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Total 93 results found since Jan 2013.

The role of NRG1 in the predisposition to papillary thyroid carcinoma.
Conclusions: Our data suggest a role for transcriptional regulation of NRG1 in the predisposition to PTC. PMID: 29121253 [PubMed - as supplied by publisher]
Source: The Journal of Clinical Endocrinology and Metabolism - November 7, 2017 Category: Endocrinology Authors: He H, Li W, Liyanarachchi S, Wang Y, Yu L, Genutis LK, Maharry S, Phay JE, Shen R, Brock P, de la Chapelle A Tags: J Clin Endocrinol Metab Source Type: research

Mixed lineage kinase domain-like protein induces RGC-5 necroptosis following elevated hydrostatic pressure.
Abstract Receptor-interacting protein 3 (RIP3) is an essential component of the necroptosis signaling pathway. Phosphorylation of its downstream target, mixed lineage kinase domain-like protein (MLKL), has been proposed to induce necroptosis by initiating Ca2+ influx. Our previous studies have shown that RGC-5 retinal ganglion cells undergo RIP3-mediated necroptosis following elevated hydrostatic pressure (EHP). However, the molecular mechanism underlying necroptosis induction downstream of RIP3 is still not well understood. Here, we investigated the effects of MLKL during EHP-induced necroptosis, and primarily ex...
Source: Acta Biochimica et Biophysica Sinica - October 1, 2017 Category: Biochemistry Authors: Liao L, Shang L, Li N, Wang S, Wang M, Huang Y, Chen D, Huang J, Xiong K Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

RAMP2 influences glucagon receptor pharmacology via trafficking and signaling.
Abstract Endogenous satiety hormones provide an attractive target for obesity drugs. Glucagon causes weight loss by reducing food intake and increasing energy expenditure. To further understand the cellular mechanisms by which glucagon and related ligands activate the glucagon receptor (GCGR), we have investigated the interaction of the GCGR with RAMP2, a member of the family of Receptor Activity Modifying Proteins.We have used a combination of competition binding experiments, cell surface ELISA, functional assays assessing the Gαs and Gq pathways and β-arrestin recruitment, and siRNA knockdown to examine the ef...
Source: Endocrinology - June 6, 2017 Category: Endocrinology Authors: Cegla J, Jones BJ, Gardiner JV, Hodson DJ, Marjot T, McGlone ER, Tan TM, Bloom SR Tags: Endocrinology Source Type: research

The histone acetylation mediated by Gcn5 regulates the Hoxc11 gene expression in MEFs.
In this study, we propose to investigate the role of Gcn5, a histone acetyltransferase, in the regulation of Hoxc11 expression in MEFs. We showed that the H3 lysine 9 acetylation (H3K9ac) status has the same correlation with Hoxc11 expression and reported that Gcn5 is associated with the upregulation of Hoxc11 expression through H3K9ac in Akt1-/- MEFs. Since Hoxc11 was upregulated through histone acetylation in Akt1-/- MEFs, a functional role of Gcn5 on Hoxc11 expression was analyzed in Akt1-/- MEFs treated with Gcn5 specific inhibitor or transfected with Gcn5-small interfering RNA (Gcn5-siRNA). When the expression of Hoxc...
Source: Acta Biochimica et Biophysica Sinica - May 24, 2017 Category: Biochemistry Authors: Oh JH, Lee JY, Kong KA, Kim JM, Kim MH Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

hCG activates Epac-Erk1/2 signaling regulating Progesterone Receptor expression and function in human endometrial stromal cells
AbstractSTUDY QUESTIONHow does hCG signal in human endometrial stromal cells (ESCs) and what is its role in regulating ESC function?SUMMARY ANSWERhCG signaling in ESCs activates the extracellular signal-regulated protein kinases 1 and 2 (Erk1/2) pathway through exchange protein activated by cyclic AMP (cAMP) (Epac) and transiently increases progesterone receptor (PR) transcript and protein expression and its transcriptional function.WHAT IS KNOWN ALREADYhCG is one of the earliest embryo-derived secreted signals in the endometrium, which abundantly expresses LH/hCG receptors. hCG signals through cAMP/protein kinase A (PKA) ...
Source: Molecular Human Reproduction - March 28, 2017 Category: Molecular Biology Source Type: research

