Association of serum uric acid with bone mineral density and clinical fractures in Chinese type 2 diabetes mellitus patients: A cross-sectional study
ConclusionsLower serum UA concentrations may be associatedwith lower BMD values and higher prevalence of clinical fractures independent of potential confounders except for BMD values at each site. These findings need to be confirmed by further prospective studies. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 20, 2018 Category: Laboratory Medicine Source Type: research

A rapid ultra-performance LC-MS/MS assay for determination of serum unbound fraction of voriconazole in cancer patients
Publication date: November 2018Source: Clinica Chimica Acta, Volume 486Author(s): Jieli Li, Jing Ma, Elizabeth A. Wagar, Dong Liang, Qing H. MengAbstractBackgroundVoriconazole (VOR), an antifungal agent, is clinically monitored to guide therapeutic dosing and avoid toxicity. It is believed that measurement of serum unbound VOR provides more accurate information, especially in hypoalbuminemia patients. We developed and validated an accurate, simple and fast test with ultrafiltration and ultra-performance liquid chromatography (UPLC)-tandem mass spectrometry (MS/MS) to measure unbound VOR in human serum.MethodsThe Agilent UP...
Source: Clinica Chimica Acta - July 20, 2018 Category: Laboratory Medicine Source Type: research

Relationship between resting heart rate and metabolic risk factors in breast cancer patients
Publication date: Available online 18 July 2018Source: Clinica Chimica ActaAuthor(s): Mi Kyung Lee, Dong Hoon Lee, Seho Park, Seung Il Kim, Justin Y. JeonAbstractBackgroundHigher resting heart rate (RHR) was associated with poor prognosis in breast cancer survivors, but the mechanism underlying such association has not been fully studied. We investigated the association between RHR and metabolic risk factors in stage I-III breast cancer survivors.MethodsAmong 11,013 women diagnosed with breast cancer between 2005 and 2013 at the Severance hospital in Seoul, Korea, a total of 4980 patients met our inclusion criteria for the...
Source: Clinica Chimica Acta - July 19, 2018 Category: Laboratory Medicine Source Type: research

Capillary Electrophoresis (CE) vs. HPLC in the determination of asialo-Tf, a crucial marker for the reliable interpretation of questioned CDT increases
ConclusionsThe present work shows a superior performance of CE vs. HPLC for the determination of asialo-Tf and the importance of this CDT component to avoid misinterpretation of non-alcohol related CDT increases. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 19, 2018 Category: Laboratory Medicine Source Type: research

