The impact of preoperative fibrinogen-albumin ratio on mortality in patients with acute ST-segment elevation myocardial infarction undergoing primary percutaneous coronary intervention
Publication date: Available online 20 February 2019Source: Clinica Chimica ActaAuthor(s): Lei Xiao, Yu Jia, Xueli Wang, He HuangAbstractBackgroundWe investigated the prognostic value of fibrinogen-albumin ratio (FAR) in patients with acute ST-segment elevation myocardial infarction (STEMI) undergoing primary percutaneous coronary intervention (pPCI) based on inflammation and hemorheology alterations and to determine whether FAR can supplement incremental predictive information to the Global Registry of Acute Coronary Events (GRACE) score.MethodsWe retrospectively analyzed 475 STEMI patients undergoing pPCI. Kaplan-Meier cu...
Source: Clinica Chimica Acta - February 21, 2019 Category: Laboratory Medicine Source Type: research

Serum GP73 combined AST and GGT reflects moderate to severe liver inflammation in chronic hepatitis B
Publication date: Available online 20 February 2019Source: Clinica Chimica ActaAuthor(s): Mingjie Yao, Leijie Wang, Hong You, Jianwen Wang, Hao Liao, Danli Yang, Shuhong Liu, Qiang Xu, Xiangmei Chen, Jidong Jia, Jingmin Zhao, Fengmin LuAbstractBackgroundNon-invasive method to identify chronic hepatitis B (CHB) patients with at least moderate inflammatory lesion but no increased ALT is an unmet need.We evaluated Golgi protein 73 (GP73) based hepatic inflammation model (HIM) as a serum surrogate for liver necroinflammatory activity, especially in those with ALT less than traditional upper concentration.MethodsThe diagnostic ...
Source: Clinica Chimica Acta - February 21, 2019 Category: Laboratory Medicine Source Type: research

Editorial board member, Aims and Scope, Publication Info.
Publication date: April 2019Source: Clinica Chimica Acta, Volume 491Author(s): (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - February 21, 2019 Category: Laboratory Medicine Source Type: research

Composition of plasmalogens in serum lipoproteins from patients with non-alcoholic steatohepatitis and their susceptibility to oxidation
ConclusionPlasmalogens could be a potential biomarker for evaluating the early stages of oxidation in NASH. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - February 20, 2019 Category: Laboratory Medicine Source Type: research

MALDI-TOF mass spectrometry distinguishes daratumumab from M-proteins
ConclusionsMALDI-TOF MS offers greater specificity compared to immunofixation for distinguishing daratumumab from M-proteins. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - February 19, 2019 Category: Laboratory Medicine Source Type: research

Relative quantification of plasma N-glycans in type II congenital disorder of glycosylation patients by mass spectrometry
ConclusionsOur methodology offers a feasible alternative to the current methods for CDG-II diagnosis by MS, which quantify glycan structures as fractions of the total summed signal across a mass spectrum, a strategy that lowers the variability of minor components. Moreover, given its sensitivity for less concentrated yet biologically relevant structures, it might assist the uncovering of novel diagnostic glycans in other CDG-II subtypes. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - February 16, 2019 Category: Laboratory Medicine Source Type: research

Gene expression changes in cervical squamous cancers following neoadjuvant interventional chemoembolization
ConclusionsGene expression signature significantly changes during TUACE therapy of cervical cancer. Theses alterations provide useful information for the development of novel treatment strategies for cervical cancers on the molecular level. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - February 15, 2019 Category: Laboratory Medicine Source Type: research

Association of vitamin D deficiency with insulin resistance in middle-aged type 2 diabetics
Publication date: Available online 14 February 2019Source: Clinica Chimica ActaAuthor(s): Neetu Mishra, Gauri Damle, Yogita Dhas, Joyita BanerjeeAbstractBackgroundVitamin D deficiency contributes to the pathophysiology of insulin resistance (IR) and type 2 diabetes mellitus (T2DM). We investigated the association of 25-hydroxyvitamin D [25(OH)D] with IR and β-cell function in middle-aged participants.MethodsWe enrolled 90 controls and 90 T2DM patients of both genders aged 30–50 y. Serum 25(OH)D, fasting plasma insulin (FPI), fasting plasma glucose (FPG), HbA1c, and lipid profile were measured by standard methods...
Source: Clinica Chimica Acta - February 15, 2019 Category: Laboratory Medicine Source Type: research

