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Source: Journal of Cellular and Molecular Medicine
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Total 94 results found since Jan 2013.
CARD9 gene silencing with siRNA protects rats against severe acute pancreatitis: CARD9 ‐dependent NF‐κB and P38MAPKs pathway
In this study, small interfering RNA (siRNA) was used to reduce the levels of CARD9 expression in sodium taurocholate‐stimulated SAP rats. CARD9 was overexpressed in SAP rats, which correlated with the severity of pancreatitis. When compared to the untreated group, the cohort that received the siRNA treatment demonstrated a significant reduction in pancreatic injury, neutrophil infiltration, myeloperoxidase activity and pro‐inflammatory cytokines. Furthermore, siRNAs showed that the reduction of CARD9 in SAP rats down‐regulated the expression of NF‐κBp65 and P38MAPK which are involved in the transcription and rele...
Source: Journal of Cellular and Molecular Medicine - November 30, 2016 Category: Molecular Biology Authors: Zhi ‐wen Yang, Xiao‐xiao Meng, Chun Zhang, Ping Xu Tags: Original Article Source Type: research
Evaluation of nasal epithelium sampling as a tool in the preclinical development of siRNA‐based therapeutics for asthma
Abstract
The development of siRNA‐based asthma therapeutics is currently hampered by a paucity of relevant biomarkers and the need to ascertain tissue‐specific gene targeting in the context of active disease. Epithelial STAT6 expression is fundamental to asthma pathogenesis in which inflammatory changes are found throughout the respiratory tract. Therefore, to improve preclinical evaluation, we tested the efficacy of STAT6‐targeting siRNA within nasal epithelial cells (NEC's) obtained from asthmatic and non‐asthmatic donors. STAT6 expression was invariant in both donor groups and amenable to suppression by siRNA tr...
Source: Journal of Cellular and Molecular Medicine - February 13, 2013 Category: Molecular Biology Authors: Gareth D Healey, Neil Evans, Julian M Hopkin, Gwyneth Davies, William Walker Tags: Original Article Source Type: research
Placental growth factor silencing ameliorates liver fibrosis and angiogenesis and inhibits activation of hepatic stellate cells in a murine model of chronic liver disease
This study aimed to assess the effect of reducing PlGF expression using small interfering RNA (siRNA) on experimental liver fibrosis and angiogenesis, and to elucidate the underlying molecular mechanisms. Fibrosis was induced in mice by carbon tetrachloride (CCl4) for 8 weeks, and mice were treated with PlGF siRNA or non‐targeting control siRNA starting two weeks after initiating CCl4 injections. The results showed that PlGF was highly expressed in cirrhotic human and mice livers; which mainly distributed in activated hepatic stellate cells (HSCs). PlGF silencing robustly reduced liver inflammation, fibrosis, intrahepati...
Source: Journal of Cellular and Molecular Medicine - April 1, 2017 Category: Molecular Biology Authors: Xi Li, Qun ‐Yan Yao, Hong‐Chun Liu, Qian‐Wen Jin, Bei‐Li Xu, Shun‐Cai Zhang, Chuan‐Tao Tu Tags: Original Article Source Type: research
GP73 promotes invasion and metastasis of bladder cancer by regulating the epithelial –mesenchymal transition through the TGF‐β1/Smad2 signalling pathway
This study investigated the effects of Golgi membrane protein 73 (GP73) on the epithelial–mesenchymal transition (EMT) and on bladder cancer cell invasion and metastasis through the TGF‐β1/Smad2 signalling pathway. Paired bladder cancer and adjacent tissue samples (102) and normal bladder tissue samples (106) were obtained. Bladder cancer cell lines (T24, 5637, RT4, 253J and J82) were selected and assigned to blank, negative control (NC), TGF‐β, thrombospondin‐1 (TSP‐1), TGF‐β1+ TSP‐1, GP73‐siRNA‐1, GP73‐siRNA‐2, GP73‐siRNA‐1+ TSP‐1, GP73‐siRNA‐1+ pcDNA‐GP73, WT1‐siRNA and WT1‐siRNA...
