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Role of curcumin in PLD activation by Arf6-cytohesin1 signaling axis in U46619-stimulated pulmonary artery smooth muscle cells.
Abstract Phospholipase D (PLD) catalyzes the hydrolysis of phosphatidylcholine to produce phosphatidic acid (PA) which in some cell types play a pivotal role in agonist-induced increase in NADPH oxidase-derived [Formula: see text]production. Involvement of ADP ribosylation factor (Arf) in agonist-induced activation of PLD is known for smooth muscle cells of systemic arteries, but not in pulmonary artery smooth muscle cells (PASMCs). Additionally, role of cytohesin in this scenario is unknown in PASMCs. We, therefore, determined the involvement of Arf and cytohesin in U46619-induced stimulation of PLD in PASMCs, an...
Source: Molecular and Cellular Biochemistry - August 5, 2017 Category: Biochemistry Authors: Chakraborti S, Sarkar J, Bhuyan R, Chakraborti T Tags: Mol Cell Biochem Source Type: research

CD137-CD137L interaction modulates neointima formation and the phenotype transformation of vascular smooth muscle cells via NFATc1 signaling.
In conclusion, the CD137-CD137L pathway plays an important role in regulating VSMC phenotype transformation via activation of NFATc1 signaling pathway. PMID: 28770466 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - August 2, 2017 Category: Biochemistry Authors: Zhong W, Li B, Yang P, Chen R, Wang C, Wang Z, Shao C, Yuan W, Yan J Tags: Mol Cell Biochem Source Type: research

Inhibition of protein kinase CK2 sensitizes non-small cell lung cancer cells to cisplatin via upregulation of PML.
In conclusion, inhibiting CK2 can enhance sensitivity of CDDP to NSCLC cancer cells through PML. PMID: 28744813 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - July 25, 2017 Category: Biochemistry Authors: Yang B, Yao J, Li B, Shao G, Cui Y Tags: Mol Cell Biochem Source Type: research

MiRNA-200a induce cell apoptosis in renal cell carcinoma by directly targeting SIRT1.
Abstract Accumulating evidence indicates that microRNAs are implicated in tumor initiation and progression through negatively regulating oncogenes or tumor suppressor genes. In the present study, we report that the expression of miR-200a was significantly lower in renal cell carcinoma (RCC) specimens and RCC cell lines. Restoration of miR-200a suppressed cell growth, arrested cell cycle progression, and promoted cell apoptosis in RCC cell lines. We next used qRT-PCR array technology to identify Sirtuin 1 (SIRT1) as one of the downregulated proteins during miR-200a overexpression in 786-O cells. Following a further...
Source: Molecular and Cellular Biochemistry - July 17, 2017 Category: Biochemistry Authors: Fu H, Song W, Chen X, Guo T, Duan B, Wang X, Tang Y, Huang L, Zhang C Tags: Mol Cell Biochem Source Type: research

Protein kinase CK2 regulates redox homeostasis through NF- κB and Bcl-xL in cardiomyoblasts.
Protein kinase CK2 regulates redox homeostasis through NF-κB and Bcl-xL in cardiomyoblasts. Mol Cell Biochem. 2017 Jun 08;: Authors: Schaefer S, Guerra B Abstract Oxygen consumption is particularly elevated in cardiac cells as they are equipped with a large number of mitochondria and high levels of respiratory chain components. Consequently, production of reactive oxygen species (ROS) is tightly controlled as an imbalance in redox reactions can lead to irreversible cellular damage. siRNA-mediated down-regulation of protein kinase CK2 has been implicated in the accumulation of ROS in cells. The prese...
Source: Molecular and Cellular Biochemistry - June 8, 2017 Category: Biochemistry Authors: Schaefer S, Guerra B Tags: Mol Cell Biochem Source Type: research

Splicing factors differentially expressed in psoriasis alter mRNA maturation of disease-associated EDA+  fibronectin.
In this study, we investigated the role of LUC7L3, PPIG, and SFRS18 in psoriasis and in the mRNA maturation process of fibronectin. Regarding tissue staining experiments, we were able to demonstrate a characteristic distribution of the splicing factors in healthy, psoriatic non-involved and involved epidermis. Moreover, the expression profiles of these SR-rich proteins were found to be very similar in synchronized keratinocytes. Contribution of splicing facwwtors to the EDA+ fibronectin formation was also confirmed: their siRNA silencing leads to altered fibronectin mRNA and protein expression patterns, suggesting the par...
Source: Molecular and Cellular Biochemistry - June 6, 2017 Category: Biochemistry Authors: Szlavicz E, Szabo K, Groma G, Bata-Csorgo Z, Pagani F, Kemeny L, Szell M Tags: Mol Cell Biochem Source Type: research

