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Source: Journal of Cellular and Molecular Medicine
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Total 94 results found since Jan 2013.
The lncRNA HNF1A ‐AS1 is a negative prognostic factor and promotes tumorigenesis in osteosarcoma
In this study, we detected the HNF1A‐AS1 levels in human osteosarcoma tissues and cell lines by quantitative real‐time polymerase chain reaction (qRT‐PCR), and investigated its role in osteosarcoma by using in vitro assays. Our study showed that HNF1A‐AS1 expression was significantly up‐regulated in human osteosarcoma tissues and cell lines compared with their normal counterparts, and its expression level was positively correlated with the distance metastasis (P = 0.009) and tumour stage (P = 0.019). Moreover, Kaplan–Meier curves with the log‐rank test showed that higher expression of HNF1A‐AS1 conferred a ...
Source: Journal of Cellular and Molecular Medicine - September 1, 2017 Category: Molecular Biology Authors: Lijun Cai, Jinhan Lv, Yinquan Zhang, Junhong Li, Yinong Wang, Huilin Yang Tags: Original Article Source Type: research
Low expression of SEMA6C accelerates the primordial follicle activation in the neonatal mouse ovary
In this study, we aimed to study whether and how semaphorin 6C (Sema6c) regulated the primordial follicle activation in the neonatal mouse ovary. The attenuation of SEMA6C expression by SiRNA accelerated the primordial follicle activation in the in vitro ovary culture system. PI3K‐AKT‐rpS6 pathway was activated when SEMA6C expression was down‐regulated. And the LY294002 could reverse the effect of low SEMA6C expression on primordial follicle activation. Our findings revealed that Sema6c was involved in the activation of primordial follicles, and the down‐regulation of SEMA6C led to massive primordial follicle acti...
Source: Journal of Cellular and Molecular Medicine - September 1, 2017 Category: Molecular Biology Authors: Su Zhou, Wei Yan, Wei Shen, Jing Cheng, Yueyue Xi, Suzhen Yuan, Fangfang Fu, Ting Ding, Aiyue Luo, Shixuan Wang Tags: Original Article Source Type: research
Silencing of CA1 mRNA in tumour cells does not change the gene expression of the extracellular matrix proteins
We report the silencing of CA1 mRNA in PC3 and MDA cells. The levels of mRNA coding CA1 protein in the knock‐down mRNA (CA1 siRNA) cells have been measured by RT‐PCR and were approximately 5% (PC3) and 20% (MDA‐MB‐231), respectively, of the level of control (Mock siRNA) used during silencing. In PC3 and MDA‐MB‐231 cells, the mRNAs for COL1A1 and COL4A4 were up‐regulated. The mRNAs for CTHRC1, LAMC2, and WNT7B were not changed when compared to the control. The morphology of the cells during the treatments remained the same. On the Western blots, the lysate from the silenced cells showed lower levels of CA I as well.
Source: Journal of Cellular and Molecular Medicine - August 7, 2017 Category: Molecular Biology Authors: Radivojka Vulic, Silvia Tyciakova, Maria Dubrovcakova, Ludovit Skultety, Jan Lakota Tags: Short Communication Source Type: research
Down ‐regulation of miR‐10a‐5p in synoviocytes contributes to TBX5‐controlled joint inflammation
Abstract
MicroRNAs are considered to play critical roles in the pathogenesis of human inflammatory arthritis, including rheumatoid arthritis (RA). The purpose of this study was to determine the relationship between miR‐10a‐5p and TBX5 in synoviocytes and evaluate their contribution to joint inflammation. The expression of miR‐10a‐5p and TBX5 in the synovium of RA and human synovial sarcoma cell line SW982 stimulated by IL‐1β was determined by RT‐qPCR and Western blotting. The direct interaction between miR‐10a‐5p and TBX5 3′UTR was determined by dual‐luciferase reporter assay in HeLa cells. Mimics and ...
