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Source: Journal of Cellular and Molecular Medicine

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Total 94 results found since Jan 2013.

Otx2 enhances transdifferentiation of M üller cells‐derived retinal stem cells into photoreceptor‐like cells
In conclusion, Otx2 enhances transdifferentiation of MC‐RSCs into photoreceptor‐like cells and this is associated with the inhibition of Wnt signalling. Otx2 is a potential target f or gene therapy of retinal degenerative diseases.
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Yu Xiong, Hongpei Ji, Zhipeng You, Fei Yao, Rongrong Zhou, Weitao Song, Xiaobo Xia Tags: ORIGINAL ARTICLE Source Type: research

Effect of CLIC1 gene silencing on proliferation, migration, invasion and apoptosis of human gallbladder cancer cells
This study aimed to explore the effects of CLIC1 gene silencing on proliferation, migration, invasion and apoptosis of human gallbladder cancer (GBC). GBC and normal gallbladder tissues were extracted for the detection of mRNA and protein expressions of CLIC1. GBC‐SD and NOZ cells in the logarithmic growth phase were selected to conduct the experiment. Three different siRNA recombined expression vectors were established using CLIC1 as a target at different sites. Reverse transcription quantitative polymerase chain reaction (RT‐qPCR) and Western blotting were, respectively, used to detect the CLIC1 mRNA and protein expr...
Source: Journal of Cellular and Molecular Medicine - March 8, 2018 Category: Molecular Biology Authors: Yue ‐Ming He, Zhong‐Lin Zhang, Quan‐Yan Liu, Yu‐Sha Xiao, Lei Wei, Chen Xi, Xiang Nan Tags: Original Article Source Type: research

HMQ ‐T‐F2 exert antitumour effects by upregulation of Axin in human cervical HeLa cells
In this study, we reported that HMQ‐T‐F2(F2) significantly inhibited cell proliferation and transplantable tumour growth. Mechanistically, HMQ‐T‐F2 inhibited HeLa cell growth through repressing the expression and nuclear translocation of β‐catenin, enhancing Axin expression, as well as downregulating the Wnt downstream targeted proteins. Knock‐down of a checkpoint β‐catenin by siRNA significantly attenuated HeLa cell proliferation. Furthermore, XAV939, an inhibitor of β‐catenin, was used to treat HeLa cells and the results demonstrated that HMQ‐T‐F2 inhibited proliferation and migration via the inhib...
Source: Journal of Cellular and Molecular Medicine - March 7, 2018 Category: Molecular Biology Authors: Bingling Dai, Tianfeng Yang, Yujiao Ma, Nan Ma, Xianpeng Shi, Dongdong Zhang, Jie Zhang, Yanmin Zhang Tags: SHORT COMMUNICATION Source Type: research

Qiliqiangxin attenuates hypoxia ‐induced injury in primary rat cardiac microvascular endothelial cells via promoting HIF‐1α‐dependent glycolysis
In this study, we investigated the effects of qiliqiangxin (QL) on primary rat CMECs exposed to hypoxia and the underlying mechanisms. Rat CMECs were successfully isolated and passaged to the second generation. CMECs that were pre‐treated with QL (0.5 mg/mL) and/or HIF‐1α siRNA were cultured in a three‐gas hypoxic incubator chamber (5% CO2, 1% O2, 94% N2) for 12 hours. Firstly, we demonstrated that compared with hypoxia group, QL effectively promoted the proliferation while attenuated the apoptosis, improved mitochondrial function and reduced ROS generation in hypoxic CMECs in a HIF‐1α‐dependent manner. Meanw...
Source: Journal of Cellular and Molecular Medicine - March 4, 2018 Category: Molecular Biology Authors: Yanyan Wang, Xueting Han, Mingqiang Fu, Jingfeng Wang, Yu Song, Yuan Liu, Jingjing Zhang, Jingmin Zhou, Junbo Ge Tags: ORIGINAL ARTICLE Source Type: research

Down ‐regulation of PKM2 enhances anticancer efficiency of THP on bladder cancer
This study aims to investigate the effect of down‐regulation of PKM2 on THP efficiency. Via inhibitor or siRNA, the effects of reduced PKM2 on the efficiency of THP were determined in 2 human and 1 murine bladder cancer cell lines, using MTT, cologenic and fluorescence approaches. Molecular mechanisms of PKM2 on THP sensitization were explored by probing p‐AMPK and p‐STAT3 levels via WB. Syngeneic orthotopic bladder tumour model was applied to evaluate this efficiency in vivo, analysed by Kaplan‐Meier survival curves, body and bladder weights plus immunohistochemistric tumour biomarkers. PKM2 was overexpressed in b...
Source: Journal of Cellular and Molecular Medicine - March 1, 2018 Category: Molecular Biology Authors: Qiongli Su, Ting Tao, Lei Tang, Jun Deng, Kwame Oteng Darko, Sichun Zhou, Mei Peng, Shanping He, Qing Zeng, Alex F. Chen, Xiaoping Yang Tags: ORIGINAL ARTICLE Source Type: research

