Substance P in cardiovascular diseases – A bioanalytical review
Publication date: Available online 16 May 2019Source: Clinica Chimica ActaAuthor(s): Martin Feickert, Bjoern B. BurckhardtAbstractSubstance P has recently received much attention as a mediator of adverse heart remodelling and cardiac inflammation by releasing proinflammatory cytokines and matrix metalloproteases from immune and cardiac mast cells. Based on animal models, Substance P is highly associated with the development of cardiomyopathies, subsequently leading to heart failure. After a brief overview of the pathological role of Substance P in cardiac remodelling and cardiac inflammation, this review summarizes the lim...
Source: Clinica Chimica Acta - May 17, 2019 Category: Laboratory Medicine Source Type: research

Biological variation data for lipid cardiovascular risk assessment biomarkers. A systematic review applying the biological variation data critical appraisal checklist (BIVAC)
ConclusionsApplication of BIVAC identifies BV data suitable for many important applications including setting APS. Additionally, this review identifies a need for new BIVAC compliant studies to deliver BV reference data in different subpopulations. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - May 17, 2019 Category: Laboratory Medicine Source Type: research

Serum lipoprotein-associated phospholipase A2 as a promising prognostic biomarker in association with 90-day outcome of acute intracerebral hemorrhage
Publication date: Available online 16 May 2019Source: Clinica Chimica ActaAuthor(s): Lin Bian, Lian-Gang Mao, Yi Sun, Feng Shen, Jun-Feng Chen, Zheng Liu, Wei ZhouAbstractBackgroundLipoprotein-associated phospholipase A2 (Lp-PLA2) is reflective of vascular inflammation and plays a role in the pathophysiology of cerebrovascular disease. We determine usefulness of serum Lp-PLA2 as a prognostic biomarker for intracerebral hemorrhage (ICH).MethodsIn this prospective, observational study, serum Lp-PLA2 concentrations were detected among 164 patients with acute spontaneous basal ganglia hemorrhage and 164 healthy controls. Using...
Source: Clinica Chimica Acta - May 17, 2019 Category: Laboratory Medicine Source Type: research

Metabolomics workflow for lung cancer: Discovery of biomarkers
Publication date: Available online 17 May 2019Source: Clinica Chimica ActaAuthor(s): Yuqing Tang, Zhou Li, Lissy Lazar, Zhiling Fang, Chunlan Tang, Jinshun ZhaoAbstractLung cancer is one of the most common cancers in the world. Due to the limitations of current diagnostic techniques and methods, most lung cancers are diagnosed at the advanced stage, which is not conducive to early treatment. The rise of metabolomics has provided new ideas for the early diagnosis of lung cancer. As a method for the comprehensive analysis of endogenous metabolites of the biological system, metabolomics has shown significant application poten...
Source: Clinica Chimica Acta - May 17, 2019 Category: Laboratory Medicine Source Type: research

Advances in the relationship between glycosyltransferases and multidrug resistance in cancer
Publication date: Available online 15 May 2019Source: Clinica Chimica ActaAuthor(s): Yinshuang Wu, Xixi Chen, Shidan Wang, Shujing WangAbstractDespite great progress in clinical treatment, cancer remains a serious health problem contributing to significant morbidity and mortality worldwide. Although chemotherapy is a common therapeutic measure, multidrug resistance (MDR) presents a major challenge that often leads to poor prognosis. The abnormal expression of glycosyltransferases (GTs) leading to aberrant glycosylation patterns are considered a marker of cancer. Furthermore, the biosynthesis of these glycoconjugates has be...
Source: Clinica Chimica Acta - May 17, 2019 Category: Laboratory Medicine Source Type: research

Simultaneous quantification of 48 plasma amino acids by liquid chromatography-tandem mass spectrometry to investigate urea cycle disorders
Publication date: Available online 13 May 2019Source: Clinica Chimica ActaAuthor(s): Min-Zhi Peng, Yan-Na Cai, Yong-Xian Shao, Lu Zhao, Min-Yan Jiang, Yun-Ting Lin, Xi Yin, Hui-Ying Sheng, Li LiuAbstractUrea cycle disorders (UCD) are inborn errors of ammonia detoxification in which early diagnosis and treatment are critical to prevent metabolic emergencies. Unfortunately, the diagnosis was often and pronounced delayed. To improve diagnosis, we developed herein a liquid chromatography-tandem mass spectrometry method to investigate the disturbance of amino acid profile caused by UCD. The method enabled absolute quantificatio...
Source: Clinica Chimica Acta - May 14, 2019 Category: Laboratory Medicine Source Type: research

