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Infectious Disease: MERS

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Total 28 results found since Jan 2013.

siRNA as a potential therapy for COVID-19
Curr Drug Deliv. 2021 Aug 5. doi: 10.2174/1567201818666210805145320. Online ahead of print.ABSTRACTSevere Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV2) is a highly contagious virus causing COVID-19 disease that severely impacted the world health, education, and economy systems in 2020. The numbers of infection cases and reported deaths are still increasing with no specific treatment identified yet to halt this pandemic. Currently, several proposed treatments are under preclinical and clinical investigations now, alongside the race to vaccinate to as much individuals as possible. The genome of SARS-CoV2 share similar...
Source: Current Drug Delivery - August 6, 2021 Category: Drugs & Pharmacology Authors: Ahmad Aljaberi Eman M Migdadi Khalid M Abu Khadra Mahmoud Abu Samak Iman A Basheti Nizar Al-Zoubi Source Type: research

Molecules, Vol. 26, Pages 2610: In Vitro Inhibitory Analysis of Rationally Designed siRNAs against MERS-CoV Replication in Huh7 Cells
In this report, we have discussed the prediction, designing, and evaluation of potential siRNA targeting the ORF1ab gene for the inhibition of MERS-CoV replication. The online software, siDirect 2.0 was used to predict and design the siRNAs, their secondary structure and their target accessibility. ORF1ab gene folding was performed by RNAxs and RNAfold software. A total of twenty-one siRNAs were selected from 462 siRNAs according to their scoring and specificity. siRNAs were evaluated in vitro for their cytotoxicity and antiviral efficacy in Huh7 cell line. No significant cytotoxicity was observed for all siRNAs in Huh7 ce...
Source: Molecules - April 29, 2021 Category: Chemistry Authors: Sherif Aly El-Kafrawy Sayed Sartaj Sohrab Zeenat Mirza Ahmed M. Hassan Fatima Alsaqaf Esam Ibraheem Azhar Tags: Article Source Type: research

Lessons from SARS and MERS remind us of the possible therapeutic effects of implementing a siRNA strategy to target COVID-19: Shoot the messenger!
PMID: 32677763 [PubMed - as supplied by publisher]
Source: J Cell Mol Med - July 16, 2020 Category: Molecular Biology Authors: Habtemariam S, Berindan-Neagoe I, Cismaru CA, Schaafsma D, Nabavi SF, Ghavami S, Banach M, Nabavi SM Tags: J Cell Mol Med Source Type: research

Viruses, Vol. 11, Pages 152: Interferon Regulatory Factor 3-Mediated Signaling Limits Middle-East Respiratory Syndrome (MERS) Coronavirus Propagation in Cells from an Insectivorous Bat
ram Misra Insectivorous bats are speculated to be ancestral hosts of Middle-East respiratory syndrome (MERS) coronavirus (CoV). MERS-CoV causes disease in humans with thirty-five percent fatality, and has evolved proteins that counteract human antiviral responses. Since bats experimentally infected with MERS-CoV do not develop signs of disease, we tested the hypothesis that MERS-CoV would replicate less efficiently in bat cells than in human cells because of its inability to subvert antiviral responses in bat cells. We infected human and bat (Eptesicus fuscus) cells with MERS-CoV and observed that the virus grew to hig...
Source: Viruses - February 13, 2019 Category: Virology Authors: Arinjay Banerjee Darryl Falzarano Noreen Rapin Jocelyne Lew Vikram Misra Tags: Article Source Type: research

A recombinant VSV-vectored MERS-CoV vaccine induces neutralizing antibody and T cell responses in rhesus monkeys after single dose immunization
Publication date: Available online 12 December 2017 Source:Antiviral Research Author(s): Renqiang Liu, Jinliang Wang, Yu Shao, Xijun Wang, Huilei Zhang, Lei Shuai, Jinying Ge, Zhiyuan Wen, Zhigao Bu Middle East respiratory syndrome coronavirus (MERS-CoV) has been a highly threatening zoonotic pathogen since its outbreak in 2012. Similar to SARS-CoV, MERS-CoV belongs to the coronavirus family and can induce severe respiratory symptoms in humans, with an average case fatality rate of 35% according to the World Health Organization. Spike (S) protein of MERS-CoV is immunogenic and can induce neutralizing antibodies, thus is a...
Source: Antiviral Therapy - December 13, 2017 Category: Virology Source Type: research

