4, 2018 Category: Laboratory Medicine Source Type: research

Challenges facing nanotoxicology and nanomedicine due to cellular diversity
Publication date: Available online 3 October 2018Source: Clinica Chimica ActaAuthor(s): Mohd Javed Akhtar, Maqusood Ahamed, Hisham A. AlhadlaqAbstractThis review examines the interaction of nanomaterials (NMs) with cells from the perspective of major cellular differentiations. The structure and composition of cells reflect their role and function in a particular organ or environment. The normal differentiated-state and diseased cells may respond to NMs very differently. This review progresses with due care on nanotoxicology while emphasizing the potential of NMs in treating stress-associated disorders, including cancer and...
Source: Clinica Chimica Acta - October 4, 2018 Category: Laboratory Medicine Source Type: research

Sensitivity and colour intensity enhancement in lateral flow immunoassay tests by adjustment of test line position
Publication date: Available online 3 October 2018Source: Clinica Chimica ActaAuthor(s): Sasan Asiaei, Mostafa Rabbani Bidgoli, Ali ZadehKafi, Narges Saderi, Majid SiavashiAbstractIn this article, we have enhanced the sensitivity and limit of detection of lateral flow immunoassay tests, exemplified for the detection of human chorionic gonadotropin (pregnancy) through determination of proper test line position. Test line position affects the flow velocity and concentration profile of analytes at the test line, which itself impacts the reaction rate and thus the colour intensity. The flow velocity was precisely modelled, both...
Source: Clinica Chimica Acta - October 4, 2018 Category: Laboratory Medicine Source Type: research

Aldehyde dehydrogenase II rs671 polymorphism in essential hypertension
Publication date: December 2018Source: Clinica Chimica Acta, Volume 487Author(s): Yuncao Fan, Zhongying Chen, Tingting Ye, Wenhui Lin, Qizeng Wang, Bin Lin (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 4, 2018 Category: Laboratory Medicine Source Type: research

Serum ST2 as a potential prognostic biomarker for traumatic brain injury
ConclusionIncreased serum sST2 concentrations are significantly related to inflammation, severity and prognosis, substantialized ST2 as a potential prognostic biomarker for TBI. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 4, 2018 Category: Laboratory Medicine Source Type: research

High-throughput genotyping of GC (vitamin D-binding protein) by melting analysis with locked nucleic acid-incorporating dual hybridization probe for improving mismatch discrimination
Publication date: December 2018Source: Clinica Chimica Acta, Volume 487Author(s): Takayuki Ishige, Mamoru Satoh, Sakae Itoga, Motoi Nishimura, Kazuyuki Matsushita, Fumio Nomura (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - October 4, 2018 Category: Laboratory Medicine Source Type: research

Clinical, biochemical and genetic analysis of Chinese patients with isobutyryl-CoA dehydrogenase deficiency
Publication date: December 2018Source: Clinica Chimica Acta, Volume 487Author(s): Yiming Lin, Weilin Peng, Mengyi Jiang, Chunmei Lin, Weihua Lin, Zhenzhu Zheng, Min Li, Qingliu FuAbstractIsobutyryl-CoA dehydrogenase deficiency (IBDHD) is a rare autosomal recessive metabolic disorder related to valine catabolism and results from variants in ACAD8. Here, we present the clinical, biochemical, and genotypes of seven patients with IBDHD in China for the first time. Five patients remained asymptomatic during follow-up, whereas one juvenile had speech delay and one newborn exhibited clinical symptoms. All patients showed remarkab...
Source: Clinica Chimica Acta - October 4, 2018 Category: Laboratory Medicine Source Type: research

Detection of WNT2B, WIF1 and β-catenin expression in preeclampsia by placenta tissue microarray
Publication date: Available online 21 September 2018Source: Clinica Chimica ActaAuthor(s): Linlin Zhang, Wei Li, Wanyu Song, Yunwei Ran, Yangyang Yuan, Liting Jia, Lisha Liu, Ying Li, Shihong Cui, Zhan Zhang (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - September 22, 2018 Category: Laboratory Medicine Source Type: research