Comparison of non-fasting LDL-C levels calculated by Friedewald formula with those directly measured in Chinese patients with coronary heart disease after a daily breakfast
Publication date: Available online 11 May 2019Source: Clinica Chimica ActaAuthor(s): Qiu-zhen Lin, Yan-qiao Chen, Li-Ling Guo, Qun-Yan Xiang, Feng Tian, Tie Wen, Ling LiuAbstractBackgroundLDL-C level can be measured by direct methods (LDL-CM) or calculated by Friedewald formula (LDL-CC). The aim of this study was to investigate the difference between LDL-CM and LDL-CC after a daily breakfast in Chinese patients with coronary heart disease (CHD).MethodsThree hundred and three inpatients, including 203 CHD patients (CHD group) and 100 non-CHD controls (CON group), were enrolled in this study. Serum levels of blood lipid para...
Source: Clinica Chimica Acta - May 13, 2019 Category: Laboratory Medicine Source Type: research

Serum albumin saturation test based on non-esterified fatty acids imbalance for clinical employment
Publication date: Available online 10 May 2019Source: Clinica Chimica ActaAuthor(s): Cassiano Felippe Gonçalves-de-Albuquerque, Marcos Roberto Colombo Barnese, Mariana Alves Soares, Mauro Velho Castro Faria, Adriana Ribeiro Silva, Hugo Caire Castro Faria Neto, Patrícia Burth, Mauricio Younes-IbrahimAbstractFatty acids are fundamental as energy and structural source to the human cells. They are not usually found free in human circulation. Alteration in fatty acids metabolism is linked to diseases such as diabetes, preeclampsia, heart disease, and some infectious diseases. Increased levels of non-esterified fat...
Source: Clinica Chimica Acta - May 11, 2019 Category: Laboratory Medicine Source Type: research

Estimating the glomerular filtration rate and tubular dysfunction in an elderly population with normoalbuminuria in China
Publication date: Available online 9 May 2019Source: Clinica Chimica ActaAuthor(s): Huabin Wang, Yingjie Lou, Yongjun Ma, Xiaoyun ShanAbstractBackgroundThe prevalence and clinical risk factors of normoalbuminuric renal impairment have not yet been investigated in elderly Chinese populations. To clarify this, we conducted survey research on an elderly Chinese community population.MethodsA total of 691 elderly community participants were included in this study. Normoalbuminuria was defined as a urinary albumin to creatinine ratio (ACR) 60 and 
Source: Clinica Chimica Acta - May 10, 2019 Category: Laboratory Medicine Source Type: research

The VLDL receptor plays a key role in the metabolism of postprandial remnant lipoproteins
Publication date: Available online 10 May 2019Source: Clinica Chimica ActaAuthor(s): Katsuyuki Nakajima, Yoshiharu Tokita, Akira Tanaka, Sadao TakahashiAbstractA new concept to account for the process of postprandial remnant lipoprotein metabolism is proposed based on the characteristics of lipoprotein particles and their receptors. The characteristics of remnant lipoprotein (RLP) were investigated using an immuno-separation method. The majority of the postprandial lipoproteins increased after fat intake was shown to be VLDL remnants, not chylomicron (CM) remnants, based on the significantly high ratio of apoB100/apoB48 in...
Source: Clinica Chimica Acta - May 10, 2019 Category: Laboratory Medicine Source Type: research

Soluble neural cell adhesion molecule and behavioural recovery in minimally conscious patients undergoing transcranial direct current stimulation
ConclusionsThis exploratory research suggests the sNCAM levels, potentially associated with tDCS outcomes, as a candidate biomarker of neurobiological after-effects in MCS patients. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - May 9, 2019 Category: Laboratory Medicine Source Type: research

