Phoenixin 20 promotes neuronal mitochondrial biogenesis via CREB –PGC-1α pathway
This study showed that the presence of Phoenixin 20 promoted neuronal mitochondrial biogenesis in vitro. In cultured neuronal M17 cells, Phoenixin 20 increased the expression of mitochondrial regulators PGC-1 α, NRF-1, and TFAM at both mRNA and protein levels. The treatment of Phoenixin 20 increased the ratio of mitochondrial vs nuclear DNA (mtDNA/nDNA) and the multiple mitochondrial gene expression as revealed by increasing mRNA expression of Tomm22, Timm50, Atp5d, Ndufs3, and protein expression of NDU FB8. At a cellular level, Phoenixin 20 promoted mitochondrial respiratory rate and cellular ATP production. Mechani...
Source: Journal of Molecular Histology - March 31, 2020 Category: Laboratory Medicine Source Type: research

Effects of rutin on the oxidative stress, proliferation and osteogenic differentiation of periodontal ligament stem cells in LPS-induced inflammatory environment and the underlying mechanism
In conclusion, rutin promoted the antioxidative stress ability, proliferation and osteogenic differentiation of PDLSCs through PI3K/AKT signaling pathway in LPS-induced inflammatory environment. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - March 28, 2020 Category: Laboratory Medicine Source Type: research

EGFR inhibitor AG1478 blocks the formation of 3D structures mainly through ERK signaling pathway in Matrigel-induced 3D reconstruction of eccrine sweat gland-like structures
AbstractEGFR signaling plays important roles in the development of eccrine sweat glands. We previously demonstrate that Matrigel induces eccrine sweat gland cells to reconstruct the three-dimensional (3D) structures of eccrine sweat glands, but the mechanisms are still unknown. In the study, eccrine sweat gland cells were cultured within a 3D Matrigel, and EGFR inhibitor AG1478, or MEK1/2 inhibitor U0126, were added to the medium respectively. The morphology of the 3D-reconstructed eccrine sweat gland-like structures was observed, the localization of phospho-EGFR was detected, and protein levels of EGFR, phospho-EGFR, phos...
Source: Journal of Molecular Histology - March 26, 2020 Category: Laboratory Medicine Source Type: research

HOXB13 expression is correlated with hepatic inflammatory activity of patients with hepatic fibrosis
AbstractLiver fibrosis is a common pathological process of chronic hepatic injury, preceded by the chronic inflammation. The homeobox B13 (HOXB13) gene, a member of HOX family, plays diverse biological roles in embryonic development, carcinogenesis, and many inflammatory diseases. However, the expression of HOXB13 in chronic liver diseases including hepatic fibrosis remains to be defined. In present study, 55 patients with hepatic fibrosis, 15 patients of hepatocellular carcinoma, and 17 healthy controls were enrolled in this study. Pathological specimens were collected through liver biopsy or surgical resection. The degre...
Source: Journal of Molecular Histology - March 21, 2020 Category: Laboratory Medicine Source Type: research

Gingipains promote RANKL-induced osteoclastogenesis through the enhancement of integrin β3 in RAW264.7 cells
In conclusion, our data suggest that gingipains augmented t he differentiation and function of mature osteoclasts induced by RANKL through the increase in integrin αvβ3. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - March 19, 2020 Category: Laboratory Medicine Source Type: research

Morphologic description of male reproductive accessory glands in a mouse model of mucopolysaccharidosis type I (MPS I)
AbstractMucopolysaccharidosis type I (MPS I) is a genetic disease caused by a deficiency of the lysosomal hydrolase α-l-iduronidase (IDUA). IDUA degrades two types of glycosaminoglycans (GAGs): heparan and dermatan sulfates, important components of extracellular matrix, with signaling and structural functions. The accumulation of GAGs results in progressive physiological impairments in a variety of tissues, making MPS I a complex and multisystemic disease. Due the advent of therapeutic strategies which have increased patients ’ life expectancy, our group have been investigating the effect of IDUA deficiency on ...
Source: Journal of Molecular Histology - March 11, 2020 Category: Laboratory Medicine Source Type: research