Circulating circular RNA hsa_circ_0001785 acts as a diagnostic biomarker for breast cancer detection
Publication date: Available online 16 October 2017Source: Clinica Chimica ActaAuthor(s): Wei-Bing Yin, Ming-Guang Yan, Xiao Fang, Jian-Jun Guo, Wei Xiong, Ruo-Peng ZhangAbstractBackgroundCircular RNAs (circRNAs) are a class of non-coding RNAs (ncRNAs) and characterized by covalently closed loop without 5′ and 3′ end. Recently, the diagnostic value of circRNAs has received more and more attention. However, the in-depth study about circRNAs diagnosis in breast cancer is still rarely reported. In present study, we try to investigate the circRNAs expression profiles in breast cancer peripheral blood and discover va...
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Real-time PCR-based method for the rapid detection of extended RAS mutations using bridged nucleic acids in colorectal cancer
Publication date: Available online 27 October 2017Source: Clinica Chimica ActaAuthor(s): Takao Iida, Yukie Mizuno, Yasuharu KaizakiAbstractMutations in RAS and BRAF are predictors of the efficacy of anti-epidermal growth factor receptor (EGFR) therapy in patients with metastatic colorectal cancer (mCRC). Therefore, simple, rapid, cost-effective methods to detect these mutations in the clinical setting are greatly needed. In the present study, we evaluated BNA Real-time PCR Mutation Detection Kit Extended RAS (BNA Real-time PCR), a real-time PCR method that uses bridged nucleic acid clamping technology to rapidly detect mut...
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Falsely high sirolimus concentrations due to everolimus cross-reactivity in the Siemens sirolimus immunoassay: Corrective actions implemented
Publication date: Available online 29 October 2017Source: Clinica Chimica ActaAuthor(s): Raffick Bowen, Ranilo Rieta, Rohan Joshi, Roy C. Lee (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Comparison of glutathione peroxidase-3 protein expression and enzyme bioactivity in normal subjects and patients with sepsis
The objectives of this study are (1) to compare the GPx-3 protein concentrations and GPx-3 bioactivity in normal healthy subjects and septic patients, and (2) to evaluate the relationship between GPx-3 bioactivity and its protein concentration.MethodsSerum samples were collected from 50 normal healthy subjects and 70 septic patients. The reliable bioanalytical methods for GPx-3 protein concentration and bioactivity in human serum were developed and validated. Analyses of GPx-3 bioactivity and GPx-3 protein concentration were then performed.ResultsGeometric mean GPx-3 bioactivity was 78.13 U/l for patients with sepsis,...
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Metabolomic analysis—Addressing NMR and LC-MS related problems in human feces sample preparation
In this study different pre-analytical parameters and factors were investigated i.e. water content, different extraction solvents, influence of freeze-drying and homogenization, ratios of sample weight to extraction solvent, and their respective impact on metabolite profiles acquired by NMR and LC-MS.The results indicate that profiles are strongly biased by selection of extraction solvent or drying of samples, which causes different metabolites to be lost, under- or overstated. Additionally signal intensity and reproducibility of the measurement were found to be strongly dependent on sample pre-treatment steps: freeze-dryi...
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Circulating asprosin concentrations are increased in type 2 diabetes mellitus and independently associated with fasting glucose and triglyceride
Publication date: Available online 2 November 2017Source: Clinica Chimica ActaAuthor(s): Lei Zhang, Chao Chen, Nan Zhou, Yuming Fu, Xingbo ChengAbstractBackgroundAsprosin has been identified as a novel hormone enriched in white adipose tissue and is pathologically increased in insulin-resistant mice and humans. However, information regarding the role of asprosin in type 2 diabetes mellitus (T2DM) remains unavailable. Via conducting a hospital-based study, we purposed to ascertain the potential relationship between circulating asprosin concentrations and T2DM.MethodsThe study recruited 84 adults with T2DM and 86 controls wi...
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Critical review of 2016 ACC guidelines on therapies for cholesterol lowering with reference to laboratory testing
ConclusionsThe 2016 guidelines appear to eliminate most of the flaws in the prior 2013 guidelines that replaced the ATP. They do not seem to rely on randomized control studies alone but the totality of all of the evidence. The new guidelines still fail to identify some persons with characteristics of metabolic syndrome that may be of increased risk for ASCVD although they address some of the problems in treating this group. The guidelines identify LDL cholesterol and nonHDL cholesterol target concentrations that are important for laboratory professionals since they should be defined on reports and are important for consult...
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Increased levels of interleukin-22 in thoracic aorta and plasma from patients with acute thoracic aortic dissection
Publication date: Available online 2 November 2017Source: Clinica Chimica ActaAuthor(s): Jing Ye, Menglong Wang, Huimin Jiang, Qingwei Ji, Ying Huang, Jianfang Liu, Tao Zeng, Yao Xu, Zhen Wang, Yingzhong Lin, Jun WanAbstractBackgroundInterleukin (IL)-22 plays important roles in the development of arterial disease, including atherosclerosis and hypertension. However, the relationship between IL-22 and acute thoracic aortic dissection (TAD) remains unknown.MethodsBlood samples were collected from patients with chest pain who underwent computed tomography angiography of the thoracic aorta but had no known preoperative diagnos...
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Fibroblast growth factor 21 in chronic kidney disease
Publication date: Available online 3 November 2017Source: Clinica Chimica ActaAuthor(s): Sahapab Anuwatmatee, Shudi Tang, Ben J. Wu, Kerry-Anne Rye, Kwok Leung OngAbstractThe association between fibroblast growth factor 21 (FGF21) and kidney function has been extensively studied in recent years in both animal and human studies. However, the exact functional role of FGF21 in the kidney remains unclear. Previous animal studies have shown that administration of FGF21 ameliorates kidney function, morphological glomerular abnormalities, dyslipidemia, hyperglycemia, insulin resistance, oxidative stress and obesity. In human stud...
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Heat shock protein 27 acts as a predictor of prognosis in chronic heart failure patients: Methodological issues
Publication date: Available online 21 November 2017Source: Clinica Chimica ActaAuthor(s): Jie Weng, He Wu, Zhiyi Wang (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