Caveolin ‐1 down‐regulation is required for Wnt5a‐Frizzled 2 signalling in Ha‐RasV12‐induced cell transformation
Abstract Caveolin‐1 (Cav1) is down‐regulated during MK4 (MDCK cells harbouring inducible Ha‐RasV12 gene) transformation by Ha‐RasV12. Cav1 overexpression abrogates the Ha‐RasV12‐driven transformation of MK4 cells; however, the targeted down‐regulation of Cav1 is not sufficient to mimic this transformation. Cav1‐silenced cells, including MK4/shCav1 cells and MDCK/shCav1 cells, showed an increased cell area and discontinuous junction‐related proteins staining. Cellular and mechanical transformations were completed when MDCK/shCav1 cells were treated with medium conditioned by MK4 cells treated with IPTG (MK...
Source: Journal of Cellular and Molecular Medicine - March 1, 2018 Category: Molecular Biology Authors: Hsiu ‐Kuan Lin, Hsi‐Hui Lin, Yu‐Wei Chiou, Ching‐Lung Wu, Wen‐Tai Chiu, Ming‐Jer Tang Tags: ORIGINAL ARTICLE Source Type: research

SIRT1/FoxO3 axis alteration leads to aberrant immune responses in bronchial epithelial cells
In conclusion, CSE impairs the function of SIRT1/FoxO3 axis in bronchial epithelium, dysregulating NF‐κB activity and inducing pro‐inflammatory responses.
Source: Journal of Cellular and Molecular Medicine - February 7, 2018 Category: Molecular Biology Authors: Serena Di Vincenzo, Irene H. Heijink, Jacobien A. Noordhoek, Chiara Cipollina, Liboria Siena, Andreina Bruno, Maria Ferraro, Dirkje S. Postma, Mark Gjomarkaj, Elisabetta Pace Tags: Original Article Source Type: research

Overexpression of geranylgeranyl diphosphate synthase contributes to tumour metastasis and correlates with poor prognosis of lung adenocarcinoma
This study aimed to evaluate the biological role of geranylgeranyl diphosphate synthase (GGPPS) in the progression of lung adenocarcinoma. GGPPS expression was detected in lung adenocarcinoma tissues by qRT‐PCR, tissue microarray (TMA) and western blotting. The relationships between GGPPS expression and the clinicopathological characteristics and prognosis of lung adenocarcinoma patients were assessed. GGPPS was down‐regulated in SPCA‐1, PC9 and A549 cells using siRNA and up‐regulated in A549 cells using an adenoviral vector. The biological roles of GGPPS in cell proliferation, apoptosis, migration and invasion wer...
Source: Journal of Cellular and Molecular Medicine - January 29, 2018 Category: Molecular Biology Authors: Xiaoxia Wang, Wujian Xu, Ping Zhan, Tianxiang Xu, Jiajia Jin, Yingying Miu, Zejun Zhou, Qingqing Zhu, Bing Wan, Guangmin Xi, Liang Ye, Yafang Liu, Jianwei Gao, Huijuan Li, Tangfeng Lv, Yong Song Tags: Original Article Source Type: research

Leptin regulates disc cartilage endplate degeneration and ossification through activation of the MAPK ‐ERK signalling pathway in vivo and in vitro
Abstract Recent findings demonstrate that leptin plays a significant role in chondrocyte and osteoblast differentiation. However, the mechanisms by which leptin acts on cartilage endplate (CEP) cells to give rise to calcification are still unclear. The aim of this study was to evaluate the effects of leptin that induced mineralization of CEP cells in vitro and in vivo. We constructed a rat model of lumbar disc degeneration and determined that leptin was highly expressed in the presence of CEP calcification. Rat CEP cells treated with or without leptin were used for in vitro analysis using RT‐PCR and Western blotting t...
Source: Journal of Cellular and Molecular Medicine - January 26, 2018 Category: Molecular Biology Authors: Ying ‐Chao Han, Bin Ma, Song Guo, Mingjie Yang, Li‐Jun Li, Shan‐Jin Wang, Jun Tan Tags: Original Article Source Type: research

GP73 promotes invasion and metastasis of bladder cancer by regulating the epithelial –mesenchymal transition through the TGF‐β1/Smad2 signalling pathway
This study investigated the effects of Golgi membrane protein 73 (GP73) on the epithelial–mesenchymal transition (EMT) and on bladder cancer cell invasion and metastasis through the TGF‐β1/Smad2 signalling pathway. Paired bladder cancer and adjacent tissue samples (102) and normal bladder tissue samples (106) were obtained. Bladder cancer cell lines (T24, 5637, RT4, 253J and J82) were selected and assigned to blank, negative control (NC), TGF‐β, thrombospondin‐1 (TSP‐1), TGF‐β1+ TSP‐1, GP73‐siRNA‐1, GP73‐siRNA‐2, GP73‐siRNA‐1+ TSP‐1, GP73‐siRNA‐1+ pcDNA‐GP73, WT1‐siRNA and WT1‐siRNA...
Source: Journal of Cellular and Molecular Medicine - January 1, 2018 Category: Molecular Biology Authors: Han ‐Jie Yang, Ge‐Liang Liu, Bo Liu, Tian Liu Tags: Original Article Source Type: research