PERK-eIF2 α-ATF4 signaling contributes to osteogenic differentiation of periodontal ligament stem cells
AbstractProtein kinase-like endoplasmic reticulum kinase (PERK) is a type I transmembrane protein located in the endoplasmic reticulum (ER). The PERK-eukaryotic initiation factor 2 α (eIF2α)-activating transcription factor 4 (ATF4) pathway has been proved to be involved in osteoblast differentiation, but the involvement of the PERK-eIF2α-ATF4 signaling pathway in osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) has remained unclear. Therefore, the aim of this study was to explore the role of PERK in osteogenic differentiation of hPDLSCs and to assess whether PERK-eIF2&alp...
Source: Journal of Molecular Histology - March 2, 2020 Category: Laboratory Medicine Source Type: research

The involvement of genes related to bile secretion pathway in rat tooth germ development
AbstractTooth formation is accomplished under strict genetic control procedures. Therefore, exploring the gene network system of tooth development has a very positive practical significance for the study of tooth tissue regeneration and the prevention and treatment of tooth abnormalities. Early bell stage is the initial phase of odontoblast formation and dentin matrix deposition in the process of tooth development. Through RNA sequencing and differential gene analysis of the rat tooth germ samples at cap stage and early bell stage, we found that the bile secretion pathway was the most significant difference signal pathway ...
Source: Journal of Molecular Histology - February 24, 2020 Category: Laboratory Medicine Source Type: research

The expression and role of PIDD in retina after optic nerve crush
AbstractTo examine the expression of P53-induced protein with a death domain (PIDD) at retina in animal model of optic nerve crush (ONC) and to investigate the role of PIDD in retinal glial activation and NF- κB activation induced by optic nerve damage, ONC animal model was established in Sprague–Dawley rats. PIDD has three isoforms (Isof); Western blot was performed to examine the expression of PIDD (Isof-1, Isof-2, and Isof-3, respectively) in retina at different time points after ONC. Retinal glial activation is closely associated with retinal neuronal death and is monitored by the expression of GFAP+ glial ...
Source: Journal of Molecular Histology - February 17, 2020 Category: Laboratory Medicine Source Type: research

Lefty-1 inhibits renal epithelial –mesenchymal transition by antagonizing the TGF-β/Smad signaling pathway
In this study, we found that Lefty-1 alleviates EMT induction through antagonizing TGF-β/Smad pathway in vivo and in vitro. In unilateral ureteral obstruction (UUO) model mice, administration of adenovirus-mediated overexpression of Lefty-1 (Ad-Lefty-1) significantly reduced TGF-β1/Smad expression and alleviated t he phenotypic transition of epithelial cells to mesenchymal cells and extracellular matrix (ECM) accumulation. In high glucose-induced rat renal tubular duct epithelial cell line (NRK-52E), EMT and ECM synthesis were alleviated with Lefty-1 treatment, which significantly inhibited TGF-β1/Smad pathw...
Source: Journal of Molecular Histology - February 17, 2020 Category: Laboratory Medicine Source Type: research

Molecular histology 2020: beyond what we can see
(Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - February 5, 2020 Category: Laboratory Medicine Source Type: research

Role of p53 deficiency in socket healing after tooth extractions
In this study, we displayed that osteogenic ability of p53-knockout (p53−/−) mice was significantly increased in the tooth extraction socket compared with wild-type (WT) counterparts. Bone marrow mesenchymal stem cells (BM-MSCs) from mandibular were collected and exhibited with elevated proliferation potential and colony-forming units compared with the control, as well as stronger mineral deposits and osteogenic markers. Besides, the bone mass and bone parameter in p53−/− mice were markedly enhanced compared with the counterpart after extractions by micro-CT. Masson ’s trichrome staining and i...
Source: Journal of Molecular Histology - January 31, 2020 Category: Laboratory Medicine Source Type: research

