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Source: Journal of Cellular and Molecular Medicine

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Total 94 results found since Jan 2013.

Mitofusin‐2 knockdown increases ER–mitochondria contact and decreases amyloid β‐peptide production
Abstract Mitochondria are physically and biochemically in contact with other organelles including the endoplasmic reticulum (ER). Such contacts are formed between mitochondria‐associated ER membranes (MAM), specialized subregions of ER, and the outer mitochondrial membrane (OMM). We have previously shown increased expression of MAM‐associated proteins and enhanced ER to mitochondria Ca2+ transfer from ER to mitochondria in Alzheimer's disease (AD) and amyloid β‐peptide (Aβ)‐related neuronal models. Here, we report that siRNA knockdown of mitofusin‐2 (Mfn2), a protein that is involved in the tethering of ER and ...
Source: Journal of Cellular and Molecular Medicine - April 30, 2016 Category: Molecular Biology Authors: Nuno Santos Leal, Bernadette Schreiner, Catarina Moreira Pinho, Riccardo Filadi, Birgitta Wiehager, Helena Karlström, Paola Pizzo, Maria Ankarcrona Tags: Original Article Source Type: research

Suppressive effect of epigallocatechin‐3‐O‐gallate on endoglin molecular regulation in myocardial fibrosis in vitro and in vivo
In conclusion, epigallocatechin‐3‐O‐gallate (EGCG) attenuated the endoglin expression and myocardial fibrosis by anti‐inflammatory effect in vitro and in vivo, the novel suppressive effect was mediated through JNK/AP‐1 pathway.
Source: Journal of Cellular and Molecular Medicine - June 15, 2016 Category: Molecular Biology Authors: Chiu‐Mei Lin, Hang Chang, Bao‐Wei Wang, Kou‐Gi Shyu Tags: Original Article Source Type: research

Suppressive effect of epigallocatechin ‐3‐O‐gallate on endoglin molecular regulation in myocardial fibrosis in vitro and in vivo
In conclusion, epigallocatechin‐3‐O‐gallate (EGCG) attenuated the endoglin expression and myocardial fibrosis by anti‐inflammatory effect in vitro and in vivo, the novel suppressive effect was mediated through JNK/AP‐1 pathway.
Source: Journal of Cellular and Molecular Medicine - June 15, 2016 Category: Molecular Biology Authors: Chiu ‐Mei Lin, Hang Chang, Bao‐Wei Wang, Kou‐Gi Shyu Tags: Original Article Source Type: research

Mitofusin ‐2 knockdown increases ER–mitochondria contact and decreases amyloid β‐peptide production
Abstract Mitochondria are physically and biochemically in contact with other organelles including the endoplasmic reticulum (ER). Such contacts are formed between mitochondria‐associated ER membranes (MAM), specialized subregions of ER, and the outer mitochondrial membrane (OMM). We have previously shown increased expression of MAM‐associated proteins and enhanced ER to mitochondria Ca2+ transfer from ER to mitochondria in Alzheimer's disease (AD) and amyloid β‐peptide (Aβ)‐related neuronal models. Here, we report that siRNA knockdown of mitofusin‐2 (Mfn2), a protein that is involved in the tethering of ER and ...
Source: Journal of Cellular and Molecular Medicine - May 19, 2016 Category: Molecular Biology Authors: Nuno Santos Leal, Bernadette Schreiner, Catarina Moreira Pinho, Riccardo Filadi, Birgitta Wiehager, Helena Karlstr öm, Paola Pizzo, Maria Ankarcrona Tags: Original Article Source Type: research

Silencing of CA1 mRNA in tumour cells does not change the gene expression of the extracellular matrix proteins
We report the silencing of CA1 mRNA in PC3 and MDA cells. The levels of mRNA coding CA1 protein in the knock‐down mRNA (CA1 siRNA) cells have been measured by RT‐PCR and were approximately 5% (PC3) and 20% (MDA‐MB‐231), respectively, of the level of control (Mock siRNA) used during silencing. In PC3 and MDA‐MB‐231 cells, the mRNAs for COL1A1 and COL4A4 were up‐regulated. The mRNAs for CTHRC1, LAMC2, and WNT7B were not changed when compared to the control. The morphology of the cells during the treatments remained the same. On the Western blots, the lysate from the silenced cells showed lower levels of CA I as well.
Source: Journal of Cellular and Molecular Medicine - August 7, 2017 Category: Molecular Biology Authors: Radivojka Vulic, Silvia Tyciakova, Maria Dubrovcakova, Ludovit Skultety, Jan Lakota Tags: Short Communication Source Type: research

