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Source: Molecular and Cellular Biochemistry

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Total 162 results found since Jan 2013.

Estrogen-independent role of ER α in ovarian cancer progression induced by leptin/Ob-Rb axis.
In conclusion, our findings demonstrated that ERα, but not ERβ, is involved in leptin-induced ovarian cancer in an E2-independent manner, providing new evidence for cancer progression in obesity-associated ovarian cancer. PMID: 31077012 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - May 9, 2019 Category: Biochemistry Authors: Ghasemi A, Saeidi J, Mohtashami M, Hashemy SI Tags: Mol Cell Biochem Source Type: research

Exosomes: cell-created drug delivery systems.
Abstract Exosomes are 40- to 100- nm cell-originated vesicles derived from endocytic compartments that are released into almost all biological fluids. Exosomes are cell-created vesicles that inherit identical phospholipid membrane, explaining a wide application of electroporation as a technique for exosomes loading with exogenous cargoes. Another way of loading exosomes with therapeutic cargo is to overexpress a certain gene in exosome-donor cells or treat cell line with drug of interest that later will be gently enveloped into vesicles based on the process of EV biogenesis. Similarly, to visualize siRNA loading i...
Source: Molecular and Cellular Biochemistry - May 8, 2019 Category: Biochemistry Authors: Familtseva A, Jeremic N, Tyagi SC Tags: Mol Cell Biochem Source Type: research

Activation of EMT in colorectal cancer by MTDH/NF- κB p65 pathway.
This study tries to investigate the effect of MTDH gene up-regulation on the activation of EMT in colorectal cancer (CRC) cells and identify the role of NF-κB p65. The CaCO2 cells were divided into three groups: one control group of cultured CaCO2 cells (C1), and two groups of CaCO2 cells co-transfected using human MTDH expression plasmid with either siRNA targeting human NF-κB p65 or its negative control (C2 and C3 respectively). The gene modification was confirmed by qPCR and the effect of gene modification on CRC aggravation was studied. MTDH up-regulation significantly promoted CRC cell proliferation, activated anaer...
Source: Molecular and Cellular Biochemistry - February 28, 2019 Category: Biochemistry Authors: El-Ashmawy NE, El-Zamarany EA, Khedr EG, Abo-Saif MA Tags: Mol Cell Biochem Source Type: research

Heat shock protein 60 negatively regulates the biological functions of ubiquitin-like protein MNSF β in macrophages.
In this study, we purified a 65 kDa MNSFβ adduct from mouse liver lysates by sequential chromatography on DEAE and glutathione S-transferase (GST)-fusioned MNSFβ immobilized on glutathione-Sepharose beads in the presence of ATP. MALDI-TOF mass spectrometry fingerprinting revealed that this MNSFβ adduct consists of an 8.5 kDa MNSFβ and heat shock protein 60 (HSP60), a mitochondrial protein involved in protein folding. Fingerprinting analysis of the MNSFβ adduct demonstrates that MNSFβ conjugates to HSP60 with a linkage between the C-terminal Gly74 and Lys481. HSP60 siRNA neutralized the inhibition of apoptosis by MN...
Source: Molecular and Cellular Biochemistry - February 1, 2019 Category: Biochemistry Authors: Nakamura M, Notsu K, Nakagawa M Tags: Mol Cell Biochem Source Type: research

Phosphorylation of TSC2 by PKC- δ reveals a novel signaling pathway that couples protein synthesis to mTORC1 activity.
Phosphorylation of TSC2 by PKC-δ reveals a novel signaling pathway that couples protein synthesis to mTORC1 activity. Mol Cell Biochem. 2019 Jan 25;: Authors: Zhan J, Chitta RK, Harwood FC, Grosveld GC Abstract Downstream of insulin-like growth factor receptor, the TSC1/2/ TCB1D7 (tuberous sclerosis complex) and mTOR (mechanistic target of rapamycin) pathways are implicated in many human diseases, including cancer and diabetes. Targeting this pathway is currently an important approach for palliating or eradicating cancer. Downstream of mTOR, translational machinery targeting holds great promise for a...
Source: Molecular and Cellular Biochemistry - January 25, 2019 Category: Biochemistry Authors: Zhan J, Chitta RK, Harwood FC, Grosveld GC Tags: Mol Cell Biochem Source Type: research