The adequate corpus luteum: miR-96 promotes luteal cell survival and progesterone production.
Conclusions: miR-96 targets FOXO1 to regulate luteal development through effects on cell survival and steroid production. The miR-183-96-182 cluster could provide a novel target for the manipulation of luteal function. PMID: 28368475 [PubMed - as supplied by publisher]
Source: The Journal of Clinical Endocrinology and Metabolism - March 20, 2017 Category: Endocrinology Authors: Mohammed BT, Sontakke SD, Ioannidis J, Duncan WC, Donadeu FX Tags: J Clin Endocrinol Metab Source Type: research

Hypoxia-inducible lipid droplet-associated (HILPDA) is not a direct physiological regulator of lipolysis in adipose tissue.
Abstract Triglycerides are stored in specialized organelles called lipid droplets. Numerous proteins have been shown to be physically associated with lipid droplets and govern their function. Previously, hypoxia-inducible lipid droplet-associated (HILPDA) was localized to lipid droplets and was suggested to inhibit triglyceride lipolysis in hepatocytes. We confirm the partial localization of HILPDA to lipid droplets and show that HILPDA is highly abundant in adipose tissue, where its expression is controlled by the peroxisome proliferator-activated receptor γ and by β-adrenergic stimulation. Levels of HILPDA mar...
Source: Endocrinology - March 17, 2017 Category: Endocrinology Authors: Dijk W, Mattijssen F, de la Rosa Rodriguez M, Loza Valdes A, Loft A, Mandrup S, Kalkhoven E, Qi L, Borst JW, Kersten S Tags: Endocrinology Source Type: research

Intracellular Delivery by Shape Anisotropic Magnetic Particle-Induced Cell Membrane Cuts
We report a novel cell delivery platform based on rotating shape anisotropic magnetic particles (SAMPs), which make very small cuts on cell membranes for macromolecule delivery with high efficiency and high survivability. SAMP delivery is performed by placing commercially available nickel powder onto cells grown in standard cell culture dishes. Application of a uniform magnetic field causes the magnetic particles to rotate because of mechanical torques induced by shape anisotropic magnetization. Cells touching these rotating particles are nicked, which generates transient membrane pores that enable the delivery of macromol...
Source: Journal of Laboratory Automation - July 14, 2016 Category: Laboratory Medicine Authors: Lin, M.-Y., Wu, Y.-C., Lee, J.-A., Tung, K.-W., Zhou, J., Teitell, M. A., Yeh, J. A., Chiou, P. Y. Tags: Original Research Source Type: research

Current preclinical small interfering RNA (siRNA)-based conjugate systems for RNA therapeutics.
Abstract Recent promising clinical results of RNA therapeutics have drawn big attention of academia and industries to RNA therapeutics and their carrier systems. To improve their feasibility in clinics, systemic evaluations of currently available carrier systems under clinical trials and preclinical studies are needed. In this review, we focus on recent noticeable preclinical studies and clinical results regarding siRNA-based conjugates for clinical translations. Advantages and drawbacks of siRNA-based conjugates are discussed, compared to particle-based delivery systems. Then, representative siRNA-based conjugate...
Source: Advanced Drug Delivery Reviews - October 27, 2015 Category: Drugs & Pharmacology Authors: Lee SH, Kang YY, Jang HE, Mok H Tags: Adv Drug Deliv Rev Source Type: research