Ubiquitin-specific protease 14 regulates LPS-induced inflammation by increasing ERK1/2 phosphorylation and NF- κB activation.
Ubiquitin-specific protease 14 regulates LPS-induced inflammation by increasing ERK1/2 phosphorylation and NF-κB activation. Mol Cell Biochem. 2017 Mar 31;: Authors: Liu N, Kong T, Chen X, Hu H, Gu H, Liu S, Chen X, Yang Q, Li A, Xiong X, Zhang Z Abstract Persistent activation of nuclear factor B (NF-κB) is very important in the modulation of macrophages cellular response to microbial infections. The deubiquitinase USP14, which is critical for ubiquitin-mediated proteasomal degradation of proteins, is known to be involved in cancer, neurological diseases, and aging. However, the mechanism by which U...
Source: Molecular and Cellular Biochemistry - March 31, 2017 Category: Biochemistry Authors: Liu N, Kong T, Chen X, Hu H, Gu H, Liu S, Chen X, Yang Q, Li A, Xiong X, Zhang Z Tags: Mol Cell Biochem Source Type: research

The role of cytochrome P450 1B1 and its associated mid-chain hydroxyeicosatetraenoic acid metabolites in the development of cardiac hypertrophy induced by isoproterenol.
In conclusion, our study provides the first evidence that CYP1B1 and its associated mid-chain HETE metabolites are directly involved in the ISO-induced cardiac hypertrophy. PMID: 28251434 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - February 28, 2017 Category: Biochemistry Authors: Maayah ZH, Althurwi HN, El-Sherbeni AA, Abdelhamid G, Siraki AG, El-Kadi AO Tags: Mol Cell Biochem Source Type: research

Knockdown of cardiac Kir3.1 gene with siRNA can improve bradycardia in an experimental sinus bradycardia rat model.
The objective of this study was to explore whether the inhibition of potassium inwardly rectifying channel (Kir3.1) with short interfering RNA (siRNA) can improve bradycardia in an experimental sinus bradycardia rat model. 54 Sprague Dawley (SD) rats were randomly divided into three groups: experimental group, control group, and sham group. Sinus bradycardia model was established in SD rats through chemical ablation of sinoatrial (SA) node with 20% formaldehyde. Variations of Kir3.1 expression at mRNA and protein level were examined with qPCR and Western blotting. Electrocardiograms (ECG) of rats at 3 days and 1, 2, 3, an...
Source: Molecular and Cellular Biochemistry - February 14, 2017 Category: Biochemistry Authors: Li Y, Fu X, Zhang Z, Yu B Tags: Mol Cell Biochem Source Type: research

Manganese supplementation increases adiponectin and lowers ICAM-1 and creatinine blood levels in Zucker type 2 diabetic rats, and downregulates ICAM-1 by upregulating adiponectin multimerization protein (DsbA-L) in endothelial cells.
This study investigates the hypothesis that the beneficial effects of Mn supplementation are mediated by adiponectin and DsbA-L. At 6 weeks of age, Male Zucker diabetic fatty rats (ZDF) were randomly divided into two groups: diabetic controls and Mn-supplemented diabetic rats. Each rat was supplemented with Mn (D+Mn, 16 mg/kg BW) or water (placebo, D+P) daily for 7 weeks by oral gavage. For cell culture studies, Human Umbilical Vein Endothelial Cells (HUVEC) or 3T3L1 adipocytes were pretreated with Mn (0-10 µM MnCl2) for 24 h, followed by high glucose (HG, 25 mM) or normal glucose (5 mM) exposure for another 24 h. M...
Source: Molecular and Cellular Biochemistry - January 12, 2017 Category: Biochemistry Authors: Burlet E, Jain SK Tags: Mol Cell Biochem Source Type: research