Age-dependent role of SIRT6 in jawbone via regulating senescence and autophagy of bone marrow stromal cells
AbstractAge-related jawbone loss directly impact the function of oral cavity resulted from tooth loss, implant failure, and jaw fracture. Numerous evidences show that age-related senescence of bone marrow stromal cells (BMSCs) play a critical role in bone loss, but little attention has been paid to jawbone. Here, we delineated the critical role of sirtuin family protein 6 (SIRT6) in senescence, autophagy, and osteogenesis of BMSCs from jawbones. Radiography analysis showed less jawbone quality in elderly than young people. We also showed that SIRT6 expression decreased in bone tissue and BMSCs from the elderly by immunoche...
Source: Journal of Molecular Histology - January 30, 2020 Category: Laboratory Medicine Source Type: research

K31 as a novel marker for clear secretory cells in human eccrine sweat glands
AbstractK31 was previously considered as one of the hair keratins. During a study on differential markers between hair follicles and eccrine sweat glands, we observed that K31 was expressed in eccrine sweat gland cells in a scattered pattern, similar to the distribution of dark or clear secretory cells. To investigate the precise cell localization of K31 in human eccrine sweat glands and find new marker for eccrine sweat gland cells, human skin samples were fixed, paraffined and sectioned. The serial sections were stained for K31, dark secretory cell marker gross cystic disease fluid protein 15 (GCDFP15) and clear secretor...
Source: Journal of Molecular Histology - January 23, 2020 Category: Laboratory Medicine Source Type: research

Disruption of tight junction structure contributes to secretory dysfunction in IgG4-related sialadenitis
AbstractIgG4-related sialadenitis (IgG4-RS) is a chronic fibro-inflammatory disease characterized by swelling of salivary glands and varying degrees of xerostomia. Tight junctions (TJs) play an essential role in maintaining secretory function by regulating the paracellular flow of ions and water. However, whether TJs are altered and contribute to the hyposecretion in IgG4-RS is not fully understood. Here, a total of 399 differentially expressed proteins were identified in IgG4-RS submandibular glands (SMGs) and enriched in the regulation of actin cytoskeleton and the salivary secretion. Real-time PCR results showed that th...
Source: Journal of Molecular Histology - December 21, 2019 Category: Laboratory Medicine Source Type: research

Identification of interactive molecules between antler stem cells and dermal papilla cells using an in vitro co-culture system
AbstractDeer antlers are the only mammalian organs capable of complete renewal. Antler renewal is a stem cell-based [antler stem cells (ASCs)] process. Maintenance and activation of the ASCs require them to be located in a specialized microenvironment (niche), and to interact with the cells resident in the niche. Based on previous experiments we found that niche of the ASCs is provided by the closely associated enveloping skin, which currently was known includes dermal papilla cells (DPCs) and epidermal cells. Antler generation/regeneration are triggered by the interactions between ASCs and the niche. In the present study,...
Source: Journal of Molecular Histology - December 19, 2019 Category: Laboratory Medicine Source Type: research

Natriuretic peptide system expression in murine and human submandibular salivary glands: a study of the spatial localisation of ANB, BNP, CNP and their receptors
AbstractThe natriuretic peptide (NP) system comprises of three ligands, the Atrial Natriuretic Peptide (ANP), Brain Natriuretic peptide (BNP) and C-type Natriuretic peptide (CNP), and three natriuretic peptide receptors, NPRA, NPRB and NPRC. Here we present a comprehensive study of the natriuretic peptide system in healthy murine and human submandibular salivary glands (SMGs). We show CNP is the dominant NP in mouse and human SMG and is expressed together with NP receptors in ducts, autonomic nerves and the microvasculature of the gland, suggesting CNP autocrine signalling may take place in some of these glandular structur...
Source: Journal of Molecular Histology - November 13, 2019 Category: Laboratory Medicine Source Type: research

Investigating time dependent brain distribution of nevirapine via mass spectrometric imaging
In this study the aims were to investigate the BBB membrane penetration and brain localization patterns of Nevirapine (NVP) using Imaging Mass Spectrometry (MSI). Sprague–Dawley rats received an intraperit oneal dose of NVP (50 mg kg−1). Plasma and brain samples were harvested, and mass spectrometric methods were then applied to determine the pharmacokinetic properties and localization patterns of NVP in the brain. The pharmacokinetic parameters demonstrated a rapid bio-distribution of NVP in plasma and brain. The plasma Cmax was 6320  ng mL−1 and the brain Cmax was 1923  ng ...
Source: Journal of Molecular Histology - November 4, 2019 Category: Laboratory Medicine Source Type: research