Long interspersed nuclear element-1 mobilization as a target in cancer diagnostics, prognostics and therapeutics
The objective of this review is to provide a critical examination of LINE-1 elements as companion cancer diagnostic/prognostic biomarkers and anti-cancer drug targets. In our view, there's great potential for LINE-1 serving as both forefronts, but there is a need for more mechanistic studies in the clinic as well as on the bench research to validate LINE-1 activation elements as cancer biomarkers or therapeutic targets; in different cancer types and/or stages of the disease. In this context, development of minimally invasive, reliable and sensitive diagnostic tools for LINE-1 activation elements for clinical use, is of pri...
Source: Clinica Chimica Acta - February 15, 2019 Category: Laboratory Medicine Source Type: research

Endometriosis-associated ovarian cancer: What have we learned so far?
Publication date: Available online 15 February 2019Source: Clinica Chimica ActaAuthor(s): M. Herreros-Villanueva, Chih-Chieh Chen, Eing-Mei Tsai, Tze-Kiong ErAbstractEndometriosis is defined as the presence of ectopic endometrial tissue outside of the uterine cavity, most commonly in the ovaries and peritoneum. It is a complex disease that is influenced by multiple factors. It is also a common gynecological disorder and affects approximately 10–15% of all women of reproductive age. Recent molecular and pathological studies indicate that endometriosis may serve as a precursor of ovarian cancer (endometriosis-associate...
Source: Clinica Chimica Acta - February 15, 2019 Category: Laboratory Medicine Source Type: research

Validity of a rapid and simple fluorometric tripeptidyl peptidase 1 (TPP1) assay using dried blood specimens to diagnose CLN2 disease
ConclusionsWe consider our TPP1 test on DBS to be a reliable, convenient and inexpensive tool for a first diagnostic step in suspected CLN2 disease. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - February 14, 2019 Category: Laboratory Medicine Source Type: research

Determination of serum neutrophil gelatinase-associated lipocalin as a prognostic biomarker of acute spontaneous intracerebral hemorrhage
ConclusionsEnhanced NGAL level is revealed after acute spontaneous ICH, in association with inflammatory degree and hemorrhagic severity, and intimately correlated with a worse prognosis. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - February 14, 2019 Category: Laboratory Medicine Source Type: research

Hematology reference intervals for transgender adults on stable hormone therapy
ConclusionThe hematology parameters for transgender men and women receiving stable hormone therapy should be evaluated against the cisgender male and cisgender female reference ranges, respectively and does not require concurrent sex hormone analysis. Care providers can utilize this observation to aid in interpretation of hematology laboratory values for transgender people. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - February 14, 2019 Category: Laboratory Medicine Source Type: research

Biomarkers for the prediction of early pulmonary embolism related mortality in spontaneous and provoked thrombotic disease
Publication date: Available online 12 February 2019Source: Clinica Chimica ActaAuthor(s): Ljiljana Jovanovic, Vesna Subota, Milena Stavric, Bojana Subotic, Boris Dzudovic, Natasa Novicic, Jovan Matijasevic, Milica Miric, Sonja Salinger, Natasa Markovic-Nikolic, Maja Nikolic, Vladimir Miloradovic, Ljiljana Kos, Tamara Kovacevic-Preradovic, Jelena Marinkovic, Nikola Kocev, Slobodan ObradovicAbstractFactors associated with provoked PE may influence a biomarker's predictive value for the primary outcome. The aim of this study was to investigate the value of BNP, cTnI, CRP and D-Dimer measurements taken soon after hospital admi...
Source: Clinica Chimica Acta - February 13, 2019 Category: Laboratory Medicine Source Type: research