MIR-10b IS inversely correlated with higher tumor grade in osteosarcoma
Publication date: Available online 24 November 2017Source: Clinica Chimica ActaAuthor(s): Gabriela Molinari Roberto, Edgard Eduard Engel, Luiz Gonzaga Tone, María Sol Brassesco (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Hypoxia-induced and HIF1α-VEGF-mediated tight junction dysfunction in choriocarcinoma cells: Implications for preeclampsia
ConclusionThese data demonstrate that trophoblast cells undergo significant changes in TJ protein expression under hypoxic conditions and highlight the potential significance of the HIF1α-VEGF axis in the regulation of TJ structure and function in the preeclamptic placenta. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Recovery from childhood community-acquired pneumonia in a developing country: Prognostic value of serum procalcitonin
Publication date: Available online 16 December 2017Source: Clinica Chimica ActaAuthor(s): Taiane S. Fonseca, Ângela G. Vasconcellos, Dominique Gendrel, Olli Ruuskanen, Cristiana M. Nascimento-CarvalhoAbstractBackgroundChildhood community-acquired pneumonia is a common and potentially life-threatening illness in developing countries. We assessed the prognostic value of serum procalcitonin level upon admission on clinical response to antibiotic treatment.MethodsOut of 89 patients, the median (IQR) age was 19(12–29) months and 60% were boys. Viral (49.5%), typical bacterial (38%) and atypical bacterial (12.5%) inf...
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Gene expression patterns associated with human placental trophoblast differentiation
Publication date: Available online 10 January 2018Source: Clinica Chimica ActaAuthor(s): Shi-Wen Jiang, Wei Zhou, Jianhao Wang, Lauren M. Little, Lynn Leaphart, Jacob Jay, Eseosaserea Igbinigie, Haibin Chen, Jinping LiAbstractCell fusion is a hallmark of placental trophoblast cell differentiation and the mature syncytiotrophoblasts play essential roles for fetal-maternal exchange and production of pregnancy-related hormones. Using a well-established in vitro trophoblast differentiation model, we performed a microarray analysis on mRNA expression in trophoblast and syncytiotrophoblast cell cultures. Dramatic changes in gene...
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Effect of Hyperin and Icariin on steroid hormone secretion in rat ovarian granulosa cells
ConclusionsThese results showed that Hyperin and Icariin can promote the secretion of E2 and P through up-regulation of CYP17 and CYP19. Frequently used Chinese herbs like Cuscuta Chinensis Lam and Epimedium Brevicornu maxim, which contain Hyperin and Icariin, could improve the ovarian endocrine function through these effects. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Intraoperative ultrasound-assisted enucleation of residual fibroids following laparoscopic myomectomy
ConclusionsBoth LUS and TVS are beneficial to surgical treatment of fibroids by assisting enucleation of residual fibroids following LM, while LUS is more effective in localizing residual fibroids than TVS. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Optimization and application of an HPLC method for quantification of Inosine-5′-monophosphate dehydrogenase activity as a pharmacodynamic biomarker of mycophenolic acid in Chinese renal transplant patients
ConclusionA bioanalytical assay for IMPDH quantification was optimized and evaluated. The differences in the pharmacodynamics of MPA between Asians and Caucasians may provide some evidence for dosing differences among ethnicities. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Increased glycine levels in cerebrospinal fluid of patients with multiple sclerosis