Serum levels of trace elements in children born after assisted reproductive technology
This study was designed to investigate whether ART children have differences in the levels of trace elements compared with naturally conceived children.MethodsThis study included those ART children and controls aged 1 to 12 years assessed with a follow-up protocol. Serum levels of the trace elements zinc, copper, iron, calcium, magnesium and lead were determined and analyzed.ResultsThere were no significant differences in age, gender or body weight between the ART and control groups. There were no significant differences in the rates of deficiency or excess of trace elements between the two groups. Serum lead levels in c...
Source: Clinica Chimica Acta - September 22, 2018 Category: Laboratory Medicine Source Type: research

Editorial board member, Aims and Scope, Publication Info.
Publication date: November 2018Source: Clinica Chimica Acta, Volume 486Author(s): (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - September 22, 2018 Category: Laboratory Medicine Source Type: research

Evaluation of the new Sebia free light chain assay using the AP22 ELITE instrument
ConclusionsSebia put forward new technology that automatically measures free light chains. This technique is suitable for routine use; however, the results cannot be used interchangeably with Freelite kits. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - September 20, 2018 Category: Laboratory Medicine Source Type: research

Methodological development and biological observations of cell free DNA with a simple direct fluorescent assay in colorectal cancer
Publication date: Available online 18 September 2018Source: Clinica Chimica ActaAuthor(s): A.K. Boysen, B.S. Sørensen, A.C. Lefevre, R. Abrantes, J.S. Johansen, B.V. Jensen, J.V. Schou, F.O. Larsen, D. Nielsen, H. Taflin, B. Gustavson, Y. Wettergren, B.S. Sorensen, A.H. Ree, S. Dueland, N. Pallisgaard, K.L. SpindlerAbstractBackgroundCell free DNA (cfDNA) has shown promising utility as prognostic biomarker for patients with colorectal cancer (CRC), with an ongoing need to optimize and validate the laboratory methodology. Here, we report our optimization and validation of a direct fluorescent assay and display the pot...
Source: Clinica Chimica Acta - September 19, 2018 Category: Laboratory Medicine Source Type: research

Association of serum metabolites with impaired fasting glucose/diabetes and traditional risk factors for metabolic disease in Chinese adults
Publication date: Available online 17 September 2018Source: Clinica Chimica ActaAuthor(s): Siming Wang, Xue Yu, Wenduo Zhang, Fusui Ji, Mo Wang, Ruiyue Yang, Hongxia Li, Wenxiang Chen, Jun DongAbstractBackgroundHyperglycemia has become a major health problem worldwide. We investigated the associations of serum metabolite levels with hyperglycemia (impaired fasting glucose/diabetes) and traditional risk factors for metabolic disease.MethodsA total of The 563 Chinese adults were categorized into hyperglycemia and control groups. Associations of serum metabolites, including branched-chain amino acids (BCAAs), aromatic amino a...
Source: Clinica Chimica Acta - September 18, 2018 Category: Laboratory Medicine Source Type: research

Measuring gradients in body fluids – A tool for elucidating physiological processes, diagnosis and treatment of disease
Publication date: Available online 23 August 2018Source: Clinica Chimica ActaAuthor(s): Hanne Haslene-Hox (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - September 16, 2018 Category: Laboratory Medicine Source Type: research

Metabolites related to eGFR: Evaluation of candidate molecules for GFR estimation using untargeted metabolomics
ConclusionsOur results demonstrate metabolites that are potential biomarkers of renal function: d-threitol, myo-inositol, 4-deoxierythronic acid, galacturonic acid and pseudouridine. Further investigation is needed to determine their performance against otherwise gold-standard methods, most notably among those with normal eGFR. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - September 16, 2018 Category: Laboratory Medicine Source Type: research

A simple screening method for the diagnosis of chronic myeloid leukemia using the parameters of a complete blood count and differentials
ConclusionsThe absolute basophil count is effective for screening for CML. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - September 16, 2018 Category: Laboratory Medicine Source Type: research

Exploring collagen remodeling and regulation as prognosis biomarkers in stable heart failure
Publication date: Available online 1 September 2018Source: Clinica Chimica ActaAuthor(s): Anne Marie Dupuy, Nils Kuster, Corentin Curinier, Fabien Huet, Maelle Plawecki, Kamila Solecki, François Roubille, Jean Paul CristolAbstractWe assessed the predictive ability of circulating biomarkers involved in collagen synthesis (procollagen type I N-terminal propeptide [PINP], and procollagen type III N-terminal propeptide [PIIINP], collagen degradation (c-terminal telopeptide of collagen type I [CTx] and mediators of cardiac fibrosis (Galectin-3 and soluble suppression of tumorigenicity 2 protein or sST2) as prognosis mark...
Source: Clinica Chimica Acta - September 16, 2018 Category: Laboratory Medicine Source Type: research