Predictive value of preoperative lymphocyte-to-monocyte ratio for patients with upper tract urothelial carcinoma
Publication date: Available online 11 February 2019Source: Clinica Chimica ActaAuthor(s): Tao Li, Hang Xu, Lu Yang, Ping Tan, Qiang WeiAbstractBackgroundTo determine the predictive value of preoperative lymphocyte-to-monocyte ratio (LMR) for pathological and survival outcomes in upper tract urothelial carcinoma (UTUC) after radical nephroureterectomy (RNU).MethodsThis retrospective study included 704 UTUC patients between 2008 and 2017. We used a cutoff LMR of 3.6 to evaluate its relationship with oncological outcomes after RNU, using the Kaplan–Meier method and logistic regression models.ResultsDuring median follow-...
Source: Clinica Chimica Acta - February 12, 2019 Category: Laboratory Medicine Source Type: research

Practical application of the sigma-metric run size nomogram for multistage bracketed statistical quality control analysis of eight enzymes
ConclusionMultistage bracketed SQC is determined by sigma performance. Risk monitoring is significant during assaying to reduce patient risks and improve quality. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - February 8, 2019 Category: Laboratory Medicine Source Type: research

Comparison between amyloid-PET and CSF amyloid-β biomarkers in a clinical cohort with memory deficits
This study aims to correlate CSF and amyloid imaging biomarkers comparing them to cognitive measurements in order to determine whether these methods provide identical or complementary information.The study comprises 33 consecutive patients with suspected cognitive decline that underwent lumbar puncture for CSF biomarker analysis and Amyloid-PET/CT within the diagnostic evaluation of memory impairment. Amyloid PET/CTs were evaluated visually and quantitatively. CSF and imaging data were retrospectively evaluated and results were compared to cognition tests, age, gender, and ApoE status.Global cortex SUVr levels correlated h...
Source: Clinica Chimica Acta - February 6, 2019 Category: Laboratory Medicine Source Type: research

Development and validation of a LC-MS/MS assay for quantification of serum estradiol using calibrators with values assigned by the CDC reference measurement procedure
Publication date: Available online 4 February 2019Source: Clinica Chimica ActaAuthor(s): Justin Dowis, Witold Woroniecki, Deborah FrenchAbstractBackgroundEstradiol was historically measured by gas chromatography–mass spectrometry before development of immunoassays. Although immunoassays were fast requiring low sample volume, they had specificity issues. More recently, liquid chromatography-tandem mass spectrometry became the methodology of choice for estradiol quantitation in serum. However, one thing all methods have in common is lack of standardization.MethodsA LC-MS/MS assay was developed and clinically validated ...
Source: Clinica Chimica Acta - February 5, 2019 Category: Laboratory Medicine Source Type: research

A diethylpyrocarbonate-based derivatization method for the LC-MS/MS measurement of plasma arginine and its chemically related metabolites and analogs
Publication date: Available online 5 February 2019Source: Clinica Chimica ActaAuthor(s): Salvatore Sotgia, Angelo Zinellu, Panagiotis Paliogiannis, Gerard A. Pinna, Arduino A. Mangoni, Luciano Milanesi, Ciriaco CarruAbstractBackgroundChanges in NO metabolism correlate with cardiovascular risk factors and are associated with endothelial dysfunction. NO availability is regulated by nitric oxide synthase (NOS) and arginine and some chemically related metabolites and analogs have the capacity to alter NOS activity. Hence the need for analytical methods for the simultaneous assessment of these analytes.MethodsAnalytes (L-argini...
Source: Clinica Chimica Acta - February 5, 2019 Category: Laboratory Medicine Source Type: research

Deamidated gliadin peptide in pediatric patients with moderately increased tissue transglutaminase; does it help?
Publication date: Available online 3 February 2019Source: Clinica Chimica ActaAuthor(s): Jane A. Dickerson, Dale Lee, M. Cristina PachecoAbstractBackgroundDeamidated gliadin peptide (DGP) is a relatively new serologic assay used in diagnosis and monitoring of celiac disease. DGP IgG is recommended by some in pediatric patients 19 and
Source: Clinica Chimica Acta - February 4, 2019 Category: Laboratory Medicine Source Type: research