FAM20A is essential for amelogenesis, but is dispensable for dentinogenesis
In this study, we generated aFam20a knockout (Sox2-Cre;Fam20afl/fl) mouse model by crossingFam20afl/fl mice withSox2-Cre transgenic mice, in whichFam20a was ablated in both dental epithelium and dental mesenchyme. We found that these mice developed an enamel phenotype that resembles human AI associated with FAM20A mutations, but did not have apparent dentin defects. The secretory stage ameloblasts in the mandibular incisors from theSox2-Cre;Fam20afl/fl mice were shorter and detached from the enamel matrix, and subsequently lost their polarity, became disorganized and formed numerous spherical extracellular matrices in plac...
Source: Journal of Molecular Histology - October 30, 2019 Category: Laboratory Medicine Source Type: research

A simple, web-based repository for the management, access and analysis of micrographic images
AbstractMicroscopy is advancing at a rapid pace, enabling high-speed, high-resolution analyses to be conducted in a wide range of cellular contexts. For example, the capacity to quickly capture high-resolution images from multiple optical sections over multiple channels with confocal microscopy has allowed researchers to gain deeper understanding of tissue morphology via techniques such as three-dimensional rendering, as have more recent advances such as lattice light sheet microscopy and superresolution structured illumination microscopy. With this, though, comes the challenge of storing, curating, analysing and sharing d...
Source: Journal of Molecular Histology - October 30, 2019 Category: Laboratory Medicine Source Type: research

Smad interacting protein 1 influences transforming growth factor- β 1 /Smad signaling in extracellular matrix protein production and hypertrophic scar formation
This study investigated the role of the SIP1-mediated TGF- β1/Smad signaling pathway in extracellular matrix (ECM) protein production and hypertrophic scarring. SIP1 expression was markedly lower in HS vs. normal skin (NS) tissue, and α-smooth muscle actin (α-SMA) content and collagen I/III (Col I/III) synthesis were inversely correlated with SIP1 expression. Furthermore, SIP1 inhibited Smad2/3 phosphorylation in vitro, and improved the collagen-based architecture of the scar while reducing collagen expression and overall scar formation in a rabbit ear model of HS. Based on these findings, we propose that ...
Source: Journal of Molecular Histology - October 8, 2019 Category: Laboratory Medicine Source Type: research

Localization of Mucin 1 in endometrial luminal epithelium and its expression in women with reproductive failure during implantation window
In conclusion, MUC1 is mainl y expressed in ciliated cells, not secretory cells and pinopodes, of the endometrial luminal epithelium during implantation window. The specific expression of MUC1 in the ciliated cells in receptive control women is higher than that of women with reproductive failure during implantation window. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - September 25, 2019 Category: Laboratory Medicine Source Type: research

Immunohistochemical localization and possible functions of nesfatin-1 in the testis of mice during pubertal development and sexual maturation
AbstractThe study was aimed to address the role of nesfatin-1 on the sexual maturation of testis during the pubertal transition. The immunostaining of testis suggested nesfatin-1 is expressed in Leydig cells with pubertal maturation. The pre-pubertal mice for in vivo study were randomly divided in three groups; (a) control-saline (b) treated with low (0.25  nM) dose of nesfatin-1/gbw/day and (c) treated with high (1.25 nM) dose nesfatin-1/gbw/day. Histological analysis showed that nesfatin-1 loaded mice showed facilitated maturation of testis. Western blot analysis on various protein expressions upon injection of...
Source: Journal of Molecular Histology - September 20, 2019 Category: Laboratory Medicine Source Type: research

PGE2 facilitates tail regeneration via activation of Wnt signaling in Gekko japonicus
AbstractTail regeneration is a distinguishing feature of lizards; however, the mechanisms underlying tail regeneration remain elusive. Prostaglandin E2 (PGE2) is an arachidonic acid metabolite that has been extensively investigated in the inflammatory response under both physiological and pathological conditions. PGE2 also act as a regulator of hematopoietic stem cell homeostasis by interacting with Wnt signaling molecules. The present study aims to identify the effects of PGE2 on tail regeneration and the molecular mechanisms behind it. We initially found that PGE2 levels increased during the early stages of tail regenera...
Source: Journal of Molecular Histology - September 18, 2019 Category: Laboratory Medicine Source Type: research

