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Condition: Hypertension
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Total 587 results found since Jan 2013.
Small interfering RNA against ERK1/2 attenuates cigarette smoke-induced pulmonary vascular remodeling.
This study investigated the potential role of ERK1/2 in the proliferation of rat PASMCs (rPASMCs) and cigarette smoke-induced PVR in rats. A small interfering RNA (siRNA) against ERK1/2 (ERK1/2-siRNA) was synthesized, and it significantly reduced the expression of ERK1/2 and cyclin E1, significantly increased the proportion of cells arrested at G0/G1 phase and significantly suppressed the proliferation of rPASMCs treated with cigarette smoke extract compared with controls (all P<0.05). In rats, ERK1/2-siRNA, which was administered intranasally, also inhibited the activation of ERK1/2 and the upregulation of cyclin E1, b...
Source: Experimental and Therapeutic Medicine - December 6, 2017 Category: General Medicine Tags: Exp Ther Med Source Type: research
Central role of c-Src in NOX5- mediated redox signaling in vascular smooth muscle cells in human hypertension
CONCLUSIONS: We define NOX5/ROS/c-Src as a novel feedforward signaling network in human VSMCs. Amplification of this system in hypertension contributes to VSMC dysfunction. Dampening the NOX5/ROS/c-Src pathway may ameliorate hypertension-associated vascular injury.TRANSLATIONAL PERSPECTIVE: Oxidative stress is a major factor contributing to vascular damage in hypertension. We corroborate experimental evidence that NOX-derived ROS generation is increased in human vascular smooth muscle cells (VSMC) and demonstrate that in human hypertension NOX5 upregulation is a major trigger of VSMC dysfunction. We uncover new regulatory ...
Source: Cell Research - July 28, 2021 Category: Cytology Authors: Livia L Camargo Augusto C Montezano Misbah Hussain Yu Wang Zhiguo Zou Francisco J Rios Karla B Neves Rheure Alves-Lopes Fazli R Awan Tomasz J Guzik Thomas Jensen Richard C Hartley Rhian M Touyz Source Type: research
HMGB1 Inhibits HPAEC Migration Signal Transduction
This study focused on the effect of high mobility group box 1 protein (HMGB1) on these critical processes. HMGB1 had no effect on human pulmonary artery endothelial cell (HPAEC) proliferation. In contrast, treatment of HPAECs with HMGB1 dose-dependently inhibited VEGF-stimulated HPAEC migration. The effect of HMGB1 on HPAEC migration was TLR4-dependent because it was reversed by TLR4 siRNA or TLR4-neutralizing antibody. Exposure of HPAECs to hypoxia caused translocation and release of HMGB1 and inhibition of HPAEC migration. The effect of hypoxia on HPAEC migration was mediated by HMGB1 because HMGB1-neutralizing antibody ...
Source: Journal of Biological Chemistry - January 11, 2013 Category: Chemistry Authors: Bauer, E. M., Shapiro, R., Billiar, T. R., Bauer, P. M. Tags: Molecular Bases of Disease Source Type: research
Abstract 275: Tissue Transglutaminase Stabilizes Placental AT1 Receptor and Contributes to Pathophysiology in Preeclampsia Poster Session I
Preeclampsia (PE) is a serious hypertensive disease of pregnancy associated with the presence of the major angiotensin receptor AT1-agnostic autoantibodies (AT1-AAs) that cause the defining PE features when introduced into pregnant mice. To understand AT1-AA-mediated receptor activation in PE, we explored the role of tissue transglutaminases (TG2), the most ubiquitous member of a group of enzymes modifying proteins by catalyzing the formation of -(-glutamyl)-lysine isopeptide bonds. TG2, a versatile protein that can also function as a G protein, is enriched in the syncytiotrophoblasts of placenta, the key organ in PE patho...
Source: Hypertension - October 30, 2013 Category: Cardiology Authors: Liu, C., Wang, W., Parchim, N., Irani, R., Ning, C., Sun, K., Zhao, S., Zhao, C., Song, A., Zhang, Y., Blackwell, S., Sibai, B., Jin, J., Kellems, R., Xia, Y. Tags: Poster Session I Source Type: research
Abstract 280: Sflt-1 is Regulated via Both Transcriptional and Post-transcriptional Modification in Preeclampsia Poster Session I
Preeclampsia (PE) is a life-threatening hypertensive renal disorder of pregnancy. Soluble fms-like tyrosine kinase 1 (sFlt-1), an anti-angiogenic factor, is elevated and contributes to PE. However, molecular basis underlying its up-regulation in PE remains unclear. Here we report that hypoxia inducible factor-1α (HIF-1α), an important transcriptional factor, and U2AF65, a key molecule involved in alternative splicing, are significantly increased in the placentasof PE patients compared to the controls. Using two animal models of PE including angiotensin receptor type 1 receptor agonistic autoantibody (AT1-AA) an...