Using circular RNA SMARCA5 as a potential novel biomarker for hepatocellular carcinoma
ConclusionOur study reveals that circSMARCA5 may promote apoptosis, inhibit proliferation, invasion and metastasis of HCC cells. CircSMARCA5 may serve as a potential prediction and monitor biomarker for HCC, especially in HCC patients with AFP blow the cutoff value. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - February 2, 2019 Category: Laboratory Medicine Source Type: research

Advanced nanotechnology: An arsenal to enhance immunotherapy in fighting cancer
Publication date: Available online 31 January 2019Source: Clinica Chimica ActaAuthor(s): Chun-Ting Cheng, Gabriel Castro, Chun-Hsin Liu, Pauline LauAbstractCancer remains a major disease process with considerable healthcare and socioeconomic impact worldwide. Unfortunately, standard treatments using chemotherapy often do not effectively control cancer progression or prevent relapse. Over the past decades, the development of targeted therapies has substantially improved outcomes. Recently, immunotherapy has emerged as a new alternative for more effective cancer treatment and may even bring hope of a cure. Cancer immunothera...
Source: Clinica Chimica Acta - February 1, 2019 Category: Laboratory Medicine Source Type: research

Assessment of access hsTnI 99th percentiles upper reference limits following IFCC recommendations
ConclusionThe sex-specific 99th percentile URLs established for Access hsTnI assay were significantly lower than those declared by the manufacturer caused by the different choice of population selection, age groups and sample types: for those reasons, we maintain the 99th URLs provided by manufacturer. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - January 31, 2019 Category: Laboratory Medicine Source Type: research

A virtuous diagnostic and therapeutic roadmap triggered by a motivated and skilful urinary sediment examination
Publication date: Available online 30 January 2019Source: Clinica Chimica ActaAuthor(s): G. Garigali, G. Marra, V. Rizzo, F. De Liso, A. Berrettini, M. Daudon, G.B. FogazziAbstractIn this paper we describe how an accurate urinary sediment examination, which revealed the presence of a severe 2,8-dihydroxyadenine crystalluria, opened the way to an articulate and successful diagnostic and therapeutic roadmap for a rare and potentially severe renal disease. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - January 30, 2019 Category: Laboratory Medicine Source Type: research

Performance evaluation of a new and improved cuvette-based automated urinalysis analyzer with phase contrast microscopy
ConclusionsThe sediMAX conTRUST PRO provides very good performance in terms of RBC and WBC recognition and enumeration, and quite good performance for all other particles. Hyaline cast and pathological cast identification is fine and comparable to other automated systems, but could use further improvement. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - January 29, 2019 Category: Laboratory Medicine Source Type: research

Tissue factor pathway inhibitor in atherosclerosis
This article aims to explain the mechanism in inhibiting the development of atherosclerosis and clinical effects of TFPI, and provide new ideas for the clinical researches and mechanism studies of atherothrombosis. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - January 27, 2019 Category: Laboratory Medicine Source Type: research

Chitotriosidase on treatment-naïve patients with Gaucher disease: A genotype vs phenotype study
Publication date: Available online 26 January 2019Source: Clinica Chimica ActaAuthor(s): Fernanda Sperb-Ludwig, Bianca Lúcia Heineck, Kristiane Michelin-Tirelli, Taciane Alegra, Ida Vanessa Doederlein SchwartzAbstractBackgroundChitotriosidase (ChT) is used as a biomarker for the follow-up of patients with Gaucher disease (GD), once his activity is extremely elevated and declines during ERT. However, some variants in the CHIT1 gene affect ChT activity.MethodsTo assess association between ChT genotype, and clinical/biochemical features of GD were performed CHIT1 genotyping for: c.1049_1072dup24, p.Gly102Ser, p.Gly354A...
Source: Clinica Chimica Acta - January 27, 2019 Category: Laboratory Medicine Source Type: research