PCSK9: A new participant in lipophagy in regulating atherosclerosis?
Publication date: Available online 7 May 2019Source: Clinica Chimica ActaAuthor(s): Jun Xiao, Yi-Min Deng, Xiang-Rui Liu, Jian-Ping Cao, Min Zhou, Ya-Ling Tang, Wen-Hao Xiong, Zhi-Sheng Jiang, Zhi-Han Tang, Lu-Shan LiuAbstractProprotein convertase subtilisin kexin 9 (PCSK9) regulates lipid metabolism by degrading low-density lipoprotein receptor on the surface of hepatocytes. PCSK9-mediated lipid degradation is associated with lipophagy. Lipophagy is a process by which autophagosomes selectively sequester lipid-droplet-stored lipids and are delivered to lysosomes for degradation. Lipophagy was first discovered in hepatocyt...
Source: Clinica Chimica Acta - May 9, 2019 Category: Laboratory Medicine Source Type: research

Association of IL-10 to coronary disease severity in patients with metabolic syndrome
Publication date: Available online 7 May 2019Source: Clinica Chimica ActaAuthor(s): Ana Letícia Vargas Barcelos, Eduardo Aires de Oliveira, Gabriela Viegas Haute, Bruna Pasqualotto Costa, Leonardo Pedrazza, Márcio Vinícius Fagundes Donadio, Jarbas Rodrigues de Oliveira, Luiz Carlos BodaneseAbstractBackgroundMetabolic syndrome (MetS) is a group of risk factors that increase the risk for heart disease. Little is known about the role of IL-10 in the severity of coronary artery disease (CAD) in patients with MetS. We investigated plasma levels of IL-10 and other pro-inflammatory cytokines in patients with ...
Source: Clinica Chimica Acta - May 9, 2019 Category: Laboratory Medicine Source Type: research

Comparison of liquid chromatography with tandem mass spectrometry and ion-exchange chromatography by post-column ninhydrin derivatization for amino acid monitoring
Publication date: Available online 8 May 2019Source: Clinica Chimica ActaAuthor(s): Andraz Smon, Vanja Cuk, Jernej Brecelj, Simona Murko, Urh Groselj, Mojca Zerjav Tansek, Tadej Battelino, Barbka Repic LampretAbstractObjectivesPrecise quantification of amino acids (AAs) is mandatory for successful diagnosis and monitoring of patients with metabolic diseases. We compared ion-exchange chromatography (IEC) and liquid chromatography with tandem mass spectrometry (LC-MS/MS), the two methods most commonly used in clinical laboratories for the quantification of AAs in physiological samples.Design & methods123 apparently healt...
Source: Clinica Chimica Acta - May 9, 2019 Category: Laboratory Medicine Source Type: research

Corrigendum to “Detection of BRAF V600E mutation in fine-needle aspiration fluid of papillary thyroid carcinoma by droplet digital PCR” [Clinica Chimica Acta 491 (2019) 91–96]
Publication date: Available online 6 May 2019Source: Clinica Chimica ActaAuthor(s): Xiao Xu, Xiaowei Ma, Xinju Zhang, Guojun Cao, Yigui Tang, Xuan Deng, Zhihua Kang, Min Li, Ming Guan (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - May 7, 2019 Category: Laboratory Medicine Source Type: research

Increased plasma sestrin2 concentrations in patients with chronic heat failure and predicted the occurrence of major adverse cardiac events: A 36-month follow-up cohort study
ConclusionsPlasma Sesn2 concentrations were increased in CHF patients and positively correlated with the severity of CHF. Increased Sesn2 concentrations significantly increased the occurrence of major adverse cardiac events and suggested poor outcome in CHF patients. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - May 4, 2019 Category: Laboratory Medicine Source Type: research

