Filtered By:
Source: Molecular and Cellular Biochemistry

This page shows you your search results in order of date. This is page number 3.

Order by Relevance | Date

Total 162 results found since Jan 2013.

Activation of AMPK inhibits Galectin-3-induced pulmonary artery smooth muscle cells proliferation by upregulating hippo signaling effector YAP
Mol Cell Biochem. 2021 Apr 2. doi: 10.1007/s11010-021-04131-3. Online ahead of print.ABSTRACTGalectin-3(Gal-3) is an effective regulator in the pathological process of pulmonary arterial hypertension (PAH). However, the detailed mechanisms underlying Gal-3 contribution to PAH are not yet entirely clear. The aim of the present study was to explore these issues. Proliferation of rat pulmonary arterial smooth muscle cells (PASMCs) was determined using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Small interfering RNA (siRNA) was applied to silence the expression of yes-associated protein (YAP) a...
Source: Molecular and Cellular Biochemistry - April 2, 2021 Category: Biochemistry Authors: Qianqian Zhang Wenge Li Yanting Zhu Qingting Wang Cui Zhai Wenhua Shi Wei Feng Jian Wang Xin Yan Limin Chai Yuqian Chen Cong Li Pengtao Liu Manxiang Li Source Type: research

Omega-3 fatty acid protects cardiomyocytes against hypoxia-induced injury through targeting MiR-210-3p/CASP8AP2 axis
Mol Cell Biochem. 2021 Mar 31. doi: 10.1007/s11010-021-04141-1. Online ahead of print.ABSTRACTMicroRNAs (miRs) regulate diverse biological functions in both normal and pathological cellular conditions by post-transcriptional regulation of various genes expression. Nevertheless, the role of miRs in regulating the protective functions of omega-3 fatty acid in relation to hypoxia in cardiomyocytes remains unknown. The aim of this study was to investigate the effects of omega-3 fatty acid supplementation on cardiomyocyte apoptosis and further delineate the mechanisms underlying microRNA-210 (miRNA-210)-induced cardiomyocyte ap...
Source: Molecular and Cellular Biochemistry - April 1, 2021 Category: Biochemistry Authors: Xueju Yu Fengyao Liu Yuting Liu Bingqing Bai Han Yin Haochen Wang Yingqing Feng Huan Ma Qingshan Geng Source Type: research

SAA1 regulates pro-labour mediators in term labour by activating YAP pathway
In conclusion, these experiments demonstrated that SAA1 accelerates the inflammatory response associated with parturition by activating YAP pathway, which may be a novel understanding of the molecular mechanism of labour onset.PMID:33719002 | DOI:10.1007/s11010-021-04125-1
Source: Molecular and Cellular Biochemistry - March 15, 2021 Category: Biochemistry Authors: Yanmin Jiang Li Pin Weiqun Shi Qian Huang Lele Wang Huishu Liu Source Type: research

KLF9 regulates PRDX6 expression in hyperglycemia-aggravated bupivacaine neurotoxicity.
CONCLUSIONS: The results of this study demonstrated that hyperglycemia aggravated Bup neurotoxicity by upregulating KLF9 expression, which repressed the antioxidant PRDX6 and led to mitochondrial dysfunction, ROS burst, and cell death. Understanding this mechanism may, thus, offer valuable insights for the prevention and treatment of neurotoxicity induced by LAs, especially in diabetic patients. PMID: 33547545 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - February 5, 2021 Category: Biochemistry Authors: Li H, Weng Y, Lai L, Lei H, Xu S, Zhang Y, Li L Tags: Mol Cell Biochem Source Type: research

Activation of STING inhibits cervical cancer tumor growth through enhancing the anti-tumor immune response.
In this study, we found that the cGAS (Cyclic GMP-AMP synthase)/STING signal decreased in cervical cancer cells. Knockdown of STING by siRNA enhanced the cell viability and migration of cervical cancer cells, while activation of STING by ADU-S100 inhibited the cell viability of cervical cancer cells, with no effect on the migration and apoptosis. In addition, ADU-S100 promoted the secretion of IFNβ and IL-6, and the activation of TBK1 (TANK-binding kinase 1)/NF-κB (nuclear factor kappa-B) pathway. Meanwhile, knockdown of STING inhibited the production of IFNβ and IL-6 that were triggered by dsDNA and suppressed the TBK1...
Source: Molecular and Cellular Biochemistry - November 3, 2020 Category: Biochemistry Authors: Shi F, Su J, Wang J, Liu Z, Wang T Tags: Mol Cell Biochem Source Type: research