Identification of an inherited pathogenic DNAJC12 variant in a patient with hyperphenylalalinemia
Publication date: Available online 1 September 2018Source: Clinica Chimica ActaAuthor(s): Yi Feng, Sichi Liu, Chengfang Tang, Xiang Jiang, Fang Tang, Bei Li, Xuefang Jia, Qianyu Chen, Jilian Liu, Yonglan HuangAbstractHyperphenylalaninemia (HPA), an abnormal condition of phenylalanine metabolism, was recently reported to be caused by DNAJC12 mutations. As the heat shock co-chaperone, DNAJC12 prevents the aggregation of misfolded or aggregation-prone proteins and maintain the correct assembly and degradation. Here, we report a patient with unexplained HPA detected by newborn screening. Differential diagnoses of pterin profil...
Source: Clinica Chimica Acta - September 16, 2018 Category: Laboratory Medicine Source Type: research

Haptoglobin phenotype is a critical factor in the use of fucosylated haptoglobin for pancreatic cancer diagnosis
In this study, we measured Fuc-Hpt using a lectin-antibody enzyme-linked immunosorbent assay (ELISA) or a novel ELISA system that used a glycan antibody for Fuc-Hpt. Hpt is known to be divided into three phenotypes (Hpt1–1, Hpt2–1, and Hpt2–2), depending on its genetic background. Normal levels of serum Hpt are different in each Hpt phenotype and these phenotypes are associated with the incidence of several human diseases. Here, we investigated how Hpt phenotype affected measurements of Fuc-Hpt, using two kinds of ELISA. Interestingly, we found that serum Fuc-Hpt levels were dramatically lower in the Hpt1...
Source: Clinica Chimica Acta - September 16, 2018 Category: Laboratory Medicine Source Type: research

Real time detection of glutathionse in chemotherapy squamous-cell carcinoma cells of a fluorescent probe
Publication date: Available online 5 September 2018Source: Clinica Chimica ActaAuthor(s): Mengxing Qi, Meng Ma, Shengyun Huang, Dongsheng Zhang, Hongchen LiuAbstractGlutathione (GSH) plays a key role in protecting damage induced by radiation and chemotherapy drugs. Current methods, which quantify GSH level changes by using cellular extraction or being based on redox homeostasis results, are unable to directly measure real time GSH level in live cells. In this article, we utilized a newly designed reversible fluorescent probe to measure GSH in living cells. The image of probe 1 can reveal intracellular GSH location and GSH ...
Source: Clinica Chimica Acta - September 16, 2018 Category: Laboratory Medicine Source Type: research

The potential of exosomes derived from colorectal cancer as a biomarker
Publication date: Available online 6 September 2018Source: Clinica Chimica ActaAuthor(s): Yang Xiao, Yan Li, Yuhui Yuan, Bing Liu, Shimeng Pan, Qianqian Liu, Xia Qi, Huimin Zhou, Weijie Dong, Li JiaAbstractColorectal cancer (CRC) is one of the most frequent causes of cancer death. The diagnosis and treatment for metastatic CRC and patients with drug resistance remains poor. Cancer cells characteristically produce and release exosomes, which act as a new signal pattern in the occurrence and development of tumors. Circulating exosomes constitute promising biomarkers for early diagnosis of cancer patients. However, the potent...
Source: Clinica Chimica Acta - September 16, 2018 Category: Laboratory Medicine Source Type: research

Immunoluminometric assay for copeptin measurement in cerebrospinal fluid: Technical aspects and pilot study
ConclusionsThis assay provides to best of our knowledge for the first time initial ranges values of CSF copeptin for patients without acute neurological disease and in patients with aSAH. Thus, it opens new doors to develop further calculations and relationships between diseases biomarker and outcome prediction. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - September 16, 2018 Category: Laboratory Medicine Source Type: research

