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Source: Molecular and Cellular Biochemistry
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Total 162 results found since Jan 2013.
Dyslipidemia regulates thrombospondin-1-induced vascular smooth muscle cell chemotaxis.
In conclusion, HDL/S1P inhibits, while LDL/LPA stimulates TSP-1-induced VSMC chemotaxis. The HDL/S1P effect is mediated by the S1PR1-3 receptors. The LDL/LPA effects are mediated by the LPAR1 and LPAR2 receptors, but not LPAR3. Therefore, lipids have significant effects on TSP-1-induced VSMC chemotaxis.
PMID: 26350564 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - September 8, 2015 Category: Biochemistry Authors: Desai P, Stein JJ, Siddiqui SA, Maier KG, Gahtan V Tags: Mol Cell Biochem Source Type: research
Irradiation inhibits the maturation and mineralization of osteoblasts via the activation of Nrf2/HO-1 pathway.
Abstract
Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) regulates the induction of antioxidant gene expression and protects cells against oxidative injury. However, there are controversial findings regarding the roles of Nrf2 on bone metabolism under oxidative stress. The role of Nrf2 on the differentiation of radiation-exposed osteoblasts is also unclear. We investigated whether Nrf2 negatively or positively affects osteoblast differentiation in response to irradiation. Irradiation inhibited osteoblast differentiation of MC3T3-E1 cells in a dose-dependent manner. This inhibition was evidenced by the irradiati...
Source: Molecular and Cellular Biochemistry - September 7, 2015 Category: Biochemistry Authors: Kook SH, Kim KA, Ji H, Lee D, Lee JC Tags: Mol Cell Biochem Source Type: research
Gensenoside Rb1 protects rat PC12 cells from oxidative stress-induced endoplasmic reticulum stress: the involvement of thioredoxin-1.
Abstract
Oxidative stress plays an important role in many diseases and hydrogen peroxide (H2O2) plays a central role in the stress. Gensenoside Rb1 is the one of active ingredients in the traditional Chinese medicine Panax notoginseng. It has been reported that gensenoside Rb1 possesses various pharmacological activities. Here we report that gensenoside Rb1 exhibits potent protective effects against oxidative injury induced by H2O2 through inhibiting endoplasmic reticulum stress in PC12 cells. Cell viability assay demonstrated that incubation with H2O2 for 24 h led to a significant loss of cultured rat PC12 c...
Source: Molecular and Cellular Biochemistry - September 7, 2015 Category: Biochemistry Authors: Zeng XS, Jia JJ, Ma LF Tags: Mol Cell Biochem Source Type: research
NF45 overexpression is associated with poor prognosis and enhanced cell proliferation of pancreatic ductal adenocarcinoma.
In this study, we investigated the clinical relevance and potential role of NF45 expression in PDAC development. Western blot analysis revealed that NF45 was remarkably upregulated in PDAC tissues, compared with the adjacent non-tumorous ones. In addition, the expression of NF45 in 122 patients with PDAC was evaluated using immunohistochemistry. In this way, we found that NF45 was abundantly expressed in PDAC tissues, and the expression of NF45 was correlated with tumor size (p = 0.007), histological differentiation (p = 0.033), and TNM stage (p = 0.001). Importantly, patients with low levels of NF45 expression exhib...
Source: Molecular and Cellular Biochemistry - August 15, 2015 Category: Biochemistry Authors: Wan C, Gong C, Ji L, Liu X, Wang Y, Wang L, Shao M, Yang L, Fan S, Xiao Y, Wang X, Li M, Zhou G, Zhang Y Tags: Mol Cell Biochem Source Type: research
miR-25 promotes glioblastoma cell proliferation and invasion by directly targeting NEFL.
In this study, we demonstrated that miR-25 was significantly up-regulated in astrocytoma tissues and glioblastoma cell lines. In vitro studies further demonstrated that overexpressed miR-25 was able to promote, while its antisense oligos inhibited cell proliferation and invasion in U251 cells. Moreover, we identified neurofilament light polypeptide (NEFL) as a novel target molecule of miR-25. Also of note was the fact that NEFL was down-regulated with increased levels of miR-25 expression in human astrocytoma clinical specimens. In addition, via the mTOR signaling pathway, NEFL-siRNA could significantly attenuate the inhib...
Source: Molecular and Cellular Biochemistry - July 26, 2015 Category: Biochemistry Authors: Peng G, Yuan X, Yuan J, Liu Q, Dai M, Shen C, Ma J, Liao Y, Jiang W Tags: Mol Cell Biochem Source Type: research
Edaravone protects osteoblastic cells from dexamethasone through inhibiting oxidative stress and mPTP opening.
