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Source: Molecular and Cellular Biochemistry

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Total 162 results found since Jan 2013.

Knockdown BMI1 expression inhibits proliferation and invasion in human bladder cancer T24 cells.
Abstract B cell-specific moloney murine leukemia virus integration site 1 (BMI1) is a transcriptional repressor of polycomb repressive complex 1, which is involved in the proliferation, senescence, migration, and tumorigenesis of cancer. Experimental researchers have convincingly linked BMI1 to tumorigenesis. However, there is no study about the issue on the role of BMI1 in the proliferation, apoptosis, and migration of bladder cancer. To address this question, we examined the expression of BMI1 in bladder cancer tissues and used siRNA to knockdown BMI1 expression in bladder cancer T24 cells. Then we tested the ce...
Source: Molecular and Cellular Biochemistry - July 3, 2013 Category: Biochemistry Authors: Liang W, Zhu D, Cui X, Su J, Liu H, Han J, Zhao F, Xie W Tags: Mol Cell Biochem Source Type: research

p53siRNA therapy reduces cell proliferation, migration and induces apoptosis in triple negative breast cancer cells.
This study is intended to investigate, the potential applications of RNA interference (RNAi) to block p53 expression, as well as its subsequent effect on cell growth, apoptosis and migration on a triple negative human breast cancer cell line (Hs578T). p53siRNA significantly reduced cell index (CI) compared to the control and we observed an inhibition of cellular migration in the interval of time between 0 and 30 h, as shown in the data obtained by dynamic evaluation using the xCELLigence System. Also, by using PCR-array technology, a panel of 84 key genes involved in apoptosis was investigated. Our studies indicate that t...
Source: Molecular and Cellular Biochemistry - July 27, 2013 Category: Biochemistry Authors: Braicu C, Pileczki V, Irimie A, Berindan-Neagoe I Tags: Mol Cell Biochem Source Type: research

The role of HMGB1-RAGE axis in migration and invasion of hepatocellular carcinoma cell lines.
Abstract High mobility group protein box1 (HMGB1) and its receptor-receptor for advanced glycation end products (RAGE) are pivotal factors in the development and progression of many types of tumor, but the role of HMGB1-RAGE axis in hepatocellular carcinoma (HCC) especially its effects on metastasis and recurrence remains obscure. Here, we report the role of HMGB1-RAGE axis in the biological behaviors of HCC cell lines and the underlying molecular mechanism. We show that the expressions of HMGB1, RAGE, and extracellular HMGB1 increase consistently according to cell metastasis potentials, while the concentration of...
Source: Molecular and Cellular Biochemistry - February 9, 2014 Category: Biochemistry Authors: Chen RC, Yi PP, Zhou RR, Xiao MF, Huang ZB, Tang DL, Huang Y, Fan XG Tags: Mol Cell Biochem Source Type: research

The expression and functional role of a FOXC1 related mRNA-lncRNA pair in oral squamous cell carcinoma.
In conclusion, FOXC1 may be co-amplified with FOXCUT in OSCC, and both of them may be functionally involved in the tumor progression of OSCC. This provides evidence that both FOXC1 and FOXCUT may serve as novel biomarkers and therapeutic targets in OSCC patients who overexpress this "lncRNA-mRNA pair". PMID: 24889262 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - June 3, 2014 Category: Biochemistry Authors: Kong XP, Yao J, Luo W, Feng FK, Ma JT, Ren YP, Wang DL, Bu RF Tags: Mol Cell Biochem Source Type: research

P53 induction accompanying G2/M arrest upon knockdown of tumor suppressor HIC1 in U87MG glioma cells.
In this study, we investigated the role of HIC1 in cell cycle and proliferation of glioma cell line U87MG which has wild type p53, in both serum-containing and serum-deprived medium. Microscopic analysis and MTT assay showed reduced cell number and rate of proliferation upon HIC1 knock down compared to control siRNA (p = 0.025) and untreated cells (p = 0.03) in serum-containing medium and serum-free medium (p = 0.014 vs control siRNA; p = 0.018 vs untreated cells). Cell cycle analysis revealed an arrest at G2/M phase of cell cycle with no demonstrable increase in apoptosis with both medium. An increased expression ...
Source: Molecular and Cellular Biochemistry - July 4, 2014 Category: Biochemistry Authors: Kumar S Tags: Mol Cell Biochem Source Type: research