Up-regulation of PCSK9 gene expression and diminished level of LDL-receptor in rat liver as a potential cause of post-lipectomy hypercholesterolemia.
In conclusion, up-regulation of PCSK9, due to overexpression of HNF1α gene in liver of lipectomized rats and subsequently increase in circulating PCSK9 concentration lead to decrease in liver LDL-R level. This may contribute, at least in part, to an increase in the concentration of circulating cholesterol in rats with reduced fat mass. These findings provide a possible explanation for the molecular mechanism of hypercholesterolemia observed sometimes after reduction of fat mass in human. PMID: 30483910 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - November 27, 2018 Category: Biochemistry Authors: Dettlaff-Pokora A, Sucajtys-Szulc E, Sledzinski T Tags: Mol Cell Biochem Source Type: research

Dopamine promotes cathepsin B-mediated amyloid precursor protein degradation by reactive oxygen species-sensitive mechanism in neuronal cell.
Abstract Recent literature suggested an important function of native amyloid precursor protein (APP) as amine oxidase implicating in protection of brain cells from catecholamine-induced toxicity. However, any role of catecholamines on regulation of native APP has not been explored. Here we report that dopamine (DA), one of the most prominent catecholamine neurotransmitters in brain, down-modulates native APP protein in several neuronal cell types. Using SH-SY5Y cells as model, we detected no alteration of transcript expression and unaffected translation suggested that DA might induce APP degradation. We actually f...
Source: Molecular and Cellular Biochemistry - October 22, 2018 Category: Biochemistry Authors: Kumari S, Mukherjee A, Mukhopadhyay CK Tags: Mol Cell Biochem Source Type: research

HGF promotes HTR-8/SVneo cell migration through activation of MAPK/PKA signaling leading to up-regulation of WNT ligands and integrins that target β-catenin.
Abstract Inadequate migration and invasion of the trophoblast cells during embryo implantation is one of the reasons for pregnancy-related complications such as intrauterine growth restriction and preeclampsia. In the present study, relevance of WNT ligands and integrins associated with hepatocyte growth factor (HGF)-mediated migration of HTR-8/SVneo trophoblastic cells has been investigated. Treatment of HTR-8/SVneo cells with HGF led to a dose-dependent increase in their migration. RT-PCR studies revealed a significant increase in the transcripts of WNT4, WNT11, ITGA2, and ITGAV, which was further confirmed at p...
Source: Molecular and Cellular Biochemistry - August 22, 2018 Category: Biochemistry Authors: Chaudhary P, Malhotra SS, Babu GS, Sobti RC, Gupta SK Tags: Mol Cell Biochem Source Type: research

The utrophin-beta 2 syntrophin complex regulates adipocyte lipid droplet size independent of adipogenesis.
Abstract Utrophin is a widely expressed cytoskeleton protein and is associated with lipid droplets (LDs) in adipocytes. The scaffold protein beta 2 syntrophin (SNTB2) controls signaling events by recruiting distinct membrane and cytoskeletal proteins, and binds to utrophin. Here we show that SNTB2 forms a complex with utrophin in adipocytes. SNTB2 protein is strongly diminished when utrophin is low. Of note, knock-down of utrophin or SNTB2 enhances LD growth during adipogenesis. SNTB2 reduction has no effect on basal and induced lipolysis, and insulin-stimulated phosphorylation of Akt is normal. The antilipolytic ...
Source: Molecular and Cellular Biochemistry - July 16, 2018 Category: Biochemistry Authors: Krautbauer S, Neumeier M, Haberl EM, Pohl R, Feder S, Eisinger K, Rein-Fischboeck L, Buechler C Tags: Mol Cell Biochem Source Type: research

Loss of the basic helix-loop-helix transcription factor Bhlhe41 induces cell death and impairs neurite outgrowth in Neuro2a cells.
Abstract The basic helix-loop-helix (bHLH) superfamily of transcription factors have been implicated in a wide range of cellular functions such as proliferation, differentiation, tumorigenesis, and circadian rhythms. In a previous siRNA-based screen, bHLH family member e41 (BHLHE41) had been identified as a putative regulator of neuronal differentiation; however, its function remains largely elusive. To this end, using the CRISPR-Cas9 system, we established an isogenic Neuro2a (N2a) cell line with biallelic targeting of Bhlhe41 gene (Bhlhe41-/-). In undifferentiated N2a cells, complete knockout of Bhlhe41 resulted...
Source: Molecular and Cellular Biochemistry - June 20, 2018 Category: Biochemistry Authors: Sun Y, Zhang H, Wang L, Li J, Jin H, Wang Z, Tian S, Qi L, Liu X Tags: Mol Cell Biochem Source Type: research