TAK1 is involved in sodium L-lactate-stimulated p38 signaling and promotes apoptosis.
In this study, we suggested that TAK1 plays an important role in L-lactate-stimulated activation of p38 affecting apoptosis in HeLa cells. PMID: 33111211 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - October 27, 2020 Category: Biochemistry Authors: Da Q, Yan Z, Li Z, Han Z, Ren M, Huang L, Zhang X, Liu J, Wang T Tags: Mol Cell Biochem Source Type: research

Ectopic expression of 35  kDa and knocking down of 78 kDa SG2NAs induce cytoskeletal reorganization, alter membrane sialylation, and modulate the markers of EMT.
Ectopic expression of 35 kDa and knocking down of 78 kDa SG2NAs induce cytoskeletal reorganization, alter membrane sialylation, and modulate the markers of EMT. Mol Cell Biochem. 2020 Oct 20;: Authors: Gupta R, Kumar G, Jain BP, Chandra S, Goswami SK Abstract SG2NA is a protein of the striatin family that organizes STRIPAK complexes. It has splice variants expressing differentially in tissues. Its 78 kDa isoform regulates cell cycle, maintains homeostasis in the endoplasmic reticulum, and prevents oxidative injuries. The 35 kDa variant is devoid of the signature WD-40 repeats in the carboxy termin...
Source: Molecular and Cellular Biochemistry - October 20, 2020 Category: Biochemistry Authors: Gupta R, Kumar G, Jain BP, Chandra S, Goswami SK Tags: Mol Cell Biochem Source Type: research

New perspectives in triple-negative breast cancer therapy based on treatments with TGF β1 siRNA and doxorubicin.
In this study, a combined doxorubicin (DOX) and small interfering RNA (siRNA) therapy is proposed as therapeutic strategy for targeting TGFβ1 gene. Hs578T cell line is used as in vitro model for TNBC, wherein TGFβ1siRNA therapy is employed to enhance therapeutic effects. Cell proliferation rate is measured using an MTT test, and morphological alterations are assed using microscopically approached, while gene expression is determined by qRT-PCR analysis. The combined treatment of TGFβ1siRNA and DOX reduced levels of cell proliferation and mitochondrial activity and promoted the alteration of cell morphology (dark-field m...
Source: Molecular and Cellular Biochemistry - September 3, 2020 Category: Biochemistry Authors: Ciocan-Cȃrtiţă CA, Jurj A, Raduly L, Cojocneanu R, Moldovan A, Pileczki V, Pop LA, Budişan L, Braicu C, Korban SS, Berindan-Neagoe I Tags: Mol Cell Biochem Source Type: research

Isoflurane promotes proliferation of squamous cervical cancer cells through mTOR-histone deacetylase 6 pathway.
In conclusion, Isoflurane enhanced proliferation of cervical cancer cells through upregulation of histone deacetylase 6, which was associated with mTOR-dependent pathway, but not AKT-mediated pathway. PMID: 32833118 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - August 23, 2020 Category: Biochemistry Authors: Zhang W, Xue F, Xie S, Chen C, Li J, Zhu X Tags: Mol Cell Biochem Source Type: research

Speedy/RINGO protein interacts with ERK/MAPK and PI3K/AKT pathways in SH-SY5Y neuroblastoma cells.
This study provides information about a possible interaction of Speedy/RINGO with PI3K/AKT and ERK/MAPK pathways in SH-SY5Y cells for the first time. It will not only help to better understand the cancer-prone interactions of these pathways but also enable us to identify the appropriate molecular targets for developing efficient treatment strategies. PMID: 32602013 [PubMed - as supplied by publisher]
Source: Molecular and Cellular Biochemistry - June 28, 2020 Category: Biochemistry Authors: Kaya Y, Kucukvardar S, Yildiz A Tags: Mol Cell Biochem Source Type: research