Periostin-modified bone marrow mesenchymal stem cells from osteoporotic rats promote alveolar bone regeneration
In conclusion, Postn-modified OVX-BMMSCs might restore the osteogenic capacity and promote alveolar bone regeneration, which may serve as a new therapeutic approach for bone regeneration in osteoporosis. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - September 14, 2019 Category: Laboratory Medicine Source Type: research

Structural organization, GABAergic and tyrosine hydroxylase expression in the striatum and globus pallidus of the South American plains vizcacha, Lagostomus maximus (Rodentia, Caviomorpha)
AbstractThe striatum is an essential component of the basal ganglia that regulatessensory processing, motor, cognition, and behavior. Depending on the species, the striatum shows a unique structure called caudate –putamen as in mice, or its separation into two regions called caudate and lenticular nuclei, the latter formed by putamen and globus pallidus areas, as in primates. These structures have two compartments, striosome and matrix. We investigated the structural organization, GABAergic and tyrosine hy droxylase (TH) expression in the striatum and globus pallidus of the South American plains vizcacha,Lagostomus m...
Source: Journal of Molecular Histology - September 12, 2019 Category: Laboratory Medicine Source Type: research

Tunneling nanotubes mediate intercellular communication between endothelial progenitor cells and osteoclast precursors
This study was designed to investigate the existence of TNTs between EPCs and osteoclast precursors and evaluate their effects on the differentiation of osteoclast precursors. For these purposes, EPCs and osteoclast precursors (RAW264.7 cells) were stained with different fluorescent dyes before direct co-culture; then, the co-cultured cells were sorted by fluorescence activated cell sorter (FACS), and the differentiation of co-cultured RAW264.7 cells was evaluated. The results showed that the differentiation potential of RAW264.7 cells was significantly inhibited after their co-culture with EPCs. Additionally, the expressi...
Source: Journal of Molecular Histology - August 28, 2019 Category: Laboratory Medicine Source Type: research

NFIC promotes the vitality and osteogenic differentiation of rat dental follicle cells
In conclusion, these results suggested that upregulation of NFIC may promote the proliferation and osteogenic/cementogenic differentiation of DFCs. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - August 20, 2019 Category: Laboratory Medicine Source Type: research

Expression of Ihh signaling pathway in condylar cartilage after bite-raising in adult rats
In conclusion, our results indicated that the Ihh signaling pathway may activate the occurrence of TMJOA by mediating the hypertrophy of chondrocytes, which may be an important regulatory mechanism and potential therapeutic target in the repair of condylar cartilage. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - July 13, 2019 Category: Laboratory Medicine Source Type: research

Cytoarchitecture of the dorsal claustrum of the cat: a quantitative Golgi study
In conclusion, we have presented a detailed analysis of the cytoarchitecture of the DC of the cat and have reported the first quantitative data on a number of morphometric parameters. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - July 13, 2019 Category: Laboratory Medicine Source Type: research

Low temperature culture enhances ameloblastic differentiation of human keratinocyte stem cells
In this study, we systematically investigated the effects of low temperature on the viability, proliferation and stemness of human keratinocytes stem cells (hKSCs) in cell culture and further examined ameloblastic differentiation of the hKSCs in human –mouse recombinant chimeric tooth germs. Our results demonstrated that low temperature indeed reduces growth rate and maintains healthy undifferentiated morphology of hKSCs without any effects on cell viability. Moreover, examination of stemness makers revealed improved stemness of hKSCs cultured at low temperature with increased expression of stemness markers K15, CD29...
Source: Journal of Molecular Histology - July 5, 2019 Category: Laboratory Medicine Source Type: research