Aquaporin ‐1 inhibition reduces metastatic formation in a mouse model of melanoma
In conclusion, these findings highlight an additional role for AQP1 as an important determinant of tumour dissemination by facilitating tumour cell extravasation and metastatic formation. This study adds knowledge on the role played by AQP1 in tumour biology and supports the view of AQP1 as a potential drug target for cancer therapy.
Source: Journal of Cellular and Molecular Medicine - October 1, 2017 Category: Molecular Biology Authors: Laura Simone, Concetta Domenica Gargano, Francesco Pisani, Antonio Cibelli, Maria Grazia Mola, Antonio Frigeri, Maria Svelto, Grazia Paola Nicchia Tags: Original Article Source Type: research

SIRT1/FoxO3 axis alteration leads to aberrant immune responses in bronchial epithelial cells
In conclusion, CSE impairs the function of SIRT1/FoxO3 axis in bronchial epithelium, dysregulating NF‐κB activity and inducing pro‐inflammatory responses.
Source: Journal of Cellular and Molecular Medicine - February 7, 2018 Category: Molecular Biology Authors: Serena Di Vincenzo, Irene H. Heijink, Jacobien A. Noordhoek, Chiara Cipollina, Liboria Siena, Andreina Bruno, Maria Ferraro, Dirkje S. Postma, Mark Gjomarkaj, Elisabetta Pace Tags: Original Article Source Type: research

Otx2 enhances transdifferentiation of M üller cells‐derived retinal stem cells into photoreceptor‐like cells
In conclusion, Otx2 enhances transdifferentiation of MC‐RSCs into photoreceptor‐like cells and this is associated with the inhibition of Wnt signalling. Otx2 is a potential target f or gene therapy of retinal degenerative diseases.
Source: Journal of Cellular and Molecular Medicine - November 19, 2018 Category: Molecular Biology Authors: Yu Xiong, Hongpei Ji, Zhipeng You, Fei Yao, Rongrong Zhou, Weitao Song, Xiaobo Xia Tags: ORIGINAL ARTICLE Source Type: research

Acute hyperglycaemia enhances oxidative stress and aggravates myocardial ischaemia/reperfusion injury: role of thioredoxin‐interacting protein
This study aimed to investigate whether or not hyperglycaemia enhances Txnip expression in myocardial ischaemia/reperfusion (MI/R) and consequently exacerbates MI/R injury. Rats were subjected to 30 min. of left coronary artery ligation followed by 4 hrs of reperfusion and treated with saline or high glucose (HG, 500 g/l, 4 ml/kg/h intravenously). In vitro study was performed on cultured rat cardiomyocytes subjected to simulated ischaemia/reperfusion (SI/R) and incubated with HG (25 mM) or normal glucose (5.6 mM) medium. In vivo HG infusion during MI/R significantly impaired cardiac function, aggravated myocardial in...
Source: Journal of Cellular and Molecular Medicine - January 11, 2013 Category: Molecular Biology Authors: Hui Su, Lele Ji, Wenjuan Xing, Wei Zhang, Heping Zhou, Xinhong Qian, Xiaoming Wang, Feng Gao, Xin Sun, Haifeng Zhang Tags: Original Article Source Type: research

Up‐regulation of stomatin expression by hypoxia and glucocorticoid stabilizes membrane‐associated actin in alveolar epithelial cells
In this study we found that hypoxia and dexamethasone (dex), a synthetic GC not only up‐regulated the expression of stomatin alone, but also imposed additive effect on the expression of stomatin in A549 cells, primary AEC and lung of rats. Then we investigated whether hypoxia and dex transcriptionally up‐regulated the expression of stomatin by reporter gene assay, and found that dex, but not hypoxia could increase the activity of a stomatin promoter‐driven reporter gene. Further deletion and mutational studies demonstrated that a GC response element (GRE) within the promoter region mainly contributed to the induction...
Source: Journal of Cellular and Molecular Medicine - May 15, 2013 Category: Molecular Biology Authors: Ji‐Cheng Chen, Hao‐Yu Cai, Yan Wang, Yuan‐Yuan Ma, Liang‐Nian Song, Li‐Juan Yin, Dong‐Mei Cao, Fei Diao, Yi‐Dong Li, Jian Lu Tags: Original Article Source Type: research

Crosstalk between hydrogen sulfide and nitric oxide in endothelial cells
Abstract Hydrogen sulfide (H2S) and nitric oxide (NO) are major gasotransmitters produced in endothelial cells (ECs), contributing to the regulation of vascular contractility and structural integrity. Their interaction at different levels would have a profound impact on angiogenesis. Here, we showed that H2S and NO stimulated the formation of new microvessels. Incubation of human umbilical vein endothelial cells (HUVECs‐926) with NaHS (a H2S donor) stimulated the phosphorylation of endothelial NO synthase (eNOS) and enhanced NO production. H2S had little effect on eNOS protein expression in ECs. L‐cysteine, a precursor...
Source: Journal of Cellular and Molecular Medicine - June 7, 2013 Category: Molecular Biology Authors: Zaid Altaany, Guangdong Yang, Rui Wang Tags: Original Article Source Type: research