The extract, the molecular allergen or both for the in vitro diagnosis of peach and peanut sensitization?
ConclusionOur data strongly suggests to include the measurement of sIgE to Ara h9 into the diagnostic algorithm of peanut sensitization. 4.5% of the Sicilian population suspected of Peach sensitization were positive to Peach whole extract and negative to all the molecules available. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - January 26, 2019 Category: Laboratory Medicine Source Type: research

Free radicals in Alzheimer's disease: Lipid peroxidation biomarkers
Publication date: Available online 24 January 2019Source: Clinica Chimica ActaAuthor(s): Carmen Peña-Bautista, Miguel Baquero, Máximo Vento, Consuelo Cháfer-PericásAbstractAlzheimer's disease (AD), the most common form of dementia, has tremendous social and economic impact worldwide. Research has shown an association between pathologic development of AD and increased free-radical production. As such, the role of peroxidation in brain lipid damage and AD progression has received special attention. Previous studies on lipid peroxidation have improved our understanding of the unique pathophysiologi...
Source: Clinica Chimica Acta - January 25, 2019 Category: Laboratory Medicine Source Type: research

Oxysterols as a biomarker in diseases
Publication date: Available online 24 January 2019Source: Clinica Chimica ActaAuthor(s): Adam Zmysłowski, Arkadiusz SzterkAbstractCholesterol is one of the most important chemical substances as a structural element in human cells, and it is very susceptible to oxidation reactions that form oxysterol. Oxysterols exhibit almost the exact structure as cholesterol and a cholesterol precursor (7-dehydrocholesterol) with an additional hydroxyl, epoxy or ketone moiety. The oxidation reaction is performed via an enzymatic or non-enzymatic mechanism. The wide array of enzymatic oxysterols encountered in the human body varies in or...
Source: Clinica Chimica Acta - January 25, 2019 Category: Laboratory Medicine Source Type: research

Advances in antifungal drug measurement by liquid chromatography-mass spectrometry
Publication date: Available online 25 January 2019Source: Clinica Chimica ActaAuthor(s): Yu Zi Zheng, Sihe WangAbstractFungal infections, especially invasive types, have become a serious healthcare problem as the immunocompromised population increases. There are five main classes of antifungal drugs: polyenes, flucytosine, allylamines, azoles, and echinocandins. Therapeutic drug monitoring (TDM) is justified for flucytosine and triazoles due to their large inter- and intra-individual pharmacokinetic variability and their high tendency for drug-drug interactions. Available methods for measuring these drugs include bioassay,...
Source: Clinica Chimica Acta - January 25, 2019 Category: Laboratory Medicine Source Type: research

Evaluation of a commercial immunoassay for autoantibodies in Chinese Han systemic sclerosis population
Publication date: Available online 24 January 2019Source: Clinica Chimica ActaAuthor(s): Chenxi Liu, Yong Hou, Yajun Yang, Dong Xu, Liubing Li, Jing Li, Xiaoting Wen, Xiaofeng Zeng, Fengchun Zhang, Yongzhe LiAbstractObjectiveThe autoantibody profile, which varies between races, has rarely been reported in Chinese Han systemic sclerosis (SSc) patients. The present study aims to investigate the autoantibody profile of Chinese Han SSc patients using a commercial line immunoassay.MethodsThe prevalence of autoantibodies to Ro-52, PDGFR, Ku, PM75, PM100, Th/To, NOR90, Fib, RP155, RP11, CENP-B, CENP-A and Scl-70 were analyzed in ...
Source: Clinica Chimica Acta - January 24, 2019 Category: Laboratory Medicine Source Type: research

Editorial board member, Aims and Scope, Publication Info.
Publication date: March 2019Source: Clinica Chimica Acta, Volume 490Author(s): (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - January 24, 2019 Category: Laboratory Medicine Source Type: research

