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Source: Molecular and Cellular Biochemistry

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Total 162 results found since Jan 2013.

Methotrexate affects HMGB1 expression in rheumatoid arthritis, and the downregulation of HMGB1 prevents rheumatoid arthritis progression.
This study examined whether HMGB1 might be involved in the treatment of RA using MTX. Synovial tissues were collected from RA patients who were treated with MTX for at least 6 months (RA-MTX group, 7 cases) and from those without MTX treatment (RA-noMTX group, 7 cases). Additionally, patients with osteoarthritis (OA group, 7 cases) were used as controls. The expression and locations of HMGB1 in the tissues were detected using real-time PCR, western blot, and immunohistochemistry. Additionally, OA-fibroblast-like synoviocytes (FLSs) and RA-FLSs were isolated and cultured, and the expression of HMGB1 was reduced in these ce...
Source: Molecular and Cellular Biochemistry - August 12, 2016 Category: Biochemistry Authors: Li YB, Xu P, Xu K, Cai YS, Sun MY, Yang L, Sun J, Lu SM Tags: Mol Cell Biochem Source Type: research

Forskolin regulates retinal endothelial cell permeability through TLR4 actions in vitro
Mol Cell Biochem. 2021 Aug 28. doi: 10.1007/s11010-021-04252-9. Online ahead of print.ABSTRACTTo investigate whether forskolin, a protein kinase A agonist, regulates toll-like receptor 4 actions on retinal endothelial cell permeability in vitro. We also evaluated whether PKA could regulate TLR4 signaling independent of exchange protein activated by cAMP in REC in culture. REC were grown in normal (5 mM) or high (25 mM) glucose. Cells were treated with forskolin to increase PKA levels, siRNA against TLR4, siRNA against myeloid differentiation primary response 88, siRNA against translocating chain associated membrane protein...
Source: Molecular and Cellular Biochemistry - September 9, 2021 Category: Biochemistry Authors: Li Liu Youde Jiang Jena J Steinle Source Type: research

Concerted inhibition of HIF-1α and -2α expression markedly suppresses angiogenesis in cultured RPE cells.
Abstract HIF-1α is known to play an important role in the induction of VEGF by hypoxia in retinal pigment epithelial (RPE) cells. However, the involvement of the other isoform, HIF-2α, in RPE cells remains unclear. Thus, the purpose of present study was to clarify the role of HIF-2α during induction of angiogenic genes in hypoxic RPE cells. When human RPE cells (ARPE-19) were cultured under hypoxic conditions, HIF-1α and HIF-2α proteins increased. This induced an increase in mRNA for VEGF, causing secretion of VEGF protein into the medium. This conditioned medium induced tube formation in human vascular endot...
Source: Molecular and Cellular Biochemistry - July 20, 2013 Category: Biochemistry Authors: Nakajima T, Nakajima E, Shearer TR, Azuma M Tags: Mol Cell Biochem Source Type: research

Repair of radiation damage of U2OS osteosarcoma cells is related to DNA-dependent protein kinase catalytic subunit (DNA-PKcs) activity.
In conclusion, DNA-PKcs siRNA might have a potential for osteosarcoma treatment by sensitizing osteosarcoma cells to γ-ray through modulation on oxidative stress-mediated DNA DSBs repair and mitochondrial pathway apoptosis. PMID: 24390088 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - January 5, 2014 Category: Biochemistry Authors: Tang X, Yuan F, Guo K Tags: Mol Cell Biochem Source Type: research

DNA-PKcs deficiency sensitizes the human hepatoma HepG2 cells to cisplatin and 5-fluorouracil through suppression of the PI3K/Akt/NF-κB pathway.
In conclusion, DNA-PKcs suppression had complementary effects in combination with CDDP and 5-Fu treatment in HepG2 cells, which was associated with suppression of NF-κB signaling pathway cascade, activation of caspase-3 and p53, as well as down-regulation of Bcl-2 and GSH. PMID: 25348361 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - October 28, 2014 Category: Biochemistry Authors: Fang Y, Chai Z, Wang D, Kuang T, Wu W, Lou W Tags: Mol Cell Biochem Source Type: research

Heat shock protein 60 negatively regulates the biological functions of ubiquitin-like protein MNSF β in macrophages.
In this study, we purified a 65 kDa MNSFβ adduct from mouse liver lysates by sequential chromatography on DEAE and glutathione S-transferase (GST)-fusioned MNSFβ immobilized on glutathione-Sepharose beads in the presence of ATP. MALDI-TOF mass spectrometry fingerprinting revealed that this MNSFβ adduct consists of an 8.5 kDa MNSFβ and heat shock protein 60 (HSP60), a mitochondrial protein involved in protein folding. Fingerprinting analysis of the MNSFβ adduct demonstrates that MNSFβ conjugates to HSP60 with a linkage between the C-terminal Gly74 and Lys481. HSP60 siRNA neutralized the inhibition of apoptosis by MN...
Source: Molecular and Cellular Biochemistry - February 1, 2019 Category: Biochemistry Authors: Nakamura M, Notsu K, Nakagawa M Tags: Mol Cell Biochem Source Type: research