Publication date: Available online 17 July 2018Source: Clinica Chimica ActaAuthor(s): Monika Ďurfinová, Radka Bartová, Katarína Orešanská, Ladislav Turecký, Ľubica Procházková, Darina Petrleničová, Branislav Líška (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Correlation between hepatitis B core-related antigen and HBV viral load in chronic hepatitis B and hepatocellular carcinoma patients
ConclusionsOur data show that HBcrAg can serve as a biomarker to assess HBV loads in patients and provide a better method for monitoring cccDNA in HCC patients than HBV DNA. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Age and sex differences in the relationship between neutrophil-to-lymphocyte ratio and chronic kidney disease among an adult population in Taiwan
This study investigated the association between systemic inflammation and chronic kidney disease (CKD), and whether this association changes with aging in adults, by using neutrophil-to-lymphocyte ratio (NLR) as an inflammation marker.Materials and methodsA total of 2954 adults (1815 men and 1139 women) who attended a health examination at a medical center in central Taiwan were included for the final cross-sectional analysis.ResultsCompared with participants aged
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Pediatric reference intervals for calculated LDL cholesterol, non-HDL cholesterol, and remnant cholesterol in the healthy CALIPER cohort
Publication date: Available online 17 July 2018Source: Clinica Chimica ActaAuthor(s): V. Higgins, S. Asgari, M.K. Chan, K. AdeliAbstractObjectivesIncreased prevalence of pediatric obesity and associated co-morbidities has heightened the concern for cardiovascular disease (CVD) risk later in life. Although the fasting lipid profile is traditionally used to assess CVD risk, the non-fasting lipid profile may simplify lipid testing and better predict CVD risk. Unfortunately, non-fasting lipid reference values are limited, particularly for children. The Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) h...
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Lymphocyte-to-monocyte ratio in esophageal squamous cell carcinoma prognosis
Publication date: Available online 17 July 2018Source: Clinica Chimica ActaAuthor(s): Gang Hu, Gang Liu, Jian-ying Ma, Ru-jin HuAbstractIntroductionRecently, the prognostic value of the lymphocyte-to-monocyte ratio (LMR) has been widely evaluated in multiple malignancies. However, its prognostic value in patients with esophageal squamous cell carcinoma (ESCC) remains controversial. Herein, we perform a meta-analysis to assess the prognostic value of the preoperative LMR in ESCC.MethodsRelevant studies were systematically retrieved from the online Cochrane, MEDLINE, and EMBASE databases published until March 2018. Data from...
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Circulating lncRNA XLOC_009167 serves as a diagnostic biomarker to predict lung cancer
ConclusionsLncRNA XLOC_009167 could serve as a novel diagnostic biomarker to distinguish lung cancer from benign lung disease and healthy controls. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Serum biomarkers to predict liver fibrosis in hepatitis B
Publication date: November 2018Source: Clinica Chimica Acta, Volume 486Author(s): Hemant Goyal, Guo-Ming Zhang (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