Source: Journal of Cellular and Molecular Medicine - January 1, 2018 Category: Molecular Biology Authors: Han ‐Jie Yang, Ge‐Liang Liu, Bo Liu, Tian Liu Tags: Original Article Source Type: research
Effect of CLIC1 gene silencing on proliferation, migration, invasion and apoptosis of human gallbladder cancer cells
This study aimed to explore the effects of CLIC1 gene silencing on proliferation, migration, invasion and apoptosis of human gallbladder cancer (GBC). GBC and normal gallbladder tissues were extracted for the detection of mRNA and protein expressions of CLIC1. GBC‐SD and NOZ cells in the logarithmic growth phase were selected to conduct the experiment. Three different siRNA recombined expression vectors were established using CLIC1 as a target at different sites. Reverse transcription quantitative polymerase chain reaction (RT‐qPCR) and Western blotting were, respectively, used to detect the CLIC1 mRNA and protein expr...
Source: Journal of Cellular and Molecular Medicine - March 8, 2018 Category: Molecular Biology Authors: Yue ‐Ming He, Zhong‐Lin Zhang, Quan‐Yan Liu, Yu‐Sha Xiao, Lei Wei, Chen Xi, Xiang Nan Tags: Original Article Source Type: research
Zinc rescues obesity ‐induced cardiac hypertrophy via stimulating metallothionein to suppress oxidative stress‐activated BCL10/CARD9/p38 MAPK pathway
In this study, we investigated the mechanisms by which zinc inactivates p38 MAPK to prevent ORCH.
Mice (4‐week old) were fed either high fat diet (HFD, 60% kcal fat) or normal diet (ND, 10% kcal fat) containing variable amounts of zinc (deficiency, normal and supplement) for 3 and 6 months. P38 MAPK siRNA and the p38 MAPK inhibitor SB203580 were used to suppress p38 MAPK activity in vitro and in vivo, respectively. HFD activated p38 MAPK and increased expression of B‐cell lymphoma/CLL 10 (BCL10) and caspase recruitment domain family member 9 (CARD9). These responses were enhanced by zinc deficiency and attenuated by zi...
Source: Journal of Cellular and Molecular Medicine - February 2, 2017 Category: Molecular Biology Authors: Shudong Wang, Junlian Gu, Zheng Xu, Zhiguo Zhang, Tao Bai, Jianxiang Xu, Jun Cai, Gregory Barnes, Qiu ‐Ju Liu, Jonathan H. Freedman, Yonggang Wang, Quan Liu, Yang Zheng, Lu Cai Tags: Original Article Source Type: research
Overexpressing STAMP2 attenuates adipose tissue angiogenesis and insulin resistance in diabetic ApoE −/−/LDLR−/− mouse via a PPARγ/CD36 pathway
Abstract
The aim of this study was to investigate whether overexpression of STAMP2 improves insulin resistance by regulating angiogenesis in adipose tissues. The characteristics of diabetic mice were measured by serial metabolite and pathology tests. Samples were obtained from epididymal, subcutaneous and brown adipose tissues. Histological and morphological analysis demonstrated that STAMP2 gene overexpression reduced adipocyte size, angiogenesis in epididymal and brown adipose tissues. On aortic ring assay, microvessels sprouting from aortas were significantly inhibited after STAMP2 gene overexpression. The cellular effe...
Source: Journal of Cellular and Molecular Medicine - June 19, 2017 Category: Molecular Biology Authors: Feng Wang, Lu Han, Ran ‐ran Qin, Yao‐yuan Zhang, Di Wang, Zhi‐Hao Wang, Meng‐Xiong Tang, Yun Zhang, Ming Zhong, Wei Zhang Tags: Original Article Source Type: research
Autophagy promotes fibrosis and apoptosis in the peritoneum during long ‐term peritoneal dialysis
In this study, we found that the coincidence of elevated TGF‐β1 expression, autophagy, apoptosis and fibrosis in peritoneal membrane from patients with peritoneal dialysis. The peritoneal membranes from patients were performed with immunocytochemistry and transmission electron microscopy. Human peritoneal mesothelial cells were treated with 1.5%, 2.5% and 4.25% HGPDS for 24 hrs; Human peritoneal mesothelial cells pre‐treated with TGF‐β1 (10 ng/ml) or transfected with siRNA Beclin1 were treated with 4.25% HGPDS or vehicle for 24 hrs. We further detected the production of TGF‐β1, activation of TGF‐β1/Smad2/3 si...
