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Source: Journal of Cellular and Molecular Medicine
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Total 94 results found since Jan 2013.
BMP‐9 regulates the osteoblastic differentiation and calcification of vascular smooth muscle cells through an ALK1 mediated pathway
Abstract
The process of vascular calcification shares many similarities with that of physiological skeletal mineralization, and involves the deposition of hydroxyapatite crystals in arteries. However, the cellular mechanisms responsible have yet to be fully explained. Bone morphogenetic protein (BMP‐9) has been shown to exert direct effects on both bone development and vascular function. In the present study, we have investigated the role of BMP‐9 in vascular smooth muscle cell (VSMC) calcification. Vessel calcification in chronic kidney disease (CKD) begins pre‐dialysis, with factors specific to the dialysis milieu ...
Source: Journal of Cellular and Molecular Medicine - October 9, 2014 Category: Molecular Biology Authors: Dongxing Zhu, Neil Charles Wallace Mackenzie, Catherine M. Shanahan, Rukshana C. Shroff, Colin Farquharson, Vicky Elisabeth MacRae Tags: Original Article Source Type: research
MiRNA‐30a inhibits AECs‐II apoptosis by blocking mitochondrial fission dependent on Drp‐1
This study demonstrated that miR‐30a inhibited AECs‐II apoptosis by repressing the mitochondrial fission dependent on Drp‐1, and could function as a novel therapeutic target for lung fibrosis.
Source: Journal of Cellular and Molecular Medicine - October 6, 2014 Category: Molecular Biology Authors: Cuiping Mao, Jinjin Zhang, Shengcui Lin, Lili Jing, Jing Xiang, Meirong Wang, Bingsi Wang, Pan Xu, Weili Liu, Xiaodong Song, Changjun Lv Tags: Original Article Source Type: research
ASPP2 attenuates triglycerides to protect against hepatocyte injury by reducing autophagy in a cell and mouse model of non‐alcoholic fatty liver disease
In conclusion, ASPP2 may participate in the lipid metabolism of non‐alcoholic steatohepatitis and attenuate liver failure.
Source: Journal of Cellular and Molecular Medicine - September 25, 2014 Category: Molecular Biology Authors: Fang Xie, Lin Jia, Minghua Lin, Ying Shi, Jiming Yin, Yin Liu, Dexi Chen, Qinghua Meng Tags: Original Article Source Type: research
Elongation factor‐2 kinase regulates TG2/β1 integrin/Src/uPAR pathway and epithelial–mesenchymal transition mediating pancreatic cancer cells invasion
Abstract
Pancreatic ductal adenocarcinoma is one of the lethal cancers with extensive local tumour invasion, metastasis, early systemic dissemination and poorest prognosis. Thus, understanding the mechanisms regulating invasion/metastasis and epithelial–mesenchymal transition (EMT), is the key for developing effective therapeutic strategies for pancreatic cancer (PaCa). Eukaryotic elongation factor‐2 kinase (eEF‐2K) is an atypical kinase that we found to be highly up‐regulated in PaCa cells. However, its role in PaCa invasion/progression remains unknown. Here, we investigated the role of eEF‐2K in cellular invasi...
Source: Journal of Cellular and Molecular Medicine - September 12, 2014 Category: Molecular Biology Authors: Ahmed A. Ashour, Nilgun Gurbuz, Sultan Neslihan Alpay, Abdel‐Aziz H. Abdel‐Aziz, Ahmed M. Mansour, Longfei Huo, Bulent Ozpolat Tags: Original Article Source Type: research
KITLG is a novel target of miR‐34c that is associated with the inhibition of growth and invasion in colorectal cancer cells
In conclusion, our results demonstrated that miR‐34c might interfere with KITLG‐related CRC and could be a novel molecular target for CRC patients.
Source: Journal of Cellular and Molecular Medicine - September 12, 2014 Category: Molecular Biology Authors: Shu Yang, Wen‐shuai Li, Fang Dong, Hai‐mei Sun, Bo Wu, Jun Tan, Wan‐jing Zou, De‐shan Zhou Tags: Original Article Source Type: research
Resveratrol‐enhanced autophagic flux ameliorates myocardial oxidative stress injury in diabetic mice
Abstract
Autophagic dysfunction is observed in diabetes mellitus. Resveratrol has a beneficial effect on diabetic cardiomyopathy. Whether the resveratrol‐induced improvement in cardiac function in diabetes is via regulating autophagy remains unclear. We investigated the mechanisms underlying resveratrol‐mediated protection against heart failure in diabetic mice, with a focus on the role of sirtuin 1 (SIRT1) in regulating autophagic flux. Diabetic cardiomyopathy in mice was induced by streptozotocin (STZ). Long‐term resveratrol treatment improved cardiac function, ameliorated oxidative injury and reduced apoptosis in ...