Abstract
Existing evidences have emphasized an important role of oxidative stress in dexamethasone (Dex)-induced osteoblastic cell damages. Here, we investigated the possible anti-Dex activity of edaravone in osteoblastic cells, and studied the underlying mechanisms. We showed that edaravone dose-dependently attenuated Dex-induced death and apoptosis of established human or murine osteoblastic cells. Further, Dex-mediated damages to primary murine osteoblasts were also alleviated by edaravone. In osteoblastic cells/osteoblasts, Dex induced significant oxidative stresses, tested by increased levels of reactive oxyg...
Source: Molecular and Cellular Biochemistry - July 16, 2015 Category: Biochemistry Authors: Sun WX, Zheng HY, Lan J Tags: Mol Cell Biochem Source Type: research
SRD5A2 gene expression inhibits cell migration and invasion in prostate cancer cell line via F-actin reorganization.
Abstract
Steroid 5-alpha reductase type 2 (SRD5A2) gene is important for normal development and functioning of prostate gland but it is reported to be silenced in metastatic prostate cancer. We showed that exogenous SRD5A2 expression in prostate cancer cell line reduced cell migration and invasion. Additionally, the stable transfectants showed enhanced adhesion to the matrix accompanied by changes in cytoskeletal organization, involving actin polymerization. siRNA knockdown of the endogenous SRD5A2 mRNA in LnCAP cells was effective, it reversed the phenotype, and thus induced cell motility. The MEK1 and pERK1/2 le...
Source: Molecular and Cellular Biochemistry - June 20, 2015 Category: Biochemistry Authors: Aggarwal S, Singh M, Kumar A, Mukhopadhyay T Tags: Mol Cell Biochem Source Type: research
ATF6 pathway of unfolded protein response mediates advanced oxidation protein product-induced hypertrophy and epithelial-to-mesenchymal transition in HK-2 cells.
In this study, we investigated the roles of the protein kinase RNA-like ER kinase (PERK), activating transcription factor 6 (ATF6), and inositol-requiring enzyme 1 (IRE1) pathways of UPR in this process in HK-2 cells. AOPP treatment induced the overexpression of cleaved ATF6 and spliced form of X-box binding protein-1, and induced the phosphorylation of PERK, eukaryotic translation initiation factor 2α and IRE1. Furthermore, silencing of ATF6 increased E-cadherin and zonula occludens-1 expression, lowered the expression of vimentin, and downregulated total protein content, whereas knockdown of PERK or IRE1 resulted in no ...
Source: Molecular and Cellular Biochemistry - June 5, 2015 Category: Biochemistry Authors: Tang X, Liang X, Li M, Guo T, Duan N, Wang Y, Rong G, Yang L, Zhang S, Zhang J Tags: Mol Cell Biochem Source Type: research
AP4 activates cell migration and EMT mediated by p53 in MDA-MB-231 breast carcinoma cells.
Abstract
Tumor metastasis is the primary cause of mortality in most cancer patients. Before disassociation from the tumors, most of malignant tumor cells undergo the epithelial-mesenchymal transition to break away from the adhesions between the cells and the surrounding extracellular matrix. Recently, activating enhancer-binding protein (AP4) has been shown to be a mediator of EMT in colorectal cancer and high level of AP4 correlates with poor prognosis in cancer patients. It has been found that AP4 upregulates the genes involved in EMT and cell proliferation in colorectal cancer cells and that the aggressive hum...
Source: Molecular and Cellular Biochemistry - June 3, 2015 Category: Biochemistry Authors: Chen S, Chiu SK Tags: Mol Cell Biochem Source Type: research
ERK5 signalling pathway is essential for fluid shear stress-induced COX-2 gene expression in MC3T3-E1 osteoblast.
Abstract
Bone cells respond to various mechanical stimuli including fluid shear stress (FSS) in vitro. Induction of cyclooxygenase-2 (COX-2) is thought to be important for the anabolic effects of mechanical loading. Recently, extracellular-signal-regulated kinase 5 (ERK5) has been found to be involved in multiple cellular processes. However, the relationship between ERK5 and the induction of COX-2 is still unknown. Here, we investigated the potential involvement of ERK5 in the response of pre-osteoblastic MC3T3-E1 cells upon FSS. MC3T3-E1 cells were subjected to 12 dyn/cm(2) FSS. Then, we established a ERK5 small ...
Source: Molecular and Cellular Biochemistry - May 15, 2015 Category: Biochemistry Authors: Jiang J, Zhao LG, Teng YJ, Chen SL, An LP, Ma JL, Wang J, Xia YY Tags: Mol Cell Biochem Source Type: research
HDAC7 modulates TNF-α-mediated suppression of Leydig cell steroidogenesis.