Source: Journal of Cellular and Molecular Medicine - October 27, 2017 Category: Molecular Biology Authors: Jingjing Wu, Changying Xing, Li Zhang, Huijuan Mao, Xuguan Chen, Mingxing Liang, Fang Wang, Haibin Ren, Hongqing Cui, Aiqin Jiang, Zibin Wang, Meijuan Zou, Yong Ji Tags: Original Article Source Type: research
Overexpression of STAMP2 suppresses atherosclerosis and stabilizes plaques in diabetic mice
Abstract
Our research aims to evaluate the function of the STAMP2 gene, an important trigger in insulin resistance (IR), and explore its role in macrophage apoptosis in diabetic atherosclerotic vulnerable plaques. The characteristics of diabetic mice were measured by serial metabolite and pathology tests. The level of STAMP2 was measured by RT‐PCR and Western blot. The plaque area, lipid and collagen content of brachiocephalic artery plaques were measured by histopathological analyses, and the macrophage apoptosis was measured by TUNEL. Correlation of STAMP2/Akt signaling pathway and macrophage apoptosis was validated by...
Source: Journal of Cellular and Molecular Medicine - January 22, 2014 Category: Molecular Biology Authors: Jia Wang, Lu Han, Zhi‐hao Wang, Wen‐yuan Ding, Yuan‐yuan Shang, Meng‐xiong Tang, Wen‐bo Li, Yun Zhang, Wei Zhang, Ming Zhong Tags: Original Article Source Type: research
MiRNA‐30a inhibits AECs‐II apoptosis by blocking mitochondrial fission dependent on Drp‐1
This study demonstrated that miR‐30a inhibited AECs‐II apoptosis by repressing the mitochondrial fission dependent on Drp‐1, and could function as a novel therapeutic target for lung fibrosis.
Source: Journal of Cellular and Molecular Medicine - October 6, 2014 Category: Molecular Biology Authors: Cuiping Mao, Jinjin Zhang, Shengcui Lin, Lili Jing, Jing Xiang, Meirong Wang, Bingsi Wang, Pan Xu, Weili Liu, Xiaodong Song, Changjun Lv Tags: Original Article Source Type: research
BMP‐9 regulates the osteoblastic differentiation and calcification of vascular smooth muscle cells through an ALK1 mediated pathway
Abstract
The process of vascular calcification shares many similarities with that of physiological skeletal mineralization, and involves the deposition of hydroxyapatite crystals in arteries. However, the cellular mechanisms responsible have yet to be fully explained. Bone morphogenetic protein (BMP‐9) has been shown to exert direct effects on both bone development and vascular function. In the present study, we have investigated the role of BMP‐9 in vascular smooth muscle cell (VSMC) calcification. Vessel calcification in chronic kidney disease (CKD) begins pre‐dialysis, with factors specific to the dialysis milieu ...
Source: Journal of Cellular and Molecular Medicine - October 9, 2014 Category: Molecular Biology Authors: Dongxing Zhu, Neil Charles Wallace Mackenzie, Catherine M. Shanahan, Rukshana C. Shroff, Colin Farquharson, Vicky Elisabeth MacRae Tags: Original Article Source Type: research
HSPA12B inhibits lipopolysaccharide‐induced inflammatory response in human umbilical vein endothelial cells
This study investigated the effects of HSPA12B on lipopolysaccharide (LPS)‐induced inflammatory responses in human umbilical vein endothelial cells (HUVECs) and the possible mechanisms involved. A HUVECs inflammatory model was induced by LPS. Overexpression of HSPA12B in HUVECs was achieved by infection with recombinant adenoviruses encoding green fluorescence protein‐HSPA12B. Knockdown of HSPA12B was achieved by siRNA technique. Twenty four hours after virus infection or siRNA transfection, HUVECs were stimulated with 1 μg/ml LPS for 4 hrs. Endothelial cell permeability ability was determined by transwell permeabil...