Quantitative proteomics of changes in succinylated proteins expression profiling in left appendages tissue from valvular heart disease patients with atrial fibrillation
Publication date: Available online 3 May 2019Source: Clinica Chimica ActaAuthor(s): Fan Bai, Tao Tu, Fen Qin, Yingxu Ma, Na Liu, Yaozhong Liu, Xiaobo Liao, Shenghua Zhou, Qiming LiuAbstractBackgroundPrevious studies have suggested that proteomic modifications are closely associated with cardiovascular diseases. The aim of this study was to identify potential mechanisms by profiling the changes in succinylated protein expression in left appendage tissues from patients with valvular heart disease and atrial fibrillation (AF).MethodsUsing dimethyl labeling for relative and absolute quantification-coupled high-performance liqu...
Source: Clinica Chimica Acta - May 4, 2019 Category: Laboratory Medicine Source Type: research

Study on urine metabolic profiling and pathogenesis of hyperlipidemia
ConclusionThe non-targeted metabolomic method based on UPLC-Q-TOF/MS technology can effectively identify potential biomarkers in the urine of HLP patients and explore the possible pathogenesis. Our research will lay a foundation for finding new targets for the treatment of HLP and provide a basis for clinical research on the treatment of HLP. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - May 4, 2019 Category: Laboratory Medicine Source Type: research

A post-hoc subgroup analysis assessing acute cardiac biomarker profiles in female cancer patients during adjuvant therapy
Publication date: Available online 2 May 2019Source: Clinica Chimica ActaAuthor(s): Katharine Mackett, Sukhbinder Dhesy-Thind, Elysia K. Donovan, Som Mukherjee, Anand Swaminath, Darryl P. Leong, Sachi Voruganti, Jonathan Sussman, James Wright, Gordon Okawara, Graham Fraser, Stephen Sagar, Louise Bordeleau, Peter M. Ellis, Hal Hirte, Peter A. Kavsak (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - May 3, 2019 Category: Laboratory Medicine Source Type: research

Liquid biopsy: Circulating exosomal long noncoding RNAs in cancer
Publication date: Available online 2 May 2019Source: Clinica Chimica ActaAuthor(s): Nan Jiang, Jinchang Pan, Shuai Fang, Chengwei Zhou, Ying Han, Jun Chen, Xiaodan Meng, Xiaofeng Jin, Zhaohui GongAbstractDespite many advances in diagnostics and multimodal treatment (surgery, radiotherapy, chemotherapy), cancer still remains one of the most important public health challenges worldwide because of the associated morbidity and mortality. Liquid biopsy has been developed to detect cancer at an early stage based on minimally invasive and serial body fluid tests with the advantage of following tumor evolution in real time. Circul...
Source: Clinica Chimica Acta - May 3, 2019 Category: Laboratory Medicine Source Type: research

Parkinson's and Lewy body dementia CSF biomarkers
Publication date: Available online 30 April 2019Source: Clinica Chimica ActaAuthor(s): Lucilla Parnetti, Silvia Paciotti, Lucia Farotti, Giovanni Bellomo, Federica Nicoletta Sepe, Paolo EusebiAbstractThe clinical diagnosis of Parkinson's disease (PD) and Dementia with Lewy bodies (DLB) is challenging due to highly variable clinical presentation and clinical and pathological overlap with other neurodegenerative diseases. Since cerebrospinal fluid (CSF) mirrors the pathological changes taking place in the brain, it represents a promising source of biomarkers.With respect to classical AD biomarkers, low CSF Aβ42 levels h...
Source: Clinica Chimica Acta - May 1, 2019 Category: Laboratory Medicine Source Type: research

Diagnosis associated with Tau higher than 1200 pg/mL: Insights from the clinical and laboratory practice
Publication date: Available online 30 April 2019Source: Clinica Chimica ActaAuthor(s): S. Lehmann, C. Paquet, C. Malaplate-Armand, E. Magnin, S. Schraen, M. Quillard-Muraine, O. Bousiges, C. Delaby, J. Dumurgier, J. Hugon, B. Sablonnière, F. Blanc, D. Wallon, A. Gabelle, J.L. Laplanche, E. Bouaziz-Amar, K. Peoc'h, ePLM GroupAbstractContextCerebrospinal fluid (CSF) biomarkers are valuable tools for the diagnosis of neurological diseases. We aimed to investigate within a retrospective multicentric study the final diagnosis associated with very high CSF Tau levels and to identify patterns of biomarkers that would diffe...
Source: Clinica Chimica Acta - May 1, 2019 Category: Laboratory Medicine Source Type: research

