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Source: Molecular and Cellular Biochemistry

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Total 162 results found since Jan 2013.

The siRNA cocktail targeting VEGF and HER2 inhibition on the proliferation and induced apoptosis of gastric cancer cell.
Abstract The aim of this study was to investigate the inhibitory effect of a siRNA cocktail targeting Vascular endothelial growth factor (VEGF) and Human epidermal growth factor receptor 2 (HER2) on cell proliferation, induced apoptosis and the expression of VEGF and HER2 in human gastric carcinoma cell. The silencing rate of pre-designed siRNAs that targeted VEGF and HER2 was detected by Real-time Quantitative PCR (RT-QPCR) analysis. Furthermore, the best silencing siRNA that targeted VEGF and HER2 was prepared as a cocktail to co-knockdown VEGF and HER2 expression at both mRNA and protein levels which were detec...
Source: Molecular and Cellular Biochemistry - October 26, 2013 Category: Biochemistry Authors: Liu K, Chen H, You Q, Shi H, Wang Z Tags: Mol Cell Biochem Source Type: research

Effects of sialidase NEU1 siRNA on proliferation, apoptosis, and invasion in human ovarian cancer.
In conclusion, NEU1 siRNA can effectively inhibit proliferation, apoptosis, and invasion of human ovarian cancer cells by targeting lysosome and oxidative phosphorylation signaling, which can serve as a new target ovarian cancer treatment. PMID: 26463994 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - October 13, 2015 Category: Biochemistry Authors: Ren LR, Zhang LP, Huang SY, Zhu YF, Li WJ, Fang SY, Shen L, Gao YL Tags: Mol Cell Biochem Source Type: research

Connexin43 siRNA promotes HUVEC proliferation and inhibits apoptosis induced by ox-LDL: an involvement of ERK signaling pathway.
In this study, we investigated the effects of ox-LDL on connexin43 and the mechanisms of connexin43 siRNA-inhibited apoptosis induced by ox-LDL in human umbilical vein endothelial cell (HUVEC), to clarify the role of connexin43 in atherosclerosis. Our results showed that ox-LDL significantly inhibited the growth and promoted apoptosis of HUVEC in a dose-dependent manner. Also, ox-LDL upregulated the expression of connexin43. Furthermore, knockdown connexin43 by siRNA promoted proliferation and inhibited apoptosis in ox-LDL-stimulated HUVEC. Moreover, the level of phosphor-ERK1/2 and connexin43 was remarkably attenuated by ...
Source: Molecular and Cellular Biochemistry - May 16, 2014 Category: Biochemistry Authors: Yin G, Yang X, Li B, Yang M, Ren M Tags: Mol Cell Biochem Source Type: research

The inhibitory effect of a new scFv/tP protein as siRNA delivery system to target hWAPL in cervical carcinoma.
Abstract Targeted immunotherapy has become a popular research topic in cancer. The development and metastasis of cervical carcinoma are closely related to epidermal growth factor (EGF) and EGF-1 receptor (EGFR). We successfully constructed a single-chain human anti-EGFR antibody (scFv) and truncated protamine (tP) fusion protein (scFV/tP) expression vector using overlap extension PCR. Enzyme-linked immunosorbent assay and gel shift assay showed that the fusion protein retained the DNA and antigen-binding activity of the original antibody. Using the non-viral scFv/tP vector as a delivery tool, small interfering RNA...
Source: Molecular and Cellular Biochemistry - February 25, 2014 Category: Biochemistry Authors: Zhang H, Mao Y, Zhang F, Ye C, Tong H, Su Y, Zhu J Tags: Mol Cell Biochem Source Type: research

Unrevealing the role of P-protein on melanosome biology and structure, using siRNA-mediated down regulation of OCA2.
Abstract The pink-eyed dilution protein (P-protein) plays a critical role in melanin synthesis in melanocytes and retinal pigment epithelium cells. Mutation in this protein may cause complete or partial albinism. Role of the P-protein ranges in melanin synthesis to maturation and trafficking of the melanosomes. The aim of the present study was to evaluate the effect of P-protein inhibition on melanosome biology by comparing the shape, size, count, and types of melanosomes in melan-a melanocytes. The cells were extensively examined by the transmission electron microscopy. The P-protein inhibition was carried by P-p...
Source: Molecular and Cellular Biochemistry - February 6, 2015 Category: Biochemistry Authors: Park S, Morya VK, Nguyen DH, Singh BK, Lee HB, Kim EK Tags: Mol Cell Biochem Source Type: research

Expression of HAb18G in non-small lung cancer and characterization of activation, migration, proliferation, and apoptosis in A549 cells following siRNA-induced downregulation of HAb18G.
Abstract HAb18G, a novel cancer biomarker, has been shown to be involved in the progression of malignancy by regulating expression of vascular endothelial growth factor (VEGF) and matrixmetalloproteinases (MMPs). The goal of this study was to evaluate the role of HAb18G in the biology of NSCLC and to determine its potential as a therapeutic target. HAb18G protein expression was detected by immunohistochemistry in 150 NSCLC tissues. The results showed that HAb18G protein expression was associated with tumor diameter, lymph node status, tumor stage, and poor prognosis (P < 0.05). Multivariate analysis showed th...
Source: Molecular and Cellular Biochemistry - September 8, 2013 Category: Biochemistry Authors: Xu X, Liu S, Lei B, Li W, Lin N, Sheng W, Huang A, Shen H Tags: Mol Cell Biochem Source Type: research