MCPIP1 contributes to the toxicity of proteasome inhibitor MG-132 in HeLa cells by the inhibition of NF-κB.
Abstract Recently, we have shown that the treatment of cells with proteasome inhibitor MG-132 results in the induction of expression of monocyte chemotactic protein-1 induced protein 1 (MCPIP1). MCPIP1 is a ribonuclease, responsible for the degradation of transcripts encoding certain pro-inflammatory cytokines. The protein is also known as an inhibitor of NF-κB transcription factor. Thanks to its molecular properties, MCPIP1 is considered as a regulator of inflammation, differentiation, and survival. Using siRNA technology, we show here that MCPIP1 expression contributes to the toxic properties of MG-132 in HeLa ...
Source: Molecular and Cellular Biochemistry - July 4, 2014 Category: Biochemistry Authors: Skalniak L, Dziendziel M, Jura J Tags: Mol Cell Biochem Source Type: research

TGF-β-induced hCG-β regulates redox homeostasis in glioma cells.
Abstract Transforming growth factor (TGF-β) is associated with the progression of glioblastoma multiforme (GBM)-the most malignant of brain tumors. Since there is a structural homology between TGF-β and human chorionic gonadotropin (hCG) and as both TGF-β and hCG-β are known regulators of oxidative stress and survival responses in a variety of tumors, the role of TGF-β in the regulation of hCG-β and its consequences on redox modulation of glioblastoma cells was investigated. A heightened hCG-β level was observed in GBM tumors. TGF-β treatment increased hCG-β expression in glioma cell lines, and this heigh...
Source: Molecular and Cellular Biochemistry - October 10, 2014 Category: Biochemistry Authors: Ahmad F, Ghosh S, Sinha S, Joshi SD, Mehta VS, Sen E Tags: Mol Cell Biochem Source Type: research

Notch4 promotes gastric cancer growth through activation of Wnt1/β-catenin signaling.
Abstract Gastric cancer (GC) is one of the most common cancers and lethal malignancies in the world. Discovering novel biomarkers that correlate with GC may provide opportunities to reduce the severity of GC. As one of Notch receptor family members in mammals, Notch4 plays an important role in carcinogenesis of several tumors. However, the precise function and mechanism of Notch4 in GC remain undefined. To address this question, we investigated whether Notch4 could be involved in GC progression. We found that Notch4 was activated by overexpressing exogenous intracellular domain of Notch4 (ICN4), and Notch4 activat...
Source: Molecular and Cellular Biochemistry - December 16, 2014 Category: Biochemistry Authors: Qian C, Liu F, Ye B, Zhang X, Liang Y, Yao J Tags: Mol Cell Biochem Source Type: research

Inhibiting (pro)renin receptor-mediated p38 MAPK signaling decreases hypoxia/reoxygenation-induced apoptosis in H9c2 cells.
In this study, we hypothesized that p38 mitogen-activated protein kinase (MAPK) signaling pathway activation by the (pro)renin receptor had effects on myocardial apoptosis induced by ischemia/reperfusion. This analysis was performed using a hypoxia/reoxygenation model in H9c2 cells to mimic ischemia/reperfusion injury. The H9c2 rat cardiomyocyte cell line was subjected to 2 h of hypoxia followed by 6 h of reoxygenation. The (pro)renin receptor, caspase 3, and phosphorylated p38 MAPK protein expression levels were analyzed by Western blot. After 2 h of hypoxia followed by 6 h of reoxygenation, apoptosis increased in H9c...
Source: Molecular and Cellular Biochemistry - February 25, 2015 Category: Biochemistry Authors: Liu Y, Zhang S, Su D, Liu J, Cheng Y, Zou L, Li W, Jiang Y Tags: Mol Cell Biochem Source Type: research

MiR-21 overexpression improves osteoporosis by targeting RECK.
Abstract Osteoporosis is a kind of metabolic bone disorder. MicroRNA-21 (miR-21) has been proven to play an important role in bone formation, whereas its role in osteoporosis is unclear. In the present study, miR-21 expression was inhibited by TNF-α in mesenchymal stem cells (MSCs). TNF-α induced cell apoptosis, and inhibited cell proliferation and differentiation of MSCs. Whereas the effect was reversed by miR-21 mimics. Expression of reversion-inducing cysteine-rich protein with Kazal motifs (RECK) which is a predicted target of miR-21 was inhibited by miR-21 mimics. A luciferase reporter gene assay showed th...
Source: Molecular and Cellular Biochemistry - April 17, 2015 Category: Biochemistry Authors: Zhao W, Dong Y, Wu C, Ma Y, Jin Y, Ji Y Tags: Mol Cell Biochem Source Type: research