Semaphorin 3A gets involved in the establishment of mouse tooth  eruptive pathway
This study is to elucidate the roles of Semaphorin 3A (Sema 3A) in mouse tooth eruptive pathway. C57BL/6 mice (11 –13 and 15–17 days after birth) were chosen to observe eruptive pathway of mouse lower first molar. Expressions of Sema 3A and its receptor neuropilin 1 and plexin A1 were detected. Osteoclasts were identified by TRAP staining. Co-localization of Sema 3A and osteoclast maker CD68 was detected b y double immunofluorescence staining. Picrosirius red staining was applied to observe collagen fibers during mucosal penetration phase. In vitro, Bone marrow-derived macrophages (BMMs) were prepared from...
Source: Journal of Molecular Histology - July 3, 2019 Category: Laboratory Medicine Source Type: research

Inhibitory effect of melatonin on Mst1 ameliorates myocarditis through attenuating ER stress and mitochondrial dysfunction
AbstractViral myocarditis has been found to be one of the leading causes of sudden death in young adults. However, no effective drugs have been developed to intervene the progression of myocarditis. Accordingly, the present study is carried out to explore the protective role played by melatonin in the setting of viral myocarditis with a focus on Mst1-Hippo pathway, mitochondrial dysfunction and ER stress. Cardiac function was determined via echocardiographic examination. Mitochondrial function and ER stress were detected via ELISA, western blots, and immunofluorescence. Our data demonstrated that virus injection induced ca...
Source: Journal of Molecular Histology - June 29, 2019 Category: Laboratory Medicine Source Type: research

Faster regeneration associated to high expression of Fam65b and Hdac6 in dysferlin-deficient mouse
AbstractDysferlin is a sarcolemmal muscle protein associated with the processes of membrane repair, trafficking, and fusion of intracellular vesicles and muscle regeneration.  Mutations in theDYSF gene cause clinically distinct forms of muscular dystrophies. The dysferlin-deficientSJL/J mouse model presents a reduction of 85% of the protein but shows mild weakness and discrete histopathological alterations. To study the effect of dysferlin deficiency in the muscle regenerative process, we used a model of electrical injury by electroporation to induce muscle degeneration/regeneration in theSJL/J mouse. The relative exp...
Source: Journal of Molecular Histology - June 19, 2019 Category: Laboratory Medicine Source Type: research

Osteoclastogenesis from bone marrow cells during estrogen-induced medullary bone formation in Japanese quails
AbstractOsteoclasts are differentiated from hematopoietic mononuclear cells by regulation of the receptor activator of nuclear factor kappa-B (RANK)/receptor activator of nuclear factor kappa-B ligand (RANKL)/osteoprotegerin (OPG) system. Medullary bone (MB) that forms in the bone marrow of female birds is remodeled under the control of circulating estrogen (E2) during the laying period. Although the osteoclasts of MB are differentiated from mononuclear cells, the mechanism of osteoclastogenesis is not known. We investigated whether MB osteoclastogenesis is regulated by the RANK/RANKL/OPG system using MB from male quails i...
Source: Journal of Molecular Histology - June 18, 2019 Category: Laboratory Medicine Source Type: research

MIF/CD74 axis participates in inflammatory activation of Schwann cells following sciatic nerve injury
AbstractBased on deep RNA sequencing of distal segments of lesioned sciatic nerves, a huge number of differentially expression genes (DEGs) were thus obtained and functionally analyzed. The inflammatory response was denoted as one of most significant biological processes following sciatic nerve injury. In the present study, ingenuity pathway analysis (IPA) demonstrated that macrophage migration inhibitory factor (MIF) was identified as a core regulator of inflammatory response through interaction with CD74 membrane receptor. By establishment of rat sciatic nerve transection model, we displayed that MIF was upregulated foll...
Source: Journal of Molecular Histology - June 13, 2019 Category: Laboratory Medicine Source Type: research

Akt plays indispensable roles during the first cell lineage differentiation of mouse
AbstractThe first cell lineage differentiation occurs during the development of mouse 8-cell embryo to blastocyst. Akt is a potent kinase whose role during blastocyst formation has not been elucidated. In the present study, immunofluorescence results showed that the Akt protein was specifically localized to the outer cells of the morula. Akt-specific inhibitor MK2206 significantly inhibited mouse blastocyst formation and resulted in decreased expression of the trophectoderm marker Cdx2 and led to granular distribution of ER α in the cytoplasm. Furthermore, knockdown of ERα by siRNA microinjection can also lead ...
Source: Journal of Molecular Histology - June 13, 2019 Category: Laboratory Medicine Source Type: research