Akt/AMPK/mTOR pathway was involved in the autophagy induced by vitamin E succinate in human gastric cancer SGC-7901 cells.
Abstract Vitamin E succinate (VES), a derivative of vitamin E, is a promising cancer chemopreventive agent that inhibits tumor promotion by inducing apoptotic cell death. The effects of VES on autophagy, an intricate programmed process which helps cells survive in some stressed situations by degrading some cytoplasmic material, are unclear. When human gastric cancer cells SCG-7901 were exposed to VES, both the level of microtubule-associated protein 1 light chain 3 and the yeast ATG6 homolog Beclin-1 increased, and related autophagy genes were activated, thereby suggesting that autophagy was induced by VES. We als...
Source: Molecular and Cellular Biochemistry - October 27, 2016 Category: Biochemistry Authors: Yu Y, Hou L, Song H, Xu P, Sun Y, Wu K Tags: Mol Cell Biochem Source Type: research

Naringenin ameliorates hypoxia/reoxygenation-induced endoplasmic reticulum stress-mediated apoptosis in H9c2 myocardial cells: involvement in ATF6, IRE1 α and PERK signaling activation.
In conclusion, these results confirmed that ER stress-mediated apoptosis contributes to the protection effects of naringenin against H/R injury, which is potentially involved in ATF6, IRE1α and PERK signaling activation. PMID: 27785700 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - October 25, 2016 Category: Biochemistry Authors: Tang JY, Jin P, He Q, Lu LH, Ma JP, Gao WL, Bai HP, Yang J Tags: Mol Cell Biochem Source Type: research

Cytokeratin 18 is necessary for initiation of TGF- β1-induced epithelial-mesenchymal transition in breast epithelial cells.
Cytokeratin 18 is necessary for initiation of TGF-β1-induced epithelial-mesenchymal transition in breast epithelial cells. Mol Cell Biochem. 2016 Oct 13; Authors: Jung H, Kim B, Moon BI, Oh ES Abstract During epithelial-mesenchymal transition (EMT), epithelial cells lose key phenotypic markers (e.g., E-cadherin and cytokeratin 18) and acquire mesenchymal markers (e.g., N-cadherin and vimentin). Although the loss of cytokeratin 18 is a hallmark of EMT, the regulatory role of cytokeratin 18 in EMT is not yet fully understood. Here, we report that cytokeratin 18 is involved in the regulation of transfor...
Source: Molecular and Cellular Biochemistry - October 12, 2016 Category: Biochemistry Authors: Jung H, Kim B, Moon BI, Oh ES Tags: Mol Cell Biochem Source Type: research

Basic fibroblast growth factor activates β-catenin/RhoA signaling in pulmonary fibroblasts with chronic obstructive pulmonary disease in rats.
Abstract Chronic obstructive pulmonary disease (COPD) is featured by aberrant extracellular matrix (ECM) deposition. Trigger of the β-catenin/RhoA pathway has been involved in aberrant ECM deposition in several diseases. We investigated WNT signaling activation in primary pulmonary fibroblasts of rats with and without COPD and the function of WNT signaling in pulmonary fibroblast. We evaluated the expression of WNT signaling and the role of β-catenin, using MRC-5 fibroblasts and primary lung fibroblasts of rats with and without COPD. Lung fibroblasts highly expressed mRNA of genes associated with WNT signaling. ...
Source: Molecular and Cellular Biochemistry - October 12, 2016 Category: Biochemistry Authors: Ge Z, Li B, Zhou X, Yang Y, Zhang J Tags: Mol Cell Biochem Source Type: research

Methotrexate affects HMGB1 expression in rheumatoid arthritis, and the downregulation of HMGB1 prevents rheumatoid arthritis progression.
This study examined whether HMGB1 might be involved in the treatment of RA using MTX. Synovial tissues were collected from RA patients who were treated with MTX for at least 6 months (RA-MTX group, 7 cases) and from those without MTX treatment (RA-noMTX group, 7 cases). Additionally, patients with osteoarthritis (OA group, 7 cases) were used as controls. The expression and locations of HMGB1 in the tissues were detected using real-time PCR, western blot, and immunohistochemistry. Additionally, OA-fibroblast-like synoviocytes (FLSs) and RA-FLSs were isolated and cultured, and the expression of HMGB1 was reduced in these ce...
Source: Molecular and Cellular Biochemistry - August 12, 2016 Category: Biochemistry Authors: Li YB, Xu P, Xu K, Cai YS, Sun MY, Yang L, Sun J, Lu SM Tags: Mol Cell Biochem Source Type: research

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