Source: Journal of Cellular and Molecular Medicine - August 7, 2017 Category: Molecular Biology Authors: Nazim Hussain, Wenhua Zhu, Congshan Jiang, Jing Xu, Xiaoying Wu, Manman Geng, Safdar Hussain, Yongsong Cai, Ke Xu, Peng Xu, Yan Han, Jian Sun, Liesu Meng, Shemin Lu Tags: Original Article Source Type: research
LINC01101 and LINC00277 expression levels as novel factors in HPV ‐induced cervical neoplasia
Abstract
Recently long non‐coding RNAs were identified as new factors involved in gene expression regulation. To gain insight into expression pattern of these factors related to E7 HPV18 oncogene, this study uses HeLa cell culture transfected with E7‐siRNA. Gene expression profile was investigated using microarray analysis. After analysing the microarray results, we identified 15,387 RNA species differentially expressed in E7‐siRNA‐transfected cells compared with controls (fold change >2). The expression profiles of lncRNA species highlighted 731 lncRNAs and 203 lincRNAs. We selected two lincRNAs (LINC01101 and ...
Source: Journal of Cellular and Molecular Medicine - August 2, 2017 Category: Molecular Biology Authors: Iulia Virginia Iancu, Gabriela Anton, Anca Botezatu, Irina Huica, Anca Nastase, Demetra Gabriela Socolov, Anca Daniela Stanescu, Simona Olimpia Dima, Nicolae Bacalbasa, Adriana Plesa Tags: Original Article Source Type: research
Propofol post ‐conditioning alleviates hepatic ischaemia reperfusion injury via BRG1‐mediated Nrf2/HO‐1 transcriptional activation in human and mice
Abstract
To explore the effects of propofol post‐conditioning (PPC) on hepatic ischaemia/reperfusion injury (HIRI) and the potential mechanisms that might be involved in the interaction of Brahma‐related gene1(BRG1) and Nuclear‐related factor 2(Nrf2). Patients were randomized into PPC(n = 16) and non‐PPC(NPC)( n = 21) groups. Propofol(2 mg/kg) was infused within 10 min. of the onset of liver reperfusion during liver transplantation in the PPC group. Liver function tests, as well as Brg1, Nrf2, Heme oxygenase‐1(HO‐1) and NADPH:quinone oxidoreductase1(NQO1) expression levels were evaluated. CMV‐Brg1 mice were d...
Source: Journal of Cellular and Molecular Medicine - July 1, 2017 Category: Molecular Biology Authors: Mian Ge, Huixin Chen, Qianqian Zhu, Jun Cai, Chaojin Chen, Dongdong Yuan, Yi Jin, Weifeng Yao, Ziqing Hei Tags: Original Article Source Type: research
The apolipoprotein A ‐I mimetic peptide, D‐4F, restrains neointimal formation through heme oxygenase‐1 up‐regulation
In conclusion, D‐4F inhibited VSMC proliferation and migration in vitro and neointimal formation in vivo through HO‐1 up‐regulation, which provided a novel prophylactic and therapeutic strategy for anti‐restenosis of arteries.
Source: Journal of Cellular and Molecular Medicine - July 1, 2017 Category: Molecular Biology Authors: Donghui Liu, Mengzhang Wu, Qian Du, Zhenzhen Ding, Mingming Qian, Zijia Tong, Wenqi Xu, Le Zhang, He Chang, Yan Wang, Caihua Huang, Donghai Lin Tags: Original Article Source Type: research
MicroRNA ‐30a ameliorates hepatic fibrosis by inhibiting Beclin1‐mediated autophagy
In conclusion, miR‐30a is down‐regulated in hepatic fibrosis models and its overexpression prevents liver fibrogenesis by directly suppressing Beclin1‐mediated autophagy; therefore, miR‐30a may be a new potential therapeutic target for controlling hepatic fibrosis.