Inhibition of checkpoint kinase 2 (CHK2) enhances sensitivity of pancreatic adenocarcinoma cells to gemcitabine
In this study, the combination treatment of NSC109555 plus GEM demonstrated strong synergistic antitumour effect in four pancreatic cancer cells (MIA PaCa‐2, CFPAC‐1, Panc‐1 and BxPC‐3). In addition, the GEM/NSC109555 combination significantly increased the level of intracellular reactive oxygen species (ROS), accompanied by induction of apoptotic cell death. Inhibition of ROS generation by N‐acetyl cysteine (NAC) significantly reversed the effect of GEM/NSC109555 in apoptosis and cytotoxicity. Furthermore, genetic knockdown of CHK2 by siRNA enhanced GEM‐induced apoptotic cell death. These findings suggest that...
Source: Journal of Cellular and Molecular Medicine - July 16, 2013 Category: Molecular Biology Authors: Hong‐Quan Duong, Young Bin Hong, Jung Soon Kim, Hee‐Seok Lee, Yong Weon Yi, Yeon Jeong Kim, Antai Wang, Wenjing Zhao, Chi Heum Cho, Yeon‐Sun Seong, Insoo Bae Tags: Original Article Source Type: research

Brucine suppresses colon cancer cells growth via mediating KDR signalling pathway
This study aimed to demonstrate the effect of brucine on tumour angiogenesis and its mechanism of action. The anti‐angiogenic effect was evaluated on the chicken chorioallantoic membrane (CAM) model and tube formation. The mechanism was demonstrated through detecting mRNA and protein expressions of VEGFR2 (KDR), PKCα, PLCγ and Raf1 by reverse transcription‐polymerase chain reaction (RT‐PCR) and Western blot (WB), as well as expressions of VEGF and PKCβ and mTOR by ELISA and WB. The results showed that brucine significantly reduced angiogenesis of CAM and tube formation, inhibited the VEGF secretion and mTOR expres...
Source: Journal of Cellular and Molecular Medicine - August 2, 2013 Category: Molecular Biology Authors: Wenjuan Luo, Xiaoli Wang, Lei Zheng, Yingzhuan Zhan, Dongdong Zhang, Jie Zhang, Yanmin Zhang Tags: Original Article Source Type: research

PPARγ silencing enhances osteogenic differentiation of human adipose‐derived mesenchymal stem cells
This study investigates the role of PPARγ in the lineage commitment of human adipose‐derived mesenchymal stem cells (hADSCs) by interfering with the function of PPARγ mRNA through small interfering RNAs (siRNAs) specific for PPARγ2. By applying an osteogenic induction condition less potent than that used conventionally, we found that PPARγ silencing led to retardation of adipogenesis and stimulated a higher level of matrix mineralization. The mRNA level of PPARγ decreased to 47% of control 2 days after treatment with 50 nmol/l PPARγ2 siRNA, while its protein expression was 60% of mock control. In the meantime, os...
Source: Journal of Cellular and Molecular Medicine - August 13, 2013 Category: Molecular Biology Authors: Mon‐Juan Lee, Hui‐Ting Chen, Mei‐Ling Ho, Chung‐Hwan Chen, Shu‐Chun Chuang, Sung‐Cheng Huang, Yin‐Chih Fu, Gwo‐Jaw Wang, Lin Kang, Je‐Ken Chang Tags: Short Communication Source Type: research

PAX3 in neuroblastoma: oncogenic potential, chemosensitivity and signalling pathways
Abstract Transcription factor PAX3/Pax3 contributes to diverse cell lineages during embryonic development and is important in tumourigenesis. We found that PAX3 is re‐expressed in neuroblastoma and malignant neuroblastic (N‐type) neuroblastoma cells had significantly higher PAX3 protein expression than their benign substrate‐adherent (S‐type) counterparts. Knock‐down of PAX3 expression by siRNA transfection resulted in persistent cell growth inhibition in both types of neuroblastoma cell, owing to G1 cell cycle arrest and progressive apoptosis. Inhibition of PAX3 expression significantly decreased the attachment ...
Source: Journal of Cellular and Molecular Medicine - November 4, 2013 Category: Molecular Biology Authors: Wen‐Hui Fang, Qiuyu Wang, Hong‐Mei Li, Mashud Ahmed, Patricia Kumar, Shant Kumar Tags: Original Article Source Type: research