Correlation between S100A11 and the TGF- β1/SMAD4 pathway and its effects on the proliferation and apoptosis of pancreatic cancer cell line PANC-1.
Correlation between S100A11 and the TGF-β1/SMAD4 pathway and its effects on the proliferation and apoptosis of pancreatic cancer cell line PANC-1. Mol Cell Biochem. 2018 Jun 19;: Authors: Ji YF, Li T, Jiang F, Ni WK, Guan CQ, Liu ZX, Lu CH, Ni RZ, Wu W, Xiao MB Abstract S100A11 as a S100 protein family member has been documented to play dual-direction regulation over cancer cell proliferation. We explored the role of S100A11 in the proliferation and apoptosis of pancreatic cancer cell line PANC-1 and the potential mechanisms involving the TGF-β1/SMAD4/p21 pathway. S100A11 and TGF-β1 protein express...
Source: Molecular and Cellular Biochemistry - June 19, 2018 Category: Biochemistry Authors: Ji YF, Li T, Jiang F, Ni WK, Guan CQ, Liu ZX, Lu CH, Ni RZ, Wu W, Xiao MB Tags: Mol Cell Biochem Source Type: research

Negative regulation of MAVS-mediated innate immune response by ASC.
Abstract Stringent control of the type I interferon signaling pathways is critical to effective host immune responses, however, the molecular mechanisms that negatively regulate these pathways are still poorly understood. Here, we show that apoptosis speck-like protein (ASC), an adaptor protein of inflammasome complex, can inhibit IFN-β signaling response by interacting with mitochondrial antiviral signaling protein (MAVS). Importantly, ASC-specific siRNA knockdown enhanced virus-induced type I interferon production, with consequent reduction of virus replication. Taken together, these results suggest that ASC, a...
Source: Molecular and Cellular Biochemistry - December 26, 2017 Category: Biochemistry Authors: Han Y, He X, Wei C, Song T, Zou L, Li Z, Ye J, Qi L, Li L, Zhong H, Wu F Tags: Mol Cell Biochem Source Type: research

Comprehensive bioinformation analysis of the miRNA of PLCE1 knockdown in esophageal squamous cell carcinoma.
In this study, PLCE1 siRNA and vector-only plasmid were stably transfected into Eca109 and EC9706 cells and then subjected to miRNA array analysis, and quantitative real-time PCR was applied to validate miRNA array data. Then bioinformatic analyses, such as GO and pathway software, were conducted to obtain data on these differentially expressed miRNAs-targeted genes (DEGs) and clarify their function and pathway. The results showed that 36 miRNAs were found to be differentially expressed in PLCE1 siRNA-transfected cells compared with the control cells. In particular, 28 miRNAs were upregulated while 8 miRNAs were downregula...
Source: Molecular and Cellular Biochemistry - December 13, 2017 Category: Biochemistry Authors: Cui X, Wang K, Yang X, Peng H, Chen X, Xin H, Tian Y, Chen Y, Li F Tags: Mol Cell Biochem Source Type: research

Lipopolysaccharide stimulates endogenous β-glucuronidase via PKC/NF-κB/c-myc signaling cascade: a possible factor in hepatolithiasis formation.
In this study, the mechanism of increased expression and secretion of endogenous β-glucuronidase during hepatolithiasis formation was investigated. We assessed the endogenous β-glucuronidase, c-myc, p-p65, and p-PKC expression in liver specimens with hepatolithiasis by immunohistochemical staining, and found that compared with that in normal liver samples, the expression of endogenous β-glucuronidase, c-myc, p-p65, and p-PKC in liver specimens with hepatolithiasis significantly increased, and their expressions were positively correlated with each other. Lipopolysaccharide (LPS) induced increased expression of endogenous...
Source: Molecular and Cellular Biochemistry - November 29, 2017 Category: Biochemistry Authors: Yao D, Dong Q, Tian Y, Dai C, Wu S Tags: Mol Cell Biochem Source Type: research

EZH2 promotes cell proliferation by regulating the expression of RUNX3 in laryngeal carcinoma.
Abstract Enhancer of zeste homolog 2 (EZH2) is a highly conserved histone methyltransferase, which is overexpressed in different types of cancers such as breast and prostate cancer. It is reported that EZH2 can directly down-regulate RUNX3 by increasing histone H3 methylation. However, the role of EZH2 in the development and progression of laryngeal carcinoma has not yet been investigated, and the relationship between EZH2 and RUNX3 in laryngeal carcinoma is rarely reported. The current study aims to determine the role of EZH2 in the progression of laryngeal carcinoma, and investigate the interaction between EZH2 ...
Source: Molecular and Cellular Biochemistry - August 9, 2017 Category: Biochemistry Authors: Lian R, Ma H, Wu Z, Zhang G, Jiao L, Miao W, Jin Q, Li R, Chen P, Shi H, Yu W Tags: Mol Cell Biochem Source Type: research