Source: Journal of Cellular and Molecular Medicine - June 1, 2014 Category: Molecular Biology Authors: Bo Wang, Qing Yang, Yuan‐yuan Sun, Yi‐fan Xing, Ying‐bin Wang, Xiao‐ting Lu, Wen‐wu Bai, Xiao‐qiong Liu, Yu‐xia Zhao Tags: Original Article Source Type: research
PMC, a potent hydrophilic α‐tocopherol derivative, inhibits NF‐κB activation via PP2A but not IκBα‐dependent signals in vascular smooth muscle cells
In this study, we investigated the mechanisms of the inhibitory effects of PMC in vascular smooth muscle cells (VSMCs) exposed to pro‐inflammatory stimuli, lipopolysaccharide (LPS) combined with interferon (IFN)‐γ. Treatment of LPS/IFN‐γ‐stimulated VSMCs with PMC suppressed the expression of inducible nitric oxide synthase (iNOS) and matrix metalloproteinase‐9 in a concentration‐dependent manner. A reduction in LPS/IFN‐γ‐induced nuclear factor (NF)‐κB activation was also observed in PMC‐treated VSMCs. The translocation and phosphorylation of p65, protein phosphatase 2A (PP2A) inactivation and the fo...
Source: Journal of Cellular and Molecular Medicine - April 13, 2014 Category: Molecular Biology Authors: Cheng‐Ying Hsieh, George Hsiao, Ming‐Jen Hsu, Yi‐Hsuan Wang, Joen‐Rong Sheu Tags: Original Article Source Type: research
Overexpression of STAMP2 suppresses atherosclerosis and stabilizes plaques in diabetic mice
Abstract
Our research aims to evaluate the function of the STAMP2 gene, an important trigger in insulin resistance (IR), and explore its role in macrophage apoptosis in diabetic atherosclerotic vulnerable plaques. The characteristics of diabetic mice were measured by serial metabolite and pathology tests. The level of STAMP2 was measured by RT‐PCR and Western blot. The plaque area, lipid and collagen content of brachiocephalic artery plaques were measured by histopathological analyses, and the macrophage apoptosis was measured by TUNEL. Correlation of STAMP2/Akt signaling pathway and macrophage apoptosis was validated by...
Source: Journal of Cellular and Molecular Medicine - January 22, 2014 Category: Molecular Biology Authors: Jia Wang, Lu Han, Zhi‐hao Wang, Wen‐yuan Ding, Yuan‐yuan Shang, Meng‐xiong Tang, Wen‐bo Li, Yun Zhang, Wei Zhang, Ming Zhong Tags: Original Article Source Type: research
Apelin inhibits the proliferation and migration of rat PASMCs via the activation of PI3K/Akt/mTOR signal and the inhibition of autophagy under hypoxia
This study provides the evidence that exogenous apelin treatment contributes to inhibit the proliferation and migration of PASMCs by regulating the level of autophagy.
Source: Journal of Cellular and Molecular Medicine - January 22, 2014 Category: Molecular Biology Authors: Hongyu Zhang, Yongsheng Gong, Zhouguang Wang, Liping Jiang, Ran Chen, Xiaofang Fan, Huanmian Zhu, Liping Han, Xiaokun Li, Jian Xiao, Xiaoxia Kong Tags: Original Article Source Type: research
CD34+VEGFR‐3+ progenitor cells have a potential to differentiate towards lymphatic endothelial cells
Abstract
Endothelial progenitor cells (EPCs) play an important role in postnatal neovascularization. However, it is poorly understood whether EPCs contribute to lymphangiogenesis. Here, we assessed differentiation of a noval population of EPCs towards lymphatic endothelial cells and their lymphatic formation. CD34+VEGFR‐3+ EPCs were isolated from mononuclear cells of human cord blood by fluorescence‐activated cell sorting. These cells expressed CD133 and displayed the phenotype of the endothelial cells. Cell colonies appeared at 7–10 days after incubation. The cells of the colonies grew rapidly and could be repeated...
Source: Journal of Cellular and Molecular Medicine - January 22, 2014 Category: Molecular Biology Authors: Yu‐zhen Tan, Hai‐jie Wang, Mei‐hua Zhang, Zhe Quan, Ting Li, Qi‐zhi He Tags: Original Article Source Type: research
Cordycepin activates AMP‐activated protein kinase (AMPK) via interaction with the γ1 subunit
Abstract
Cordycepin is a bioactive component of the fungus Cordyceps militaris. Previously, we showed that cordycepin can alleviate hyperlipidemia through enhancing the phosphorylation of AMP‐activated protein kinase (AMPK), but the mechanism of this stimulation is unknown. Here, we investigated the potential mechanisms of cordycepin‐induced AMPK activation in HepG2 cells. Treatment with cordycepin largely reduced oleic acid (OA)‐elicited intracellular lipid accumulation and increased AMPK activity in a dose‐dependent manner. Cordycepin‐induced AMPK activation was not accompanied by changes in either the intracel...