Source: Journal of Cellular and Molecular Medicine - July 1, 2017 Category: Molecular Biology Authors: Jianliang Chen, Yue Yu, Shu Li, Yuting Liu, Shu Zhou, Shouji Cao, Jie Yin, Guoqiang Li Tags: Original Article Source Type: research
Catalase ameliorates diabetes ‐induced cardiac injury through reduced p65/RelA‐ mediated transcription of BECN1
Abstract
Catalase is an antioxidative enzyme that converts hydrogen peroxide (H2O2) produced by superoxide dismutase from highly reactive superoxide (O2−) to water and oxygen molecules. Although recent findings demonstrate that catalase, autophagy and the nuclear factor κB (NF‐κB) signalling pathway are centrally involved in diabetic cardiomyopathy (DCM), the interplay between the three has not been fully characterized. Thus, the mechanism responsible for catalase‐mediated protection against heart injury in diabetic mice was investigated in this study, as well as the role of NF‐κB‐p65 in the regulation of auto...
Source: Journal of Cellular and Molecular Medicine - June 23, 2017 Category: Molecular Biology Authors: Xu Wang, Youli Tao, Yewei Huang, Kungao Zhan, Mei Xue, Ying Wang, Dandan Ruan, Yangzhi Liang, Xiaozhong Huang, Jianjun Lin, Zhiwei Chen, Lingchun Lv, Santie Li, Gen Chen, Yang Wang, Ruijie Chen, Weitao Cong, Litai Jin Tags: Original Article Source Type: research
Overexpressing STAMP2 attenuates adipose tissue angiogenesis and insulin resistance in diabetic ApoE −/−/LDLR−/− mouse via a PPARγ/CD36 pathway
Abstract
The aim of this study was to investigate whether overexpression of STAMP2 improves insulin resistance by regulating angiogenesis in adipose tissues. The characteristics of diabetic mice were measured by serial metabolite and pathology tests. Samples were obtained from epididymal, subcutaneous and brown adipose tissues. Histological and morphological analysis demonstrated that STAMP2 gene overexpression reduced adipocyte size, angiogenesis in epididymal and brown adipose tissues. On aortic ring assay, microvessels sprouting from aortas were significantly inhibited after STAMP2 gene overexpression. The cellular effe...
Source: Journal of Cellular and Molecular Medicine - June 19, 2017 Category: Molecular Biology Authors: Feng Wang, Lu Han, Ran ‐ran Qin, Yao‐yuan Zhang, Di Wang, Zhi‐Hao Wang, Meng‐Xiong Tang, Yun Zhang, Ming Zhong, Wei Zhang Tags: Original Article Source Type: research
Hydrogen Sulphide modulating mitochondrial morphology to promote mitophagy in endothelial cells under high ‐glucose and high‐palmitate
Abstract
Endothelial cell dysfunction is one of the main reasons for type II diabetes vascular complications. Hydrogen sulphide (H2S) has antioxidative effect, but its regulation on mitochondrial dynamics and mitophagy in aortic endothelial cells under hyperglycaemia and hyperlipidaemia is unclear. Rat aortic endothelial cells (RAECs) were treated with 40 mM glucose and 200 μM palmitate to imitate endothelium under hyperglycaemia and hyperlipidaemia, and 100 μM NaHS was used as an exogenous H2S donor. Firstly, we demonstrated that high glucose and palmitate decreased H2S production and CSE expression in RAECs. Then, the ...
Source: Journal of Cellular and Molecular Medicine - June 13, 2017 Category: Molecular Biology Authors: Ning Liu, Jichao Wu, Linxue Zhang, Zhaopeng Gao, Yu Sun, Miao Yu, Yajun Zhao, Shiyun Dong, Fanghao Lu, Weihua Zhang Tags: Original Article Source Type: research
Dual inhibition of BDNF/TrkB and autophagy: a promising therapeutic approach for colorectal cancer
Abstract
Colorectal cancer (CRC) is the most common digestive cancer in the Western world. Despite effective therapies, resistance and/or recurrence frequently occur. The present study investigated the impact of two survival pathways—neurotrophic factors (TrkB/BDNF) and autophagy—on cell fate and tumour evolution. In vitro studies were performed on two CRC cell lines, SW480 (primary tumour) and SW620 (lymph node invasion), which were also used for subcutaneous xenografts on a nude mouse model. In addition, the presence of neurotrophic factors (NTs) and autophagy markers were assessed in tissue samples representative of...