In vitro assessment of PD-L1+ microvesicles in the cyst fluid of non-syndromic odontogenic keratocysts
In conclusion, the present study suggests that elevated PD-L1+ cyst fluid microvesicles might be related with the cyst development of OKCs. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - May 21, 2019 Category: Laboratory Medicine Source Type: research

Tamarixetin protects against cardiac hypertrophy via inhibiting NFAT and AKT pathway
AbstractCardiac hypertrophy is a compensatory response in reaction to mechanical load that reduces wall stress by increasing wall thickness. Chronic hypertrophic remodeling involves cardiac dysfunction that will lead to heart failure and ultimately death. Studies have been carried out on cardiac hypertrophy for years, whereas the mechanisms have not been well defined. Tamarixetin (TAM), a natural flavonoid derivative of quercetin, have been demonstrated possessing anti-oxidative and anti-inflammatory effects on multiple diseases. However, little is known about the function of TAM on the development of cardiac hypertrophy. ...
Source: Journal of Molecular Histology - May 20, 2019 Category: Laboratory Medicine Source Type: research

MicroRNA-494 targets PTEN and suppresses PI3K/AKT pathway to alleviate hypertrophic scar formation
In conclusion, we confirmed that microRNA-494 could be a key regulator to suppress hypertrophic scar formation. The suppression of microRNA-494 could eliminate its inhibition effect to PTEN and finally decrease the expression of collagen and inhibit hypertrophic scar formation. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - May 8, 2019 Category: Laboratory Medicine Source Type: research

Differential antigen expression between human eccrine sweat glands and hair follicles/pilosebaceous units
AbstractEccrine sweat glands and hair follicles are two primary skin appendages that serve different functions. Although the two appendages exhibit unique morphological patterns in adults, it is difficult to distinguish them morphologically in the early stages of development and regeneration. To research and compare the development, differentiation and regeneration between eccrine sweat glands and hair follicles/pilosebaceous units, specific antigen markers must be found first. Human skin samples were fixed, paraffin-embedded, and cut. The expression of K5, K7, K8, K14, K27, K31, K73, AE13, α-smooth muscle actin (&al...
Source: Journal of Molecular Histology - May 6, 2019 Category: Laboratory Medicine Source Type: research

Hyperinsulinemia-induced KLF5 mediates endothelial angiogenic dysfunction in diabetic endothelial cells
AbstractReduced expression of endothelial nitric oxide synthase (eNOS) is a hallmark of endothelial dysfunction in diabetes, which predisposes diabetic patients to numerous cardiovascular complications including blunted angiogenesis. The Kr üppel-like factor (KLF) five has been implicated as a central regulator of cardiovascular remodeling, but its role in endothelial cells (ECs) remains poorly understood. We show here that expression of endothelial KLF5 was significantly increased in the ECs from mouse diabetes mellitus type 2 (T2DM) model, when compared to non-diabetic or T1DM mouse. KLF5 up-regulation by insulin wa...
Source: Journal of Molecular Histology - May 2, 2019 Category: Laboratory Medicine Source Type: research

Role of mTOR complex in IGF-1 induced neural differentiation of DPSCs
This study has expanded the role of mTOR in DPSCs neural differentiation regulated by IGF-1. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - May 2, 2019 Category: Laboratory Medicine Source Type: research

Impaired autophagy mediates hyperhomocysteinemia-induced HA-VSMC phenotypic switching
In conclusion, these results demonstrated that the KLF4-dependent rapamycin signaling pathway is a novel mech anism underlying HA-VSMC phenotypic switching and is crucial for HHcy-induced HA-VSMCs with defective autophagy to accelerate early atherosclerosis. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - April 26, 2019 Category: Laboratory Medicine Source Type: research