Has the time arrived for cerebrospinal fluid biomarkers in psychiatric disorders?
Publication date: Available online 22 January 2019Source: Clinica Chimica ActaAuthor(s): Alberto Lleó, Lucilla Parnetti, Olivia Belbin, Jens WiltfangAbstractPsychiatric disorders are currently classified, in the majority of cases, by clinical syndromes. However, advances over the last decade in imaging and biochemical biomarkers in several Central Nervous System (CNS) disorders anticipate the incorporation of some of these markers in the diagnostic work-up of psychiatric conditions. In particular, CSF biomarkers offer the possibility of detecting a wide range of pathophysiological processes in the CNS. Newer CSF mar...
Source: Clinica Chimica Acta - January 23, 2019 Category: Laboratory Medicine Source Type: research

Urine markers of renal tubular injury in idiopathic membranous nephropathy: A cross sectional study
Publication date: Available online 23 January 2019Source: Clinica Chimica ActaAuthor(s): An Changjuan, Akankwasa Gilbert, Liu Jianhua, Wang Dandan, Cheng Guixue, Zhang Jin, Xiaosong QinAbstractIntroductionIdiopathic membranous nephropathy (IMN) is a primary glomerular disease and a major cause of adult nephrotic syndrome. Presently, little is known about the capabilities of the urine markers to reflect the severity of IMN. We aimed at establishing whether urinary N-acetyl-β-glucosaminidase (NAG), Retinol binding protein (RBP), Kidney injury molecule-1 (KIM-1) and Neutrophil gelatianse-associated lipocalin (NGAL) are r...
Source: Clinica Chimica Acta - January 23, 2019 Category: Laboratory Medicine Source Type: research

Detection of BRAF V600E mutation in fine-needle aspiration fluid of papillary thyroid carcinoma by droplet digital PCR
ConclusionsOur results demonstrated that ddPCR could be used in detecting BRAF V600E mutation from FNA fluid samples with higher sensitivity and accuracy than ARMS. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - January 22, 2019 Category: Laboratory Medicine Source Type: research

Clinical application of immunomagnetic reduction for quantitative analysis of beta-subunit of human chorionic gonadotropin in blastocyst culture media to differentiate embryo quality
ConclusionsAn IMR assay can quantitatively measure β-hCG in blastocyst culture media, and may be a potential clinical tool to assist in the assessment of good blastocyst quality before embryo transfer. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - January 17, 2019 Category: Laboratory Medicine Source Type: research

Plasminogen activator inhibitor 1 in acute coronary syndromes
Publication date: Available online 17 January 2019Source: Clinica Chimica ActaAuthor(s): Pavlov Marin, Ćelap IvanaAbstractPlasminogen activator inhibitor 1 (PAI-1) is the main regulator of endogenous fibrinolysis, overriding the impact of other constituents of fibrinolysis. In plasma, it can be found in three forms: active, latent and inactive. There are numerous commercially available tests, analysing the activity of PAI-1 or the antigen level, with variable correlations between the two. PAI-1 has been extensively studied regarding incidence and outcomes of acute coronary syndromes, and showed positive association with b...
Source: Clinica Chimica Acta - January 17, 2019 Category: Laboratory Medicine Source Type: research

Molecular genetic testing and diagnosis strategies for dystrophinopathies in the era of next generation sequencing
Publication date: Available online 17 January 2019Source: Clinica Chimica ActaAuthor(s): Kuo Zhang, Xin Yang, Guigao Lin, Yanxi Han, Jinming LiAbstractDuchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) are X-linked recessive, inherited neuromuscular disorders, caused by pathogenic variants in the dystrophin gene that encodes the dystrophin protein. A number of mutations have been identified in the past years, producing dystrophin diversity and resulting in mild to severe phenotypes in patients. Mutations in the dystrophin gene can be characterized by laboratory testing to confirm a clinical diagnosis of ...
Source: Clinica Chimica Acta - January 17, 2019 Category: Laboratory Medicine Source Type: research

