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Source: Journal of Cellular and Molecular Medicine

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Total 94 results found since Jan 2013.

Cul4A overexpression associated with Gli1 expression in malignant pleural mesothelioma
In this study, we analysed clinical mesothelioma tumours and found moderate to strong expression of Cul4A in 70.9% (51/72) of these tumours, as shown by immunohistochemistry. In 72.2% mesothelioma tumours with increased Cul4A copy number identified by fluorescence in situ hybridization analysis, Cul4A protein expression was moderate to strong. Similarly, Cul4A was overexpressed and Cul4A copy number was increased in human mesothelioma cell lines. Because Gli1 is highly expressed in human mesothelioma cells, we compared Cul4A and Gli1 expression in mesothelioma tumours and found their expression associated (P < 0.05, ...
Source: Journal of Cellular and Molecular Medicine - July 27, 2015 Category: Molecular Biology Authors: Yi‐Lin Yang, Jian Ni, Ping‐Chih Hsu, Jian‐Hua Mao, David Hsieh, Angela Xu, Geraldine Chan, Alfred Au, Zhidong Xu, David M. Jablons, Liang You Tags: Original Article Source Type: research

A novel TXNIP‐based mechanism for Cx43‐mediated regulation of oxidative drug injury
Abstract Gap junctions (GJs) play an important role in the regulation of cell response to many drugs. However, little is known about their mechanisms. Using an in vitro model of cytotoxicity induced by geneticin (G418), we explored the potential signalling mechanisms involved. Incubation of cells with G418 resulted in cell death, as indicated by the change in cell morphology, loss of cell viability and activation of caspase‐3. Before the onset of cell injury, G418 induced reactive oxygen species (ROS) generation, activated oxidative sensitive kinase P38 and caused a shift of connexin 43 (Cx43) from non‐phosphorylated ...
Source: Journal of Cellular and Molecular Medicine - July 8, 2015 Category: Molecular Biology Authors: Kun Gao, Yuan Chi, Xiling Zhang, Hui Zhang, Gang Li, Wei Sun, Masayuki Takeda, Jian Yao Tags: Original Article Source Type: research

Histone deacetylase 5 regulates the inflammatory response of macrophages
Abstract Modifying the chromatin structure and interacting with non‐histone proteins, histone deacetylases (HDAC) are involved in vital cellular processes at different levels. We here specifically investigated the direct effects of HDAC5 in macrophage activation in response to bacterial or cytokine stimuli. Using murine and human macrophage cell lines, we studied the expression profile and the immunological function of HDAC5 at transcription and protein level in over‐expression as well as RNA interference experiments. Toll‐like receptor‐mediated stimulation of murine RAW264.7 cells significantly reduced HDAC5 mRNA ...
Source: Journal of Cellular and Molecular Medicine - June 8, 2015 Category: Molecular Biology Authors: Lukas Poralla, Thorsten Stroh, Ulrike Erben, Marie Sittig, Sven Liebig, Britta Siegmund, Rainer Glauben Tags: Original Article Source Type: research

Identification of C‐terminal Hsp70‐interacting protein as a mediator of tumour necrosis factor action in osteoblast differentiation by targeting osterix for degradation
Abstract In patients with inflammatory arthritis, tumour necrosis factor (TNF)‐α are overproduced in inflamed joints. This leads to local erosion of cartilage and bone, periarticular osteopenia, as well as osteoporosis. But less is known regarding the molecular mechanisms that mediate the effect of TNF‐α on osteoblast function. The purpose of this study was to test that C terminus of Hsc70‐interacting protein (CHIP) has a specific role in suppressing the osteogenic activity of osteoblasts under inflammatory conditions. C2C12, MC3T3‐E1 and HEK293T cell lines were cultured and cotransfected with related plasmids. A...
Source: Journal of Cellular and Molecular Medicine - March 26, 2015 Category: Molecular Biology Authors: Jianmin Xie, Jieruo Gu Tags: Original Article Source Type: research

Long non‐coding RNA MALAT1 regulates hyperglycaemia induced inflammatory process in the endothelial cells
Abstract To examine whether the long non‐coding RNA (lncRNA) metastasis associated lung adenocarcinoma transcript 1 (MALAT1) is altered in the endothelial cells in response to glucose and the significance of such alteration. We incubated human umbilical vein endothelial cells with media containing various glucose levels. We found an increase in MALAT1 expression peaking after 12 hrs of incubation in high glucose. This increase was associated with parallel increase in serum amyloid antigen 3 (SAA3), an inflammatory ligand and target of MALAT1 and was further accompanied by increase in mRNAs and proteins of inflammatory m...
Source: Journal of Cellular and Molecular Medicine - March 19, 2015 Category: Molecular Biology Authors: Prasanth Puthanveetil, Shali Chen, Biao Feng, Anirudh Gautam, Subrata Chakrabarti Tags: Original Article Source Type: research

