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Infectious Disease: Chikungunya

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Total 22 results found since Jan 2013.

Molecules, Vol. 27, Pages 1170: Effect of Cationic Lipid Nanoparticle Loaded siRNA with Stearylamine against Chikungunya Virus
In this study, nanodelivery systems (hybrid polymeric/solid lipid nanoparticles) using cationic lipids (stearylamine, C9 lipid, and dioctadecylamine) and polymers (branched PEI-g-PEG -PEG) were prepared, characterized, and complexed with siRNA. The four developed delivery systems (F1,F2,F3, and F4) were assessed for stability and potential toxicities against CHIKV. In comparison to the other nanodelivery systems, F4 containing stearylamine (Octadecylamine; ODA), with an induced optimum cationic charge of 45.7 mV in the range of 152.1 nm, allowed maximum siRNA complexation, better stability, and higher transfection, with st...
Source: Molecules - February 9, 2022 Category: Chemistry Authors: Manish Kumar Jeengar Mallesh Kurakula Poonam Patil Ashwini More Ramakrishna Sistla Deepti Parashar Tags: Article Source Type: research

GSE65070 The hub protein Loqs connects the miRNA and endo-siRNA pathways in mosquitos
Contributors : Mary E Haac ; Michelle A Anderson ; Heaher L Eggleston ; Kevin M Myles ; Zach N AdelmanSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Aedes aegyptiAedes aegypti mosquitoes vector several arboviruses of global health significance, including dengue viruses and chikungunya virus. RNA interference (RNAi) plays an important role in antiviral immunity, gene regulation and protection from transposable elements. Double-stranded RNA binding proteins (dsRBPs) are important for efficient RNAi; in Drosophila functional specialization of the miRNA, endo-siRNA and exo-siRNA pathway is aided...
Source: GEO: Gene Expression Omnibus - April 8, 2015 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Aedes aegypti Source Type: research

The < i > Aedes aegypti < /i > siRNA pathway mediates broad-spectrum defense against human pathogenic viruses and modulates antibacterial and antifungal defenses
by Yuemei Dong, Shengzhang Dong, Nahid Borhani Dizaji, Natalie Rutkowski, Tyler Pohlenz, Kevin Myles, George Dimopoulos The mosquito’s innate immune system defends against a variety of pathogens, and the conserved siRNA pathway plays a central role in the control of viral infections. Here, we show that transgenic overexpression of Dicer2 (Dcr2) orR2d2 resulted in an accumulation of 21-nucleotide viral sequences that was accompanied by a significant suppression of dengue virus (DENV), Zika virus (ZIKV), and chikungunya virus (CHIKV) replication, thus indicating the broad-spectrum antiviral response mediated by the siRNA ...
Source: PLoS Biology: Archived Table of Contents - June 9, 2022 Category: Biology Authors: Yuemei Dong Source Type: research

The hub protein loquacious connects the microRNA and short interfering RNA pathways in mosquitoes
Aedes aegypti mosquitoes vector several arboviruses of global health significance, including dengue viruses and chikungunya virus. RNA interference (RNAi) plays an important role in antiviral immunity, gene regulation and protection from transposable elements. Double-stranded RNA binding proteins (dsRBPs) are important for efficient RNAi; in Drosophila functional specialization of the miRNA, endo-siRNA and exo-siRNA pathway is aided by the dsRBPs Loquacious (Loqs-PB, Loqs-PD) and R2D2, respectively. However, this functional specialization has not been investigated in other dipterans. We were unable to detect Loqs-PD in Ae....
Source: Nucleic Acids Research - April 19, 2015 Category: Research Authors: Haac, M. E., Anderson, M. A. E., Eggleston, H., Myles, K. M., Adelman, Z. N. Tags: Molecular Biology Source Type: research

Viruses, Vol. 10, Pages 88: Spindle-E Acts Antivirally Against Alphaviruses in Mosquito Cells
er Schnettler Mosquitoes transmit several human- and animal-pathogenic alphaviruses (Togaviridae family). In alphavirus-infected mosquito cells two different types of virus-specific small RNAs are produced as part of the RNA interference response: short-interfering (si)RNAs and PIWI-interacting (pi)RNAs. The siRNA pathway is generally thought to be the main antiviral pathway. Although an antiviral activity has been suggested for the piRNA pathway its role in host defences is not clear. Knock down of key proteins of the piRNA pathway (Ago3 and Piwi5) in Aedes aegypti-derived cells reduced the production of alphavirus ch...
Source: Viruses - February 18, 2018 Category: Virology Authors: Margus Varjak Isabelle Dietrich Vattipally B. Sreenu Bethan Eluned Till Andres Merits Alain Kohl Esther Schnettler Tags: Article Source Type: research