Source: Hypertension - October 30, 2013 Category: Cardiology Authors: Wang, W., Parchim, N., Iriyama, T., Luo, R., Zhao, C., Zhang, Y., Blackwell, S., Sabai, B., Tao, L., Kellems, R., Xia, Y. Tags: Poster Session I Source Type: research
Downregulation of L-type Ca2+ channel in rat mesenteric arteries leads to loss of smooth muscle contractile phenotype and inward hypertrophic remodeling
L-type Ca2+ channels (LTCCs) are important for vascular smooth muscle cell (VSMC) contraction, as well as VSMC differentiation, as indicated by loss of LTCCs during VSMC dedifferentiation. However, it is not clear whether loss of LTCCs is a primary event underlying phenotypic modulation or whether loss of LTCCs has significance for vascular structure. We used small interference RNA (siRNA) transfection in vivo to investigate the role of LTCCs in VSMC phenotypic expression and structure of rat mesenteric arteries. siRNA reduced LTCC mRNA and protein expression in rat mesenteric arteries 3 days after siRNA transfection to 12...
Source: AJP: Heart and Circulatory Physiology - May 1, 2014 Category: Cardiology Authors: Kudryavtseva, O., Herum, K. M., Dam, V. S., Straarup, M. S., Kamaev, D., Briggs Boedtkjer, D. M., Matchkov, V. V., Aalkjaer, C. Tags: VASCULAR BIOLOGY AND MICROCIRCULATION Source Type: research
Abstract P099: Angiotensin Type I Receptor (AT1R) and (Pro)renin Receptor (PRR) Functional Heterodimer Mediates ERK Phosphorylation Session Title: Poster Session 1- Trainee Onsite Poster Competition and Reception
Receptor dimerization was shown to enhance the receptor efficacy, trafficking and signal transduction. Angiotensin II type 1 receptor (AT1R) and (Pro)renin receptor (PRR) are expressed in the kidney. Studies demonstrated that these two receptors have some similarities in their signaling mechanisms and effects. In the present study we hypothesized that the AT1R and PRR form functional heterodimer to enhance cellular ERK phosphorylation. Using Immunoprecipitation technique, confocal immunofluorescence staining (IF), and fluorescence resonance energy transfer (FRET), we evaluated the presence of AT1R and PRR heterodimer in ra...
Source: Hypertension - November 3, 2015 Category: Cardiology Authors: Quadri, S. S. A., Li, C., Culver, S., Siragy, H. M. Tags: Session Title: Poster Session 1- Trainee Onsite Poster Competition and Reception Source Type: research
Abstract P141: Cardiac TRH Partly Mediates Angiotensin II-induced Fibrotic and Hypertrophy Effects in "in vivo" and "in vitro" Models Session Title: Angiotensin Action and Signaling/Renin Angiotensin System 1
Cardiac TRH (cTRH) is overexpressed in the hypertrophied ventricle (LV) of the SHR. Additionally in vivo siRNA-TRH treatment induced downregulation of LV-TRH preventing cardiac hypertrophy and fibrosis demonstrating that TRH is involved in hypertrophic and fibrotic processes. Moreover, in a normal heart, the increase of LV TRH expression alone could induce structural changes where fibrosis and hypertrophy could be involved, independently of any other system alterations.Is well-known the cardiac hypertrophy/ fibrotic effects induced by AII, raising the question of whether specific LV cTRH inhibition might attenuates AII ind...
Source: Hypertension - November 3, 2015 Category: Cardiology Authors: Garcia, S. I., Peres Diaz, L. S., Aisicovich, M., Schuman, M. L., Landa, M. S. Tags: Session Title: Angiotensin Action and Signaling/Renin Angiotensin System 1 Source Type: research
Upregulation of SRF Is Associated with Hypoxic Pulmonary Hypertension by Promoting Viability of Smooth Muscle Cells via Increasing Expression of Bcl ‐2
This article is protected by copyright. All rights reserved
Source: Journal of Cellular Biochemistry - February 7, 2017 Category: Biochemistry Authors: Xing Ding, Sijing Zhou, Min Li, Chao Cao, Peipei Wu, Li Sun, Guanghe Fei, Ran Wang Tags: Article Source Type: research
Effects of small interfering RNA targeting TLR4 on expressions of adipocytokines in obstructive sleep apnea hyponea syndrome with hypertension in a rat model
This article is protected by copyright. All rights reserved
Source: Journal of Cellular Physiology - December 7, 2017 Category: Cytology Authors: Jing ‐Guo Wu, Nan Xun, Li‐Jin Zeng, Zhen‐Yu Li, Yan‐Bing Liang, Hao Tang, Zhong‐Fu Ma Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Potential Role of Cartilage Oligomeric Matrix Protein in the Modulation of Pulmonary Arterial Smooth Muscle Superoxide by Hypoxia.
