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Specialty: Laboratory Medicine

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Total 432 results found since Jan 2013.

siRNA therapeutics for breast cancer: recent efforts in targeting metastasis, drug resistance, and immune evasion.
Abstract Small interfering RNA (siRNA) has an established and precise mode of action to achieve protein knockdown. With the ability to target any protein, it is very attractive as a potential therapeutic for a plethora of diseases driven by the (over)expression of certain proteins. Utilizing siRNA to understand and treat cancer, a disease largely driven by genetic aberration, is thus actively investigated. However, the main hurdle for the clinical translation of siRNA therapeutics is to achieve effective delivery of siRNA molecules to tumors and the site of action, the cytosol, within cancer cells. Several nanopar...
Source: Translational Research : the journal of laboratory and clinical medicine - August 18, 2019 Category: Laboratory Medicine Authors: Ngamcherdtrakul W, Yantasee W Tags: Transl Res Source Type: research

Efficient nanocarriers of siRNA therapeutics for cancer treatment.
Abstract Nanocarriers as drug delivery systems are promising and becoming popular, especially for cancer treatment. In addition to improving the pharmacokinetics of poorly soluble hydrophobic drugs by solubilizing them in a hydrophobic core, nanocarriers allow cancer-specific combination drug deliveries by inherent passive targeting phenomena and adoption of active targeting strategies. Nanoparticle-drug formulations can enhance the safety, pharmacokinetic profiles, and bioavailability of locally or systemically administered drugs, leading to improved therapeutic efficacy. Gene silencing by RNA interference (RNAi)...
Source: Translational Research : the journal of laboratory and clinical medicine - July 21, 2019 Category: Laboratory Medicine Authors: Subhan MA, Torchilin VP Tags: Transl Res Source Type: research

Aquaporin-4 Blockage by siRNA Protects Rat Articular Chondrocytes from IL-1 β-induced Apoptosis by Inhibiting p38 MAPK Signal Pathway.
Aquaporin-4 Blockage by siRNA Protects Rat Articular Chondrocytes from IL-1β-induced Apoptosis by Inhibiting p38 MAPK Signal Pathway. Ann Clin Lab Sci. 2017 Sep;47(5):563-571 Authors: Cai L, Lei C, Li R, Chen WN, Li CM Abstract Accumulating evidence reveals that articular chondrocytes undergo increased apoptosis in rheumatoid arthritis (RA) and inhibiting chondrocyte apoptosis might be a promising therapeutic strategy. We recently found that aquaporin-4 (AQP4) protein level in the cartilage of rats with adjuvant-induced arthritis was higher than normal rats. Herein, cultured rat articular chondrocyte...
Source: Annals of Clinical and Laboratory Science - September 1, 2017 Category: Laboratory Medicine Authors: Cai L, Lei C, Li R, Chen WN, Li CM Tags: Ann Clin Lab Sci Source Type: research

Peptide carriers to the rescue: overcoming the barriers to siRNA delivery for cancer treatment.
Abstract Cancer is a significant health concern worldwide and its clinical treatment presents many challenges. Consequently, much research effort has focused on the development of new anticancer drugs to combat this disease. One area of exploration, in particular, has been in the therapeutic application of RNA interference (RNAi). Although RNAi appears to be an attractive therapeutic tool for the treatment of cancer, one of the primary obstacles towards its pervasive use in the clinic has been cell/tissue type-specific cytosolic delivery of therapeutic small interfering RNA (siRNA) molecules. Consequently, varied ...
Source: Translational Research : the journal of laboratory and clinical medicine - July 28, 2019 Category: Laboratory Medicine Authors: Cummings JC, Zhang H, Jakymiw A Tags: Transl Res Source Type: research

Erratum to "siRNA therapeutics for breast cancer: recent efforts in targeting metastasis, drug resistance, and immune evasion" Translational Research 214C (2019) 105-120.
PMID: 32081297 [PubMed - in process]
Source: Translational Research : the journal of laboratory and clinical medicine - February 22, 2020 Category: Laboratory Medicine Authors: Ngamcherdtrakul W, Yantasee W Tags: Transl Res Source Type: research

Effect of Small Interference RNA on the Acetylcholine-Sensitive Potassium Channel in H9c2 Cells.
CONCLUSION: siRNA could effectively inhibit the IK(ACh) channel and is a potential therapy for atrial fibrillation. PMID: 25696012 [PubMed - as supplied by publisher]
Source: Annals of Clinical and Laboratory Science - January 1, 2015 Category: Laboratory Medicine Authors: He F, Zhao J, Li L, Guo R Tags: Ann Clin Lab Sci Source Type: research

Primary cilia-dependent signaling is involved in regulating mesenchymal stem cell proliferation and pluripotency maintenance
This study confirms that MSCs have primary cilia and provides evidence that primary cilia-dependent signaling pathways play functional roles in MSCs proliferation and stemness maintenance.
Source: Journal of Molecular Histology - May 11, 2020 Category: Laboratory Medicine Source Type: research