BCL10 in cell survival after DNA damage
This article reviews the role of BCL10 in cell survival following DNA damage. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - April 30, 2019 Category: Laboratory Medicine Source Type: research

Serum netrin-1 serves as a prognostic biomarker of aneurysmal subarachnoid hemorrhage
This study was designed to discern the utility of serum netrin-1 as a biomarker for assessing the severity and prognosis of patients with aneurysmal SAH.MethodsNetrin-1 concentrations were gauged in serum from 104 patients and 104 controls. Hemorrhagic clinical and radiological severity was assessed utilizing World Federation of Neurological Surgeons (WFNS) score, modified Fisher score, and Hunt Hess score. Glasgow Outcome Scale (GOS) score was recorded at 6 months after SAH. GOS score of 1–3 was considered as a poor outcome.ResultsPatients showed substantially lower serum netrin-1 concentrations than controls (med...
Source: Clinica Chimica Acta - April 30, 2019 Category: Laboratory Medicine Source Type: research

Measurement of calcineurin activity in peripheral blood mononuclear cells by ultra-high performance liquid chromatography-tandem mass spectrometry. Renal transplant recipients application (pharmacodynamic monitoring)
Publication date: Available online 27 April 2019Source: Clinica Chimica ActaAuthor(s): Pere Fontova, Raül Rigo-Bonnin, Anna Vidal-Alabró, Gema Cerezo, Oriol Bestard, Josep M. Cruzado, Joan Torras, Josep M. Grinyó, Núria LloberasAbstractBackgroundTherapeutic drug monitoring of calcineurin inhibitor, tacrolimus (TAC), is routinely used in post-transplantation. Currently, measurement of calcineurin activity has been proposed as a promising clinical tool to evaluate efficacy and to optimize drug dosing. The main aim of our study was to develop a method to measure phosphatase calcineurin activity (CNA)...
Source: Clinica Chimica Acta - April 28, 2019 Category: Laboratory Medicine Source Type: research

The potentiality of salivary peptide biomarkers for screening patients with periodontal diseases by mass spectrometry
ConclusionsSome peptides in saliva, GCF, and serum act as biomarkers for chronic periodontitis and gingivitis, which have certain potentiality for screening patients with periodontal diseases and distinguishing them from healthy individuals in a comparatively large population by mass spectrometry. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - April 24, 2019 Category: Laboratory Medicine Source Type: research

Applying a multiplexed primer extension method on dried blood spots increased the detection of carriers at risk of glucose-6-phosphate dehydrogenase deficiency in newborn screening program
ConclusionsOur approach could significantly increase the detection rate of male patients and female carriers with a reasonable cost and a reasonable number of clinic referrals. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - April 24, 2019 Category: Laboratory Medicine Source Type: research

Evaluation of reproducibility of the cTnT immunoassay using quality control samples
Publication date: Available online 22 April 2019Source: Clinica Chimica ActaAuthor(s): Rudina Ndreu, Veronica Musetti, Silvia Masotti, Martina Zaninotto, Concetta Prontera, Giancarlo Zucchelli, Mario Plebani, Aldo Clerico, on behalf of the Italian Society of Clinical Biochemistry (SIBioC), the Italian Section of the European Ligand Assay Society (ELAS) (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - April 24, 2019 Category: Laboratory Medicine Source Type: research

Analysis of serum Haptoglobin using glycoproteomics and lectin immunoassay in liver diseases in Hepatitis B virus infection
Publication date: Available online 20 April 2019Source: Clinica Chimica ActaAuthor(s): K. Dalal, B. Dalal, S. Bhatia, A. Shukla, A. ShankarkumarAbstractBackgroundHepatocellular carcinoma (HCC) due to Hepatitis B viral (HBV) infection is major cause in Asia-Pacific countries. Its early detection is of paramount importance using a marker having both sensitivity and specificity. The present study promises diagnostic and prognostic markers by the identification of site specific glycoforms on Haptoglobin (Hp) using LC-MS/MS and lectin ELISA in liver diseased conditions in HBV infection.MethodsThree groups of patients: chronic, ...
Source: Clinica Chimica Acta - April 21, 2019 Category: Laboratory Medicine Source Type: research

