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Source: Journal of Cellular and Molecular Medicine

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Total 94 results found since Jan 2013.

Changes in short‐chain acyl‐coA dehydrogenase during rat cardiac development and stress
This study was designed to investigate the expression of short‐chain acyl‐CoA dehydrogenase (SCAD), a key enzyme of fatty acid β‐oxidation, during rat heart development and the difference of SCAD between pathological and physiological cardiac hypertrophy. The expression of SCAD was lowest in the foetal and neonatal heart, which had time‐dependent increase during normal heart development. In contrast, a significant decrease in SCAD expression was observed in different ages of spontaneously hypertensive rats (SHR). On the other hand, swim‐trained rats developed physiological cardiac hypertrophy, whereas SHR develo...
Source: Journal of Cellular and Molecular Medicine - March 8, 2015 Category: Molecular Biology Authors: Jinxian Huang, Lipeng Xu, Qiuju Huang, Jiani Luo, Peiqing Liu, Shaorui Chen, Xi Yuan, Yao Lu, Ping Wang, Sigui Zhou Tags: Original Article Source Type: research

Long non‐coding RNA MALAT1 regulates hyperglycaemia induced inflammatory process in the endothelial cells
Abstract To examine whether the long non‐coding RNA (lncRNA) metastasis associated lung adenocarcinoma transcript 1 (MALAT1) is altered in the endothelial cells in response to glucose and the significance of such alteration. We incubated human umbilical vein endothelial cells with media containing various glucose levels. We found an increase in MALAT1 expression peaking after 12 hrs of incubation in high glucose. This increase was associated with parallel increase in serum amyloid antigen 3 (SAA3), an inflammatory ligand and target of MALAT1 and was further accompanied by increase in mRNAs and proteins of inflammatory m...
Source: Journal of Cellular and Molecular Medicine - March 19, 2015 Category: Molecular Biology Authors: Prasanth Puthanveetil, Shali Chen, Biao Feng, Anirudh Gautam, Subrata Chakrabarti Tags: Original Article Source Type: research

Identification of C‐terminal Hsp70‐interacting protein as a mediator of tumour necrosis factor action in osteoblast differentiation by targeting osterix for degradation
Abstract In patients with inflammatory arthritis, tumour necrosis factor (TNF)‐α are overproduced in inflamed joints. This leads to local erosion of cartilage and bone, periarticular osteopenia, as well as osteoporosis. But less is known regarding the molecular mechanisms that mediate the effect of TNF‐α on osteoblast function. The purpose of this study was to test that C terminus of Hsc70‐interacting protein (CHIP) has a specific role in suppressing the osteogenic activity of osteoblasts under inflammatory conditions. C2C12, MC3T3‐E1 and HEK293T cell lines were cultured and cotransfected with related plasmids. A...
Source: Journal of Cellular and Molecular Medicine - March 26, 2015 Category: Molecular Biology Authors: Jianmin Xie, Jieruo Gu Tags: Original Article Source Type: research

Histone deacetylase 5 regulates the inflammatory response of macrophages
Abstract Modifying the chromatin structure and interacting with non‐histone proteins, histone deacetylases (HDAC) are involved in vital cellular processes at different levels. We here specifically investigated the direct effects of HDAC5 in macrophage activation in response to bacterial or cytokine stimuli. Using murine and human macrophage cell lines, we studied the expression profile and the immunological function of HDAC5 at transcription and protein level in over‐expression as well as RNA interference experiments. Toll‐like receptor‐mediated stimulation of murine RAW264.7 cells significantly reduced HDAC5 mRNA ...
Source: Journal of Cellular and Molecular Medicine - June 8, 2015 Category: Molecular Biology Authors: Lukas Poralla, Thorsten Stroh, Ulrike Erben, Marie Sittig, Sven Liebig, Britta Siegmund, Rainer Glauben Tags: Original Article Source Type: research

Cul4A overexpression associated with Gli1 expression in malignant pleural mesothelioma
In this study, we analysed clinical mesothelioma tumours and found moderate to strong expression of Cul4A in 70.9% (51/72) of these tumours, as shown by immunohistochemistry. In 72.2% mesothelioma tumours with increased Cul4A copy number identified by fluorescence in situ hybridization analysis, Cul4A protein expression was moderate to strong. Similarly, Cul4A was overexpressed and Cul4A copy number was increased in human mesothelioma cell lines. Because Gli1 is highly expressed in human mesothelioma cells, we compared Cul4A and Gli1 expression in mesothelioma tumours and found their expression associated (P < 0.05, ...
Source: Journal of Cellular and Molecular Medicine - July 27, 2015 Category: Molecular Biology Authors: Yi‐Lin Yang, Jian Ni, Ping‐Chih Hsu, Jian‐Hua Mao, David Hsieh, Angela Xu, Geraldine Chan, Alfred Au, Zhidong Xu, David M. Jablons, Liang You Tags: Original Article Source Type: research

