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Crosstalk between hydrogen sulfide and nitric oxide in endothelial cells
Abstract Hydrogen sulfide (H2S) and nitric oxide (NO) are major gasotransmitters produced in endothelial cells (ECs), contributing to the regulation of vascular contractility and structural integrity. Their interaction at different levels would have a profound impact on angiogenesis. Here, we showed that H2S and NO stimulated the formation of new microvessels. Incubation of human umbilical vein endothelial cells (HUVECs‐926) with NaHS (a H2S donor) stimulated the phosphorylation of endothelial NO synthase (eNOS) and enhanced NO production. H2S had little effect on eNOS protein expression in ECs. L‐cysteine, a precursor...
Source: Journal of Cellular and Molecular Medicine - June 7, 2013 Category: Molecular Biology Authors: Zaid Altaany, Guangdong Yang, Rui Wang Tags: Original Article Source Type: research

Up‐regulation of stomatin expression by hypoxia and glucocorticoid stabilizes membrane‐associated actin in alveolar epithelial cells
In this study we found that hypoxia and dexamethasone (dex), a synthetic GC not only up‐regulated the expression of stomatin alone, but also imposed additive effect on the expression of stomatin in A549 cells, primary AEC and lung of rats. Then we investigated whether hypoxia and dex transcriptionally up‐regulated the expression of stomatin by reporter gene assay, and found that dex, but not hypoxia could increase the activity of a stomatin promoter‐driven reporter gene. Further deletion and mutational studies demonstrated that a GC response element (GRE) within the promoter region mainly contributed to the induction...
Source: Journal of Cellular and Molecular Medicine - May 15, 2013 Category: Molecular Biology Authors: Ji‐Cheng Chen, Hao‐Yu Cai, Yan Wang, Yuan‐Yuan Ma, Liang‐Nian Song, Li‐Juan Yin, Dong‐Mei Cao, Fei Diao, Yi‐Dong Li, Jian Lu Tags: Original Article Source Type: research

Evaluation of nasal epithelium sampling as a tool in the preclinical development of siRNA‐based therapeutics for asthma
Abstract The development of siRNA‐based asthma therapeutics is currently hampered by a paucity of relevant biomarkers and the need to ascertain tissue‐specific gene targeting in the context of active disease. Epithelial STAT6 expression is fundamental to asthma pathogenesis in which inflammatory changes are found throughout the respiratory tract. Therefore, to improve preclinical evaluation, we tested the efficacy of STAT6‐targeting siRNA within nasal epithelial cells (NEC's) obtained from asthmatic and non‐asthmatic donors. STAT6 expression was invariant in both donor groups and amenable to suppression by siRNA tr...
Source: Journal of Cellular and Molecular Medicine - February 13, 2013 Category: Molecular Biology Authors: Gareth D Healey, Neil Evans, Julian M Hopkin, Gwyneth Davies, William Walker Tags: Original Article Source Type: research

Acute hyperglycaemia enhances oxidative stress and aggravates myocardial ischaemia/reperfusion injury: role of thioredoxin‐interacting protein
This study aimed to investigate whether or not hyperglycaemia enhances Txnip expression in myocardial ischaemia/reperfusion (MI/R) and consequently exacerbates MI/R injury. Rats were subjected to 30 min. of left coronary artery ligation followed by 4 hrs of reperfusion and treated with saline or high glucose (HG, 500 g/l, 4 ml/kg/h intravenously). In vitro study was performed on cultured rat cardiomyocytes subjected to simulated ischaemia/reperfusion (SI/R) and incubated with HG (25 mM) or normal glucose (5.6 mM) medium. In vivo HG infusion during MI/R significantly impaired cardiac function, aggravated myocardial in...
Source: Journal of Cellular and Molecular Medicine - January 11, 2013 Category: Molecular Biology Authors: Hui Su, Lele Ji, Wenjuan Xing, Wei Zhang, Heping Zhou, Xinhong Qian, Xiaoming Wang, Feng Gao, Xin Sun, Haifeng Zhang Tags: Original Article Source Type: research

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