Changes in short‐chain acyl‐coA dehydrogenase during rat cardiac development and stress
This study was designed to investigate the expression of short‐chain acyl‐CoA dehydrogenase (SCAD), a key enzyme of fatty acid β‐oxidation, during rat heart development and the difference of SCAD between pathological and physiological cardiac hypertrophy. The expression of SCAD was lowest in the foetal and neonatal heart, which had time‐dependent increase during normal heart development. In contrast, a significant decrease in SCAD expression was observed in different ages of spontaneously hypertensive rats (SHR). On the other hand, swim‐trained rats developed physiological cardiac hypertrophy, whereas SHR develo...
Source: Journal of Cellular and Molecular Medicine - March 8, 2015 Category: Molecular Biology Authors: Jinxian Huang, Lipeng Xu, Qiuju Huang, Jiani Luo, Peiqing Liu, Shaorui Chen, Xi Yuan, Yao Lu, Ping Wang, Sigui Zhou Tags: Original Article Source Type: research

TLR3/TRIF signalling pathway regulates IL‐32 and IFN‐β secretion through activation of RIP‐1 and TRAF in the human cornea
In this study, we investigated how the TLR3 and RIG‐I signalling pathway was stimulated by viral infection to produce interleukin (IL)‐32‐mediated pro‐inflammatory cytokines and type I interferon in the corneal epithelium using Epstein–Barr virus (EBV)‐infected human cornea epithelial cells (HCECs/EBV) as a model of viral keratitis. Increased TLR3 and RIG‐I that are responded to EBV‐encoded RNA 1 and 2 (EBER1 and EBER2) induced the secretion of IL‐32‐mediated pro‐inflammatory cytokines and IFN‐β through up‐regulation of TRIF/TRAF family proteins or RIP‐1. TRIF silencing or TLR3 inhibitors more ...
Source: Journal of Cellular and Molecular Medicine - March 6, 2015 Category: Molecular Biology Authors: Ga Bin Park, Dae Young Hur, Yeong Seok Kim, Hyun‐Kyung Lee, Jae Wook Yang, Daejin Kim Tags: Original Article Source Type: research

Qiliqiangxin inhibits angiotensin II‐induced transdifferentiation of rat cardiac fibroblasts through suppressing interleukin‐6
This study aimed to investigate the effects of QL on angiotensin II (AngII)‐induced CFs transdifferentiation. Study was performed on in vitro cultured CFs from Sprague–Dawley rats. CFs differentiation was induced by AngII, which was attenuated by QL through reducing transforming growth factor‐β1 (TGF‐β1) and α‐smooth muscle actin (α‐SMA). Our data showed that AngII‐induced IL‐6 mRNA as well as typeI and typeIII collagens were reduced by QL. IL‐6 deficiency could suppress TGF‐β1 and α‐SMA, and both IL‐6 siRNA and QL‐mediated such effect was reversed by foresed expression of recombined IL‐6....
Source: Journal of Cellular and Molecular Medicine - March 6, 2015 Category: Molecular Biology Authors: Jingmin Zhou, Kun Jiang, Xuefeng Ding, Mingqiang Fu, Shijun Wang, Lingti Zhu, Tao He, Jingfeng Wang, Aijun Sun, Kai Hu, Li Chen, Yunzeng Zou, Junbo Ge Tags: Original Article Source Type: research

Lin28a protects against cardiac ischaemia/reperfusion injury in diabetic mice through the insulin‐PI3K‐mTOR pathway
This study indicates that lin28a overexpression reduces IS, improves cardiac function, decreases cardiomyocyte apoptosis index and alleviates cardiomyocyte mitochondria impairment after cardiac I/R injury in diabetic mice. The mechanism responsible for the effects of lin28a is associated with the insulin‐PI3K‐mTOR dependent pathway.
Source: Journal of Cellular and Molecular Medicine - February 16, 2015 Category: Molecular Biology Authors: Mingming Zhang, Dongdong Sun, Shuang Li, Xietian Pan, Xiaotian Zhang, Di Zhu, Congye Li, Rongqing Zhang, Erhe Gao, Haichang Wang Tags: Original Article Source Type: research

Overexpression of deubiquitinating enzyme USP28 promoted non‐small cell lung cancer growth
Abstract Non‐small cell lung cancer (NSCLC) accounts for most lung cancer. To develop new therapy required the elucidation of NSCLC pathogenesis. The deubiquitinating enzymes USP 28 has been identified and studied in colon and breast carcinomas. However, the role of USP28 in NSCLC is unknown. The level mRNA or protein level of USP28 were measured by qRT‐PCR or immunohistochemistry (IHC). The role of USP28 in patient survival was revealed by Kaplan–Meier plot of overall survival in NSCLC patients. USP28 was up or down regulated by overexpression plasmid or siRNA transfection. Cell proliferation and apoptosis was assay...
Source: Journal of Cellular and Molecular Medicine - February 5, 2015 Category: Molecular Biology Authors: Lei Zhang, Biao Xu, Yong Qiang, Hairong Huang, Changtian Wang, Demin Li, Jianjun Qian Tags: Original Article Source Type: research

Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin‐resistant uterine cancer
In this study, low‐abundant mitochondrial proteins were enriched for proteomic analysis with the state‐of‐the‐art two‐dimensional differential gel electrophoresis (2D‐DIGE) and matrix‐assistant laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) strategy to compare and identify the mitochondrial protein profiling changes in response to the development of doxorubicin resistance in human uterine cancer cells. The mitochondrial proteomic results demonstrate more than fifteen hundred protein features were resolved from the equal amount pooled of three purified mitochondrial proteins ...
Source: Journal of Cellular and Molecular Medicine - January 30, 2015 Category: Molecular Biology Authors: Yi‐Wen Lo, Szu‐Ting Lin, Shing‐Jyh Chang, Chia‐Hao Chan, Kevin W. Lyu, Jo‐Fan Chang, Eugenie Wong Soon May, Dai‐Ying Lin, Hsiu‐Chuan Chou, Hong‐Lin Chan Tags: Original Article Source Type: research

HSPA12B inhibits lipopolysaccharide‐induced inflammatory response in human umbilical vein endothelial cells
This study investigated the effects of HSPA12B on lipopolysaccharide (LPS)‐induced inflammatory responses in human umbilical vein endothelial cells (HUVECs) and the possible mechanisms involved. A HUVECs inflammatory model was induced by LPS. Overexpression of HSPA12B in HUVECs was achieved by infection with recombinant adenoviruses encoding green fluorescence protein‐HSPA12B. Knockdown of HSPA12B was achieved by siRNA technique. Twenty four hours after virus infection or siRNA transfection, HUVECs were stimulated with 1 μg/ml LPS for 4 hrs. Endothelial cell permeability ability was determined by transwell permeabil...
Source: Journal of Cellular and Molecular Medicine - December 1, 2014 Category: Molecular Biology Authors: Jun Wu, Xuehan Li, Lei Huang, Surong Jiang, Fei Tu, Xiaojin Zhang, He Ma, Rongrong Li, Chuanfu Li, Yuehua Li, Zhengnian Ding, Li Liu Tags: Original Article Source Type: research

Connexin 31.1 degradation requires the Clathrin‐mediated autophagy in NSCLC cell H1299
In this study, Cx31.1 was suggested to be degraded through both ubiquitin–proteasome system (UPS) and autophagy. Blockage of UPS with MG‐132 increased Cx31.1 level, but could not inhibit the degradation of Cx31.1 completely. In H1299 cells stably expressing Cx31.1, Cx31.1 reduced when autophagy was induced through starvation or Brefeldin A treatment. Knockdown of autophagy‐related protein ATG5 could increase the cellular level of Cx31.1 both under normal growth condition and starvation‐induced autophagy. Colocalization of Cx31.1 and autophagy marker light chain 3 (LC3) was revealed by immunofluorescence analysis. C...
Source: Journal of Cellular and Molecular Medicine - November 11, 2014 Category: Molecular Biology Authors: Xingli Zhu, Zhenchao Ruan, Xiufang Yang, Kaili Chu, Hai Wu, Yao Li, Yan Huang Tags: Original Article Source Type: research

HDAC‐inhibitor (S)‐8 disrupts HDAC6‐PP1 complex prompting A375 melanoma cell growth arrest and apoptosis
Abstract Histone deacetylase inhibitors (HDACi) are agents capable of inducing growth arrest and apoptosis in different tumour cell types. Previously, we reported a series of novel HDACi obtained by hybridizing SAHA or oxamflatin with 1,4‐benzodiazepines. Some of these hybrids proved effective against haematological and solid cancer cells and, above all, compound (S)‐8 has emerged for its activities in various biological systems. Here, we describe the effectiveness of (S)‐8 against highly metastatic human A375 melanoma cells by using normal PIG1 melanocytes as control. (S)‐8 prompted: acetylation of histones H3/H4 ...
Source: Journal of Cellular and Molecular Medicine - November 1, 2014 Category: Molecular Biology Authors: Manjola Balliu, Luca Guandalini, Maria Novella Romanelli, Massimo D'Amico, Francesco Paoletti Tags: Original Article Source Type: research

PDGF beta targeting in cervical cancer cells suggest a fine‐tuning of compensatory signalling pathways to sustain tumourigenic stimulation
In this study, we used siRNA against PDGF beta (PDGFBB) to investigate the cellular and molecular mechanisms of PDGFBB signalling in Ca Ski and HeLa cervical cancer cells. Our results show that PDGFBB inhibition in Ca Ski cells led to rapid alterations of the transcriptional pattern of 579 genes, genes that are known to have antagonistic roles in regulating tumour progression. Concomitantly, with the lack of significant effects on cervical cancer cells proliferation, apoptosis, migration or invasion, these findings suggests that cervical cancer cells shift between compensatory signalling pathways to maintain their behaviou...
Source: Journal of Cellular and Molecular Medicine - October 14, 2014 Category: Molecular Biology Authors: Oana Mihaela Tudoran, Olga Soritau, Loredana Balacescu, Laura Pop, Guillaume Meurice, Simona Visan, Staffan Lindberg, Alexandru Eniu, Ulo Langel, Ovidiu Balacescu, Ioana Berindan‐Neagoe Tags: Original Article Source Type: research