Source: Journal of Cellular and Molecular Medicine - June 9, 2017 Category: Molecular Biology Authors: Cl ément Mazouffre, Sophie Geyl, Aurélie Perraud, Sabrina Blondy, Marie‐Odile Jauberteau, Muriel Mathonnet, Mireille Verdier Tags: Original Article Source Type: research
Cimifugin suppresses allergic inflammation by reducing epithelial derived initiative key factors via regulating tight junctions
This study aims to determine the mechanism of cimifugin on epithelial cells in allergic inflammation. Mice were sensitized and challenged with FITC to establish type 2 atopic dermatitis (AD) model. The initial stage of AD model, in which mice were just sensitized with FITC, was established in vivo and immortalized human epidermal (HaCaT) cells were utilized in vitro. Initiative key cytokines, TSLP and IL‐33, were measured by ELISA, the junctions in ECs were observed by electron microscopy and TJs (CLDN‐1, occludin and CLDND1) were assessed by Western blot, immunohistochemistry and immunofluorescence. The results show...
Source: Journal of Cellular and Molecular Medicine - June 9, 2017 Category: Molecular Biology Authors: Xiaoyu Wang, Xiaoyan Jiang, Xi Yu, Hailiang Liu, Yu Tao, Guorong Jiang, Min Hong Tags: Original Article Source Type: research
Targeting YAP in malignant pleural mesothelioma
In this study, we evaluated the effect of targeting YAP in mesothelioma. First, we comprehensively studied YAP activity in five mesothelioma cell lines (211H, H2052, H290, MS‐1 and H2452) and one normal mesothelial cell line (LP9). We found decreased phospho‐YAP to YAP protein ratio and consistently increased GTIIC reporter activity in 211H, H2052 and H290 compared to LP9. The same three cell lines (IC50s < 1 μM) were more sensitive than LP9 (IC50 = 3.5 μM) to the YAP/TEAD inhibitor verteporfin. We also found that verteporfin significantly reduced YAP protein level, mRNA levels of YAP downstream genes and GTIIC re...
Source: Journal of Cellular and Molecular Medicine - May 4, 2017 Category: Molecular Biology Authors: Wen ‐Qian Zhang, Yu‐Yuan Dai, Ping‐Chih Hsu, Hui Wang, Li Cheng, Yi‐Lin Yang, Yu‐Cheng Wang, Zhi‐Dong Xu, Shu Liu, Geraldine Chan, Bin Hu, Hui Li, David M. Jablons, Liang You Tags: Original Article Source Type: research
LncRNA MALAT1 is dysregulated in diabetic nephropathy and involved in high glucose ‐induced podocyte injury via its interplay with β‐catenin
In this study, we found that MALAT1 levels were increased in kidney cortices from C57BL/6 mice with streptozocin (STZ)‐induced diabetic nephropathy, and dynamically regulated in cultured mouse podocytes stimulated with high glucose, which showed a trend from rise to decline. The decline of MALAT1 levels was accompanied with β‐catenin translocation to the nuclei and enhanced expression of serine/arginine splicing factor 1 (SRSF1), a MALAT1 RNA‐binding protein. Further we showed early interference with MALAT1 siRNA partially restored podocytes function and prohibited β‐catenin nuclear accumulation and SRSF1 overexp...
Source: Journal of Cellular and Molecular Medicine - April 26, 2017 Category: Molecular Biology Authors: Mengsi Hu, Rong Wang, Xiaobing Li, Minghua Fan, Jiangong Lin, Junhui Zhen, Liqun Chen, Zhimei Lv Tags: Original Article Source Type: research