Abstract
Changes in reactive oxygen species and extracellular matrix seem to participate in pulmonary hypertension development. Since we recently reported evidence for chronic hypoxia decreasing expression of cartilage oligomeric matrix protein (COMP) and evidence for this controlling loss of pulmonary arterial smooth muscle BMP receptor-2 (BMPR2) and contractile phenotype proteins, we examined if changes in superoxide metabolism could be an important factor in a bovine pulmonary artery (BPA), organoid cultured under hypoxia for 48hrs model. Hypoxia (3% oxygen) caused a depletion of COMP in BPA, but not in bovine ...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - August 6, 2019 Category: Cytology Authors: Yu H, Alruwaili N, Hu B, Kelly MR, Zhang B, Sun D, Wolin MS Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research
Abstract 75: Nrf2 Mediates the Antioxidant Effect of D2r Via Dj-1 in the Kidney Oral Session IX: Concurrent B Oxidative Stress
Renal dopamine D2 receptor (D2R) dysfunction is associated with increased oxidative stress and high blood pressure. We have reported that DJ-1 is regulated by D2R in the kidney. Nrf2 is a transcription factor that regulates the expression of several antioxidant genes. We hypothesize that Nrf2 is involved in the DJ-1-mediated regulation of reactive oxygen species (ROS) production in the kidney. DJ-1 and Nrf2 co-inmunoprecipitate in mouse kidney. Selective renal silencing of D2R in mice by the renal subcapsular infusion of D2R siRNA (~50%) decreases Nrf2 expression (63.4%±3.4, n=7, P<0.05), and silencing of D2R exp...
Source: Hypertension - October 30, 2013 Category: Cardiology Authors: Cuevas, S., Yang, Y., Assico, L., Feranil, J., Jones, J., Armando, I., Jose, P. A. Tags: Oral Session IX: Concurrent B Oxidative Stress Source Type: research
Abstract 191: Transcriptional Regulation of Renin by Nuclear Receptors Co-regulated With Renin Poster Session I
Expression of the renin gene is required to maintain normal morphological and physiological identity of renal juxtaglomerular (JG) cells, yet the mechanisms regulating renin gene transcription remain elusive. We re-examined data from Brunskill et. al (JASN 22:2213, 2011), investigating genome-wide gene expression in JG and other renal cell types. Based on our previous data implicating nuclear receptors (RAR, RXR, VDR, PPARG, Nr2f2 and Nr2f6) in the regulation of mouse and human renin gene expression, we focused our analysis on the expression of genes encoding the 48 nuclear hormone receptors and their co-regulation with re...
Source: Hypertension - October 30, 2013 Category: Cardiology Authors: Lu, K.-T., Weatherford, E. T., Ketsawatsomkron, P., Grobe, J. L., Sigmund, C. D. Tags: Poster Session I Source Type: research
Altered Pulmonary Artery Endothelial-Smooth Muscle Cell Interactions in Experimental Congenital Diaphragmatic Hernia.
ConclusionsPAEC dysfunction in experimental CDH increases SMC proliferation via ET-1 induced ROS production by PAEC.Pediatric Research (2015); doi:10.1038/pr.2015.13.
PMID: 25580737 [PubMed - as supplied by publisher]
Source: Pediatric Research - January 12, 2015 Category: Pediatrics Authors: Acker SN, Seedorf GJ, Abman SH, Nozik-Grayck E, Kuhn K, Partrick DA, Gien J Tags: Pediatr Res Source Type: research
Role of brain aldosterone and mineralocorticoid receptors in aldosterone-salt hypertension in rats
We examined the role of the subfornical organ (SFO), aldo synthesized locally in the brain, and MR and AT1R specifically in the paraventricular nucleus (PVN) in aldo-salt hypertension. Wistar rats were treated with subcutaneous aldo (1μg/h) plus saline as drinking fluid, and gene expression was assessed by real-time qPCR. Other sets of rats received chronic intra-cerebroventricular (icv) infusion of aldo synthase (AS) inhibitor FAD286, MR blocker eplerenone or vehicle, electrolytic or sham lesions of the SFO, or intra-PVN infusion of AAV-MR-siRNA or AAV-AT1aR-siRNA. Infusion of aldo had no effect on 11βHSD2, MR and AT1R ...
Source: Neuroscience - December 15, 2015 Category: Neuroscience Source Type: research