Characteristics of apparently healthy individuals with a very low C-reactive protein
ConclusionvlCRP is associated with younger age, non-smoking, and absence of hyperlipidemia and of hypertension. However, it may also be part of the individual physiology. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - April 21, 2019 Category: Laboratory Medicine Source Type: research

Small but non-negligible discrepancy between subgroup and single CKD-EPI equation to calculate the estimated glomerular filtration rate
Publication date: Available online 20 April 2019Source: Clinica Chimica ActaAuthor(s): Tae-Dong Jeong (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - April 21, 2019 Category: Laboratory Medicine Source Type: research

Serum soluble TWEAK levels in severe traumatic brain injury and its prognostic significance
ConclusionsRaised serum sTWEAK levels are closely related to increasing inflammatory response, elevated trauma severity and worse clinical outcome after sTBI. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - April 21, 2019 Category: Laboratory Medicine Source Type: research

Blood and urine biomarkers in chronic kidney disease: An update
ConclusionBiomarkers from urine samples yield more significant outcome as compare to biomarkers from blood samples. But, validation and confirmation with a different type of study designed on a larger population is needed. More comparison studies on different types of samples are needed to further illuminate which biomarker is the better tool for the diagnosis and prognosis of CKD. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - April 21, 2019 Category: Laboratory Medicine Source Type: research

A comparative study of viscoelastic hemostatic assays and conventional coagulation tests in trauma patients receiving fibrinogen concentrate
Publication date: Available online 17 April 2019Source: Clinica Chimica ActaAuthor(s): Henry T. Peng, Bartolomeu Nascimento, Homer Tien, Jeannie Callum, Sandro Rizoli, Shawn G. Rhind, Andrew BeckettAbstractBackgroundBoth thrombelastography (TEG) and rotational thromboelastometry (ROTEM) have been investigated for diagnosis of coagulopathy and guidance of resuscitation in trauma and surgery. Given similarities between the two systems, it is important to determine whether one is superior to the other and how comparable they are to conventional coagulation tests (CCTs). Therefore, we conducted a comparative study of functiona...
Source: Clinica Chimica Acta - April 18, 2019 Category: Laboratory Medicine Source Type: research

Circulating PCSK9 concentrations are increased in postmenopausal women with the metabolic syndrome
Publication date: Available online 16 April 2019Source: Clinica Chimica ActaAuthor(s): Nutjaree JeenduangAbstractBackgroundHigh PCSK9 concentrations are associated with an increased risk of cardiovascular disease (CVD). We investigated PCSK9 concentrations and their association with metabolic parameters in Thai subjects and to compare PCSK9 concentrations in pre- and postmenopausal women with and without metabolic syndrome (MetS).MethodsAnthropometric data, serum lipids, fasting blood glucose (FBG), and PCSK9 concentrations were measured in 436 Thai subjects (152 men, 143 premenopausal, and 141 postmenopausal women).Result...
Source: Clinica Chimica Acta - April 18, 2019 Category: Laboratory Medicine Source Type: research

Clinical performance of non-criteria antibodies to phospholipids in Chinese patients with antiphospholipid syndrome
ConclusionsIn summary, our findings indicate that those non-criteria aPLs may be particularly helpful for patients in whom APS is highly suspected, but conventional aPLs are repeatedly negative as well as for predicting APS patients with arterial thrombosis. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - April 18, 2019 Category: Laboratory Medicine Source Type: research

Quality control optimization part II: A method to optimize the accuracy of laboratory quality control
ConclusionQC performance can be optimized for specific contexts. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - April 18, 2019 Category: Laboratory Medicine Source Type: research

A gradual change of chromosome mosaicism from placenta to fetus leading to T18 false negative result by NIPS
ConclusionFor the reason of cell-free fetal DNA (cff DNA) in the maternal circulation originates from trophoblast cells of placenta, the level of placental mosaicism could cause false negative NIPS result in multiple aneuploidies. The present study proved that a discordant T5 positive and T18 negative NIPS result was caused by placental mosaicism. This study highlights placental mosaicism as a significant risk factor for discordant NIPS results. The result will be helpful for genetic counseling and clinical management of such pregnant woman. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - April 16, 2019 Category: Laboratory Medicine Source Type: research