Abstract
The pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-α) has an inhibitory role in gonadal functions particularly in the steroidogenesis of Leydig cells. In the present study, we demonstrate that TNF-α activates histone deacetylases 7 (HDAC7), which regulates the expression of steroidogenic enzyme genes in Leydig cells. LC-540 Leydig cells were treated with TNF-α (10 ng/ml) for different time intervals. TNF-α treatment significantly suppressed histone H3 acetylation and methylation and, concomitantly, increased the total histone deacetylases activity in LC-540 Leydig cells. RT-PCR and wester...
Source: Molecular and Cellular Biochemistry - April 28, 2015 Category: Biochemistry Authors: Sadasivam M, Ramatchandirin B, Balakrishnan S, Prahalathan C Tags: Mol Cell Biochem Source Type: research
MiR-21 overexpression improves osteoporosis by targeting RECK.
Abstract
Osteoporosis is a kind of metabolic bone disorder. MicroRNA-21 (miR-21) has been proven to play an important role in bone formation, whereas its role in osteoporosis is unclear. In the present study, miR-21 expression was inhibited by TNF-α in mesenchymal stem cells (MSCs). TNF-α induced cell apoptosis, and inhibited cell proliferation and differentiation of MSCs. Whereas the effect was reversed by miR-21 mimics. Expression of reversion-inducing cysteine-rich protein with Kazal motifs (RECK) which is a predicted target of miR-21 was inhibited by miR-21 mimics. A luciferase reporter gene assay showed th...
Source: Molecular and Cellular Biochemistry - April 17, 2015 Category: Biochemistry Authors: Zhao W, Dong Y, Wu C, Ma Y, Jin Y, Ji Y Tags: Mol Cell Biochem Source Type: research
Clopidogrel reduces apoptosis and promotes proliferation of human vascular endothelial cells induced by palmitic acid via suppression of the long non-coding RNA HIF1A-AS1 in vitro.
This study investigated the effects of clopidogrel on palmitic acid-induced damage of human vascular endothelial cells (HUVECs), and the molecular mechanisms of LncRNA HIF1A-AS1 in regulating the proliferation and apoptosis of HUVECs in vitro. We firstly established a damage model of HUVECs through palmitic acid (PA) treatment. And the effect of clopidogrel reducing PA-induced apoptosis of HUVECs was observed by the flow cytometric measurement. To further understand the molecular mechanism of clopidogrel rescues PA-induced apoptosis, we used human LncRNA PCR array to compare the LncRNA expression profile difference between...
Source: Molecular and Cellular Biochemistry - March 12, 2015 Category: Biochemistry Authors: Wang J, Chen L, Li H, Yang J, Gong Z, Wang B, Zhao X Tags: Mol Cell Biochem Source Type: research
Inhibiting (pro)renin receptor-mediated p38 MAPK signaling decreases hypoxia/reoxygenation-induced apoptosis in H9c2 cells.
In this study, we hypothesized that p38 mitogen-activated protein kinase (MAPK) signaling pathway activation by the (pro)renin receptor had effects on myocardial apoptosis induced by ischemia/reperfusion. This analysis was performed using a hypoxia/reoxygenation model in H9c2 cells to mimic ischemia/reperfusion injury. The H9c2 rat cardiomyocyte cell line was subjected to 2 h of hypoxia followed by 6 h of reoxygenation. The (pro)renin receptor, caspase 3, and phosphorylated p38 MAPK protein expression levels were analyzed by Western blot. After 2 h of hypoxia followed by 6 h of reoxygenation, apoptosis increased in H9c...
Source: Molecular and Cellular Biochemistry - February 25, 2015 Category: Biochemistry Authors: Liu Y, Zhang S, Su D, Liu J, Cheng Y, Zou L, Li W, Jiang Y Tags: Mol Cell Biochem Source Type: research
Improved survival of mesenchymal stem cells by macrophage migration inhibitory factor.
We examined the protective effect of MIF on hypoxia/serum deprivation (SD)-induced apoptosis of mesenchymal stem cells (MSCs), as well as the possible mechanisms. MSCs were obtained from rat bone marrow and cultured in vitro. Apoptosis was induced by culturing MSCs under hypoxia/SD conditions for up to 24 h and assessed by flow cytometry. Expression levels of c-Met, Akt, and FOXO3a were detected by Western blotting. CD74 expression was detected by qRT-PCR, Western blot, and immunofluorescence. Oxidative stress under hypoxia/SD was examined by detection of reactive oxygen species (ROS) and activity of superoxide dismutase ...
Source: Molecular and Cellular Biochemistry - February 21, 2015 Category: Biochemistry Authors: Xia W, Xie C, Jiang M, Hou M Tags: Mol Cell Biochem Source Type: research