miR-21 modulates chemosensitivity of tongue squamous cell carcinoma cells to cisplatin by targeting PDCD4.
In this study, we investigated the effects and molecular mechanisms of miR-21 in chemosensitivity of tongue squamous cell carcinoma cells (TSCC) to cisplatin. miR-21 expression was detected in tongue cancer tissue using RT-PCR and PDCD4 protein expression was measured using immunohistochemistry. miR-21 and(or) PDCD4 depleted cell lines were generated using miR-21 inhibitor and(or) siRNA. The viabilities of treated cells were analyzed using MTT assay. RT-PCR was used to detect miR-21 expression and immunoblotting was used to detect protein levels. Cell cycle and apoptosis were analyzed using propidium iodide (PI) staining a...
Source: Molecular and Cellular Biochemistry - March 11, 2014 Category: Biochemistry Authors: Ren W, Wang X, Gao L, Li S, Yan X, Zhang J, Huang C, Zhang Y, Zhi K Tags: Mol Cell Biochem Source Type: research

Pin1 modulates chemo-resistance by up-regulating FoxM1 and the involvements of Wnt/β-catenin signaling pathway in cervical cancer.
Abstract The prolyl isomerase Pin1, which is frequently highly expressed in many different cancers, can directly regulate cell proliferation and the cell cycle. However, the role of Pin1 in chemo-resistance remains to be elucidated in cervical cancer. The purpose of the present study was to investigate the role of Pin1 in the chemo-resistance of cervical cancer. The cisplatin resistance was assessed using the MTT assay. Pin1, FoxM1, β-catenin, Cyclin D1, and c-myc expression levels were detected by RT-qPCR or Western blot. The results showed that Pin1 expression displayed a similar expression pattern with the res...
Source: Molecular and Cellular Biochemistry - January 28, 2016 Category: Biochemistry Authors: Wang T, Liu Z, Shi F, Wang J Tags: Mol Cell Biochem Source Type: research

Naringenin ameliorates hypoxia/reoxygenation-induced endoplasmic reticulum stress-mediated apoptosis in H9c2 myocardial cells: involvement in ATF6, IRE1 α and PERK signaling activation.
In conclusion, these results confirmed that ER stress-mediated apoptosis contributes to the protection effects of naringenin against H/R injury, which is potentially involved in ATF6, IRE1α and PERK signaling activation. PMID: 27785700 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - October 25, 2016 Category: Biochemistry Authors: Tang JY, Jin P, He Q, Lu LH, Ma JP, Gao WL, Bai HP, Yang J Tags: Mol Cell Biochem Source Type: research

Estrogen-independent role of ER α in ovarian cancer progression induced by leptin/Ob-Rb axis.
In conclusion, our findings demonstrated that ERα, but not ERβ, is involved in leptin-induced ovarian cancer in an E2-independent manner, providing new evidence for cancer progression in obesity-associated ovarian cancer. PMID: 31077012 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - May 9, 2019 Category: Biochemistry Authors: Ghasemi A, Saeidi J, Mohtashami M, Hashemy SI Tags: Mol Cell Biochem Source Type: research

MiR-19b-3p attenuates IL-1 β induced extracellular matrix degradation and inflammatory injury in chondrocytes by targeting GRK6.
MiR-19b-3p attenuates IL-1β induced extracellular matrix degradation and inflammatory injury in chondrocytes by targeting GRK6. Mol Cell Biochem. 2019 Jun 21;: Authors: Duan L, Duan D, Wei W, Sun Z, Xu H, Guo L, Wu X Abstract Osteoarthritis (OA) is characterized by degradation of articular cartilage. MiRNAs are involved in the regulation of chondrogenesis and OA. We aimed to investigate effects and mechanisms of miR-19b-3p in regulating chondrocytes viability, cartilage degradation and inflammatory response. Primary chondrocytes were isolated from cartilages in control subjects and patients with OA. ...
Source: Molecular and Cellular Biochemistry - June 20, 2019 Category: Biochemistry Authors: Duan L, Duan D, Wei W, Sun Z, Xu H, Guo L, Wu X Tags: Mol Cell Biochem Source Type: research

PICK1 modulates glycolysis and angiogenesis of hypoxic endothelial cells by regulating iron homeostasis
Mol Cell Biochem. 2023 Jun 27. doi: 10.1007/s11010-023-04795-z. Online ahead of print.ABSTRACTIron accumulation, which is controlled by transferrin receptor 1 (TfR1), modulates hypoxia-inducible factor-1α (HIF-1α) activation and angiogenesis of hypoxic endothelial cells. The study examined the role of protein interacting with C-kinase 1 (PICK1), a scaffold protein containing PDZ domain, in regulating glycolysis and angiogenesis of hypoxic vascular endothelial cells through its potential effect on TfR1, which features a supersecondary structure that interacts with the PDZ domain. Iron chelator deferoxamine and TfR1 siRNA ...
Source: Molecular and Cellular Biochemistry - June 27, 2023 Category: Biochemistry Authors: Yu Cao Hongbo Wang Shuyu Hu Qiaomin Xu Jun Ma Huile Wang Xiangqing Xiong Wantie Wang Liangrong Wang Source Type: research