RAS mutations in acute myeloid leukaemia patients: A review and meta-analysis
In conclusion, this study revealed that RAS mutations did not influence the over survival for adults with AML. However, NRAS mutations may be a key prognostic marker related with poor survival for children with AML. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - September 16, 2018 Category: Laboratory Medicine Source Type: research

Persistently elevated plasma heart-type fatty acid binding protein concentration is related with poor outcome in acute decompensated heart failure patients
Publication date: Available online 6 September 2018Source: Clinica Chimica ActaAuthor(s): Ewelina Kazimierczyk, Remigiusz Kazimierczyk, Ewa Harasim-Symbor, Karol Kaminski, Bozena Sobkowicz, Adrian Chabowski, Agnieszka TycinskaAbstractBackgroundThe aim of the study was to determine clinical and prognostic role of repeated heart-type fatty acid binding protein (hFABP) measurements in acute decompensated HF (ADHF) patients.MethodsIn seventy-seven ADHF patients (III and IV NYHA class, mean age 70 ± 12.7 years, mean left ventricle ejection fraction [LVEF] 29.73 ± 13.3%) plasma hFABPs concentrations (SunR...
Source: Clinica Chimica Acta - September 16, 2018 Category: Laboratory Medicine Source Type: research

Potential role for Rv2026c- and Rv2421c- specific antibody responses in diagnosing active tuberculosis
Publication date: Available online 6 September 2018Source: Clinica Chimica ActaAuthor(s): Yanqing Chen, Shuhui Cao, Yi Liu, Xuxia Zhang, Wei Wang, Chuanyou LiAbstractThe current diagnostic methods for tuberculosis (TB) have several limitations. Although commercial serological tests based on antibody detection are available, their variable accuracies limit their roles in the clinic. The aim of this study was to discover the improved biomarkers for TB disease by investigating the serum profiles of IgG and IgM antibodies against nearly all Mycobacterium tuberculosis (MTB) antigens in 36 active TB patients and 18 healthy contr...
Source: Clinica Chimica Acta - September 16, 2018 Category: Laboratory Medicine Source Type: research

Dyslipidemias in clinical practice
Publication date: Available online 7 September 2018Source: Clinica Chimica ActaAuthor(s): Manuel Castro Cabezas, Benjamin Burggraaf, Boudewijn KlopAbstractMost dyslipidemic conditions have been linked to an increased risk of cardiovascular disease. Over the past few years major advances have been made regarding the genetic and metabolic basis of dyslipidemias. Detailed characterization of the genetic basis of familial lipid disorders and knowledge concerning the effects of environmental factors on the expression of dyslipidemias have increased substantially, contributing to a better diagnosis in individual patients. In add...
Source: Clinica Chimica Acta - September 16, 2018 Category: Laboratory Medicine Source Type: research

Metabolic disorders and inflammation are associated with familial combined hyperlipemia
The objective of this study was to evaluate the presence of alterations of hydrocarbonated metabolism and lipid profile together with inflammatory and adhesion molecules in subjects with FCH compared to controls.Methods75 HFC patients and 75 healthy individuals were studied. Glucose, insulin, HOMA-IR index and lipid parameters, in addition to anti-oxidized LDL antibodies (Anti ox-LDL), small and dense LDL (sdLDL) and HDL subfractions, proinflammatory cytokines and adhesion molecules were measured.ResultsFCH patients showed higher levels of hydrocarbonated metabolism parameters, total cholesterol, triglycerides, LDLc, Apoli...
Source: Clinica Chimica Acta - September 16, 2018 Category: Laboratory Medicine Source Type: research

Prevalence and influencing factors of the high nil-control spot count in T-SPOT.TB: A matched case-control study
ConclusionsHigh nil responses in T-SPOT.TB assays are quite rare. Male gender and Behcet's disease are suggested as independent risk factors, while recent excessive use of corticosteroids and hypoalbuminemia seem to be independent protective factors. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - September 16, 2018 Category: Laboratory Medicine Source Type: research

Neutrophil-lymphocyte ratio is associated with all-cause mortality among critically ill patients with acute kidney injury
ConclusionsOur analysis indicates that a higher level of NLR is associated with increased risk of 30-day and 90-day mortality in AKI patients. The similar upward trend is not detected in analysis of in-hospital mortality. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - September 16, 2018 Category: Laboratory Medicine Source Type: research