The development and validation of a combined kinetic fluorometric activity assay for fibroblast activation protein alpha and prolyl oligopeptidase in plasma
ConclusionWith this new assay, on average 67% of the Z-Gly-Pro-AMC converting activity in plasma can be attributed to FAP.Graphical abstract (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - April 12, 2019 Category: Laboratory Medicine Source Type: research

Cardiac biomarkers but not measures of vascular atherosclerosis predict mortality in patients with peripheral artery disease
Publication date: Available online 11 April 2019Source: Clinica Chimica ActaAuthor(s): Robert K. Clemens, Wijtske Annema, Frederic Baumann, Stephanie Roth-Zetsche, Burkhardt Seifert, Arnold von Eckardstein, Beatrice R. Amann-VestiAbstractBackgroundPeripheral artery disease (PAD) becomes more prevalent with advancing age and is associated with elevated risk of cardiovascular events and shortened life expectancy. We investigated the prognostic performance of cardiac and vascular biomarkers in a cohort of PAD patients.MethodsA total of 95 PAD patients were enrolled (mean age 68 years, range 47 to 86 years, 73 males). Caro...
Source: Clinica Chimica Acta - April 12, 2019 Category: Laboratory Medicine Source Type: research

Estradiol reference intervals in women during the menstrual cycle, postmenopausal women and men using an LC-MS/MS method
Publication date: Available online 11 April 2019Source: Clinica Chimica ActaAuthor(s): Sara J.E. Verdonk, Hubert W. Vesper, Frans Martens, Patrick M. Sluss, Jacquelien J. Hillebrand, Annemieke C. HeijboerAbstractBackgroundFor optimal medical decision-making, harmonized reference intervals for estradiol for different ages and both sexes are needed. Our aim was to establish reference intervals using a highly accurate and traceable LC-MS/MS method and to compare these with reference intervals in literature.MethodsEstradiol was measured in serum obtained daily during the menstrual cycle of 30 healthy premenopausal women and in...
Source: Clinica Chimica Acta - April 12, 2019 Category: Laboratory Medicine Source Type: research

Flow injection ionization-tandem mass spectrometry-based estimation of a panel of lysophosphatidylcholines in dried blood spots for screening of X-linked adrenoleukodystrophy
ConclusionThe FIA-MS/MS method for estimating C26:0 and C24:0-LPCs in DBS is suitable for first-tier screening of newborns for X-ALD. Second-tier confirmatory testing is required for screen positive cases. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - April 12, 2019 Category: Laboratory Medicine Source Type: research

MicroRNA-34a suppresses human lens epithelial cell proliferation and migration via downregulation of c-Met
Publication date: Available online 11 April 2019Source: Clinica Chimica ActaAuthor(s): Dong Feng, Ning Zhu, Chenying Yu, Dinghua LouAbstractMicroRNAs (miRNAs) are endogenously expressed, non-coding, small RNAs which inhibit protein translation through binding to target mRNAs. Recent studies have demonstrated that miRNAs participate in the regulation of a variety of cell structures and functions including those for cell proliferation and migration. MicroRNA-34a (miR-34a), a potential effector of the p53 tumor suppressor gene, is extensively studied for its suppression of cell growth. In the present study, we investigated th...
Source: Clinica Chimica Acta - April 12, 2019 Category: Laboratory Medicine Source Type: research

C-peptide and insulin assays with the Mindray CL-2000i: Precision and comparability with different methods
ConclusionsMindray CL-2000i shows a low imprecision while a satisfactory for IRI between Mindray 2000i and Siemens Immulite 2000 was onbserved. Overall, results emphasize the need for standardization/harmonization efforts for both C-peptide and IRI measurement. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - April 10, 2019 Category: Laboratory Medicine Source Type: research