Ki-67 protein as a tumour proliferation marker
Publication date: Available online 14 January 2019Source: Clinica Chimica ActaAuthor(s): Sunil Sankunny Menon, Chandrasekharan Guruvayoorappan, Kunnathur Murugesan Sakthivel, Rajan Radha RasmiAbstractNewer treatment strategy based on proliferative nuclear marker Ki-67 targeted therapy holds promise for prioritized/personalized treatment options with regard to improved survival and outcome in patients with renal cancer. Over the past decade, the importance of Ki-67 in prognosis of breast cancer has been widely studied, however very few studies and literatures are available in the context of renal cancer which has an increas...
Source: Clinica Chimica Acta - January 15, 2019 Category: Laboratory Medicine Source Type: research

Systematic overestimation of human serum albumin by capillary zone electrophoresis method due to monoclonal immunoglobulin interferences
ConclusionMonoclonal immunoglobulins directly interfere with serum albumin measurement by the capillary zone electrophoresis method leading to a systematic overestimation of serum albumin concentrations proportional to the serum monoclonal immunoglobulin level. (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - January 12, 2019 Category: Laboratory Medicine Source Type: research

Novel SASS6 compound heterozygous mutations in a Chinese family with primary autosomal recessive microcephaly
Publication date: Available online 10 January 2019Source: Clinica Chimica ActaAuthor(s): Yanghui Zhang, Haoxian Li, Jialun Pang, Ying Peng, Li Shu, Hua WangAbstractPrimary autosomal recessive microcephaly (MCPH) is a rare hereditary disease characterized by congenitally small with brain circumference of the head below 3 standard deviations (SD). By far, 18 MCPH genes have been reported to be associated with the disease. SASS6 gene functioned in assembly of centrioles that the majority of MCPH genes present at the centrosome. There was only research reporting a homozygous missense mutation in SASS6 gene detected in a consan...
Source: Clinica Chimica Acta - January 11, 2019 Category: Laboratory Medicine Source Type: research

Genetic analysis of 63 Chinese patients with mucopolysaccharidosis type II: Functional characterization of seven novel IDS variants
This study described the molecular characteristics of 63 Chinese children with MPS II and investigated functional characterization of seven novel IDS variants. We analyzed mutations in the IDS gene of 63 children with MPS II. Seven novel mutations were further characterized by transient expression studies. 49 different mutations were identified in the IDS gene including 33 previously reported and 16 novel mutations. The mutation p.R443X and c.1122C > T(p.G374G) may be link to attenuated type. The novel missense mutations were predicted damaging in silico. The bioinformatic structural analysis of the novel missense m...
Source: Clinica Chimica Acta - January 11, 2019 Category: Laboratory Medicine Source Type: research

microRNAs in chronic kidney disease
Publication date: Available online 11 January 2019Source: Clinica Chimica ActaAuthor(s): Hui Zhao, Shi-Xing Ma, You-Quan Shang, Huan-Qiao Zhang, Wei SuAbstractChronic kidney disease (CKD) results in high morbidity and mortality worldwide causing a huge socioeconomic burden. MicroRNA (miRNA) exert critical regulatory functions by targeting downstream genes and have been associated with many pathophysiologic processes including CKD. In fact, many studies have shown that the expression of various miRNAs was significantly changed in CKD. Current investigations have focused on revealing the relationship between miRNAs and CKD s...
Source: Clinica Chimica Acta - January 11, 2019 Category: Laboratory Medicine Source Type: research

A prospective study on serum secreted protein acidic and rich in cysteine-like 1 as a prognostic marker for severe traumatic brain injury
Publication date: Available online 9 January 2019Source: Clinica Chimica ActaAuthor(s): Hang-Bin Huang, Song-Bin Yang, Liang-Jun Shen, Qing-Wei Lv, Mi Guo, Jing Zhou, Zhao Li, Chun-Song Yang, Li-Yun Wang, Han ZhangAbstractBackgroundSecreted protein acidic and rich in cysteine-like 1 (SPARCL1) regulates synaptic stability with upregulation throughout axonal regeneration. Our study aims to determine the correlation of serum SPARCL1 concentrations with the severity and in-hospital mortality of severe traumatic brain injury (sTBI).MethodsA total of 102 consecutively recruited patients admitted for sTBI and 102 randomly selecte...
Source: Clinica Chimica Acta - January 10, 2019 Category: Laboratory Medicine Source Type: research