The sample that would not clot
ConclusionThis case displays the importance of genetic testing prior to warfarin dosing and the role antibiotics play in the coagulation cascade. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 18, 2018 Category: Laboratory Medicine Source Type: research

Editorial board member, Aims and Scope, Publication Info.
Publication date: September 2018Source: Clinica Chimica Acta, Volume 484Author(s): (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 15, 2018 Category: Laboratory Medicine Source Type: research

AGR2 diagnostic value in nasopharyngeal carcinoma prognosis
Publication date: September 2018Source: Clinica Chimica Acta, Volume 484Author(s): Yuejin Li, Weiwei Wang, Zheliang Liu, Yuan Jiang, Jinping Lu, Haitao Xie, Faqing TangAbstractBackgroundAnterior Gradient (AGR) 2 concentration increases in the serum of tumor patients, and their diagnostic and prognostic significances were evaluated in some tumors. The previous works showed that AGR2 high express in nasopharyngeal carcinoma (NPC) biopsy tissues. However, whether AGR2 serves as a diagnostic and prognostic marker for NPC remains unclear.Methods42 healthy volunteers, 34 breast cancer patients and 124 NPC patients were enrolled ...
Source: Clinica Chimica Acta - July 15, 2018 Category: Laboratory Medicine Source Type: research

Call for more transparency in manufacturers declarations on serum indices: On behalf of the Working Group for Preanalytical Phase (WG-PRE), European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)
Publication date: September 2018Source: Clinica Chimica Acta, Volume 484Author(s): Alexander von Meyer, Janne Cadamuro, Giuseppe Lippi, Ana-Maria SimundicAbstractThe presence of interfering substances like free hemoglobin, bilirubin or lipids compromises sample quality and potentially affects laboratory analysis and test results. Recently, the use of serum indices for objectively assessing sample quality has become commonplace and many preanalytical platforms, as well as clinical chemistry and coagulation analyzers, are now equipped with this analytical feature. Nevertheless, some important drawbacks remain in this practic...
Source: Clinica Chimica Acta - July 15, 2018 Category: Laboratory Medicine Source Type: research

Validation of a simple, fast liquid chromatography-tandem mass spectrometry method for the simultaneous quantification of 40 antidepressant drugs or their metabolites in plasma
ConclusionThe presented method successfully quantifies 40 compounds of interest. Because of a simple sample clean-up, a relatively short chromatographic run and a wide calibration range this method can be implemented in therapeutic drug monitoring, forensic research and related fields.Graphical abstract (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 13, 2018 Category: Laboratory Medicine Source Type: research

A preliminary investigation of metal element profiles in the serum of patients with bloodstream infections using inductively-coupled plasma mass spectrometry (ICP-MS)
ConclusionWe found differential metal elements in the serum of BSI patients compared with controls, thus providing a basis for the diagnosis, prevention and treatment of BSI from the perspective of metallomics. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 12, 2018 Category: Laboratory Medicine Source Type: research

Reference intervals for serum 1,5-anhydroglucitol in children, adolescents, adults, and pregnant women
Publication date: Available online 11 July 2018Source: Clinica Chimica ActaAuthor(s): Marciane Welter, Kátia C. Boritza, Mauren I. Anghebem-Oliveira, Railson Henneberg, Aline B. Hauser, Fabiane G.M. Rego, Geraldo PichethAbstractBackground1,5-anhydroglucitol (1,5-AG) is a validated marker of short-term glycemic control. We determined the reference intervals of 1,5-AG in different age groups and during pregnancy.MethodsBlood samples were collected from 2303 Euro-Brazilian healthy subjects: 580 children, 496 adolescents, 922 adults matched by age and sex, and 305 pregnant women in four gestational periods. Serum 1,5-AG...
Source: Clinica Chimica Acta - July 11, 2018 Category: Laboratory Medicine Source Type: research

Tumor necrosis factor-α and diabetic retinopathy: Review and meta-analysis
ConclusionThe results this meta-analysis indicated that the level of TNF-α in DR patients was significantly different from that in the healthy controls, so TNF-α represents a candidate biomarker for DR. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 11, 2018 Category: Laboratory Medicine Source Type: research