Exosomes: A new approach to asthma pathology
Publication date: Available online 9 April 2019Source: Clinica Chimica ActaAuthor(s): José A. Cañas, Beatriz Sastre, José M. Rodrigo-Muñoz, Victoria del PozoAbstractAsthma is a chronic inflammatory disease of the airways with a complex pathophysiology, making the development of diagnostic and therapeutic tools a challenge. Exosomes are extracellular membranous nanovesicles implicated in intercellular communication. Exosome composition and cargo are highly heterogeneous depending on their cellular origin and physiological state. They contain proteins (tetraspanins, heat-shock proteins), nucleic a...
Source: Clinica Chimica Acta - April 10, 2019 Category: Laboratory Medicine Source Type: research

Rapid liquid chromatography-tandem mass spectrometry to determine very-long-chain fatty acids in human and to establish reference intervals for the Chinese population
This study aimed to establish and validate a robust liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based method to simultaneously quantify VLCFAs and provide reference intervals among Chinese individuals, quantify VLCFAs in pregnancy, and explore potential associations between plasma and amniotic fluid. Analytes were extracted via water-bath incubation with HCl and liquid-liquid extraction for 7 min. Method linearity, limit of detection/quantitation, precision, carryover, and recovery were evaluated per Clinical Laboratory Standard Institute guidelines. VLCFAs showed high precision based on low intra-run and t...
Source: Clinica Chimica Acta - April 10, 2019 Category: Laboratory Medicine Source Type: research

Quality control optimization part I: Metrics for evaluating predictive performance of quality control
ConclusionThe effectiveness of QC can be improved by considering the context in which the QC policy will be applied. Using simple assumptions, common accuracy metrics can be used to evaluate QC policy performance. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - April 9, 2019 Category: Laboratory Medicine Source Type: research

Evaluation of 99th percentile and reference change values of the hs-cTnI method using ADVIA Centaur XTP platform: A multicenter study
Publication date: Available online 9 April 2019Source: Clinica Chimica ActaAuthor(s): Aldo Clerico, Silvia Masotti, Veronica Musetti, Andrea Ripoli, Rosalia Aloe, Martina Di Pietro, Sara Rizzardi, Ruggero Dittadi, Cinzia Carrozza, Lucia Belloni, Marco Perrone, Tommaso Fasano, Canovi Simone, Antonio de Santis, Concetta Prontera, Cristina Guiotto, Domenico Cosseddu, Marco Migliardi, Sergio BernardiniAbstractBackgroundAccording to quality specifications required by international guidelines, the evaluation of the 99th URL value is a very difficult task that is usually beyond the capacity of a single laboratory. The aims of thi...
Source: Clinica Chimica Acta - April 9, 2019 Category: Laboratory Medicine Source Type: research

Extracellular vesicles in atherosclerosis
Publication date: Available online 5 April 2019Source: Clinica Chimica ActaAuthor(s): WenYi Deng, TingTing Tang, YangFeng Hou, Qian Zeng, YuFei Wang, WenJing Fan, ShunLin QuAbstractExtracellular vesicles (EVs), which exist in human blood, are increased in some inflammation-related cardiovascular diseases. EVs are involved in inflammation, immunity, signal transduction, cell survival and apoptosis, angiogenesis, thrombosis, and autophagy, all of which are highly significant for maintaining homeostasis and disease progression. Therefore, EVs are also associated with key steps in atherosclerosis, including cellular lipid meta...
Source: Clinica Chimica Acta - April 6, 2019 Category: Laboratory Medicine Source Type: research

The absent in melanoma 2 (AIM2) inflammasome in microbial infection
Publication date: Available online 5 April 2019Source: Clinica Chimica ActaAuthor(s): Wenbo Zhu, Xuyu Zu, Shuangquan Liu, Hongbo ZhangAbstractInflammasomes play a very important role in the host defense against multiple pathogenic microbes, including bacteria and viruses. Inflammasomes are multiprotein complex platforms that mediate the processing of the two most important inflammatory cytokines, pro-IL-1β and pro-IL-18, to their active forms. The inflammasome is formed by the apoptosis-associated speck-like protein containing a CARD (ASC), procaspase-1 and a sensor protein, either a NOD-like receptor (NLR) or an abse...
Source: Clinica Chimica Acta - April 6, 2019 Category: Laboratory Medicine Source Type: research