ERK5 signalling pathway is essential for fluid shear stress-induced COX-2 gene expression in MC3T3-E1 osteoblast.
Abstract Bone cells respond to various mechanical stimuli including fluid shear stress (FSS) in vitro. Induction of cyclooxygenase-2 (COX-2) is thought to be important for the anabolic effects of mechanical loading. Recently, extracellular-signal-regulated kinase 5 (ERK5) has been found to be involved in multiple cellular processes. However, the relationship between ERK5 and the induction of COX-2 is still unknown. Here, we investigated the potential involvement of ERK5 in the response of pre-osteoblastic MC3T3-E1 cells upon FSS. MC3T3-E1 cells were subjected to 12 dyn/cm(2) FSS. Then, we established a ERK5 small ...
Source: Molecular and Cellular Biochemistry - May 15, 2015 Category: Biochemistry Authors: Jiang J, Zhao LG, Teng YJ, Chen SL, An LP, Ma JL, Wang J, Xia YY Tags: Mol Cell Biochem Source Type: research

B cell translocation gene 2 (Btg2) is regulated by Stat3 signaling and inhibits adipocyte differentiation.
Abstract Btg2, a member of a family of antiproliferative proteins, is involved in downregulation of the JAK2-Stat3 signaling pathway. Here, we present evidence that the inhibitory effect of Btg2 on adipogenesis is suppressed by the proadipogenic activity of the Stat3 signaling pathway. Btg2 expression fluctuates during adipogenic differentiation of preadipocytes. Btg2 is also expressed at different levels in fat tissues from lean and obese mice. Furthermore, knockdown of Btg2 expression enhanced lipid accumulation and upregulated the expression of adipogenic marker genes. To gain insights into the molecular mechan...
Source: Molecular and Cellular Biochemistry - January 6, 2016 Category: Biochemistry Authors: Kim S, Hong JW, Park KW Tags: Mol Cell Biochem Source Type: research

MEK/ERK pathway activation by insulin receptor isoform alteration is associated with the abnormal proliferation and differentiation of intestinal epithelial cells in diabetic mice.
Abstract In previous studies, we have reported the abnormal proliferation and differentiation of intestinal epithelial cells (IECs) in diabetes mellitus (DM) mice. The insulin receptor (IR) and its downstream mitogen-activated protein kinase kinase (MAPKK also known as MEK)/extracellular-regulated protein kinase (ERK) pathway is a classic pathway associated with cell proliferation and differentiation. The purpose of the present study is to investigate the role of the MEK/ERK pathway in abnormal proliferation and differentiation of IECs in DM mice. DM mouse models were induced by intraperitoneal injection of strept...
Source: Molecular and Cellular Biochemistry - January 2, 2016 Category: Biochemistry Authors: Ouyang H, Yang HS, Yu T, Shan TD, Li JY, Huang CZ, Zhong W, Xia ZS, Chen QK Tags: Mol Cell Biochem Source Type: research

The role of cytochrome P450 1B1 and its associated mid-chain hydroxyeicosatetraenoic acid metabolites in the development of cardiac hypertrophy induced by isoproterenol.
In conclusion, our study provides the first evidence that CYP1B1 and its associated mid-chain HETE metabolites are directly involved in the ISO-induced cardiac hypertrophy. PMID: 28251434 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - February 28, 2017 Category: Biochemistry Authors: Maayah ZH, Althurwi HN, El-Sherbeni AA, Abdelhamid G, Siraki AG, El-Kadi AO Tags: Mol Cell Biochem Source Type: research

CD137-CD137L interaction modulates neointima formation and the phenotype transformation of vascular smooth muscle cells via NFATc1 signaling.
In conclusion, the CD137-CD137L pathway plays an important role in regulating VSMC phenotype transformation via activation of NFATc1 signaling pathway. PMID: 28770466 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - August 2, 2017 Category: Biochemistry Authors: Zhong W, Li B, Yang P, Chen R, Wang C, Wang Z, Shao C, Yuan W, Yan J Tags: Mol Cell Biochem Source Type: research