The siRNA-mediated downregulation of N-Ras sensitizes human melanoma cells to apoptosis induced by selective BRAF inhibitors.
Abstract The clinical benefit of selective BRAF inhibitor therapies is limited by the emergence of drug resistance. Here, we investigated the molecular basis underlying the acquired resistance to a BRAF inhibitor by comparing the signaling pathways in the parental A375P cells and the resistant subline (A375P/Mdr). We demonstrate that MAPK re-activation does not contribute to the mechanism of resistance to UAI-201 of A375P/Mdr cells. The relative quantitative analysis using the 2(-ΔΔCt) method revealed that the BRAF inhibitor resistance observed in A375P/Mdr cells is not mediated through the overexpression of MDR...
Source: Molecular and Cellular Biochemistry - March 27, 2014 Category: Biochemistry Authors: Ahn JH, Lee M Tags: Mol Cell Biochem Source Type: research

Knockdown of cardiac Kir3.1 gene with siRNA can improve bradycardia in an experimental sinus bradycardia rat model.
The objective of this study was to explore whether the inhibition of potassium inwardly rectifying channel (Kir3.1) with short interfering RNA (siRNA) can improve bradycardia in an experimental sinus bradycardia rat model. 54 Sprague Dawley (SD) rats were randomly divided into three groups: experimental group, control group, and sham group. Sinus bradycardia model was established in SD rats through chemical ablation of sinoatrial (SA) node with 20% formaldehyde. Variations of Kir3.1 expression at mRNA and protein level were examined with qPCR and Western blotting. Electrocardiograms (ECG) of rats at 3 days and 1, 2, 3, an...
Source: Molecular and Cellular Biochemistry - February 14, 2017 Category: Biochemistry Authors: Li Y, Fu X, Zhang Z, Yu B Tags: Mol Cell Biochem Source Type: research

New perspectives in triple-negative breast cancer therapy based on treatments with TGF β1 siRNA and doxorubicin.
In this study, a combined doxorubicin (DOX) and small interfering RNA (siRNA) therapy is proposed as therapeutic strategy for targeting TGFβ1 gene. Hs578T cell line is used as in vitro model for TNBC, wherein TGFβ1siRNA therapy is employed to enhance therapeutic effects. Cell proliferation rate is measured using an MTT test, and morphological alterations are assed using microscopically approached, while gene expression is determined by qRT-PCR analysis. The combined treatment of TGFβ1siRNA and DOX reduced levels of cell proliferation and mitochondrial activity and promoted the alteration of cell morphology (dark-field m...
Source: Molecular and Cellular Biochemistry - September 3, 2020 Category: Biochemistry Authors: Ciocan-Cȃrtiţă CA, Jurj A, Raduly L, Cojocneanu R, Moldovan A, Pileczki V, Pop LA, Budişan L, Braicu C, Korban SS, Berindan-Neagoe I Tags: Mol Cell Biochem Source Type: research

EZH2 promotes cell proliferation by regulating the expression of RUNX3 in laryngeal carcinoma.
Abstract Enhancer of zeste homolog 2 (EZH2) is a highly conserved histone methyltransferase, which is overexpressed in different types of cancers such as breast and prostate cancer. It is reported that EZH2 can directly down-regulate RUNX3 by increasing histone H3 methylation. However, the role of EZH2 in the development and progression of laryngeal carcinoma has not yet been investigated, and the relationship between EZH2 and RUNX3 in laryngeal carcinoma is rarely reported. The current study aims to determine the role of EZH2 in the progression of laryngeal carcinoma, and investigate the interaction between EZH2 ...
Source: Molecular and Cellular Biochemistry - August 9, 2017 Category: Biochemistry Authors: Lian R, Ma H, Wu Z, Zhang G, Jiao L, Miao W, Jin Q, Li R, Chen P, Shi H, Yu W Tags: Mol Cell Biochem Source Type: research

Effects of miR-202-5p silencing PIK3CA gene expression on proliferation, invasion, and epithelial-mesenchymal transition of cervical cancer SiHa cells through inhibiting PI3K/Akt/mTOR signaling pathway activation
In conclusion, the overexpression of miR-202-5p can suppress PIK3CA gene expression and the activation of PI3K/Akt/mTOR signaling pathway to suppress the proliferation, invasion, and EMT of cervical cancer.PMID:34244973 | DOI:10.1007/s11010-021-04211-4
Source: Molecular and Cellular Biochemistry - July 10, 2021 Category: Biochemistry Authors: Yan Zheng Lei Xie Shuwen Xu Weidong Yan Hongzhen Zhang Yali Meng Jingqiao Liu Xujing Wei Source Type: research

Role of hepcidin and its downstream proteins in early brain injury after experimental subarachnoid hemorrhage in rats.
In conclusion, downregulation of ferroportin-1 and ceruloplasmin caused by hepcidin enhanced iron-dependent oxidative damage and may be the potential mechanism of SAH. PMID: 27250827 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - May 31, 2016 Category: Biochemistry Authors: Tan G, Liu L, He Z, Sun J, Xing W, Sun X Tags: Mol Cell Biochem Source Type: research