Genome wide screening of RNAi factors of Sf21 cells reveal several novel pathway associated proteins
Conclusion: The database of RNAi-factors generated by whole genome association survey offers comprehensive outlook about conservation as well as specific differences of the proteins of RNAi machinery. Understanding the interior involved in different phases of gene silencing also offers impending tool for RNAi-based applications.
Source: BMC Genomics - Latest articles - September 9, 2014 Category: Genetics & Stem Cells Authors: Subhanita GhoshPavan KakumaniAjit KumarPawan MalhotraSunil MukherjeeRaj Bhatnagar Source Type: research

Activin A, B and AB increase human trophoblast cell invasion by up-regulating N-cadherin.
Conclusion: Activin A, B and AB produce comparable increases in human trophoblast cell invasion by up-regulating N-cadherin expression in a SMAD2/3-SMAD4-dependent manner. PMID: 25105734 [PubMed - as supplied by publisher]
Source: The Journal of Clinical Endocrinology and Metabolism - August 8, 2014 Category: Endocrinology Authors: Li Y, Klausen C, Cheng JC, Zhu H, Leung PC Tags: J Clin Endocrinol Metab Source Type: research

PI3K/AKT pathway mediates induction of IL-1RA by TSH in fibrocytes: Modulation by PTEN.
Conclusions: The current findings identify PI3K/AKT pathway as critical to the induction by TSH of IL-1RA in fibrocytes and GD-OF. Further PTEN modulates the amplitude of the induction. In GD-OF, relatively high basal PTEN levels prevent sIL-1RA expression or release of IL-1RA. Knocking down PTEN allows GD-OF to exhibit a pattern of IL-1RA expression resembling fibrocytes. PMID: 24840811 [PubMed - as supplied by publisher]
Source: The Journal of Clinical Endocrinology and Metabolism - May 19, 2014 Category: Endocrinology Authors: Li B, Smith TJ Tags: J Clin Endocrinol Metab Source Type: research

TGF-β1 induces COX-2 expression and PGE2 production in human granulosa cells through Smad signaling pathways.
Conclusion: TGF-β1 induced PGE2 production by inducing COX-2 expression through a Smad-dependent signaling pathway in human granulosa cells. PMID: 24712567 [PubMed - as supplied by publisher]
Source: The Journal of Clinical Endocrinology and Metabolism - April 8, 2014 Category: Endocrinology Authors: Fang L, Chang HM, Cheng JC, Leung PC, Sun YP Tags: J Clin Endocrinol Metab Source Type: research

Expression of thyrotropin receptor, thyroglobulin, sodium-iodide symporter, and thyroperoxidase by fibrocytes depends on AIRE.
Conclusions: Fibrocytes express 4 proteins, the aggregate expression of which was previously thought to be restricted to thyroid epithelium. These proteins represent the necessary molecular biosynthetic machinery necessary for thyroid hormone production. Our findings implicate AIRE in the promiscuous expression of thyroid proteins in fibrocytes. PMID: 24708100 [PubMed - as supplied by publisher]
Source: Diabetes Metab - April 7, 2014 Category: Endocrinology Authors: Fernando R, Lu Y, Atkins SJ, Mester T, Branham K, Smith TJ Tags: J Clin Endocrinol Metab Source Type: research

The High Throughput Biomedicine unit at the Institute for Molecular Medicine Finland: High throughput screening meets precision medicine.
Abstract The High Throughput Biomedicine (HTB) unit at the Institute for Molecular Medicine Finland FIMM was established in 2010 to serve as a national and international academic screening unit providing access to state of the art instrumentation for chemical and RNAi-based high throughput screening. The initial focus of the unit was multiwell plate based chemical screening and high content microarray-based siRNA screening. However, over the first four years of operation, the unit has moved to a more flexible service platform where both chemical and siRNA screening is done at different scales primarily in multiwel...
Source: Combinatorial Chemistry and High Throughput Screening - March 23, 2014 Category: Chemistry Authors: Pietiäinen V, Saarela J, Schantz-Fant CV, Turunen L, Ostling P, Wennerberg K Tags: Comb Chem High Throughput Screen Source Type: research