Whole genome sequencing identifies a de novo 2.1 Mb balanced paracentric inversion disrupting FOXP1 and leading to severe intellectual disability
Publication date: October 2018Source: Clinica Chimica Acta, Volume 485Author(s): M.-L. Vuillaume, B. Cogné, M. Jeanne, A. Boland, D.-C. Ung, D. Quinquis, T. Besnard, J.-F. Deleuze, R. Redon, S. Bézieau, F. Laumonnier, A. ToutainAbstractThe FOXP1 gene, located on chromosome 3p13, encodes the Forkhead-box protein P1, one of the four forkhead transcription factors which repress transcription by forming active homo- and heterodimers and regulate distinct patterns of gene expression crucial for embryogenesis and normal development. FOXP1 mutations, mostly truncating, have been described in patients with mild to mo...
Source: Clinica Chimica Acta - July 11, 2018 Category: Laboratory Medicine Source Type: research

Urinary Syndecan-1 and acute kidney injury after pediatric cardiac surgery
ConclusionUrinary syndecan-1 predicts severe AKI after pediatric cardiac surgery. Moreover, it appears to add capacity to predict severe AKI into a clinical model. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 11, 2018 Category: Laboratory Medicine Source Type: research

Software updates of Tosoh HPLC analyzers can cause and resolve interference of Hb-variants for HbA1c
Publication date: October 2018Source: Clinica Chimica Acta, Volume 485Author(s): Erna Lenters-Westra (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 11, 2018 Category: Laboratory Medicine Source Type: research

Serum bikunin is a biomarker of linkeropathies
Publication date: Available online 30 June 2018Source: Clinica Chimica ActaAuthor(s): Arnaud Bruneel, Johanne Dubail, Charles Roseau, Pierre Prada, Walid Haouari, Céline Huber, Thierry Dupré, Christian Poüs, Valérie Cormier-Daire, Nathalie Seta (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 11, 2018 Category: Laboratory Medicine Source Type: research

Soluble CD14 is associated with the structural failure of bioprostheses
Publication date: Available online 30 June 2018Source: Clinica Chimica ActaAuthor(s): Mohamed J. Nsaibia, Marie-Chloé Boulanger, Rihab Bouchareb, Ghada Mkannez, Abdellaziz Dahou, Erwan Salaun, Yohan Bossé, Marie-Annick Clavel, Benoit J. Arsenault, Philippe Pibarot, Patrick MathieuAbstractIntroductionAortic valve bioprostheses, which do not mandate chronic anticoagulation, are prone to structural valve degeneration (SVD). The processes involved in SVD are likely multifactorial. We hypothesized that inflammation and macrophage activation could be involved in SVD.MethodsIn 203 patients with an aortic valve biopr...
Source: Clinica Chimica Acta - July 11, 2018 Category: Laboratory Medicine Source Type: research

Triglyceride to high density lipoprotein cholesterol ratio may serve as a useful predictor of major adverse coronary event in female revascularized ST-elevation myocardial infarction
We examined the association between TG/HDL-C ratio and clinical outcome of revascularized STEMI patients in the Chinese population.Methods464 STEMI patients who underwent successful revascularization were enrolled to determine the relationship between TG/HDL-C ratio and major adverse coronary events(MACEs) with a 30-month follow-up. The Kaplan-Meier analysis and Cox regression proportional hazard model were applied to assess the prognostic value of TG/HDL-C ratio.ResultsTG/HDL-C ratio was found to be significantly associated with age (p = 0.017), history of diabetes(p = 0.017), heart rate(p = 0.011), TG(p 
Source: Clinica Chimica Acta - July 11, 2018 Category: Laboratory Medicine Source Type: research

Point-of-care identification of organophosphates in gastric juice by ambient mass spectrometry in emergency settings
ConclusionsThe results suggested that TD-ESI/MS is promising in promptly providing toxicological information to assist succeeding medical management in an emergency room. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 11, 2018 Category: Laboratory Medicine Source Type: research

The development of autoverification rules applied to urinalysis performed on the AutionMAX-SediMAX platform
ConclusionsReview rules applied to the algorithm for routine practice enhance workflow efficiency and optimize sample screening. Revision is not necessary for samples not flagged by the rules. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 11, 2018 Category: Laboratory Medicine Source Type: research