Source: Journal of Cellular and Molecular Medicine - December 1, 2014 Category: Molecular Biology Authors: Jun Wu, Xuehan Li, Lei Huang, Surong Jiang, Fei Tu, Xiaojin Zhang, He Ma, Rongrong Li, Chuanfu Li, Yuehua Li, Zhengnian Ding, Li Liu Tags: Original Article Source Type: research
Qiliqiangxin inhibits angiotensin II‐induced transdifferentiation of rat cardiac fibroblasts through suppressing interleukin‐6
This study aimed to investigate the effects of QL on angiotensin II (AngII)‐induced CFs transdifferentiation. Study was performed on in vitro cultured CFs from Sprague–Dawley rats. CFs differentiation was induced by AngII, which was attenuated by QL through reducing transforming growth factor‐β1 (TGF‐β1) and α‐smooth muscle actin (α‐SMA). Our data showed that AngII‐induced IL‐6 mRNA as well as typeI and typeIII collagens were reduced by QL. IL‐6 deficiency could suppress TGF‐β1 and α‐SMA, and both IL‐6 siRNA and QL‐mediated such effect was reversed by foresed expression of recombined IL‐6....
Source: Journal of Cellular and Molecular Medicine - March 6, 2015 Category: Molecular Biology Authors: Jingmin Zhou, Kun Jiang, Xuefeng Ding, Mingqiang Fu, Shijun Wang, Lingti Zhu, Tao He, Jingfeng Wang, Aijun Sun, Kai Hu, Li Chen, Yunzeng Zou, Junbo Ge Tags: Original Article Source Type: research
A novel TXNIP‐based mechanism for Cx43‐mediated regulation of oxidative drug injury
Abstract
Gap junctions (GJs) play an important role in the regulation of cell response to many drugs. However, little is known about their mechanisms. Using an in vitro model of cytotoxicity induced by geneticin (G418), we explored the potential signalling mechanisms involved. Incubation of cells with G418 resulted in cell death, as indicated by the change in cell morphology, loss of cell viability and activation of caspase‐3. Before the onset of cell injury, G418 induced reactive oxygen species (ROS) generation, activated oxidative sensitive kinase P38 and caused a shift of connexin 43 (Cx43) from non‐phosphorylated ...
Source: Journal of Cellular and Molecular Medicine - July 8, 2015 Category: Molecular Biology Authors: Kun Gao, Yuan Chi, Xiling Zhang, Hui Zhang, Gang Li, Wei Sun, Masayuki Takeda, Jian Yao Tags: Original Article Source Type: research
HIF‐1α regulates EMT via the Snail and β‐catenin pathways in paraquat poisoning‐induced early pulmonary fibrosis
Abstract
Paraquat (PQ) poisoning‐induced pulmonary fibrosis is one of the primary causes of death in patients with PQ poisoning. Hypoxia‐inducible factor‐1α (HIF‐1α) and epithelial‐mesenchymal transition (EMT) are involved in the progression of pulmonary fibrosis. Snail and β‐catenin are two other factors involved in promoting EMT. However, the relationship among HIF‐1α, Snail and β‐catenin in PQ poisoning‐induced pulmonary fibrosis is not clear. Our research aimed to determine whether the regulation of HIF‐1α in EMT occurs via the Snail and β‐catenin pathways in PQ poisoning‐induced pulmonar...
Source: Journal of Cellular and Molecular Medicine - January 19, 2016 Category: Molecular Biology Authors: Yong Zhu, Jiuting Tan, Hui Xie, Jinfeng Wang, Xiaoxiao Meng, Ruilan Wang Tags: Original Article Source Type: research