Source: Journal of Cellular and Molecular Medicine - November 28, 2013 Category: Molecular Biology Authors: Chongming Wu, Yanshen Guo, Yan Su, Xue Zhang, Hong Luan, Xiaopo Zhang, Huixin Zhu, Huixia He, Xiaoliang Wang, Guibo Sun, Xiaobo Sun, Peng Guo, Ping Zhu Tags: Original Article Source Type: research
PAX3 in neuroblastoma: oncogenic potential, chemosensitivity and signalling pathways
Abstract
Transcription factor PAX3/Pax3 contributes to diverse cell lineages during embryonic development and is important in tumourigenesis. We found that PAX3 is re‐expressed in neuroblastoma and malignant neuroblastic (N‐type) neuroblastoma cells had significantly higher PAX3 protein expression than their benign substrate‐adherent (S‐type) counterparts. Knock‐down of PAX3 expression by siRNA transfection resulted in persistent cell growth inhibition in both types of neuroblastoma cell, owing to G1 cell cycle arrest and progressive apoptosis. Inhibition of PAX3 expression significantly decreased the attachment ...
Source: Journal of Cellular and Molecular Medicine - November 4, 2013 Category: Molecular Biology Authors: Wen‐Hui Fang, Qiuyu Wang, Hong‐Mei Li, Mashud Ahmed, Patricia Kumar, Shant Kumar Tags: Original Article Source Type: research
PPARγ silencing enhances osteogenic differentiation of human adipose‐derived mesenchymal stem cells
This study investigates the role of PPARγ in the lineage commitment of human adipose‐derived mesenchymal stem cells (hADSCs) by interfering with the function of PPARγ mRNA through small interfering RNAs (siRNAs) specific for PPARγ2. By applying an osteogenic induction condition less potent than that used conventionally, we found that PPARγ silencing led to retardation of adipogenesis and stimulated a higher level of matrix mineralization. The mRNA level of PPARγ decreased to 47% of control 2 days after treatment with 50 nmol/l PPARγ2 siRNA, while its protein expression was 60% of mock control. In the meantime, os...
Source: Journal of Cellular and Molecular Medicine - August 13, 2013 Category: Molecular Biology Authors: Mon‐Juan Lee, Hui‐Ting Chen, Mei‐Ling Ho, Chung‐Hwan Chen, Shu‐Chun Chuang, Sung‐Cheng Huang, Yin‐Chih Fu, Gwo‐Jaw Wang, Lin Kang, Je‐Ken Chang Tags: Short Communication Source Type: research
Brucine suppresses colon cancer cells growth via mediating KDR signalling pathway
This study aimed to demonstrate the effect of brucine on tumour angiogenesis and its mechanism of action. The anti‐angiogenic effect was evaluated on the chicken chorioallantoic membrane (CAM) model and tube formation. The mechanism was demonstrated through detecting mRNA and protein expressions of VEGFR2 (KDR), PKCα, PLCγ and Raf1 by reverse transcription‐polymerase chain reaction (RT‐PCR) and Western blot (WB), as well as expressions of VEGF and PKCβ and mTOR by ELISA and WB. The results showed that brucine significantly reduced angiogenesis of CAM and tube formation, inhibited the VEGF secretion and mTOR expres...
Source: Journal of Cellular and Molecular Medicine - August 2, 2013 Category: Molecular Biology Authors: Wenjuan Luo, Xiaoli Wang, Lei Zheng, Yingzhuan Zhan, Dongdong Zhang, Jie Zhang, Yanmin Zhang Tags: Original Article Source Type: research
Inhibition of checkpoint kinase 2 (CHK2) enhances sensitivity of pancreatic adenocarcinoma cells to gemcitabine
In this study, the combination treatment of NSC109555 plus GEM demonstrated strong synergistic antitumour effect in four pancreatic cancer cells (MIA PaCa‐2, CFPAC‐1, Panc‐1 and BxPC‐3). In addition, the GEM/NSC109555 combination significantly increased the level of intracellular reactive oxygen species (ROS), accompanied by induction of apoptotic cell death. Inhibition of ROS generation by N‐acetyl cysteine (NAC) significantly reversed the effect of GEM/NSC109555 in apoptosis and cytotoxicity. Furthermore, genetic knockdown of CHK2 by siRNA enhanced GEM‐induced apoptotic cell death. These findings suggest that...
Source: Journal of Cellular and Molecular Medicine - July 16, 2013 Category: Molecular Biology Authors: Hong‐Quan Duong, Young Bin Hong, Jung Soon Kim, Hee‐Seok Lee, Yong Weon Yi, Yeon Jeong Kim, Antai Wang, Wenjing Zhao, Chi Heum Cho, Yeon‐Sun Seong, Insoo Bae Tags: Original Article Source Type: research