Endogenous hormone 2 ‐methoxyestradiol suppresses venous hypertension‐induced angiogenesis through up‐ and down‐regulating p53 and id‐1
This study was conducted to examine whether and how 2‐ME could improve the vascular malformations. Intracranial venous hypertension (VH) model produced in adult male Sprague‐Dawley rats and culture of human umbilical vein endothelial cells (HUVECs) at the anoxia condition were used to induce in vivo and in vitro angiogenesis, respectively. Lentiviral vectors of ID‐1 and p53 genes and of their siRNA were intracranially injected into rats and transfected into HUVECs to overexpress and down‐regulate these molecules. 2‐ME treatment not only reduced the in vivo progression of brain tissue angiogenesis in the intrac...
Source: Journal of Cellular and Molecular Medicine - November 29, 2017 Category: Molecular Biology Authors: Xiang Zou, Li Zhang, Jie Yuan, Chunjie Yang, Zehan Wu, Jianping Song, Wei Zhu, Ying Mao, Liang Chen Tags: Original Article Source Type: research

Down ‐regulating Myoferlin inhibits the vasculogenic mimicry of melanoma via decreasing MMP‐2 and inducing mesenchymal‐to‐epithelial transition
In conclusion, MYOF plays an important role in VM and knockdown of MYOF suppresses VM formation via decreasing MMP‐2 and inducing MET in A375 melanoma cells.
Source: Journal of Cellular and Molecular Medicine - November 22, 2017 Category: Molecular Biology Authors: Wenxue Zhang, Ping Zhou, Ai Meng, Rongxin Zhang, Yan Zhou Tags: Original Article Source Type: research

Complement 5a ‐mediated trophoblasts dysfunction is involved in the development of pre‐eclampsia
In this study, we reported that complement 5a (C5a) plays a pivotal role in aberrant placentation, which is essential for the onset of PE. We detected an elevated C5a deposition in macrophages and C5a receptor (C5aR) expression in trophoblasts of pre‐eclamptic placentas. Further study showed that C5a stimulated trophoblasts towards an anti‐angiogenic phenotype by mediating the imbalance of angiogenic factors such as soluble fms‐like tyrosine kinase 1 (sFlt1) and placental growth factor (PIGF). Additionally, C5a inhibited the migration and tube formation of trophoblasts, while, C5aR knockdown with siRNA rescued migrat...
Source: Journal of Cellular and Molecular Medicine - November 1, 2017 Category: Molecular Biology Authors: Yu Ma, Ling ‐Ran Kong, Qian Ge, Yuan‐Yuan Lu, Mo‐Na Hong, Yu Zhang, Cheng‐Chao Ruan, Ping‐Jin Gao Tags: Original Article Source Type: research

Autophagy promotes fibrosis and apoptosis in the peritoneum during long ‐term peritoneal dialysis
In this study, we found that the coincidence of elevated TGF‐β1 expression, autophagy, apoptosis and fibrosis in peritoneal membrane from patients with peritoneal dialysis. The peritoneal membranes from patients were performed with immunocytochemistry and transmission electron microscopy. Human peritoneal mesothelial cells were treated with 1.5%, 2.5% and 4.25% HGPDS for 24 hrs; Human peritoneal mesothelial cells pre‐treated with TGF‐β1 (10 ng/ml) or transfected with siRNA Beclin1 were treated with 4.25% HGPDS or vehicle for 24 hrs. We further detected the production of TGF‐β1, activation of TGF‐β1/Smad2/3 si...
Source: Journal of Cellular and Molecular Medicine - October 27, 2017 Category: Molecular Biology Authors: Jingjing Wu, Changying Xing, Li Zhang, Huijuan Mao, Xuguan Chen, Mingxing Liang, Fang Wang, Haibin Ren, Hongqing Cui, Aiqin Jiang, Zibin Wang, Meijuan Zou, Yong Ji Tags: Original Article Source Type: research

Aquaporin ‐1 inhibition reduces metastatic formation in a mouse model of melanoma
In conclusion, these findings highlight an additional role for AQP1 as an important determinant of tumour dissemination by facilitating tumour cell extravasation and metastatic formation. This study adds knowledge on the role played by AQP1 in tumour biology and supports the view of AQP1 as a potential drug target for cancer therapy.
Source: Journal of Cellular and Molecular Medicine - October 1, 2017 Category: Molecular Biology Authors: Laura Simone, Concetta Domenica Gargano, Francesco Pisani, Antonio Cibelli, Maria Grazia Mola, Antonio Frigeri, Maria Svelto, Grazia Paola Nicchia Tags: Original Article Source Type: research