Growth factor receptor bound protein-7 regulates proliferation, cell cycle, and mitochondrial apoptosis of thyroid cancer cells via MAPK/ERK signaling.
Abstract It is of great significance to explore the molecular mechanism of thyroid cancer (TC) pathogenesis for its improvement and therapy. Growth factor receptor bound protein-7 (GRB7) has been regarded as an important regulatory gene in the developments of various malignant tumors. Our study aimed to illustrate the role of GRB7 in the TC pathology mechanism. Firstly, GRB7 was found to be significantly upregulated in 49 cases of TC tissues and 5 TC cell lines by using real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting. Silencing GRB7 with siRNA dramatically inhibited proliferation an...
Source: Molecular and Cellular Biochemistry - June 22, 2020 Category: Biochemistry Authors: Tang H, Yang P, Yang X, Peng S, Hu X, Bao G Tags: Mol Cell Biochem Source Type: research

A novel function of IRF9 in acute pancreatitis by modulating cell apoptosis, proliferation, migration, and suppressing SIRT1-p53.
This study was aimed to explore the role and mechanism of interferon regulatory factor 9 (IRF9) in the occurrence of AP and to provide experimental and theoretical foundation for AP diagnosis and treatment. AP model in vitro was established by caerulein-induced group. Small interfering RNA (siRNA) was designed and constructed to silence IRF9 gene. After siRNA transfected and caerulein treated successfully, the expression levels of IRF9, SIRT1, and acetylated p53 (Ac-p53) were determined by qRT-PCR and Western blot. The apoptosis, proliferation, and migration of AR42J cells were checked by flow cytometry, MTT, and transwell...
Source: Molecular and Cellular Biochemistry - June 22, 2020 Category: Biochemistry Authors: Xue BH, Liu Y, Chen H, Sun Y, Yu WL Tags: Mol Cell Biochem Source Type: research

Bcl-2/Bcl-xL inhibitor navitoclax increases the antitumor effect of Chk1 inhibitor prexasertib by inducing apoptosis in pancreatic cancer cells via inhibition of Bcl-xL but not Bcl-2.
Abstract In our previous study, we showed that prexasertib, a checkpoint kinase 1 (Chk1) inhibitor, enhances the effects of standard drugs for pancreatic cancer, including gemcitabine (GEM), S-1, and the combination of GEM and S-1 (GS). The combination of prexasertib and GS has a strong antitumor effect and induces apoptosis in pancreatic cancer cells by downregulating anti-apoptotic protein Bcl-2. In the present study, we investigated the combined effect of GEM, S-1, and prexasertib with a selective Bcl-2 inhibitor (venetoclax) and a non-selective Bcl-2 inhibitor (navitoclax) in SUIT-2 pancreatic cancer cells. An...
Source: Molecular and Cellular Biochemistry - June 20, 2020 Category: Biochemistry Authors: Morimoto Y, Takada K, Takeuchi O, Watanabe K, Hirohara M, Hamamoto T, Masuda Y Tags: Mol Cell Biochem Source Type: research

ILK silencing inhibits migration and invasion of more invasive glioblastoma cells by downregulating ROCK1 and Fascin-1.
In this study, we used two brain cell lines, the non-invasive neuroglioma H4 cells, and the highly invasive glioblastoma A172 cells, which express ILK in much higher levels than H4. We studied the effect of ILK silencing on the metastatic behavior of glioblastoma cells in vitro and elucidate the underlying molecular mechanism. We showed that siRNA-mediated silencing of ILK inhibits cell migration and invasion of the highly invasive A172 cells while it does not affect the migratory and invasive capacity of H4 cells. These data were also supported by respective changes in the expression of Rho-associated kinase 1 (ROCK1), fa...
Source: Molecular and Cellular Biochemistry - June 5, 2020 Category: Biochemistry Authors: Louca M, Zaravinos A, Stylianopoulos T, Gkretsi V Tags: Mol Cell Biochem Source Type: research