Oligoprotective effect of metformin through the AMPK-dependent on restoration of mitochondrial hemostasis in the cuprizone-induced multiple sclerosis model
AbstractOxidative stress with mitochondrial defects has a central role in the development and deterioration of Multiple sclerosis (MS). According to new findings of the effects of metformin on mitochondrial function, has attracted a lot of attention. Furthermore, it is suggested that metformin exerts its beneficial influence through AMP-activated protein kinase (AMPK) pathway. In the current study, we investigated the possible protective effects of metformin on oxidative stress and mitochondrial function by activating the AMPK pathway in the cuprizone-induced demyelination. Mice were fed with cuprizone for 6  weeks. A...
Source: Journal of Molecular Histology - April 23, 2019 Category: Laboratory Medicine Source Type: research

Rilpivirine as a potential candidate for the treatment of HIV-associated neurocognitive disorders (HAND)
This study has shown th at RPV has an excellent blood–brain barrier penetrability. Thus, in combination with other antiretroviral drugs, better central nervous system (CNS) protection against HAND can possibly be achieved. (Source: Journal of Molecular Histology)
Source: Journal of Molecular Histology - April 22, 2019 Category: Laboratory Medicine Source Type: research

Type XI collagen –perlecan–HS interactions stabilise the pericellular matrix of annulus fibrosus cells and chondrocytes providing matrix stabilisation and homeostasis
AbstractThe aim of this study was to ascertain whether, like many cell types in cartilaginous tissues if type XI collagen was a pericellular component of annulus fibrosus (AF) cells and chondrocytes. Fine fibrillar networks were visualised which were perlecan, HS (MAb 10E4) and type XI collagen positive. Heparitinase-III pre-digestion abolished the type XI collagen and 10E4 localisation in these fibrillar assemblies demonstrating a putative HS mediated interaction which localised the type XI collagen. Type XI collagen was confirmed to be present in the Heparitinase III treated AF monolayer media samples by immunoblotting. ...
Source: Journal of Molecular Histology - April 16, 2019 Category: Laboratory Medicine Source Type: research

Effects of Nel-like molecule-1 and bone morphogenetic protein 2 combination on rat pulp repair
This study aimed to evaluate the combined effects of NELL-1 and BMP2 on rat pulp repair. The experiment used healthy non-carious maxillary first molars from 60 Wistar rats. Exposed pulps were capped with NELL-1 plus BMP2, NELL-1 alone, and BMP2 alone, and each was absorbed onto a sterile collagen sponge. In the control samples, the collagen sponge alone and Dycal were used as capping agents. After l, 2 and 4  weeks, the rats were sacrificed. The formation of reparative dentin, as well the situation of pulp repair, was detected by hematoxylin-eosin (HE) staining; moreover, the expression of dentin specific protein-dent...
Source: Journal of Molecular Histology - April 1, 2019 Category: Laboratory Medicine Source Type: research

Changes in mechanoreceptors in rabbits ’ anterior cruciate ligaments with age
In this study, we observed the changes in mechanoreceptors of healthy rabbits ’ ACL with age. We found that rabbits’ ACLs contained 5 kinds of mechanoreceptors including Ruffini corpuscles, Pacinian corpuscles, Golgitendon bodies, free nerve endings and atypical mechanoreceptors. In each ACL, free nerve endings were the most followed by Ruffini corpuscles, Pacinian corpus cles, Golgitendon bodies and atypical mechanoreceptors in the younger than one-old rabbits. Most of the mechanoreceptors were distributed in the synovium near the attachment points of ACL with the femur and tibia. The total quantity of mechano...
Source: Journal of Molecular Histology - March 29, 2019 Category: Laboratory Medicine Source Type: research

Distribution of sperm antigen 6 (SPAG6) and 16 (SPAG16) in mouse ciliated and non-ciliated tissues
AbstractThe cilia and flagella of eukaryotic cells serve many functions, exhibiting remarkable conservation of both structure and molecular composition in widely divergent eukaryotic organisms. SPAG6 and SPAG16 are the homologous in the mice toChlamydomonas reinhardtii PF16 and PF20. Both proteins are associated with the axonemal central apparatus and are essential for ciliary and flagellar motility in mammals. Recent data derived from high-throughput studies revealed expression of these genes in tissues that do not contain motile cilia. However, the distribution of SPAG6 and SPAG16 in ciliated and non-ciliated tissues is ...
Source: Journal of Molecular Histology - March 25, 2019 Category: Laboratory Medicine Source Type: research