Epidermal growth factor attenuates blood‐spinal cord barrier disruption via PI3K/Akt/Rac1 pathway after acute spinal cord injury
In this study, we demonstrate that EGF administration inhibits the disruption of BSCB permeability and improves the locomotor activity in SCI model rats. Inhibition of the PI3K/Akt pathways by a specific inhibitor, LY294002, suppresses EGF‐induced Rac1 activation as well as tight junction (TJ) and adherens junction (AJ) expression. Furthermore, the protective effect of EGF on BSCB is related to the activation of Rac1 both in vivo and in vitro. Blockade of Rac1 activation with Rac1 siRNA downregulates EGF‐induced TJ and AJ proteins expression in endothelial cells. Taken together, our results indicate that EGF treatment ...
Source: Journal of Cellular and Molecular Medicine - January 1, 2016 Category: Molecular Biology Authors: Binbin Zheng, Libing Ye, Yulong Zhou, Sipin Zhu, Qingqing Wang, Hongxue Shi, Daqing Chen, Xiaojie Wei, Zhouguang Wang, Xiaokun Li, Jian Xiao, Huazi Xu, Hongyu Zhang Tags: Original Article Source Type: research

The mechanisms and significance of up‐regulation of RhoB expression by hypoxia and glucocorticoid in rat lung and A549 cells
In this study, we investigated the effects of hypoxia or/and GC on the expression and activition of RhoB in the lung of rats and human A549 lung carcinoma cells, and further studied its mechanism and significance. We found that hypoxia and dexamethasone (Dex), a synethic GC, not only significantly increased the expression and activation of RhoB independently but also coregulated the expresion of RhoB in vitro and in vivo. Up‐regulation of RhoB by hypoxia was in part through stabilizing the RhoB mRNA and protein. Inhibiting hypoxia‐activated hypoxia‐inducible transcription factor‐1α (HIF‐1α), c‐Jun N‐termina...
Source: Journal of Cellular and Molecular Medicine - February 24, 2016 Category: Molecular Biology Authors: Gao‐Xiang Huang, Xiao‐Yu Pan, Yi‐Duo Jin, Yan Wang, Xiao‐Lian Song, Chang‐Hui Wang, Yi‐Dong Li, Jian Lu Tags: Original Article Source Type: research

Aspirin induces Nrf2‐mediated transcriptional activation of haem oxygenase‐1 in protection of human melanocytes from H2O2‐induced oxidative stress
Abstract The removal of hydrogen peroxide (H2O2) by antioxidants has been proven to be beneficial to patients with vitiligo. Aspirin (acetylsalicylic acid, ASA) has antioxidant activity and has great preventive and therapeutical effect in many oxidative stress‐relevant diseases. Whether ASA can protect human melanocytes against oxidative stress needs to be further studied. Here, we investigated the potential protective effect and mechanisms of ASA against H2O2‐induced oxidative injury in human melanocytes. Human melanocytes were pre‐treated with different concentrations of ASA, followed by exposure to 1.0 mM H2O2. Ce...
Source: Journal of Cellular and Molecular Medicine - March 10, 2016 Category: Molecular Biology Authors: Zhe Jian, Lingzhen Tang, Xiuli Yi, Bangmin Liu, Qian Zhang, Guannan Zhu, Gang Wang, Tianwen Gao, Chunying Li Tags: Original Article Source Type: research

Effects of short‐chain acyl‐CoA dehydrogenase on cardiomyocyte apoptosis
In conclusion, for the first time our findings directly demonstrated that SCAD may be as a new target to prevent cardiomyocyte apoptosis through the AMPK/PPARα/SCAD signal pathways.
Source: Journal of Cellular and Molecular Medicine - March 17, 2016 Category: Molecular Biology Authors: Zhenhua Zeng, Qiuju Huang, Zhaohui Shu, Peiqing Liu, Shaorui Chen, Xuediao Pan, Linquan Zang, Sigui Zhou Tags: Original Article Source Type: research

MicroRNA‐20a‐5p contributes to hepatic glycogen synthesis through targeting p63 to regulate p53 and PTEN expression
In conclusion, we found novel evidence suggesting that as a member of miR‐17 family, miR‐20a‐5p contributes to hepatic glycogen synthesis through targeting p63 to regulate p53 and PTEN expression.
Source: Journal of Cellular and Molecular Medicine - March 27, 2016 Category: Molecular Biology Authors: Weiwei Fang, Jun Guo, Yuan Cao, Shuyue Wang, Cheng Pang, Meng Li, Lin Dou, Yong Man, Xiuqing Huang, Tao Shen, Jian Li Tags: Original Article Source Type: research