Silencing of TGIF1 in bone mesenchymal stem cells applied to the post-operative rotator cuff improves both functional and histologic outcomes
Abstract Stem cells have long been hypothesized to improve outcomes following rotator cuff repair. However, these cells must be signaled in order to do so. TGIF1 is a transcription factor that has been found to be down-regulated in cells involved in chondrogenesis. We therefore wished to examine whether stem cells expressing lower levels of TGIF1 could better improve outcomes following rotator cuff repair than stem cells expressing normal levels of TGIF1. Bone mesenchymal stem cells (BMSCs) were transduced with TGIF1 siRNA to suppress native TGIF1. Nontransduced BMSCs were also obtained for the control group. Foll...
Source: Journal of Molecular Histology - March 18, 2015 Category: Laboratory Medicine Source Type: research

Acoustic Droplet Ejection Technology and Its Application in High-Throughput RNA Interference Screening
The development of acoustic droplet ejection (ADE) technology has resulted in many positive changes associated with the operations in a high-throughput screening (HTS) laboratory. Originally, this liquid transfer technology was used to simply transfer DMSO solutions of primarily compounds. With the introduction of Labcyte’s Echo 555, which has aqueous dispense capability, the application of this technology has been expanded beyond its original use. This includes the transfer of many biological reagents solubilized in aqueous buffers, including siRNAs. The Echo 555 is ideal for siRNA dispensing because it is accurate ...
Source: Journal of Laboratory Automation - January 20, 2016 Category: Laboratory Medicine Authors: Nebane, N. M., Coric, T., McKellip, S., Woods, L., Sosa, M., Rasmussen, L., Bjornsti, M.-A., White, E. L. Tags: Technology Briefs Source Type: research

HIF-1α Silencing Inhibits the Growth of Osteosarcoma Cells by Inducing Apoptosis.
Abstract BACKGROUND: Osteosarcoma (OS) is a malignant tumor of mesenchymal origin, which is generally locally aggressive and tends to produce early systemic metastases. Therefore, an identification of a novel therapeutic target is required. METHODS: We investigated the effect of small interfering RNA (siRNA) targeting hypoxia-inducible factor 1α (HIF-1α) on the growth of Osteosarcoma MG-63 and U2-OS cells. Two cell-lines were transfected with various concentrations of HIF-1α or control siRNA, and the effect on HIF-1α expression was analyzed by using quantitative polymerase chain reaction and western blot ...
Source: Annals of Clinical and Laboratory Science - February 29, 2016 Category: Laboratory Medicine Authors: Lv F, Du R, Shang W, Suo S, Yu D, Zhang J Tags: Ann Clin Lab Sci Source Type: research

Applications of RNA interference in the treatment of arthritis.
Abstract RNA interference (RNAi) is a cellular mechanism for post-transcriptional gene regulation mediated by small interfering RNA (siRNA) and microRNA. siRNA-based therapy holds significant promise for the treatment of a wide-range of arthritic diseases. siRNA selectively suppresses the expression of a gene product and can thus achieve the specificity that is lacking in small molecule inhibitors. The potential use of siRNA-based therapy in arthritis, however, has not progressed to clinical trials despite ample evidence for efficacy in preclinical studies. One of the main challenges to clinical translation is the...
Source: Translational Research : the journal of laboratory and clinical medicine - July 9, 2019 Category: Laboratory Medicine Authors: Rai MF, Pan H, Yan H, Sandell LJ, Pham CTN, Wickline SA Tags: Transl Res Source Type: research

Effect of Pim-3 Downregulation on Proliferation and Apoptosis in Lung Adenocarcinoma A549 Cells.
CONCLUSION: The downregulation of Pim-3 was closely related to the activation status of the lung STAT3 signaling pathway, mediated cell proliferation inhibition and induced apoptosis. PMID: 31882428 [PubMed - in process]
Source: Annals of Clinical and Laboratory Science - October 31, 2019 Category: Laboratory Medicine Authors: Fan X, Xie Y, Zhang L, Gao X, Han J, Chen Y, Yang J, Li S Tags: Ann Clin Lab Sci Source Type: research

Interleukin-18: A Novel Participant in the Occurrence, Development, and Drug Therapy of Obliterative Bronchiolitis Postlung Transplantation
CONCLUSIONS: IL-18 could be a novel molecular involved in the occurrence, development, and drug treatment of OB.PMID:34367377 | PMC:PMC8337162 | DOI:10.1155/2021/5586312
Source: Disease Markers - August 9, 2021 Category: Laboratory Medicine Authors: Ping Shu Wei Zhang Yanfei Zhang Yanfeng Zhao Yuping Li Xiaoqing Zhang Source Type: research