A recombinant VSV-vectored MERS-CoV vaccine induces neutralizing antibody and T cell responses in rhesus monkeys after single dose immunization.
Abstract Middle East respiratory syndrome coronavirus (MERS-CoV) has been a highly threatening zoonotic pathogen since its outbreak in 2012. Similar to SARS-CoV, MERS-CoV belongs to the coronavirus family and can induce severe respiratory symptoms in humans, with an average case fatality rate of 35% according to the World Health Organization. Spike (S) protein of MERS-CoV is immunogenic and can induce neutralizing antibodies, thus is a potential major target for vaccine development. Here we constructed a chimeric virus based on the vesicular stomatitis virus (VSV) in which the G gene was replaced by MERS-CoV S gen...
Source: Antiviral Research - December 12, 2017 Category: Virology Authors: Liu R, Wang J, Shao Y, Wang X, Zhang H, Shuai L, Ge J, Wen Z, Bu Z Tags: Antiviral Res Source Type: research

Design and delivery of therapeutic siRNAs: application to MERS-Coronavirus.
CONCLUSION: The efficacy of siRNA-based therapeutics has been used not only against many viral diseases but also against non-viral diseases, cancer, dominant genetic disorders, and autoimmune disease. This innovative technology has attracted researchers, academia and pharmaceuticals industries towards designing and development of highly effective and targeted disease therapy. By using this technology, an effective and potential siRNAs can be designed, delivered and their efficacy with toxic effects and immunogenic responses can be tested against MERS-CoV. PMID: 29119921 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Design - November 8, 2017 Category: Drugs & Pharmacology Authors: Sohrab SS, El-Kafrawy SA, Mirza Z, Kamal MA, Azhar EI Tags: Curr Pharm Des Source Type: research

Regulation of Chromatin Assembly and Cell Transformation by Formaldehyde Exposure in Human Cells
Conclusions: We propose that the inhibition of chromatin assembly represents a novel mechanism of cell transformation induced by the environmental and occupational chemical carcinogen FA. https://doi.org/10.1289/EHP1275 Received: 25 October 2016 Revised: 19 May 2017 Accepted: 23 May 2017 Published: 21 September 2017 Address correspondence to C. Jin, Dept. of Environmental Medicine, New York University School of Medicine, 57 Old Forge Rd., Tuxedo Park, NY 10987 USA. Telephone: (845) 731-3602. Email: Chunyuan.jin@nyumc.org *Current affiliation: Medical School of Nanjing University, Nanjing, China. †Current affiliatio...
Source: EHP Research - September 21, 2017 Category: Environmental Health Authors: Daniil Lyalko Tags: Research Source Type: research

Middle east respiratory syndrome corona virus spike glycoprotein suppresses macrophage responses via DPP4-mediated induction of IRAK-M and PPAR γ.
Middle east respiratory syndrome corona virus spike glycoprotein suppresses macrophage responses via DPP4-mediated induction of IRAK-M and PPARγ. Oncotarget. 2017 Jan 19;: Authors: Al-Qahtani AA, Lyroni K, Aznaourova M, Tseliou M, Al-Anazi MR, Al-Ahdal MN, Alkahtani S, Sourvinos G, Tsatsanis C Abstract Middle East Respiratory Syndrome Corona Virus (MERS-CoV) is transmitted via the respiratory tract and causes severe Acute Respiratory Distress Syndrome by infecting lung epithelial cells and macrophages. Macrophages can readily recognize the virus and eliminate it. MERS-CoV infects cells via its Spike (...
Source: Oncotarget - January 25, 2017 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

GSE73422 Expression data from human osteosarcoma cells treated with CDK11 siRNA
Contributors : Yong Feng ; Zhenfeng DuanSeries Type : Expression profiling by arrayOrganism : Homo sapiensThe U-2OS and KHOS osteosarcoma cell lines where seeded onto the 100mm cell culture plate, then treated with CDK11 siRNA (40nM) or nonspecificsiRNA (40nM). Change with regular medium 24 hour later. Total RNA was collected from these cells using TRIzol® Reagent (GIBCO Grand Island, NY) according to the manufacturer’s instructions. To account for and eliminate biologic noise, RNA was isolated from three distinct flasks of each cell line and pooled together. RNA quality was determined via ethidium bromide staining foll...
Source: GEO: Gene Expression Omnibus - October 7, 2015 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research