Induction of RNA interference to block Zika virus replication and transmission in the mosquito Aedes aegypti.
This study lays critical groundwork for pursuing ZIKV transmission-blocking strategies that exploit the Ae. aegypti exo-siRNA response for arbovirus suppression in natural populations. PMID: 31103782 [PubMed - as supplied by publisher]
Source: Insect Biochemistry and Molecular Biology - May 15, 2019 Category: Biochemistry Authors: Magalhaes T, Bergren NA, Bennett SL, Borland EM, Hartman DA, Lymperopoulos K, Sayre R, Borlee BR, Campbell CL, Foy BD, Olson KE, Blair CD, Black W, Kading RC Tags: Insect Biochem Mol Biol Source Type: research

Regulation of the chikungunya-virus-induced innate inflammatory response by protein tyrosine phosphatase non-receptor 6 in muscle cells
AbstractChikungunya virus (CHIKV)-induced myositis is an emerging affliction with high incidence globally. Given the essential regulatory role of protein tyrosine phosphatase non-receptor 6 (PTPN6) in virus-induced myositis, the expression of the PTPN6 and TNF- α genes in a CHIKV-infected muscle cell line was examined by quantitative PCR, and the expression of PTPN6 and STAT 3 was examined by immunoblotting. In addition, the effect of PTPN6 siRNA treatment on TNF-α gene expression was assessed. Increased higher expression of PTPN6 and TNF-α, and signifi cant upregulation of TNF-α upon PTPN6 siRNA treatment were observe...
Source: Archives of Virology - January 1, 2018 Category: Virology Source Type: research

Host ESCRT factors are recruited during chikungunya virus infection and are required for the intracellular viral replication cycle Cell Biology
Chikungunya fever is a re-emerging zoonotic disease caused by chikungunya virus (CHIKV), a member of the Alphavirus genus in the Togaviridae family. Only a few studies have reported on the host factors required for intracellular CHIKV trafficking. Here, we conducted an imaging-based siRNA screen to identify human host factors for intracellular trafficking that are involved in CHIKV infection, examined their interactions with CHIKV proteins, and investigated the contributions of these proteins to CHIKV infection. The results of the siRNA screen revealed that host endosomal sorting complexes required for transport (ESCRT) pr...
Source: Journal of Biological Chemistry - June 4, 2020 Category: Chemistry Authors: Shiho Torii, Yasuko Orba, Michihito Sasaki, Koshiro Tabata, Yuji Wada, Michael Carr, Jody Hobson-Peters, Roy A. Hall, Ayato Takada, Takasuke Fukuhara, Yoshiharu Matsuura, William W. Hall, Hirofumi Sawa Tags: Microbiology Source Type: research

Chikungunya virus nsP3 & nsP4 interacts with HSP-90 to promote virus replication: HSP-90 inhibitors reduce CHIKV infection and inflammation in vivo.
In this study, using biochemical pull-downs, mass-spectrometry, and microscopic imaging techniques, we have identified novel interactions between CHIKV nsP3 or nsP4 proteins with the host stress-pathway chaperone HSP-90 protein. Indeed, silencing of HSP-90 transcripts using siRNA disrupts CHIKV replication in cultured cells. Furthermore, drugs targeting HSP-90, such as commercially available geldanamycin, as well as other specific HSP-90 inhibitor drugs that had been obtained from a purinome mining approach (HS-10 and SNX-2112) showed dramatic reduction in viral titers and reduced inflammation in a CHIKV mouse model of sev...
Source: Antiviral Research - December 31, 2013 Category: Virology Authors: Rathore AP, Haystead T, Das PK, Merits A, Ng ML, Vasudevan SG Tags: Antiviral Res Source Type: research

Proteomic Profiling of Chikungunya Virus-Infected Human Muscle Cells: Reveal The Role of Cytoskeleton Network in CHIKV Replication.
Abstract Chikungunya virus (CHIKV) is an arthropod-borne, positive-sense, single-stranded RNA virus belonging to genus Alphavirus and family Togaviridae. The clinical manifestations developed upon CHIKV-infection include fever, myositis, arthralgia and maculopapular rash. Thus, the re-emergence of CHIKV has posed serious health threats worldwide. Due to the fact that myositis is induced upon CHIKV-infection, we sought to understand the dynamic proteomic regulation in SJCRH30, a human rhabdomyosarcoma cell line, to gain insights on CHIKV pathogenesis. Two-dimensional gel electrophoresis (2DE) in combination of matr...
Source: Journal of Proteomics - June 13, 2014 Category: Biochemistry Authors: Issac TH, Lee TE, Chu JJ Tags: J Proteomics Source Type: research