LncRNA HOXA-AS2 and its molecular mechanisms in human cancer
Publication date: Available online 4 July 2018Source: Clinica Chimica ActaAuthor(s): Jicai Wang, Zhilei Su, Shounan Lu, Wen Fu, Zhifa Liu, Xingming Jiang, Sheng TaiAbstractLong non-coding RNAs (lncRNAs), are a novel class of noncoding RNAs, and are commonly defined as RNA molecules greater more than 200 nucleotides in length. Emerging research has indicated that lncRNA played a vital role in the pathophysiology of human tumorigenesis and progression by serving as tumor oncogenes or suppressors either oncogenes or tumor suppressor genes. LncRNA has been shown to get involved in participate various biological processes, such...
Source: Clinica Chimica Acta - July 11, 2018 Category: Laboratory Medicine Source Type: research

Validation model of a laboratory-developed method for the ISO15189 accreditation: The example of salivary cortisol determination
ConclusionsA high competence is required for laboratory professionals in order to guarantee that the validated quality specifications have a positive impact on patients' management. The ISO 15189 accreditation is an important tool that allows demonstrating the value of a medical laboratory in the clinical context. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 11, 2018 Category: Laboratory Medicine Source Type: research

Urine metabolome analysis by gas chromatography–mass spectrometry (GC–MS): Standardization and optimization of protocols for urea removal and short-term sample storage
Conclusionsour study confirms the importance of urea removal for a reliable recognition and quantitation of metabolites; urine short-term storage at −80 °C should be carefully reconsidered. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 11, 2018 Category: Laboratory Medicine Source Type: research

Liquid chromatography-tandem mass spectrometry method for estimation of a panel of lysophosphatidylcholines in dried blood spots for screening of X-linked adrenoleukodystrophy
ConclusionThe LC-MS/MS method for estimating LPCs in DBS can be used to identify X-ALD in clinically suspected patients. Further large-scale studies to determine the pre-analytical variables and to define age- and gender-specific reference ranges in various ethnic groups are warranted before introducing this method for high-risk screening in India. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - July 11, 2018 Category: Laboratory Medicine Source Type: research

Long non-coding RNA CRNDE in cancer prognosis: Review and meta-analysis
Publication date: Available online 7 July 2018Source: Clinica Chimica ActaAuthor(s): Haibiao Xie, Bo Ma, Qunjun Gao, Hengji Zhan, Yuchen Liu, Zhicong Chen, Shaobo Ye, Jianfa Li, Lin Yao, Weiren HuangAbstractBackgroundColorectal neoplasia differentially expressed (CRNDE), a 1910-nt lncRNA encoded on human chromosome 16, has been found to be involved in various cancers. Nevertheless, the clinical and diagnostic values of CRNDE in tumors still need to be explored. In this review, we aimed to elucidate the clinical role of CRNDE in cancer by searching all correlative literature, and we sequentially explored the association bet...
Source: Clinica Chimica Acta - July 11, 2018 Category: Laboratory Medicine Source Type: research

Cisterno-lumbar gradient of complement fractions in geriatric patients with suspected normal pressure hydrocephalus
Publication date: Available online 10 July 2018Source: Clinica Chimica ActaAuthor(s): Jana Seele, Michael Kirschfink, Marija Djukic, Peter Lange, Johannes Gossner, Stephanie Bunkowski, Jens Wiltfang, Roland NauAbstractBackgroundThe complement system is a functional link between the innate and adaptive immune system and present in all compartments of the body. The composition of the cerebrospinal fluid (CSF) differs between the ventricular, cisternal and lumbar space. Usually, concentrations of blood-derived CSF proteins increase from ventricular to lumbar fractions.MethodsIn 20 geriatric patients with suspected normal pres...
Source: Clinica Chimica Acta - July 11, 2018 Category: Laboratory Medicine Source Type: research