Hypoxanthine induces cholesterol accumulation and incites atherosclerosis in apolipoprotein E‐deficient mice and cells
In this study, we investigated the role of hypoxanthine on cholesterol synthesis and atherosclerosis development, particularly in apolipoprotein E (APOE)‐deficient mice. The effect of hypoxanthine on the regulation of cholesterol synthesis and atherosclerosis were evaluated in Apoe knockout (KO) mice and cultured HepG2 cells. Hypoxanthine markedly increased serum cholesterol levels and the atherosclerotic plaque area in Apoe KO mice. In HepG2 cells, hypoxanthine increased intracellular ROS production. Hypoxanthine increased cholesterol accumulation and decreased APOE and ATP‐binding cassette transporter A1 (ABCA1) mRNA...
Source: Journal of Cellular and Molecular Medicine - July 10, 2016 Category: Molecular Biology Authors: Hye‐Myung Ryu, You‐Jin Kim, Eun‐Joo Oh, Se‐Hyun Oh, Ji‐Young Choi, Jang‐Hee Cho, Chan‐Duck Kim, Sun‐Hee Park, Yong‐Lim Kim Tags: Original Article Source Type: research

Hypoxanthine induces cholesterol accumulation and incites atherosclerosis in apolipoprotein E ‐deficient mice and cells
In this study, we investigated the role of hypoxanthine on cholesterol synthesis and atherosclerosis development, particularly in apolipoprotein E (APOE)‐deficient mice. The effect of hypoxanthine on the regulation of cholesterol synthesis and atherosclerosis were evaluated in Apoe knockout (KO) mice and cultured HepG2 cells. Hypoxanthine markedly increased serum cholesterol levels and the atherosclerotic plaque area in Apoe KO mice. In HepG2 cells, hypoxanthine increased intracellular ROS production. Hypoxanthine increased cholesterol accumulation and decreased APOE and ATP‐binding cassette transporter A1 (ABCA1) mRNA...
Source: Journal of Cellular and Molecular Medicine - July 10, 2016 Category: Molecular Biology Authors: Hye ‐Myung Ryu, You‐Jin Kim, Eun‐Joo Oh, Se‐Hyun Oh, Ji‐Young Choi, Jang‐Hee Cho, Chan‐Duck Kim, Sun‐Hee Park, Yong‐Lim Kim Tags: Original Article Source Type: research

The mechanisms and significance of up ‐regulation of RhoB expression by hypoxia and glucocorticoid in rat lung and A549 cells
In this study, we investigated the effects of hypoxia or/and GC on the expression and activition of RhoB in the lung of rats and human A549 lung carcinoma cells, and further studied its mechanism and significance. We found that hypoxia and dexamethasone (Dex), a synethic GC, not only significantly increased the expression and activation of RhoB independently but also coregulated the expresion of RhoB in vitro and in vivo. Up‐regulation of RhoB by hypoxia was in part through stabilizing the RhoB mRNA and protein. Inhibiting hypoxia‐activated hypoxia‐inducible transcription factor‐1α (HIF‐1α), c‐Jun N‐termina...
Source: Journal of Cellular and Molecular Medicine - February 23, 2016 Category: Molecular Biology Authors: Gao ‐Xiang Huang, Xiao‐Yu Pan, Yi‐Duo Jin, Yan Wang, Xiao‐Lian Song, Chang‐Hui Wang, Yi‐Dong Li, Jian Lu Tags: Original Article Source Type: research

Aspirin induces Nrf2 ‐mediated transcriptional activation of haem oxygenase‐1 in protection of human melanocytes from H2O2‐induced oxidative stress
Abstract The removal of hydrogen peroxide (H2O2) by antioxidants has been proven to be beneficial to patients with vitiligo. Aspirin (acetylsalicylic acid, ASA) has antioxidant activity and has great preventive and therapeutical effect in many oxidative stress‐relevant diseases. Whether ASA can protect human melanocytes against oxidative stress needs to be further studied. Here, we investigated the potential protective effect and mechanisms of ASA against H2O2‐induced oxidative injury in human melanocytes. Human melanocytes were pre‐treated with different concentrations of ASA, followed by exposure to 1.0 mM H2O2. Ce...
Source: Journal of Cellular and Molecular Medicine - March 9, 2016 Category: Molecular Biology Authors: Zhe Jian, Lingzhen Tang, Xiuli Yi, Bangmin Liu, Qian Zhang, Guannan Zhu, Gang Wang, Tianwen Gao, Chunying Li Tags: Original Article Source Type: research

Effects of short ‐chain acyl‐CoA dehydrogenase on cardiomyocyte apoptosis
In conclusion, for the first time our findings directly demonstrated that SCAD may be as a new target to prevent cardiomyocyte apoptosis through the AMPK/PPARα/SCAD signal pathways.
Source: Journal of Cellular and Molecular Medicine - March 16, 2016 Category: Molecular Biology Authors: Zhenhua Zeng, Qiuju Huang, Zhaohui Shu, Peiqing Liu, Shaorui Chen, Xuediao Pan, Linquan Zang, Sigui Zhou Tags: Original Article Source Type: research