Proteomics Profiling of Chikungunya-Infected Aedes albopictus C6/36 Cells Reveal Important Mosquito Cell Factors in Virus Replication
In this study, the CHIKV-infected C6/36 cells with differential cellular proteins expression were profiled using two-dimensional gel electrophoresis (2DE) coupled with the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). 2DE analysis on CHIKV-infected C6/36 cells has shown 23 mosquito cellular proteins that are differentially regulated, and which are involved diverse biological pathways, such as protein folding and metabolic processes. Among those identified mosquito proteins, spermatogenesis-associated factor, enolase phosphatase e-1 and chaperonin-60kD have been found to...
Source: PLoS Neglected Tropical Diseases - March 4, 2015 Category: Tropical Medicine Authors: Regina Ching Hua Lee et al. Source Type: research

Inflammasome signaling pathways exert antiviral effect against Chikungunya virus in human dermal fibroblasts.
Abstract Arboviruses represent an emerging threat to human. They are transmitted to vertebrates by the bite of infected arthropods. Early transmission to vertebrates is initiated by skin puncture and deposition of virus in this organ. However, events at the bite site remain largely unknown. Here, we report that Chikungunya virus (CHIKV) and West Nile virus (WNV), despite belonging to distinct viral families, elicit a common antiviral signature in primary human dermal fibroblasts, attesting for the up regulation of interferon signalling pathways and leading to an increased expression of IFN-β, interleukins and che...
Source: Infection, Genetics and Evolution - April 3, 2015 Category: Genetics & Stem Cells Authors: Ekchariyawat P, Hamel R, Bernard E, Wichit S, Surasombatpattana P, Talignani L, Thomas F, Choumet V, Yssel H, Desprès P, Briant L, Missé D Tags: Infect Genet Evol Source Type: research

GSE69980 Exclusion of non-expressed genes from the Chikungunya RNAi screen hit identification process
Contributors : Alexander Karlas ; Thomas Meyer ; Hans-Joachim MollenkopfSeries Type : Expression profiling by arrayOrganism : Homo sapiensTo allow exclusion of non-expressed genes from the hit identification process, microarray analyses of (i) Allstars transfected and Chikungunya (CHIKV) infected, (ii) Allstars transfected and non-infected, (iii) non-transfected and CHIKV infected and (iv) non-transfected and non-infected 293 cells were performed. Transfection was done by adding 4x10e5 293 cells (in complete DMEM medium) to a mixture of serum-free RPMI medium, HiperFect (Qiagen) and Allstars siRNAs (Qiagen, final siRNA con...
Source: GEO: Gene Expression Omnibus - May 9, 2016 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

Viruses, Vol. 11, Pages 540: Assessing the Potential Interactions between Cellular miRNA and Arboviral Genomic RNA in the Yellow Fever Mosquito, Aedes aegypti
Failloux Although the role of exogenous small interfering RNA (siRNA) and P-element induced wimpy testis (PIWI)-interacting RNA (piRNA) pathways in mosquito antiviral immunity is increasingly better understood, there is still little knowledge regarding the role of mosquito cellular microRNA (miRNA). Identifying direct interactions between the mosquito miRNAs and the RNA genome of arboviruses and choosing the relevant miRNA candidates to explore resulting antiviral mechanisms are critical. Here, we carried out genomic analyses to identify Aedes aegypti miRNAs that potentially interact with various lineages and genotyp...
Source: Viruses - June 9, 2019 Category: Virology Authors: Yen Chen Sreenu Kohl Failloux Tags: Article Source Type: research

Macropinocytosis Dependent Entry of Chikungunya Virus into Human Muscle Cells
This study shows for the first time, that the infectious entry of CHIKV into human muscle cells is mediated by macropinocytosis. Together, the da ta from this study may pave the way for the development of specific inhibitors that target the entry process of CHIKV into cells.
Source: PLoS Neglected Tropical Diseases - August 25, 2019 Category: Tropical Medicine Authors: Ching Hua, Regina Lee Source Type: research