LOX-1 and atherosclerotic-related diseases
Publication date: Available online 9 January 2019Source: Clinica Chimica ActaAuthor(s): Pingfei Jin, Shuyan CongAbstractLectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), a scavenger receptor of oxidized low-density lipoprotein (ox-LDL) found in various cells, plays a crucial role in the formation and progression of atherosclerotic plaques. Animal studies have suggested that LOX-1 mediates the balance between internalization and degeneration of endothelial cells, thereby contributing to various steps in the atherosclerotic process, from initiation to plaque rupture. Under pathological conditions, the extracel...
Source: Clinica Chimica Acta - January 10, 2019 Category: Laboratory Medicine Source Type: research

Editorial board member, Aims and Scope, Publication Info.
Publication date: February 2019Source: Clinica Chimica Acta, Volume 489Author(s): (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - January 10, 2019 Category: Laboratory Medicine Source Type: research

Small Leucine Rich Proteoglycans (decorin, biglycan and lumican) in cancer
Publication date: Available online 7 January 2019Source: Clinica Chimica ActaAuthor(s): Sandeep Appunni, Vivek Anand, Madhuram Khandelwal, Nidhi Gupta, Muni Rubens, Alpana SharmaAbstractThe extracellular matrix (ECM) prevents invasion of tumour cells and possesses an intrinsic mechanism to down-regulate signalling processes that promote cancer proliferation. Small Leucine Rich Proteoglycans (SLRPs) are ubiquitous ECM components involved in matrix structural organization and as such can potentially regulate cancer cell multiplication, angiogenesis and migration. Decorin, a class I SLRP that modulates collagen fibrillogenesi...
Source: Clinica Chimica Acta - January 7, 2019 Category: Laboratory Medicine Source Type: research

Changes in non-fasting concentrations of blood lipids after a daily Chinese breakfast in overweight subjects without fasting hypertriglyceridemia
Publication date: Available online 4 January 2019Source: Clinica Chimica ActaAuthor(s): Feng Tian, Qun-yan Xiang, Ming-yu Zhang, Yan-qiao Chen, Qiu-zhen Lin, Tie Wen, Ling LiuAbstractBackgroundOverweight is always accompanied by hypertriglyceridemia (HTG), but the change in non-fasting triglyceride (TG) concentration in overweight subjects without postprandial hypertriglyceridemia was unknown.MethodsConcentrations of serum lipids were measured at 2 and 4 h in matched overweight (OW group, n = 54) and control subjects (CON group, n = 55) after a daily meal. Concentrations of remnant cholesterol and non-HDL cholest...
Source: Clinica Chimica Acta - January 5, 2019 Category: Laboratory Medicine Source Type: research

Validation of a liquid chromatography-tandem mass spectrometry method for analyzing cannabinoids in oral fluid
Publication date: Available online 4 January 2019Source: Clinica Chimica ActaAuthor(s): Philip M. Sobolesky, Breland E. Smith, Jacqueline A. Hubbard, Judy Stone, Robert L. FitzgeraldAbstractA liquid chromatography tandem mass spectrometry method was developed for quantifying ten cannabinoids in oral fluid (OF). This method utilizes OF collected by the Quantisal™ device and concurrently quantifies cannabinol (CBN), cannabidiol (CBD), Δ9-tetrahydrocannabinol (THC), 11-hydroxy-Δ9-THC (11-OH-THC), 11-nor-9-carboxy-Δ9-THC (THC-COOH), 11-nor-9-carboxy-Δ9-THC glucuronide (THC-COOH-gluc), Δ9-THC...
Source: Clinica Chimica Acta - January 5, 2019 Category: Laboratory Medicine Source Type: research